Studies on mammalian chromosome replication. III. Organization and replication of diplochromosomes.

abstract：:Tendinopathy is a common and disabling condition that is difficult to treat. The pathomolecular events behind tendinopathy remain uncertain. Micro-RNAs (miRNAs, miRs) are short non-coding RNAs that regulate gene expression and may play a role in tendinopathy development. Tenocytes were obtained from human patellar ten...

journal_title：Experimental cell research

authors： Xiao M,Iglinski-Benjamin KC,Sharpe O,Robinson WH,Abrams GD

更新日期：2019-05-15 00:00:00

abstract：:Epidermal growth factor (EGF) has been shown to be a potent mitogen for epidermal cells both in vitro and in vivo, thus contributing to the development of an organism. It has recently become clear that peroxisome proliferator-activated receptor-beta/delta (PPARbeta/delta) expression and activation is involved in the c...

journal_title：Experimental cell research

authors： Liang P,Jiang B,Yang X,Xiao X,Huang X,Long J,Zhang P,Zhang M,Xiao M,Xie T,Huang X

更新日期：2008-10-15 00:00:00

abstract：:The intranuclear localization of SV40 T-antigen (T-Ag) and the cellular protein p53 was studied in SV40 abortively infected baby mouse kidney cells using two complementary methods of ultrastructural immunocytochemistry in combination with preferential staining of nuclear RNP components and electron microscope autoradi...

journal_title：Experimental cell research

authors： Puvion E,Duthu A,Harper F,Ehrhart JC,Viron A,May P

更新日期：1988-07-01 00:00:00

abstract：:A heterogeneous RNP structure has been isolated from rat liver nuclei by a method previously used for the isolation of 30S RNP complexes carrying heterogeneous RNA (hnRNA) [1]. The RNP sediments in sucrose gradients with s-values of 70-110S. Formaldehyde-fixed preparations band at Q = 1.40 in isopycnic CsCl gradients....

journal_title：Experimental cell research

authors： Hatzoglou M,Adamtziki E,Margaritis L,Sekeris CE

更新日期：1985-03-01 00:00:00

abstract：:The Saccharomyces cerevisiae ALG7 gene, which functions by initiating the dolichol pathway of protein N-glycosylation, displays properties of an early growth-response gene. To initiate studies of the involvement of ALG7 in cellular proliferation, we have now more precisely analyzed ALG7 expression in the G1 phase of c...

journal_title：Experimental cell research

authors： Pretel R,Lennon K,Bird A,Kukuruzinska MA

更新日期：1995-08-01 00:00:00

abstract：:Nucleotide sugar transporters play a central role in the process of glycosylation. They are responsible for the translocation of nucleotide sugars from the cytosol, their site of synthesis, into the Golgi apparatus where the activated sugars serve as substrates for a variety of glycosyltransferases. We and others have...

journal_title：Experimental cell research

authors： Lühn K,Laskowska A,Pielage J,Klämbt C,Ipe U,Vestweber D,Wild MK

更新日期：2004-12-10 00:00:00

abstract：:A novel fluorescence-based method was developed to measure HIV envelope glycoprotein (env)-CD4-mediated cell fusion. This method measures the spread of a fluorescent dye as the cytosolic compartments of adjacent cells become contiguous upon cell-to-cell fusion. Calcein-labeled CD4+ Sup-T1 cells were seeded onto a mono...

journal_title：Experimental cell research

authors： Pine PS,Weaver JL,Oravecz T,Pall M,Ussery M,Aszalos A

更新日期：1998-04-10 00:00:00

abstract：:Centromeres are the chromosomal loci that direct the formation of the kinetochores. These macromolecular assemblies mediate the interaction between chromosomes and spindle microtubules and thereby power chromosome movement during cell division. They are also the sites of extensive regulation of the chromosome segregat...

journal_title：Experimental cell research

authors： Bernad R,Sánchez P,Losada A

更新日期：2009-11-15 00:00:00

abstract：:Barrier-to-autointegration factor (BAF) is an essential chromatin protein conserved in metazoans. BAF has roles in nuclear assembly, chromatin organization, gene expression, and gonad development and is exploited by retroviruses. BAF forms stable dimers that bind nonspecifically to dsDNA and specifically to LEM-domain...

journal_title：Experimental cell research

authors： Tifft KE,Segura-Totten M,Lee KK,Wilson KL

更新日期：2006-02-15 00:00:00

abstract：:Tubules and vesicles are membrane carriers involved in traffic along the endocytic and secretory routes. The small GTPase Arf6 regulates a recycling branch of short dynamic tubular intermediates used by major histocompatibility class I (MHC-I) molecules to traffic through vesicles between endosomes and the plasma memb...

