24R,25-dihydroxyvitamin D3 increases cyclic GMP contents, leading to an enhancement of osteocalcin synthesis by 1,25-dihydroxyvitamin D3 in cultured human osteoblastic cells.

abstract：:The program of biochemical and molecular events necessary for commitment to erythroid cell differentiation is particularly well characterized in murine Friend erythroleukemia cell lines. Commitment to hemoglobin synthesis in response to a variety of chemical inducers, including hexamethylene bisacetamide and dimethyl ...

journal_title：Experimental cell research

authors： Mallia CM,Aguirre V,McGary E,Tang Y,Scandurro AB,Liu C,Noguchi CT,Beckman BS

更新日期：1999-02-01 00:00:00

abstract：:Calf aortic smooth muscle (CASM) cells cultured in vitro at high cell density (4 x 10(4) cells/cm2) on bacteriological petri dishes in the presence of serum pile up in clusters and create open spaces in the monolayer. This phenomenon is clearly visible 6 days after plating and is markedly enhanced by the addition of f...

journal_title：Experimental cell research

authors： De Wever B,Rooman RP,De Brabander M

更新日期：1990-01-01 00:00:00

abstract：:Mitotic PtK1 cells were treated at furrow initiation with microtubule poisons to determine the role of microtubules in the regulation of terminal cytokinetic events. The administration of anti-microtubule agents in late anaphase accelerated the rate of cytokinesis by approx. 60% as measured by changes in furrow diamet...

journal_title：Experimental cell research

authors： Hamilton BT,Snyder JA

更新日期：1983-04-01 00:00:00

abstract：:A novel methodology for labeling, isolation, and detection of nucleic acids is described. Nucleic acid isolation is based on in vivo or in vitro incorporation of BrU or BrdU to either RNA or DNA, respectively, followed by immunoprecipitation of the labeled nucleic acid utilizing anti-BrdU MoAb, which crossreacts with ...

journal_title：Experimental cell research

authors： Haider SR,Juan G,Traganos F,Darzynkiewicz Z

更新日期：1997-08-01 00:00:00

abstract：:Sertoli cell lines have been established from H-2K(b)-tsA58 transgenic mice carrying an inducible temperature-sensitive SV40 T antigen in their germline. All cell lines tested for expression of Sertoli cell products by reverse transcription-polymerase chain reaction were shown to express mRNAs for alpha-inhibin, Steel...

journal_title：Experimental cell research

authors： Walther N,Jansen M,Ergün S,Kascheike B,Ivell R

更新日期：1996-06-15 00:00:00

abstract：:Human T-cell leukemia/lymphotropic virus type I (HTLV-I) infection causes a variety of human diseases, including adult T-cell leukemia/lymphoma. The viral transactivator Tax has been implicated as a key factor in the HTLV-I-induced transformation pathway. To investigate the components of this pathway, we derived fibro...

journal_title：Experimental cell research

authors： Saggioro D,D'agostino DM,Chieco-Bianchi L

更新日期：1999-03-15 00:00:00

abstract：:Reversible methylation of the protein phosphatase 2A catalytic subunit (PP2A(C)(1)) is an important regulatory mechanism playing a crucial role in the selective recruitment of regulatory B subunits. Here, we investigated the subcellular localization of leucine carboxyl methyltransferase (LCMT1) and protein phosphatase...

journal_title：Experimental cell research

authors： Longin S,Zwaenepoel K,Martens E,Louis JV,Rondelez E,Goris J,Janssens V

更新日期：2008-01-01 00:00:00

abstract：:Telomere shortening in human liver with aging and chronic inflammation was examined by hybridization protection assay using telomere and Alu probes. The reduction rate of telomere repeats in normal liver (23 samples from patients 17-81 years old) was 120 bp per year, which is in good agreement with the reported reduct...

journal_title：Experimental cell research

authors： Aikata H,Takaishi H,Kawakami Y,Takahashi S,Kitamoto M,Nakanishi T,Nakamura Y,Shimamoto F,Kajiyama G,Ide T

更新日期：2000-05-01 00:00:00

abstract：:Natural BH3-memitic (-)-gossypol shows promising antitumor efficacy in several kinds of cancer. However, our previous studies have demonstrated that protective autophagy decreases the drug sensitivities of Bcl-2 inhibitors in hepatocellular carcinoma (HCC) cells. In the present study, we are the first to report that H...

journal_title：Experimental cell research

authors： Wang B,Chen L,Ni Z,Dai X,Qin L,Wu Y,Li X,Xu L,Lian J,He F

更新日期：2014-11-01 00:00:00

abstract：:We have investigated the effects of the lysosomotropic amines, ammonium chloride and chloroquine, on the delivery of fluid-phase pinocytic tracers to lysosomes in Chinese hamster ovary (CHO) cells. In preliminary experiments, 15 mM ammonium chloride and 0.1 mM chloroquine were found to be sufficient to give maximal pr...

