TGF-beta receptors, in a Smad-independent manner, are required for terminal skeletal muscle differentiation.

abstract：:There are a number of low-abundance small nuclear RNAs (snRNAs) in eukaryotic cells. Many of them have been assigned functions in the biogenesis of cellular RNAs, such as splicing and 3' end processing. Here, we present the sequence of Xenopus U12 snRNA and compare the secondary structures of the low-abundance U11 and...

journal_title：Experimental cell research

authors： Yu YT,Tarn WY,Yario TA,Steitz JA

更新日期：1996-12-15 00:00:00

abstract：:The MUC6 mucin has a critical protective function in the normal stomach, pancreas and duodenum and is aberrantly expressed during the progression of some gastrointestinal cancers. Our aim was to determine whether MUC6 contributes to the etiology or progression of pancreatic cancer and elucidate the molecular basis of ...

journal_title：Experimental cell research

authors： Leir SH,Harris A

更新日期：2011-10-15 00:00:00

abstract：:Numerous factors that promote movement of macromolecules in and out of the nucleus have now been identified. These include both soluble cytoplasmic and nucleoplasmic proteins and proteins of the nuclear pore complex (NPC). Genetic analyses of the nuclear transport process in the model organism, the budding yeast Sacch...

journal_title：Experimental cell research

authors： Corbett A,Ferrigno P,Henry M,Kahana J,Koepp D,Lee M,Nguyen L,Schlenstedt G,Seedorf M,Shen E,Taura T,Wong D,Silver P

更新日期：1996-12-15 00:00:00

abstract：:Sertoli cells of testis belong to a unique type of polarized epithelial cells and are essential for spermatogenesis. They form the blood-testis barrier at the base of seminiferous tubule. Their numerous long, microtubule-rich processes extend inward and associate with developing germ cells to sustain germ cell growth ...

journal_title：Experimental cell research

authors： Wang F,Zhang Q,Cao J,Huang Q,Zhu X

更新日期：2008-01-01 00:00:00

abstract：:FTO, a demethylase for N6-methyladenosine (m6A) modification, has been implicated in multiple tumors. However, its roles in esophageal squamous cell carcinoma (ESCC) remain uncovered. This study aims to evaluate the clinical relevance and functional roles in this disease. Through immunohistochemistry, qRT-PCR and West...

journal_title：Experimental cell research

authors： Liu S,Huang M,Chen Z,Chen J,Chao Q,Yin X,Quan M

更新日期：2020-04-01 00:00:00

abstract：:Inhibition of p38MAPK alpha/beta is known to enhance 1,25-dihydroxyvitamin (1,25D)-induced monocytic differentiation, but the detailed mechanism of this effect was not clear. We now show that the enhancement of differentiation becomes apparent with slow kinetics (12-24 h). Interestingly, the inhibition of p38MAPK alph...

journal_title：Experimental cell research

authors： Zhang J,Harrison JS,Studzinski GP

更新日期：2011-01-01 00:00:00

abstract：:Histone deacetylase inhibitors (HDACi) were recently identified as having significant clinical potential in reversing β-cell functional inhibition caused by inflammation, a shared precursor of Type 1 and Type 2 diabetes. However, HDACi are highly complex and little is known of their direct effect on important cell sec...

journal_title：Experimental cell research

authors： Tiernan AR,Champion JA,Sambanis A

更新日期：2015-01-01 00:00:00

abstract：:The bone marrow represents an easy accessible source of adult stem cells suitable for various cell based therapies. Several studies in recent years suggested the existence of pluripotent stem cells within bone marrow stem cells (BMSC) expressing marker proteins of both embryonic and tissue committed stem cells. These ...

journal_title：Experimental cell research

authors： Sauerzweig S,Munsch T,Lessmann V,Reymann KG,Braun H

更新日期：2009-01-01 00:00:00

abstract：:Studies performed over the past five years have demonstrated that there are two major cell-intrinsic pathways for inducing apoptosis, one that begins with ligation of cell surface death receptors and another that involves mitochondrial release of cytochrome c. Several reports have suggested that anticancer drugs kill ...

journal_title：Experimental cell research

authors： Kaufmann SH,Earnshaw WC

更新日期：2000-04-10 00:00:00

abstract：:MicroRNAs (miRNAs) are a class of small non-coding RNAs that repress the expression of their target genes post-transcriptionally. MiRNAs participate in the regulation of a variety of biological processes, including development and diseases. However, the functional role and molecular mechanism by which miRNAs regulate ...

journal_title：Experimental cell research

authors： Wang L,Chen X,Zheng Y,Li F,Lu Z,Chen C,Liu J,Wang Y,Peng Y,Shen Z,Gao J,Zhu M,Chen H

