A collagen-binding protein involved in the differentiation of myoblasts recognizes the Arg-Gly-Asp sequence.


:We had earlier demonstrated that a 46-kDa glycoprotein is involved in the differentiation of rat skeletal myoblasts. We now show that the binding of this glycoprotein to collagen and gelatin is disrupted by Arg-Gly-Asp (RGD) containing peptide but not by Arg-Gly-Glu (RGE). The former peptide also selectively elutes the 46-kDa glycoprotein bound to gelatin-Sepharose. Since all other proteins which bind RGD sequences have been found at the cell surface, we attempted to localize the 46-kDa glycoprotein by means of immuno fluorescent staining and radioiodine labeling. Surprisingly, the majority of the protein was found to be localized in the endoplasmic reticulum. Protease treatment of a microsomal fraction revealed that the protein is in the interior of the reticulum. Immunoprecipitation experiments, using a polyclonal antibody against the 46-kDa protein, demonstrated that no closely related proteins exist in myoblasts and also confirmed that the protein was not a fragment of a cell-surface localized protein. These findings suggest that the RGD sequence is also used in protein recognition within the cell.


Exp Cell Res


Nandan D,Cates GA,Ball EH,Sanwal BD




Has Abstract


1988-11-01 00:00:00














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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Mathivanan I,Trepp C,Brunold C,Baerlocher G,Enzmann V

    更新日期:2015-04-10 00:00:00

  • Mitochondrial DNA is synthesized during meiosis and spermiogenesis in the mouse.

    abstract::Following intratesticular injection of [3H]thymidine, spermatozoa were isolated from the caput and cauda epididymis of CD-1 mice. Quantitation of the amount of radiolabelled DNA in whole spermatozoa, sperm heads (nuclei) and sperm midpiece-tails (mitochondria) at time intervals 3-36 days postinjection demonstrated mit...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hecht NB,Liem H

    更新日期:1984-09-01 00:00:00

  • Relationship of cytoskeletal filaments to annular gap junction expression in human adrenal cortical tumor cells in culture.

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Murray SA,Williams SY,Dillard CY,Narayanan SK,McCauley J

    更新日期:1997-08-01 00:00:00

  • The RGD integrin binding site in human L1-CAM is important for nuclear signaling.

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    journal_title:Experimental cell research

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    authors: Gast D,Riedle S,Kiefel H,Müerköster SS,Schäfer H,Schäfer MK,Altevogt P

    更新日期:2008-08-01 00:00:00

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    journal_title:Experimental cell research

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    authors: Puvion-Dutilleul F,Pierron G

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    journal_title:Experimental cell research

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    authors: Hobbs CA,Paul BA,Gilmour SK

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    journal_title:Experimental cell research

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    authors: Roggia C,Ukena C,Böhm M,Kilter H

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Imreh G,Hallberg E

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    journal_title:Experimental cell research

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    authors: Chen H,Liu Y,Hu Q

    更新日期:2015-11-01 00:00:00

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Misasi R,Garofalo T,Di Marzio L,Mattei V,Gizzi C,Hiraiwa M,Pavan A,Grazia Cifone M,Sorice M

    更新日期:2004-08-01 00:00:00

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    authors: McFarlane C,Hennebry A,Thomas M,Plummer E,Ling N,Sharma M,Kambadur R

    更新日期:2008-01-15 00:00:00

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ledermann B,Bürki K

    更新日期:1991-12-01 00:00:00

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Blose SH,Bushnell A

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    journal_title:Experimental cell research

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    authors: Enenstein J,Waleh NS,Kramer RH

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    authors: Korb T,Schlüter K,Enns A,Spiegel HU,Senninger N,Nicolson GL,Haier J

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    journal_title:Experimental cell research

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    authors: Choi SW,Baek MY,Kang MS

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    authors: Chow W,Hou G,Bendeck MP

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  • Tenascin-C in the extracellular matrix promotes the selection of highly proliferative and tubulogenesis-defective endothelial cells.

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: De Wever B,Rooman RP,De Brabander M

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    journal_title:Experimental cell research

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    journal_title:Experimental cell research

    pub_type: 杂志文章


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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Haider SR,Juan G,Traganos F,Darzynkiewicz Z

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  • Inhibition of protein dephosphorylation results in the accumulation of splicing snRNPs and coiled bodies within the nucleolus.

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lyon CE,Bohmann K,Sleeman J,Lamond AI

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Bell P,Scheer U

    更新日期:1999-04-10 00:00:00

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    journal_title:Experimental cell research

    pub_type: 杂志文章


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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Cuadrado A,Navarrete MH,Canovas JL

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  • Caspase-mediated apoptosis in AK-5 tumor cells: a cell-free study using peptide inhibitors and antisense strategy.

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Anjum R,Khar A

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Shin JH,Kim HW,Rhyu IJ,Kee SH

    更新日期:2016-01-01 00:00:00