journal_title：Experimental cell research

authors： Fritsch JE,Buckmaster MJ,Storrie B

更新日期：1988-04-01 00:00:00

abstract：:The protein encoded by C-terminal alternatively spliced p53 mRNA (p53as) has been shown previously to occur naturally in mouse cells and to bind sequence-specifically to DNA more efficiently than p53 (p53r, regular form). In the current study, p53as and p53r proteins ectopically expressed in p53-deficient cells each t...

journal_title：Experimental cell research

authors： Huang H,Kaku S,Knights C,Park B,Clifford J,Kulesz-Martin M

更新日期：2002-10-01 00:00:00

abstract：:The cellular targets for the Kirsten murine sarcoma virus (KiMSV)-transforming protein, p21ras, are unknown. Other studies have indicated that the mature form of p21 is distributed diffusely on the cytoplasmic face of the plasma membrane. However, after fixation without buffer washes, indirect immunofluorescent staini...

journal_title：Experimental cell research

authors： Myrdal SE,Auersperg N

更新日期：1985-08-01 00:00:00

abstract：:Laminins assemble into trimers composed of alpha, beta, and gamma chains which posttranslationally are glycosylated and sometimes proteolytically cleaved. In the current paper we set out to characterize posttranslational modifications and the laminin isoforms formed by laminin alpha1 and alpha5 chains. Comparative pul...

journal_title：Experimental cell research

authors： Champliaud MF,Virtanen I,Tiger CF,Korhonen M,Burgeson R,Gullberg D

更新日期：2000-09-15 00:00:00

abstract：:Cytotoxic T lymphocyte (CTL)-mediated lysis is accompanied by fragmentation of target cell DNA into an oligonucleosome ladder, a hallmark of apoptosis. Is this a fortuitous coincidence, or could CTL be inducing lysis by activation of the suicide signal? In this report we demonstrate that CTL-mediated target cell death...

journal_title：Experimental cell research

authors： Helgason CD,Shi L,Greenberg AH,Shi Y,Bromley P,Cotter TG,Green DR,Bleackley RC

更新日期：1993-06-01 00:00:00

abstract：:Regions of heterochromatin are often found at the periphery of the mammalian nucleus, juxtaposed to the nuclear lamina. Genes in these regions are likely maintained in a transcriptionally silent state, although other locations at the nuclear periphery associated with nuclear pores are sites of active transcription. As...

journal_title：Experimental cell research

authors： Lee DC,Welton KL,Smith ED,Kennedy BK

更新日期：2009-04-01 00:00:00

abstract：:A combination of broadly expressed transcriptional activators and spatially restricted repressors are used to pattern embryos into cells of different fate. Transcriptional co-regulators are essential mediators of transcription factor function, and contribute to selective transcriptional responses in embryo development...

journal_title：Experimental cell research

authors： Mannervik M

更新日期：2014-02-01 00:00:00

abstract：:Defects in the regulation of programmed cell death play a fundamental role in the development of neoplasia and neurological disorders, both of which are linked to the human T-cell leukemia/lymphoma virus type 1 (HTLV-1) infection. We previously showed that the HTLV-1 Tax protein protects from apoptosis induced by seru...

journal_title：Experimental cell research

authors： Trevisan R,Daprai L,Paloschi L,Vajente N,Chieco-Bianchi L,Saggioro D

更新日期：2006-05-01 00:00:00

abstract：:Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor which controls the expression of various genes involved in immune responses. However, it is not clear whether NF-κB activation is critical for phagocytosis when Staphylococcus aureus is the pathogen. Using oligonucleotide microarrays, we investigated ...

journal_title：Experimental cell research

authors： Zhu F,Yue W,Wang Y

更新日期：2014-10-01 00:00:00

abstract：:Recently, we isolated 4 distinct kinds of single chain antibody against human EGF receptor (EGFR) after screening the Keio phage display scFv library by using two methods of target-guided proximity labeling. In the current study, these monovalent scFv antibodies were converted to bivalent IgGs of humanized forms (hIgG...

journal_title：Experimental cell research

authors： Chang C,Takayanagi A,Yoshida T,Shimizu N

更新日期：2013-05-01 00:00:00

abstract：:To determine which endocytic compartments are sensitive to sucrose-induced osmotic swelling, CHO and Vero cells were cultured for 1-3 days in media containing 0.03 to 0.05 M sucrose. (Sucrose is internalized but not digested by these cells.) To immunolocalize late endocytic compartments, cells were fixed with formalde...

