Abstract：
:Evidence is presented that endonuclease digestion of isolated, unfixed chromosomes results in the production of banding patterns similar to those produced by digestion of fixed, air-dried chromosomes. Mouse L cell chromosomes were isolated under acidic or relatively neutral pH conditions, exposed in situ (as wet mounts on glass slides) or in vitro (in suspension) to micrococcal nuclease, Alu I or Eco RI, treated with a buffered salt solution, and stained with Giemsa. After any of these endonuclease treatments in situ, the centromeric regions of the chromosomes were intensely stained, characteristic of the C-banding observed in fixed chromosomes exposed to the same treatments. Although the fixed chromosomes were morphologically well-preserved after endonuclease digestion, the morphology of chromosomes digested in situ was variable, ranging from normal to swollen to highly distorted chromosomes. In the latter, the endonucleases induced dispersion of non-C-band chromatin; however, C-bands were still apparent as condensed, differentially-stained regions. Exposure of isolated chromosomes to Alu I in vitro also resulted in well-defined C-banding and led to the extraction of about 70% of the chromosomal DNA. From these results, the mechanism of endonuclease-induced C-banding appears to involve the dispersion and extraction of digested chromatin.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Burkholder GD,Schmidt GJdoi
10.1016/0014-4827(86)90036-4subject
Has Abstractpub_date
1986-06-01 00:00:00pages
379-87issue
2eissn
0014-4827issn
1090-2422journal_volume
164pub_type
杂志文章abstract：:Individual tumor cells utilize one of two modes of motility to invade the extracellular matrix, mesenchymal or amoeboid. We have determined that the diterpenoid genkwanine M (GENK) enhances the mesenchymal mode of cell motility that is intrinsic to HT-1080 osteosarcoma cells, stimulates a mesenchymal mode of motility ...
journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2013-04-01 00:00:00
abstract：:We have investigated the role played by the growth and differentiation factor (GDF)-induced calcium ion second messenger signal in the control of cellular differentiation and proliferation in Syrian hamster embryo (SHE) cells. Blocking the platelet-derived growth factor (PDGF)-induced calcium ion signal with either ex...
journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1996-08-01 00:00:00
abstract：:An attempt was made to determine the role of metabolic acidification of pericellular medium in regulating cell proliferation. A method of measuring the pH of pericellular medium at a distance exceeding a Debye radius of 5-10 A from the cell surface (pHp) was developed. The values of pHp and pH measured in the medium a...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(85)90188-0
更新日期：1985-10-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(91)90156-o
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abstract：:Nuclear-envelope nucleoside triphosphatase activity (NTPase), an enzymatic activity thought to participate in RNA transport, was localized in rat liver in situ after brief perfusion with 3% paraformaldehyde. Reaction product was distributed along the nucleoplasmic side of the nuclear envelope (NE) in heterochromatin, ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90796-1
更新日期：1984-11-01 00:00:00
abstract：:This study reports on the specific binding of [3H]heparin to human adrenocortical carcinoma cell line SW-13. Heparin binding to SW-13 cells is specific, saturable, and time- and temperature-dependent with maximum binding occurring between 90 and 120 min at 22 degrees C. Scatchard analysis revealed two classes of bindi...
journal_title：Experimental cell research
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更新日期：1990-04-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1989-08-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.3394
更新日期：1997-02-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1999-05-25 00:00:00
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90460-x
更新日期：1986-05-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2015-08-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2020.112217
更新日期：2020-10-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：
更新日期：1996-07-10 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.10.032
更新日期：2006-02-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(83)90015-0
更新日期：1983-05-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90393-m
更新日期：1992-02-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90031-5
更新日期：1986-06-01 00:00:00
abstract：:In studies of the ontogeny of fibroblast-epithelial interactions during late fetal lung rat lung development, we have identified two subpopulations of fibroblasts which differed in their ability to promote epithelial cell proliferation or differentiation. As glycosaminoglycans (GAGs) have been implicated in the regula...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90072-g
更新日期：1992-10-01 00:00:00
abstract：:HIV-associated nephropathy (HIVAN) is characterized by heavy proteinuria, rapidly progressive renal failure, and distinct morphological features in the kidney. HIV-induced epithelial-mesenchymal transition (EMT) is critically important for the progression of kidney injury. In this study, we tested the role of hedgehog...
journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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更新日期：2005-09-10 00:00:00
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journal_title：Experimental cell research
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更新日期：2000-02-01 00:00:00
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1993.1016
更新日期：1993-01-01 00:00:00
abstract：:Many cell types secrete plasma membrane-bound microvesicles, suggested to play an important role in tissue morphogenesis, wound healing, and cancer spreading. However, the mechanisms of their formation have remained largely unknown. It was found that the tips of long microvilli induced in cells by overexpression of hy...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2013.05.021
更新日期：2013-08-01 00:00:00
