SV40 large T antigen reinduces the cell cycle in terminally differentiated myotubes through inducing Cdk2, Cdc2, and their partner cyclins.


:Terminally differentiated skeletal muscle myotubes are arrested in G0 phase of the cell cycle and are unable to be released from this arrest by stimulation with mitogens including serum and growth factors. To inspect a possibility of reversing the quiescence at the G0 phase, we have exploited the mouse skeletal muscle cell line C2SVTts11, which is a clone of C2 cells transfected with the SV40 T antigen gene (encoding thermolabile large T and wild-type small t) fused to an inducible promoter. When the large T is induced in the myotubes, the terminally differentiated cells reenter the cell cycle and proceed to S and M phases. To elucidate how large T forces the myotubes to traverse each phase of the cell cycle, we examined the expression and activity of Cdk2 and Cdc2, which in complex with cyclin A and cyclin B are essential for S and M phases, respectively in undifferentiated cells. The levels of their mRNAs and proteins and histone H1 kinase activity, which was ascribed to Cdc2-cyclin B, were high in the proliferating myoblasts but gradually decreased during terminal differentiation. In contrast, they were reinduced in the myotubes reentering the cell cycle. Stimulation of the myotubes with serum failed to evoke these factors. These results indicate that large T, but not mitogens, is able to drive terminally differentiated myotubes to pass each phase of the cell cycle through eliciting these factors as do mitogens on proliferating undifferentiated cells. Since large T is a nuclear protein, signals generated by the protein in the nucleus are likely to be sufficient to induce each phase of the cell cycle in the terminally differentiated cells.


Exp Cell Res


Ohkubo Y,Kishimoto T,Nakata T,Yasuda H,Endo T




Has Abstract


1994-09-01 00:00:00














  • 1,25-Dihydroxyvitamin D3 and Transforming Growth Factor-beta Act Synergistically to Override Extinction of LiversolidusBonesolidusKidney Alkaline Phosphatase in Osteosarcoma Hybrid Cells

    abstract::In this study, a somatic cell genetic approach was used to study the regulation of liversolidusbonesoliduskidney alkaline phosphatase (ALPL) gene expression in osteoblasts. ALPL plays an important role in skeletal mineralization and serves as a good index of bone formation. A series of intertypic hybrids constructed b...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Johnson-Pais TL,Leach RJ

    更新日期:1996-07-10 00:00:00

  • Epigenetic and HIF-1 regulation of stanniocalcin-2 expression in human cancer cells.

    abstract::Mammalian stanniocalcin-2 (STC2) is a secreted glycoprotein hormone with a putative role in unfolded protein response and apoptosis. Here we reported that STC2 expression was sporadically abrogated in human cancer cells by transcriptional silencing associated with CpG island promoter hypermethylation. Direct sequencin...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Law AY,Lai KP,Ip CK,Wong AS,Wagner GF,Wong CK

    更新日期:2008-05-01 00:00:00

  • The S-phase cytotoxicity of camptothecin.

    abstract::The DNA topoisomerase I inhibitor camptothecin (CAM) is selectively cytotoxic to S-phase cells of HL-60, and some other myelogenous leukemic lines. The early effects of cell exposure to 0.05-0.2 micrograms/ml CAM are seen after 2 h; at that time a progressive degradation of DNA in the chromatin of S-phase cells is ini...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Del Bino G,Lassota P,Darzynkiewicz Z

    更新日期:1991-03-01 00:00:00

  • Protein degradation and apoptotic death in lymphocytes during Fiv infection: activation of the ubiquitin-proteasome proteolytic system.

    abstract::The movement of a cell through the sequential phases of apoptosis is accompanied by a progressive decrease in cell size with loss in protein mass. In lymphocytes from Hiv-infected persons, protein loss during apoptosis is due to increased protein degradation rather than decreased synthesis. To identify and characteriz...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Piedimonte G,Crinelli R,Della Salda L,Corsi D,Pennisi MG,Kramer L,Casabianca A,Sarli G,Bendinelli M,Marcato PS,Magnani M

    更新日期:1999-05-01 00:00:00

  • Resistance of Chinese hamster ovary cell chromatin to endonuclease digestion. I. Reversal by cAMP.