journal_title：Experimental cell research

authors： DeCourcy K,Storrie B

更新日期：1991-01-01 00:00:00

abstract：:The abundant heterogeneous nuclear ribonucleoprotein M (hnRNP M) is able to associate with early spliceosomes and to influence splicing patterns of specific pre-mRNAs. Here, by a combination of immunoprecipitation and pull-down assays, we have identified PSF (polypyrimidine tract-binding protein-associated splicing fa...

journal_title：Experimental cell research

authors： Marko M,Leichter M,Patrinou-Georgoula M,Guialis A

更新日期：2010-02-01 00:00:00

abstract：:Carbohydrate-specific monoclonal antibodies were used to demonstrate the expression of a new membrane glycoprotein on F9 murine embryonal carcinoma cells. Sialyl LeX was detected using monoclonal antibody FH6 in a sensitive, cell monolayer radioimmunoassay. The antigen codistributed in gel filtration of a crude homoge...

journal_title：Experimental cell research

authors： Rosenman SJ,Fenderson BA,Hakomori SI

更新日期：1989-02-01 00:00:00

abstract：:Carbonic anhydrase II (CA II) expression in characteristic for the early stage of osteoclast differentiation. To study how CA II, which is crucial in proton generation in mature osteoclasts, influences the osteoclast differentiation process we performed rat bone marrow cultures. In this model, acetazolamide, a specifi...

journal_title：Experimental cell research

authors： Lehenkari P,Hentunen TA,Laitala-Leinonen T,Tuukkanen J,Väänänen HK

更新日期：1998-07-10 00:00:00

abstract：:Screening for microbial metabolites that induce transcriptional activation of the SV40 promoter resulted in the identification of two known compounds, FR901228 and trichostatin A (TSA). FR901228 is a potent antitumor drug that is currently under clinical investigation. TSA is a specific inhibitor of histone deacetylas...

journal_title：Experimental cell research

authors： Nakajima H,Kim YB,Terano H,Yoshida M,Horinouchi S

更新日期：1998-05-25 00:00:00

abstract：BACKGROUND:CDK8 is associated with the transcriptional Mediator complex and has been shown to regulate several transcription factors implicated in cancer. As a pancreatic cancer oncogene, the role of CDK8 in cancer angiogenesis remains unclear. Here, we investigated the contribution of CDK8 in pancreatic cancer angioge...

journal_title：Experimental cell research

authors： Wei R,Kong L,Xiao Y,Yuan H,Song Y,Wang J,Yu H,Mao S,Xu W

更新日期：2018-08-15 00:00:00

abstract：:The formation of functional gap junctions between astrocytes was investigated during differentiation of these cells in culture. Precursor cells of GFA (glial fibrillary acidic) protein-positive astrocytes were cultured in a chemically defined medium as a homogeneous population. These cells were rarely coupled to one n...

journal_title：Experimental cell research

authors： Fischer G,Kettenmann H

更新日期：1985-08-01 00:00:00

abstract：:We found that a transient rise in cGMP levels, which was closely associated with the Ca2+ influx, occurred concomitant with the onset of myoblast fusion. The Ca2+ channel blocker D600 decreased both the cell fusion and the normal rise in cGMP levels. In contrast, the Ca2+ ionophore A23187 transiently increased cGMP le...

journal_title：Experimental cell research

authors： Choi SW,Baek MY,Kang MS

更新日期：1992-03-01 00:00:00

abstract：:12-Lipoxygenase, an arachidonic acid metabolizing enzyme of the lipoxygenase pathway, has been implicated as a major factor in promoting prostate cancer progression and metastasis. The ability of 12-LOX to aggravate the disease was linked to its proangiogenic role. Recent studies clearly demonstrated that 12-LOX enhan...

journal_title：Experimental cell research

authors： Krishnamoorthy S,Jin R,Cai Y,Maddipati KR,Nie D,Pagès G,Tucker SC,Honn KV

更新日期：2010-06-10 00:00:00

abstract：:Adhesion of hepatocytes to collagenous substrates and their spreading have been shown to involve a specific recognition event, possibly mediated by membrane proteins with affinity for collagen. In the present communication, we describe the isolation of membrane components that are involved in the adhesion of rat hepat...

journal_title：Experimental cell research

authors： Rubin K,Gullberg D,Borg TK,Obrink B

更新日期：1986-05-01 00:00:00

abstract：:Ultraviolet (UV) irradiation of cultured human skin fibroblasts causes the disassembly of their microtubules. Using indirect immunofluorescence microscopy, we have now investigated whether damage to the microtubule precursor pool may contribute to the disruption of microtubules. Exposure to polychromatic UV radiation ...

journal_title：Experimental cell research

authors： Zamansky GB,Perrino BA,Chou IN

更新日期：1991-07-01 00:00:00