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An altered repertoire of fos/jun (AP-1) at the onset of replicative senescence.

Abstract：

:With multiple divisions in culture, normal diploid cells suffer a loss of growth potential that leads to replicative senescence and a finite replicative capacity. Using quantitative RT-PCR, we have monitored mRNA expression levels of c-fos, c-jun, JunB, c-myc, p53, H-ras, and histone H4 during the replicative senescence of human fibroblasts. The earliest and the largest changes in gene expression occurred in c-fos and junB at mid-senescence prior to the first slowing in cell growth rates. The basal level of c-fos mRNA decreased to one-ninth that of the early-passage levels, while junB declined to one-third and c-jun expression remained constant. The decline in the basal c-fos mRNA level in mid-senescence should lead to an increase in Jun/Jun AP-1 homodimers at the expense of Fos/Jun heterodimers and may trigger a cascade of further changes in c-myc, p53, and H-ras expression in late-passage senescent fibroblasts.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Irving J,Feng J,Wistrom C,Pikaart M,Villeponteau B

doi

10.1016/0014-4827(92)90415-5

keywords:

subject

Has Abstract

pub_date

1992-09-01 00:00:00

pages

161-6

issue

1

eissn

0014-4827

issn

1090-2422

pii

0014-4827(92)90415-5

journal_volume

202

pub_type

杂志文章

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