    abstract::A portion of the DNA within intact nuclei of a spontaneously transformed Chinese hamster ovary cell line (CHO-Kl) is relatively resistant to digestion by pancreatic deoxyribonuclease, as compared to nuclei from primary cultures of Chinese hamster ovary fibroblasts. Treatment of CHO-Kl cells with derivatives of 3',5' c...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Schonberg S,Patterson D,Puck TT

    更新日期:1983-04-15 00:00:00

  • Exemption of satellite DNA from demethylation in immortalized differentiated derivatives of F9 mouse embryonal carcinoma cells.

    abstract::DNA methylation in F9 embryonal carcinoma cells at various stages of retinoic acid-induced differentiation was compared to that in immortalized differentiated derivatives of this cell line. Different repetitive sequences, such as L1 LINE, GAPDH pseudogenes, and major and minor satellite DNA, lost their methylation to ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Teubner B,Schulz WA

    更新日期:1994-02-01 00:00:00

  • An enhancer required for transcription of the Col6a1 gene in muscle connective tissue is induced by signals released from muscle cells.

    abstract::Collagen VI is a survival factor for skeletal muscle produced by endomysial cells and localized in connective tissue around muscle fibers. Mutations of its genes (COL6A1, COL6A2 and COL6A3) cause two muscular disorders, Bethlem myopathy and Ullrich disease. Expression of Collagen VI is highly dynamic during developmen...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Braghetta P,Ferrari A,Fabbro C,Bizzotto D,Volpin D,Bonaldo P,Bressan GM

    更新日期:2008-11-15 00:00:00

  • Organization of early and late replicating DNA in human chromosome territories.

    abstract::It has been suggested that DNA organized into replication foci during S-phase remains stably aggregated in non-S-phase cells and that these stable aggregates provide fundamental units of nuclear or chromosome architecture [C. Meng and R. Berezney (1991) J. Cell Biol. 115, 95a; E. Sparvoli et al. (1994) J. Cell Sci. 10...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zink D,Bornfleth H,Visser A,Cremer C,Cremer T

    更新日期:1999-02-25 00:00:00

  • The Schrödinger's cat quandary in cell biology: integration of live cell functional assays with measurements of fixed cells in analysis of apoptosis.

    abstract::The existing cytometric methodologies do not allow one to directly correlate, within the same cells, functional cell attributes that are revealed supravitally with features that require cell fixation to be detected or measured. Taking advantage of the "file merge" feature of the laser-scanning cytometer, we have been ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Li X,Darzynkiewicz Z

    更新日期:1999-06-15 00:00:00

  • Serum effects on the in vitro differentiation of sea urchin micromeres.

    abstract::When micromeres isolated from the 16-cell stage of Strongylocentrotus purpuratus are cultured in sea water containing 3.5% horse serum, they produce spicules at approximately the same time as in normal development. The serum requirement of the micromeres has been investigated by adding serum at varying intervals after...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: McCarthy RA,Spiegel M

    更新日期:1983-12-01 00:00:00

  • Different strategies of X-inactivation in germinal and somatic cells: histone H4 underacetylation does not mark the inactive X chromosome in the mouse male germline.

    abstract::It has previously been shown by immunocytochemistry that the inactive X chromosome (Xi) in somatic cells of human and mouse females is marked by underacetylation of histone H4. It has been suggested that this may be important for transcriptional silencing of genes on Xi. We have now investigated X-inactivation in meio...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Armstrong SJ,Hultén MA,Keohane AM,Turner BM

    更新日期:1997-02-01 00:00:00

  • Dopamine 1 receptor activation protects mouse diabetic podocytes injury via regulating the PKA/NOX-5/p38 MAPK axis.

    abstract::Diabetic nephropathy (DN) is a major microvascular complication of diabetes that can lead to end-stage renal disease. Podocytes constitute the last barrier of glomerular filtration, whose damage are the direct cause of proteinuria. Dopamine receptors are involved in the regulation of diabetes-induced glomerular hyperf...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Shao X,Zhang X,Hu J,Gao T,Chen J,Xu C,Wei C

    更新日期:2020-03-15 00:00:00

  • Growth-stimulatory action of plasma membrane-associated growth factor. A synergistic effect with platelet-poor plasma.

    abstract::Growth factor activity was partially purified from mouse liver plasma membranes and its growth-stimulatory action on cultured mouse fibroblasts was studied. The plasma membrane-associated growth factor (PMGF) was unable to support the proliferation of mouse fibroblasts in monolayer when added as the sole source of gro...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Nagasaki T,Lieberman MA

