Abstract:
:When exponentially growing NHIK 3025 cells were shifted from medium containing 30% serum to medium containing 0.03% serum the rate of net protein accumulation was reduced due to both a reduction in the rate of protein synthesis and an increase in the rate of protein degradation. This change in growth conditions increased the protein doubling time from 18 to 140 h. The cell cycle duration of cells synchronized by mitotic selection was, however, only increased from 17 to 26 h by this treatment. Therefore, when the cells divide by the end of the first cell cycle following synchronization, the cells shifted to 0.03% serum contained far less protein than those growing continuously in 30% serum. Hence, the attainment of a critical cell mass is probably not controlling cell division for cells growing in a balanced state.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Rønning OW,Pettersen EOdoi
10.1016/0014-4827(84)90788-2subject
Has Abstractpub_date
1984-11-01 00:00:00pages
267-72issue
1eissn
0014-4827issn
1090-2422pii
0014-4827(84)90788-2journal_volume
155pub_type
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