更新日期：2012-11-01 00:00:00

abstract：:Interleukin 3 (IL-3) stimulates the growth of various types of hemopoietic progenitors. In vitro, survival of a series of murine cell lines derived from either neoplastic or nonneoplastic hemopoietic tissue shows a strict IL-3 dependence. In order to test the implication of energy metabolism in this dependence as clai...

journal_title：Experimental cell research

authors： Reisbach G,Ellwart J,Dörmer P

更新日期：1990-10-01 00:00:00

abstract：:The role of the F-actin cytoskeleton in cell volume regulation was studied in Ehrlich ascites tumor cells, using a quantitative rhodamine-phalloidin assay, confocal laser scanning microscopy, and electronic cell sizing. A hypotonic challenge (160 mOsm) was associated with a decrease in cellular F-actin content at 1 an...

journal_title：Experimental cell research

authors： Pedersen SF,Mills JW,Hoffmann EK

更新日期：1999-10-10 00:00:00

abstract：:A single cell of wild-type Dictyostelium discoideum forms a visible colony on a plastic dish in several days, but due to enhanced cell migration, amiB-null mutant cells scatter over a large area and do not form noticeable colonies. Here, with an aim to identify genes involved in cell migration, we isolated suppresser ...

journal_title：Experimental cell research

authors： Nagasaki A,Uyeda TQ

更新日期：2008-03-10 00:00:00

abstract：:Considering that stimulation of melanogenesis may lead to alterations of cellular responses, besides melanin production, our main goal was to study the cellular effects of melanogenesis stimulation of B16-F10 melanoma cells. Our results show increased levels of the reactive oxygen species after 15 h of melanogenesis s...

journal_title：Experimental cell research

authors： Cunha ES,Kawahara R,Kadowaki MK,Amstalden HG,Noleto GR,Cadena SM,Winnischofer SM,Martinez GR

更新日期：2012-09-10 00:00:00

abstract：:A new beta 1-containing integrin was isolated from rat liver by affinity chromatography on Sepharose conjugated with the peptide GRGDSPC. The interaction was weakened but not abolished when the arginine and/or aspartic acid in the peptide were replaced with lysine and glutamic acid, respectively. In contrast, the cyst...

journal_title：Experimental cell research

authors： Forsberg E,Ek B,Engström A,Johansson S

更新日期：1994-07-01 00:00:00

abstract：:During skeletal muscle development three intermediate filament proteins are expressed: nestin, vimentin, and desmin. Vimentin and desmin belong to the class III intermediate filaments and are closely related to each other, whereas nestin is a more distantly related, class VI, intermediate filament. It was previously o...

journal_title：Experimental cell research

authors： Sjöberg G,Jiang WQ,Ringertz NR,Lendahl U,Sejersen T

更新日期：1994-10-01 00:00:00

abstract：:Thyroid hormone (T3) and estradiol (Est) modulate biological processes by binding to nuclear receptor proteins that, through interactions with specific response elements in the regulatory regions of genes, modulate gene transcription. Est stimulation of estrogen receptor (ER)-positive breast carcinoma cell growth occu...

journal_title：Experimental cell research

authors： Shao ZM,Sheikh MS,Rishi AK,Dawson MI,Li XS,Wilber JF,Feng P,Fontana JA

更新日期：1995-05-01 00:00:00

abstract：:We have investigated the role played by the growth and differentiation factor (GDF)-induced calcium ion second messenger signal in the control of cellular differentiation and proliferation in Syrian hamster embryo (SHE) cells. Blocking the platelet-derived growth factor (PDGF)-induced calcium ion signal with either ex...

journal_title：Experimental cell research

authors： Isfort RJ,Cody DB,Stuard SB,Ridder GM,LeBoeuf RA

更新日期：1996-08-01 00:00:00

abstract：:Analyses of mice with targeted deletions in the genes for alpha3 and beta1 integrin suggest that the alpha3beta1 integrin heterodimer likely determines the organization of the extracellular matrix within the basement membrane of skin. Here we tested this hypothesis using keratinocytes derived from alpha3 integrin-null...

journal_title：Experimental cell research

authors： deHart GW,Healy KE,Jones JC

更新日期：2003-02-01 00:00:00

abstract：:TGFβ contributes to mesangial cell hypertrophy and matrix protein increase in various kidney diseases including diabetic nephropathy. Deptor is an mTOR-interacting protein and suppresses mTORC1 and mTORC2 activities. We have recently shown that TGFβ-induced inhibition of deptor increases the mTOR activity. The mechani...

