当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > Sertoli cell lines established from H-2Kb-tsA58 transgenic mice differentially regulate the expression of cell-specific genes.

Sertoli cell lines established from H-2Kb-tsA58 transgenic mice differentially regulate the expression of cell-specific genes.

Abstract：

:Sertoli cell lines have been established from H-2K(b)-tsA58 transgenic mice carrying an inducible temperature-sensitive SV40 T antigen in their germline. All cell lines tested for expression of Sertoli cell products by reverse transcription-polymerase chain reaction were shown to express mRNAs for alpha-inhibin, Steel factor, SGP-2, and transferrin as well as for androgen receptor and the orphan nuclear receptor SF-1. Selected cell lines were shown by immunocytochemistry to express the established Sertoli cell-specific pattern of cytoskeletal markers. The FSH receptor gene was also expressed, though downregulated by comparison with in vivo levels of expression. In some lines low expression of the luteinizing hormone receptor gene could also be detected. The gene for the transcription factor GATA-1, which is expressed specifically in Sertoli cells, was expressed only in a subset of the cell lines. Quantitative analysis of SGP-2 transcript levels by ribonuclease protection assays showed an increase at the nonpermissive temperature, whereas using a similar assay, Steel factor mRNA was shown to be expressed in amounts comparable to the in vivo situation only in two cell lines during permanent growth. In summary, cell lines that exhibit distinct Sertoli cell characteristics have been established, which may resemble different stages of phenotypic development.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Walther N,Jansen M,Ergün S,Kascheike B,Ivell R

doi

10.1006/excr.1996.0192

subject

Has Abstract

pub_date

1996-06-15 00:00:00

pages

411-21

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(96)90192-5

journal_volume

225

pub_type

杂志文章

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