journal_title：Experimental cell research

authors： Massol RH,Larsen JE,Kirchhausen T

更新日期：2005-05-15 00:00:00

abstract：:The abilities of eight extracellular matrix proteins, fibronectin, vitronectin, laminin, and collagen types I, II, III, IV, and V to bind insulin were examined by binding studies with insulin conjugated with peroxidase. At a physiological pH and ionic strength, type V collagen bound to insulin most strongly. The other...

journal_title：Experimental cell research

authors： Yaoi Y,Hashimoto K,Takahara K,Kato I

更新日期：1991-06-01 00:00:00

abstract：:Our previous studies have showed that tumor-derived autophagosomes (termed "DRibbles") induce B cell activation, resulting in antibody production and cytokine secretion. Unexpectedly, we found that unfractionated splenocytes produced a higher level of antibody and cytokine than that of purified B cells. In the current...

journal_title：Experimental cell research

authors： Zhou M,Li W,Wen Z,Sheng Y,Ren H,Dong H,Cao M,Hu HM,Wang LX

更新日期：2015-02-15 00:00:00

abstract：:In the present study, we have tested the influence of inhibition of endocytosis by hypertonic medium on the regulation of cell surface insulin receptors. We show that active internalization of 125I-insulin is markedly inhibited by hypertonic media and that, in parallel, cell surface invaginations are significantly dim...

journal_title：Experimental cell research

authors： Geiger D,Carpentier JL,Gorden P,Orci L

更新日期：1989-11-01 00:00:00

abstract：:By fluorescence spectroscopy, the average pH within endocytic compartments was determined during endocytosis of fluorescein conjugates by macrophages and hepatocytes. In mouse macrophages and hepatocytes fluorescein conjugates taken up either in the fluid phase or by binding to cell surface receptors were rapidly tran...

journal_title：Experimental cell research

authors： Geisow MJ,Evans WH

更新日期：1984-01-01 00:00:00

abstract：:Myristoyl-CoA:protein N-myristoyltransferase (NMT) catalyzes the attachment of myristate onto the amino terminal glycine residue of select polypeptides. Cardiac tissue expresses high levels of cAMP-dependent protein kinase whose catalytic subunit is myristoylated; however, cardiac muscle extracts were found to contain...

journal_title：Experimental cell research

authors： Raju RV,Kakkar R,Datla RS,Radhi J,Sharma RK

更新日期：1998-05-25 00:00:00

abstract：:Mps-one-binder (Mob) proteins play a crucial role in yeast cytokinesis. After cloning two Mob1-like genes, MsMob1-A and MsMob1-B from alfalfa (Medicago sativa L.) we show that, although they are constitutively expressed in roots, stems, leaves, flowers and pods, their transcripts and proteins are mostly produced in ac...

journal_title：Experimental cell research

authors： Citterio S,Piatti S,Albertini E,Aina R,Varotto S,Barcaccia G

更新日期：2006-04-15 00:00:00

abstract：:The two major intermediate filament (IF) phosphoglycoproteins of human simple epithelia are keratins 8 and 18 (K8/18). Previous studies showed that heat stress and, in some cases, viral infection of cultured cells can be associated with alteration in IF organization. Here we show that heat stress of the human colonic ...

journal_title：Experimental cell research

authors： Liao J,Lowthert LA,Omary MB

更新日期：1995-08-01 00:00:00

abstract：:The involvement of polyamines in cytokinesis has been examined in eggs of the amphibian X. laevis. Microinjection of spermine or spermidine into unfertilized eggs induced a shortening of the first division cycle and early formation of cleavage-like constrictions. Eggs were activated by injection and developed furrows ...

journal_title：Experimental cell research

authors： Grant NJ,Aimar C,Oriol-Audit C

更新日期：1984-02-01 00:00:00

abstract：:Cisplatin resistance in cancer cells is due to a pleiotropic phenotype transition that allows cells to resist cell death. miRNAs have been shown to be reliable markers of phenotype, critical in cell differentiation, and dysregulated in cancer and other pathologies. Here we investigate the influence of miRNA on cisplat...

journal_title：Experimental cell research

authors： Pouliot LM,Shen DW,Suzuki T,Hall MD,Gottesman MM

更新日期：2013-02-15 00:00:00

abstract：OBJECTIVES:Vascular calcification is highly prevalent in patients with chronic kidney disease (CKD) and contributes to increased risk of cardiovascular disease and mortality. Accumulated evidences suggested that vascular smooth muscle cells (VSMCs) to osteoblast-like cells transdifferentiation (VOT) plays a crucial rol...