journal_title：Experimental cell research

authors： Maity S,Bera A,Ghosh-Choudhury N,Das F,Kasinath BS,Choudhury GG

更新日期：2018-03-01 00:00:00

abstract：:The osteoblast-like MC3T3-E1 cell line provides an excellent in vitro model of bone development. This system undergoes three orderly time-dependent phases characterized by proliferating preosteoblasts, matrix accumulation by postmitotic differentiating osteoblasts, and mineralization of the matrix, which results in th...

journal_title：Experimental cell research

authors： Vary CP,Li V,Raouf A,Kitching R,Kola I,Franceschi C,Venanzoni M,Seth A

更新日期：2000-05-25 00:00:00

abstract：:The dynamics of lamin disassembly and reassembly during sea urchin male pronuclear development in vitro was investigated. Using five anti-lamin antibodies, we monitored by immunofluorescence and immunoblotting the changes in lamins during sperm chromatin decondensation, nuclear envelope (NE) formation, and male pronuc...

journal_title：Experimental cell research

authors： Collas P,Pinto-Correia C,Poccia DL

更新日期：1995-08-01 00:00:00

abstract：:To measure the adhesion of cells expressing the neural cell adhesion molecule N-CAM, mouse Lmtk- fibroblast cells were transfected by a calcium phosphate precipitation technique with eucaryotic expression vectors encoding N-CAM polypeptides. We obtained cell lines expressing the 140-kDa transmembrane isoform of N-CAM ...

journal_title：Experimental cell research

authors： Woo MK,Murray BA

更新日期：1993-02-01 00:00:00

abstract：:The androgen receptor (AR) plays critical roles in human prostate carcinoma progression and transformation. However, the activation of AR is regulated by co-regulators. MEIS1 protein, the homeodomain transcription factor, exhibited a decreased level in poor-prognosis prostate tumors. In this study, we investigated a p...

journal_title：Experimental cell research

authors： Cui L,Li M,Feng F,Yang Y,Hang X,Cui J,Gao J

更新日期：2014-10-15 00:00:00

abstract：:The human embryonal carcinoma (EC) cell line Tera 2 clone 13 (T2/13) can be induced to differentiate in vitro into neuroectodermal cell types with retinoic acid. Undifferentiated cells are characterized by rapid proliferation, whereas differentiated cells show a prolonged generation time, have a limited life span, and...

journal_title：Experimental cell research

authors： Weima SM,van Rooijen MA,Mummery CL,Feyen A,de Laat SW,van Zoelen EJ

更新日期：1990-02-01 00:00:00

abstract：:Paromomycin is an aminocyclitol aminoglycoside antibiotic used for the treatment of leishmaniasis. In view of the central role of mitochondria in cellular energetics and metabolism, its effect on in vivo mitochondrial activities of Leishmania donovani promastigotes-the parasite flagellate form-was investigated. The ap...

journal_title：Experimental cell research

authors： Maarouf M,de Kouchkovsky Y,Brown S,Petit PX,Robert-Gero M

更新日期：1997-05-01 00:00:00

abstract：:The abilities of eight extracellular matrix proteins, fibronectin, vitronectin, laminin, and collagen types I, II, III, IV, and V to bind insulin were examined by binding studies with insulin conjugated with peroxidase. At a physiological pH and ionic strength, type V collagen bound to insulin most strongly. The other...

journal_title：Experimental cell research

authors： Yaoi Y,Hashimoto K,Takahara K,Kato I

更新日期：1991-06-01 00:00:00

abstract：:Rat IPC-81 promyelocytic leukemia cells responded to cAMP analog by undergoing apoptotic cell death both when anchored to fibronectin and when free in the medium. The protein kinase C stimulator 12-O-tetradecanoylphorbol 13-acetate enhanced the anchoring to substratum without impeding cAMP-induced cell death. The immo...

journal_title：Experimental cell research

authors： Vintermyr OK,Gjertsen BT,Lanotte M,Døskeland SO

更新日期：1993-05-01 00:00:00

abstract：:A temperature-sensitive mutant, tsJT60, grew exponentially at both 34 degrees and 39.5 degrees C, but when stimulated from the resting state it entered S phase at 34 degrees but not at 39.5 degrees C. The mutated function appeared to be a prerequisite throughout from 0 to 9 h following the stimulation, in order that G...

journal_title：Experimental cell research

authors： Ninomiya-Tsuji J,Goto Y,Ishibashi S,Ide T

更新日期：1986-07-01 00:00:00

abstract：:Rat myotubes in tissue culture form broad areas of close contact with the substrate. These areas often display two distinct, interdigitating sets of membrane domains. One, the "contact domain", is close to the substrate; the other, termed the "AChR domain", is further from the substrate and is rich in acetylcholine re...

journal_title：Experimental cell research

authors： Bloch RJ,Velez M,Krikorian JG,Axelrod D

更新日期：1989-06-01 00:00:00