Telomere reduction in human liver tissues with age and chronic inflammation.

abstract：:The rapidity of the optional 90-min differentiation of Naegleria gruberi from amoebae to flagellates suggests the possibility of a free-running cascade of events from initiating stimulus through gene expression to organelle assembly and cell morphogenesis. Instead our experiments reveal two points early in the differe...

journal_title：Experimental cell research

authors： Fulton C,Lai EY

更新日期：1998-08-01 00:00:00

abstract：:The p70 ribosomal S6 kinase (S6K1) is rapidly activated following growth factor stimulation of quiescent fibroblasts and inhibition of this enzyme results in a G(1) arrest. Phosphorylation of the ribosomal S6 protein by S6K1 regulates the translation of both ribosomal proteins and initiation factors, leading to an inc...

journal_title：Experimental cell research

authors： Zhang H,Hoff H,Marinucci T,Cristofalo VJ,Sell C

更新日期：2000-08-25 00:00:00

abstract：:This study reports on the specific binding of [3H]heparin to human adrenocortical carcinoma cell line SW-13. Heparin binding to SW-13 cells is specific, saturable, and time- and temperature-dependent with maximum binding occurring between 90 and 120 min at 22 degrees C. Scatchard analysis revealed two classes of bindi...

journal_title：Experimental cell research

authors： Halper J

更新日期：1990-04-01 00:00:00

abstract：:Fish keratocytes are an established model in single cell motility but little is known about their collective migration. Initially, sheets migrate from the scale at ~145 μm/h but over the course of 24h the rate of leading edge advance decreases to ~23 μm/h. During this period, leader cells retain their ability to migra...

journal_title：Experimental cell research

authors： Rapanan JL,Cooper KE,Leyva KJ,Hull EE

更新日期：2014-08-01 00:00:00

abstract：:Neuromedin B (NMB) is one of the bombesin-like peptides in mammals. Recently, bombesin-like peptides have been characterized as growth factors in highly vascularized tumors. In this study, we report that NMB potently stimulates in vivo neovascularization in a mouse Matrigel plug and the sprouting of endothelial cells ...

journal_title：Experimental cell research

authors： Park HJ,Kim SR,Bae SK,Choi YK,Bae YH,Kim EC,Kim WJ,Jang HO,Yun I,Kim YM,Bae MK

更新日期：2009-11-15 00:00:00

abstract：:A monoclonal antibody that reacts specifically with the proliferation-sensitive nuclear proteins, isoelectric focusing (IEF) 8Z30 and 8Z31 (molecular weight (MW), 76,000 charge variants, HeLa protein catalogue number) has been characterized. As determined by indirect immunofluorescence, the antibody stains the nucleol...

journal_title：Experimental cell research

authors： Celis JE,Madsen P,Nielsen S,Petersen Ratz G,Lauridsen JB,Celis A

更新日期：1987-02-01 00:00:00

abstract：:The interphase nucleus is a structurally ordered, three-dimensional structure, in which specific chromatin domains occupy distinct spatial positions that can, in turn, be modified with changes in cell function. A fundamental goal in developmental neurobiology is the identification of mechanisms that dictate the orderl...

journal_title：Experimental cell research

authors： Martou G,De Boni U

更新日期：2000-04-10 00:00:00

abstract：:We have tested whether life-long caloric restriction (CR) slows or delays the age-related loss of cellular replicative potential that occurs during normal aging in ad libitum (AL) fed mice. Both mean and maximum life spans of the restricted animals (60% of AL intake) were significantly extended 30-40% by CR treatment....

journal_title：Experimental cell research

authors： Pendergrass WR,Li Y,Jiang D,Fei RG,Wolf NS

更新日期：1995-04-01 00:00:00

abstract：:In this report, we elucidate the role of Na(+)-K+ pump in the regulation of polyamine spermidine (Spd) transport in murine leukemia (L 1210) cells in culture. Ouabain, known to bind extracellularly to the alpha-subunit of the Na(+)-K+ pump, inhibits the pump activity. The L 1210 cells were found to possess ouabain bin...

journal_title：Experimental cell research

authors： Khan NA,Quemener V,Moulinoux JP

更新日期：1992-04-01 00:00:00

abstract：:Peripheral neuroblastic tumors exist as a heterogeneous mixture of neuroblastic (N-type) cells and Schwannian stromal (S-type) cells. These stromal cells not only represent a differentiated and less aggressive fraction of the tumor, but also have properties that can influence the further differentiation of nearby mali...

journal_title：Experimental cell research

authors： Rogers DA,Schor NF

更新日期：2013-03-10 00:00:00

abstract：:Cytotoxic T lymphocyte (CTL)-mediated lysis is accompanied by fragmentation of target cell DNA into an oligonucleosome ladder, a hallmark of apoptosis. Is this a fortuitous coincidence, or could CTL be inducing lysis by activation of the suicide signal? In this report we demonstrate that CTL-mediated target cell death...

