Abstract：
:Rafoxanide is commonly used as anti-helminthic medicine in veterinary medicine, a main compound of salicylanilide. Previous studies have reported that rafoxanide, as an inhibitor of BRAF V600E mutant protein, inhibits the growth of colorectal cancer, multiple myeloma, and skin cancer. However, its therapeutic effect on gastric cancer (GC) and the potential mechanism has not been investigated. Here, we have found that rafoxanide inhibited the proliferation of GC cells in vitro, arrested the cell cycle in the G0/G1 phase, and promoted apoptosis and autophagy in GC cells. Treatment with specific autophagy inhibitor 3-methyladenine drastically inhibited the apoptotic cell death effect by suppressing the switch from autophagy to apoptosis. Mechanistically, we found that rafoxanide inhibited the growth of GC cells in vitro by inhibiting the activity of the PI3K/Akt/mTOR signaling pathway. This process induced autophagy, which essentially resulted in the apoptosis of GC cells. Results from subcutaneous implanted tumor models in nude mice also indicated that rafoxanide inhibited the growth of GC cells in vivo. Taken together, our findings revealed that rafoxanide inhibited the growth of GC cells both in vitro and vivo, indicating a potential drug candidate for the treatment of GC.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Liu JZ,Hu YL,Feng Y,Guo YB,Liu YF,Yang JL,Mao QS,Xue WJdoi
10.1016/j.yexcr.2019.111691subject
Has Abstractpub_date
2019-12-15 00:00:00pages
111691issue
2eissn
0014-4827issn
1090-2422pii
S0014-4827(19)30565-8journal_volume
385pub_type
杂志文章abstract：:Over-activation of beta-catenin/TCF signaling has been frequently observed in hepatocellular carcinoma (HCC). Better understanding the molecular mechanism for the aberrant activation of beta-catenin/TCF signaling would provide novel insights into the treatment of this malignancy. In this study, we have shown that the ...
journal_title：Experimental cell research
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abstract：:Galectin-3, a beta-galactoside-binding protein, has been shown to be involved in multiple biological processes through interaction with its complementary glycoconjugates. Here we provide the first evidence of galectin-3 as a mitogen. Incubation of quiescent cultures of normal human lung fibroblast IMR-90 cells with re...
journal_title：Experimental cell research
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doi：10.1016/j.yexcr.2007.12.017
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
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doi：10.1006/excr.1998.4027
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0271
更新日期：1996-09-15 00:00:00
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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pub_type： 杂志文章
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journal_title：Experimental cell research
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