Abstract:
:Liver cells of new-born rats, which were found to be able to form spheroidal aggregates when cultured on a nonadherent plastic substratum, were studied under various conditions of culture, mainly by adding different nutrients and growth factors to the culture medium. Analysis of hepatocyte-specific functions was carried out by immunoprecipitation to detect specific proteins newly secreted by liver cell spheroids on different days of culture. When no supplement was added to culture medium, the secretion of albumin and transferrin by liver cell spheroids was no longer detectable after 2 weeks of culture. When dexamethasone, glucagon, insulin, and EGF were added to culture medium, the secretion of albumin and transferrin remained detectable at least until 60 days of culture. This was even more striking when trace elements were added in addition to the three hormones and EGF. The effects of addition of these various factors to culture medium were also detectable with respect to alpha-FP secretion. Even after 54 days of culture in total supplemented medium, these liver cell spheroids could be transferred on a collagen-coated plastic substratum to form a monolayer of uniform liver parenchyma-like cells. The presence of extracellular matrix-like material was observed on the surface of cell spheroids. This could be responsible for attachment and fusion between cell spheroids. Thus, liver cell spheroids cultured in total supplemented medium ensured cell attachment to a biological matrix and cell-cell contact, which is thought to help maintain cell differentiation. Liver cell spheroids offer the possibility of toxicological and pharmacological studies as well as cultures in biomatrix and coculture systems. In addition these liver cells can be used for experiments in liver cell transplantation.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Tong JZ,Bernard O,Alvarez Fdoi
10.1016/0014-4827(90)90260-hsubject
Has Abstractpub_date
1990-07-01 00:00:00pages
87-92issue
1eissn
0014-4827issn
1090-2422pii
0014-4827(90)90260-Hjournal_volume
189pub_type
杂志文章abstract::Infection with Kaposi's sarcoma-associated herpesvirus (KSHV) is a prerequisite for Kaposi's sarcoma, a multicentric endothelial tumor of mixed blood vessel-lymphatic phenotype with a marked production of endothelial basement membrane proteins. The viral K1 gene encodes a unique transmembrane protein capable of transf...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2003.09.010
更新日期:2004-01-15 00:00:00
abstract::Ran, a nuclear GTPase, and a number of interacting proteins, including regulators RanGEF1 and RanGAP1, are involved in nucleocytoplasmic transport. We have identified a new temperature-sensitive mutation in budding yeast YRB1 gene, which encodes Ran-binding protein-1 (RanBP1). In contrast to other yrb1 alleles, the ne...
journal_title:Experimental cell research
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doi:10.1006/excr.1998.4174
更新日期:1998-10-10 00:00:00
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pub_type: 杂志文章
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journal_title:Experimental cell research
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更新日期:2018-03-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章,评审
doi:10.1016/j.yexcr.2008.07.025
更新日期:2009-02-15 00:00:00
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journal_title:Experimental cell research
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更新日期:1991-07-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2000.4865
更新日期:2000-05-25 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2014.05.010
更新日期:2014-09-10 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
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更新日期:2004-01-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1998.3939
更新日期:1998-04-10 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.yexcr.2016.02.004
更新日期:2016-02-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2004.12.017
更新日期:2005-04-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(88)90347-3
更新日期:1988-11-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1993.1263
更新日期:1993-10-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1994.1258
更新日期:1994-09-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2002.5512
更新日期:2002-05-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2008.05.008
更新日期:2008-08-01 00:00:00
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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doi:10.1006/excr.2001.5242
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4566
更新日期:1999-08-25 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/s0014-4827(03)00316-1
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(85)90257-5
更新日期:1985-01-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1996.3464
更新日期:1997-02-25 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(85)90471-9
更新日期:1985-06-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2012.08.008
更新日期:2012-12-10 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2003.08.015
更新日期:2004-01-01 00:00:00
abstract::In this study, a somatic cell genetic approach was used to study the regulation of liversolidusbonesoliduskidney alkaline phosphatase (ALPL) gene expression in osteoblasts. ALPL plays an important role in skeletal mineralization and serves as a good index of bone formation. A series of intertypic hybrids constructed b...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:
更新日期:1996-07-10 00:00:00