    更新日期:1987-05-01 00:00:00

  • Possible role of deep tubular invaginations of the plasma membrane in MHC-I trafficking.

    abstract::Tubules and vesicles are membrane carriers involved in traffic along the endocytic and secretory routes. The small GTPase Arf6 regulates a recycling branch of short dynamic tubular intermediates used by major histocompatibility class I (MHC-I) molecules to traffic through vesicles between endosomes and the plasma memb...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Massol RH,Larsen JE,Kirchhausen T

    更新日期:2005-05-15 00:00:00

  • Hypomethylation of human heterochromatin detected by restriction enzyme nick translation.

    abstract::Using the restriction enzymes MspI and HpaII in the nick translation procedure it has been shown that decondensation of the paracentric heterochromatin of chromosome 9 during human spermatogenesis is associated with hypomethylation of the DNA sequences in this domain. Somatic cells treated with 5'-azacytidine also sho...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Mitchell AR

    更新日期:1992-09-01 00:00:00

  • Transforming NRK cells with avian sarcoma virus reduces the extracellular Ca2+ requirement without affecting the calcicalmodulin requirement for the G1/S transition.

    abstract::NRK rat cells infected with a transformation-defective, temperature-sensitive (ts) mutant of the avian sarcoma virus could not proliferate in Ca2+-deficient medium at a nonpermissive temperature (40 degrees C) that inactivated the viral pp60v-src-transforming product and rendered the cells phenotypically untransformed...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Durkin JP,Whitfield JF

    更新日期:1985-04-01 00:00:00

  • siRNA knockdown of alanine aminopeptidase impairs myoblast proliferation and differentiation.

    abstract::A large number of intracellular proteins are degraded by the ubiquitin-proteasome system, one of the major protein degradation pathways. It produces peptides of several different sizes through protein degradation, and these peptides are rapidly degraded into free amino acids by various intracellular aminopeptidases. P...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Osana S,Kitajima Y,Suzuki N,Xu Y,Murayama K,Nagatomi R

    更新日期:2020-12-01 00:00:00

  • Cardiac apoptosis caused by elevated cholesterol level in experimental autoimmune myocarditis.

    abstract::It has been reported that cholesterol-lowing agents can ameliorate severity of myocarditis. However, the beneficial effect of the agents has been claimed to be independent of cholesterol reduction as there is no significant change in the plasma cholesterol level in myocarditis. In the present study, using experimental...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Chang H,Wang Y,Wu Y,Ma P,Song Y,Liu C,Ye Y,Qi JH,Qi Z

    更新日期:2020-10-01 00:00:00

  • Parallels between immune driven-hematopoiesis and T cell activation: 3 signals that relay inflammatory stress to the bone marrow.

    abstract::Quiescence, self-renewal, lineage commitment and differentiation of hematopoietic stem cells (HSCs) towards fully mature blood cells are a complex process that involves both intrinsic and extrinsic signals. During steady-state conditions, most hematopoietic signals are provided by various resident cells inside the bon...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Libregts SF,Nolte MA

    更新日期:2014-12-10 00:00:00

  • Membrane glycoproteins involved in hepatocyte adhesion to collagen type I.

    abstract::Liver membrane glycoproteins with affinity for immobilized collagen type I were subjected to preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by electroelution of the separated proteins. Electroeluted glycoproteins with ability to neutralize the inhibitory effect of anti-CollCA...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Gullberg D,Terracio L,Rubin K

    更新日期:1988-04-01 00:00:00

  • Subcellular distribution, secretion, and posttranslational modifications of clusterin in thyrocytes.

    abstract::A prominent secretory glycoprotein was detected in the culture medium of porcine thyrocytes which was identified as clusterin by microsequencing. Treatment of thyrocytes with thyroid stimulating hormone revealed a tight regulation of both synthesis and secretion of clusterin, with a distinct fraction of clusterin bein...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lemansky P,Brix K,Herzog V

    更新日期:1999-08-25 00:00:00

  • Functional characterization of the microtubule-binding and -destabilizing domains of CPAP and d-SAS-4.

    abstract::We previously identified a novel centrosomal protein CPAP, which carries a 112-residue motif that is essential for microtubule destabilization. In this report, we define both the microtubule (MT) binding and destabilizing domains in human CPAP and analyze the mutations that affect its MT-destabilizing activity. Analys...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hsu WB,Hung LY,Tang CJ,Su CL,Chang Y,Tang TK

