Intranuclear distribution of SV40 large T-antigen and transformation-related protein p53 in abortively infected cells.

Abstract:

:The intranuclear localization of SV40 T-antigen (T-Ag) and the cellular protein p53 was studied in SV40 abortively infected baby mouse kidney cells using two complementary methods of ultrastructural immunocytochemistry in combination with preferential staining of nuclear RNP components and electron microscope autoradiography. Both proteins were revealed in association with peri- and interchromatin RNP fibrils containing the newly synthesized hnRNA. In addition, T-Ag and p53 remained bound, at least in part, to the residual internal nuclear matrix following nuclease and salt extractions of infected cells. The localization of T-Ag was different in SV40 lytically infected monkey kidney cells since, in addition to hnRNP fibrils, the viral protein was also associated with cellular chromatin. However, when lytic infection was performed in conditions of blocked viral DNA replication, T-Ag was no longer associated with the cellular chromatin but remained bound to the hnRNP fibrils. We conclude that the transforming and lytic functions of T-Ag can be distinguished by different subnuclear distributions. The significance of the association of T-Ag and p53 with hnRNP fibrils and the internal nuclear matrix is discussed in relation to the role of these structures in the control of cellular mRNA biogenesis.

journal_name

Exp Cell Res

authors

Puvion E,Duthu A,Harper F,Ehrhart JC,Viron A,May P

doi

10.1016/0014-4827(88)90026-2

subject

Has Abstract

pub_date

1988-07-01 00:00:00

pages

73-89

issue

1

eissn

0014-4827

issn

1090-2422

pii

0014-4827(88)90026-2

journal_volume

177

pub_type

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