journal_title：Experimental cell research

authors： Wen P,Cao H,Fang L,Ye H,Zhou Y,Jiang L,Su W,Xu H,He W,Dai C,Yang J

更新日期：2014-04-01 00:00:00

abstract：:Normal and transformed human cells when stained for ezrin, an F-actin-binding ERM (ezrin/radixin/moesin) family protein, revealed a faint and intense immunofluorescence, respectively. Surprisingly, nuclear staining that was assigned to the nucleolus by confocal laser and immunoelectron microscopy was detected in both ...

journal_title：Experimental cell research

authors： Kaul SC,Kawai R,Nomura H,Mitsui Y,Reddel RR,Wadhwa R

更新日期：1999-07-10 00:00:00

abstract：:Pathological cardiac hypertrophy is a maladaptive response in a variety of organic heart disease (OHD), which is characterized by mitochondrial dysfunction that results from disturbed energy metabolism. SIRT3, a mitochondria-localized sirtuin, regulates global mitochondrial lysine acetylation and preserves mitochondri...

journal_title：Experimental cell research

authors： Yue Z,Ma Y,You J,Li Z,Ding Y,He P,Lu X,Jiang J,Chen S,Liu P

更新日期：2016-10-01 00:00:00

abstract：:We have compared the rate of synthesis, half-life, and steady-state content of the oncoprotein p53 in logarithmically growing cultures of (a) primary embryo, (b) immortalized but untransformed, and (c) spontaneously transformed mouse fibroblasts. Steady-state p53 content derived from metabolic labeling and immunopreci...

journal_title：Experimental cell research

authors： Williams NW,Wynford-Thomas D

更新日期：1989-10-01 00:00:00

abstract：:Bax-mediated apoptosis in neurons is involved in many pathologic conditions affecting the central nervous system, including degenerative diseases, stroke, and trauma. Two molecules belonging to the Bcl-2 family, Bcl-2 and Bcl-X(L), protect cells from Bax-induced apoptosis and show distinct expression patterns in adult...

journal_title：Experimental cell research

authors： Shinoura N,Satou R,Yoshida Y,Asai A,Kirino T,Hamada H

更新日期：2000-02-01 00:00:00

abstract：:Bovine papilloma virus-transformed hamster embryo fibroblasts (HEF-BPV) reacted to exposure to tricyclodecan-9-yl-xanthogenate (D609) with immediate reversion to the growth kinetics and the flat morphology of the untransformed parental cells. After six population doublings in the presence of D609, clones which display...

journal_title：Experimental cell research

authors： Amtmann E,Müller K,Knapp A,Sauer G

更新日期：1985-12-01 00:00:00

abstract：BACKGROUND:Functional activation of oncogenic K-Ras signaling pathway plays an important role in the early events of colorectal carcinogenesis (CRC). K-Ras proto-oncogene is involved in 35-40% of CRC cases. Mutations in the Ras gene trigger the transduction of proliferative and anti-apoptotic signals, even in the absen...

journal_title：Experimental cell research

authors： Naumov I,Kazanov D,Lisiansky V,Starr A,Aroch I,Shapira S,Kraus S,Arber N

更新日期：2012-01-15 00:00:00

abstract：:The expression patterns of the testis-specific and somatic forms of the pyruvate dehydrogenase (PDH) E1 alpha subunit genes were examined in adult mouse testis by in situ hybridization with specific cDNA probes and by immunostaining. A considerable increase in the mRNA level of the testis-specific PDH E1 alpha gene wa...

journal_title：Experimental cell research

authors： Takakubo F,Dahl HH

更新日期：1992-03-01 00:00:00

abstract：:Protein synthesis by Polyspondylium violaceum in response to the chemoattractant glorin was analyzed by two-dimensional gel electrophoresis to determine if glorin can affect gene expression. When cells developing in a shaking suspension culture were given glorin, five proteins exhibited significantly increased and thr...

journal_title：Experimental cell research

authors： Kopachik W

更新日期：1990-02-01 00:00:00

abstract：:The mechanisms of replicative senescence by telomere shortening are not fully understood. The Indian muntjac has the fewest chromosomes of all mammals, greatly simplifying the analysis of each telomere over time. In this study, telomere shortening was observed throughout the life span of cultured normal muntjac cells ...

journal_title：Experimental cell research

authors： Zou Y,Yi X,Wright WE,Shay JW

更新日期：2002-11-15 00:00:00

abstract：:Chondrocytes express CD44 as a primary receptor for the matrix macromolecule hyaluronan. Hyaluronan is responsible for the retention and organization of proteoglycan within cartilage, and hyaluronan-chondrocyte interactions are important for the assembly and maintenance of the cartilage matrix. Bovine articular chondr...

journal_title：Experimental cell research

authors： Aguiar DJ,Knudson W,Knudson CB

更新日期：1999-11-01 00:00:00