    更新日期:2008-08-15 00:00:00

  • Vectorial transcytosis of immunoglobulin G by human term trophoblast cells in culture.

    abstract::Trophoblast cells were grown on filters that allow access to apical and basal surfaces of cells. Using this experimental system, IgG transport was shown to be specific and to occur primarily in the apical to basal direction. This transport was time- and temperature-dependent, with approximately 10% of added IgG appear...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Sooranna SR,Contractor SF

    更新日期:1991-01-01 00:00:00

  • Absence of the Shb gene in mixed-lineage leukemia MLL-AF9 cells increases latency in mice despite higher proliferation rates in vitro.

    abstract::Mixed lineage leukemia (MLL) arises from several KMT2A-gene chromosomal translocations. Shb gene deficiency has been found to exhibit pleiotropic effects in different models of leukemia, and consequently, this study aimed to investigate MLL-AF9-induced leukemia in Shb deficiency. Bone marrow cells from wild type and S...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Jamalpour M,Bergquist E,Welsh M

    更新日期:2020-12-15 00:00:00

  • Specific modulation of surface receptors in J.774 macrophages by anchorage.

    abstract::The J.774 murine macrophage cells were cultured in suspension in Teflon flasks. When allowed to attach on culture plastic dishes, a 2-3-fold increase in transferrin binding was observed. This occurred in 10 min, reached a steady state at 60 min, remained stable for several hours and was reversible after resuspension o...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ventura MA,Louache F,Rouis M,Erlich D,Goldstein S,Testa U,Thomopoulos P

    更新日期:1987-06-01 00:00:00

  • Downregulation of endogenous STAT3 augments tumoricidal activity of interleukin 15 activated dendritic cell against lymphoma and leukemia via TRAIL.

    abstract::Effector functions in tumor resistance by dendritic cells (DCs) are less well characterized. In this study, we describe that the murine DCs upon stimulation with recombinant IL-15 in vitro or in vivo, expresses TNF superfamily member TRAIL which mediates cytotoxicity and growth inhibition against a murine lymphoma cal...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hira SK,Mondal I,Bhattacharya D,Manna PP

    更新日期:2014-10-01 00:00:00

  • Roles of bHLH genes in neural stem cell differentiation.

    abstract::Neural stem cells change their characteristics over time during development: they initially proliferate only and then give rise to neurons first and glial cells later. In the absence of the repressor-type basic helix-loop-helix (bHLH) genes Hes1, Hes3 and Hes5, neural stem cells do not proliferate sufficiently but pre...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Kageyama R,Ohtsuka T,Hatakeyama J,Ohsawa R

    更新日期:2005-06-10 00:00:00

  • The comparison of DNA synthesis inducibility by high potassium treatment in various nonproliferating cells.

    abstract::Previous studies have demonstrated that treatment of fibroblasts from confluent, density-inhibited cultures with 50 mM KCl solution led to the onset of DNA replication. In the present study we show that such treatment can induce aphidicolin-sensitive DNA replication in differentiated nondividing cells: dorsal root gan...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Fedorov YV,Prudovsky IA,Yegorov YE,Zelenin AV

    更新日期:1993-11-01 00:00:00

  • Recombinant human IgG antibodies recognizing distinct extracellular domains of EGF receptor exhibit different degrees of growth inhibitory effects on human A431 cancer cells.

    abstract::Recently, we isolated 4 distinct kinds of single chain antibody against human EGF receptor (EGFR) after screening the Keio phage display scFv library by using two methods of target-guided proximity labeling. In the current study, these monovalent scFv antibodies were converted to bivalent IgGs of humanized forms (hIgG...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Chang C,Takayanagi A,Yoshida T,Shimizu N

    更新日期:2013-05-01 00:00:00

  • Actin cytoskeleton organization, cell surface modification and invasion rate of 5 glioblastoma cell lines differing in PTEN and p53 status.

    abstract::Glioblastoma cells exhibit highly invasive behavior whose mechanisms are not yet fully understood. The present study explores the relationship between the invasion capacity of 5 glioblastoma cell lines differing in p53 and PTEN status, expression of mTOR and several other marker proteins involved in cell invasion, act...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Djuzenova CS,Fiedler V,Memmel S,Katzer A,Hartmann S,Krohne G,Zimmermann H,Scholz CJ,Polat B,Flentje M,Sukhorukov VL

    更新日期:2015-01-15 00:00:00