journal_title：Experimental cell research

authors： Helgason CD,Shi L,Greenberg AH,Shi Y,Bromley P,Cotter TG,Green DR,Bleackley RC

更新日期：1993-06-01 00:00:00

abstract：:Breast cancer and prostate cancer are the most common malignant tumors in female and men, respectively. Furthermore, lung cancer is the leading cause of cancer deaths worldwide. It is an emergency to develop a powerful strategy to treat these threatening cancers more effectively, because of low efficacy and high rates...

journal_title：Experimental cell research

authors： Karbasforooshan H,Roohbakhsh A,Karimi G

更新日期：2018-06-01 00:00:00

abstract：:A disintegrin and metalloproteinase 33 (ADAM33) has been identified as a susceptibility gene for asthma, but details of the causality are not fully understood. We hypothesize that soluble ADAM33 (sADAM33) overexpression can alter the mechanical behaviors of airway smooth muscle cells (ASMCs) via regulation of the cell...

journal_title：Experimental cell research

authors： Duan Y,Long J,Chen J,Jiang X,Zhu J,Jin Y,Lin F,Zhong J,Xu R,Mao L,Deng L

更新日期：2016-11-15 00:00:00

abstract：:14-3-3 proteins are a family of homologous eukaryotic molecules with seven distinct isoforms in mammalian cells. Isoforms of 14-3-3 proteins interact with diverse ligands and are involved in the regulation of mitogenesis, cell cycle progression, and apoptosis. However, whether different 14-3-3 isoforms are responsible...

journal_title：Experimental cell research

authors： Subramanian RR,Masters SC,Zhang H,Fu H

更新日期：2001-11-15 00:00:00

abstract：:Coiled bodies (CBs) are structural constituents observed in nuclei of most eukaryotic cells. They usually occur in the nucleoplasm as well as in contact with the nucleolar surface. In this work we studied the hepatocyte nuclei of hibernating dormice in order to investigate possible modifications of CBs along the seaso...

journal_title：Experimental cell research

authors： Malatesta M,Zancanaro C,Martin TE,Chan EK,Amalric F,Lührmann R,Vogel P,Fakan S

更新日期：1994-04-01 00:00:00

abstract：:Oxysterol-binding protein (OSBP)-related proteins (ORPs) are conserved lipid binding proteins found in organisms ranging from yeast to mammals. Recent findings have indicated that these proteins mainly localize to contact sites of 2 different membranous organelles. ORP6, a member of the ORP subfamily III, is one of th...

journal_title：Experimental cell research

authors： Mochizuki S,Miki H,Zhou R,Kido Y,Nishimura W,Kikuchi M,Noda Y

更新日期：2018-09-15 00:00:00

abstract：:Most developing lymphocytes spontaneously die in the thymus during positive and negative selection of the T cell repertoire. By evaluating the expression of the proliferation antigens Ki-67 and PCNA, we demonstrated here that more than 95% of thymocytes are potentially proliferating. The coincidence within the same ce...

journal_title：Experimental cell research

authors： Pellicciari C,Bottone MG,Schaack V,Barni S,Manfredi AA

更新日期：1996-12-15 00:00:00

abstract：:PDGF acts as an autocrine and paracrine factor in certain tumors through upregulation of the PDGF beta-receptor expression. In order to elucidate the control mechanism for the receptor expression, we have isolated an enhancer from two P1 clones that together contain a 102 kb NotI region covering the entire human PDGFR...

journal_title：Experimental cell research

authors： Kaneko M,Yang W,Matsumoto Y,Watt F,Funa K

更新日期：2006-07-01 00:00:00

abstract：:An epithelial intestinal cell line has been established from explants of fetal rat small intestine. After the 9th passage (approx. 25 population doublings) epithelial-like cells acquired the properties of a permanent cell line. The epithelial nature of this cell line, and of clone IRD 98 subsequently isolated, is supp...

journal_title：Experimental cell research

authors： Négrel R,Rampal P,Nano JL,Cavenel C,Ailhaud G

更新日期：1983-02-01 00:00:00

abstract：:SV40-transformation as well as treatment with tumor promoters produce alterations in morphology, differentiation and keratinization of human keratinocytes. Two cell lines of SV40-transformed keratinocytes and primary cultures of keratinocytes treated with the tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA) ...

journal_title：Experimental cell research

authors： Darmon M,Delescluse C,Semat A,Bernard B,Bailly J,Prunieras M

更新日期：1984-09-01 00:00:00

abstract：:The microtubule cytoskeleton is known to play a role in cell structure and serve as a scaffold for a variety of active molecules in processes as diverse as motility and cell division. The literature on the role of microtubules in signal transduction, however, is marked by inconsistencies. We have investigated a well-s...

journal_title：Experimental cell research

authors： Jackman RW,Rhoads MG,Cornwell E,Kandarian SC

更新日期：2009-11-15 00:00:00

abstract：:We made use of the 9-(1'-pyrene)nonanol/ultraviolet (P9OH/UV) method and isolated peroxisome-deficient mutant cells. TKa cells, the wild-type Chinese hamster ovary (CHO) cells, CHO-K1, that had been stably transfected with cDNA encoding Pex2p (formerly peroxisome assembly factor-1, PAF-1) were used to avoid frequent i...

journal_title：Experimental cell research

authors： Okumoto K,Bogaki A,Tateishi K,Tsukamoto T,Osumi T,Shimozawa N,Suzuki Y,Orii T,Fujiki Y

更新日期：1997-05-25 00:00:00

abstract：:DNA damage caused by alkylating agents results in a G2 checkpoint arrest. DNA mismatch repair (MMR) deficient cells are resistant to killing by alkylating agents and are unable to arrest the cell cycle in G2 phase after alkylation damage. We investigated the response of two MMR-deficient prostate cancer cell lines DU1...

journal_title：Experimental cell research

authors： Rasmussen LJ,Rasmussen M,Lützen A,Bisgaard HC,Singh KK

更新日期：2000-05-25 00:00:00

abstract：:In the naturally synchronous mitosis of the syncytial plasmodium of Physarum polycephalum, the nucleolus disintegrates in prophase, releasing a large amount of ribosomal RNA. Using biotinylated rDNA probes, we studied by high-resolution in situ hybridization the behavior of this nucleolar RNA throughout mitosis. Our r...

journal_title：Experimental cell research

authors： Pierron G,Puvion-Dutilleul F

更新日期：1993-10-01 00:00:00

abstract：:The macrophages have different subtypes with different functions in immune response and disease. It has been generally accepted that M1 macrophages are responsible for stimulation of immune system and inflammation while M2 macrophages play a role in tissue repair. Irrespective of the type, macrophage functions depend ...

journal_title：Experimental cell research

authors： Liu Y,Minze LJ,Mumma L,Li XC,Ghobrial RM,Kloc M

更新日期：2016-02-15 00:00:00

abstract：:Dystrophin is a cytoskeletal protein normally expressed underneath the sarcolemma of muscle fibers. The lack of dystrophin in Duchenne muscular Dystrophy (DMD) muscles results in fiber necrosis, which was proposed to be mediated by chronic calcium mishandling. The extensive comparison of dystrophic cells from human or...

journal_title：Experimental cell research

authors： Marchand E,Constantin B,Balghi H,Claudepierre MC,Cantereau A,Magaud C,Mouzou A,Raymond G,Braun S,Cognard C

更新日期：2004-07-15 00:00:00

abstract：:A polypeptide growth factor has been isolated from serum-free medium conditioned by mouse PC13 embryonal carcinoma cells, which is strongly mitogenic for Swiss 3T3 fibroblasts. On a CM-2-SW high-performance liquid chromatography cation-exchange column at low pH, this growth factor elutes at a salt concentration very c...

journal_title：Experimental cell research

authors： van Veggel JH,van Oostwaard TM,de Laat SW,van Zoelen EJ

更新日期：1987-04-01 00:00:00

abstract：:The enzyme endothelial Nitric Oxide Synthase (eNOS) is involved in key physiological and pathological processes, including cell motility and apoptosis. It is widely believed that at the cell surface eNOS is localized in caveolae, where caveolin-1 negatively regulates its activity, however, there are still uncertaintie...

journal_title：Experimental cell research

authors： Bulotta S,Cerullo A,Barsacchi R,De Palma C,Rotiroti D,Clementi E,Borgese N

更新日期：2006-04-01 00:00:00

abstract：:Breast cancer is the most common carcinoma that metastasizes to bone. Tumor-produced parathyroid hormone-related protein (PTHrP), a known stimulator of osteoclastic bone resorption, is a major mediator of the osteolytic process in breast cancer. We have previously shown that PTHrP increases breast cancer cell prolifer...

journal_title：Experimental cell research

authors： Shen X,Falzon M

更新日期：2006-11-15 00:00:00

abstract：:The biosynthesis of type XI and type II collagens was examined using a stable rat chondrocyte cell line established by W. E. Horton et al. (1988, Exp. Cell Res. 178, 457-468.). These cells (IRC; immortalized rat chondrocytes) were created by transformation with a murine retrovirus carrying the v-myc and v-raf oncogene...

journal_title：Experimental cell research

authors： Oxford JT,Doege KJ,Horton WE Jr,Morris NP

更新日期：1994-07-01 00:00:00