当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > Endothelial adhesion molecule ESAM binds directly to the multidomain adaptor MAGI-1 and recruits it to cell contacts.

Endothelial adhesion molecule ESAM binds directly to the multidomain adaptor MAGI-1 and recruits it to cell contacts.

Abstract：

:Endothelial cell-selective adhesion molecule (ESAM) is an immunoglobulin-like transmembrane protein associated with endothelial tight junctions (TJ). Based on a yeast two-hybrid screen, we have identified the membrane-associated guanylate kinase protein MAGI-1 as an intracellular binding partner of ESAM. MAGI-1 is a multidomain adaptor protein, which binds to transmembrane, cytoskeletal, and signaling molecules, and has been localized to tight junctions in epithelial cells. MAGI-1 associates with the very C-terminal sequence of ESAM most likely through a PDZ domain-mediated interaction. The direct interaction between ESAM and MAGI-1 was confirmed by pull-down experiments. The two proteins formed stable complexes in transfected Chinese hamster ovary (CHO) cells, which could be immunoisolated. We found MAGI-1 to be associated with cell-cell contacts in human umbilical vein endothelial cells (HUVECs) and in mouse endothelium, where it colocalizes with ESAM. In CHO cells, recruitment of MAGI-1 to cell contacts required the presence of ESAM. Hence, ESAM may be involved in anchoring MAGI-1 at endothelial tight junctions.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Wegmann F,Ebnet K,Du Pasquier L,Vestweber D,Butz S

doi

10.1016/j.yexcr.2004.07.010

keywords:

subject

Has Abstract

pub_date

2004-10-15 00:00:00

pages

121-33

issue

1

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(04)00390-8

journal_volume

300

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • The synthetic tubulysin derivative, tubugi-1, improves the innate immune response by macrophage polarization in addition to its direct cytotoxic effects in a murine melanoma model.

    abstract：:Synthetic tubugis are equally potent but more stable than their natural forms. Their anticancer potential was estimated on a solid melanoma in vitro and in vivo. Tubugi-1 induced the apoptosis in B16 cells accompanied with strong intracellular production of reactive species, subsequently imposing glutathione and thiol...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.04.028

    authors： Drača D,Mijatović S,Krajnović T,Pristov JB,Đukić T,Kaluđerović GN,Wessjohann LA,Maksimović-Ivanić D

    更新日期：2019-07-15 00:00:00

  • microRNA-181a downregulates deptor for TGFβ-induced glomerular mesangial cell hypertrophy and matrix protein expression.

    abstract：:TGFβ contributes to mesangial cell hypertrophy and matrix protein increase in various kidney diseases including diabetic nephropathy. Deptor is an mTOR-interacting protein and suppresses mTORC1 and mTORC2 activities. We have recently shown that TGFβ-induced inhibition of deptor increases the mTOR activity. The mechani...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.01.021

    authors： Maity S,Bera A,Ghosh-Choudhury N,Das F,Kasinath BS,Choudhury GG

    更新日期：2018-03-01 00:00:00

  • Protein kinase calpha is an effector of hexamethylene bisacetamide-induced differentiation of Friend erythroleukemia cells.

    abstract：:The program of biochemical and molecular events necessary for commitment to erythroid cell differentiation is particularly well characterized in murine Friend erythroleukemia cell lines. Commitment to hemoglobin synthesis in response to a variety of chemical inducers, including hexamethylene bisacetamide and dimethyl ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1998.4312

    authors： Mallia CM,Aguirre V,McGary E,Tang Y,Scandurro AB,Liu C,Noguchi CT,Beckman BS

    更新日期：1999-02-01 00:00:00

  • Antibodies specific to the plasma membrane of rat lung microvascular endothelium.

    abstract：:The highly purified, luminal domain of rat lung endothelial plasma membranes was used as an immunogen to obtain monoclonal antibodies to the endothelial cell surface. The procedure was highly efficient, yielding antibodies which recognize three seemingly novel endothelial integral membrane glycoproteins of molecular w...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3490

    authors： Ghitescu LD,Crine P,Jacobson BS

    更新日期：1997-04-10 00:00:00

  • Effects of Kaposi's sarcoma-associated herpesvirus ORF K1 on differentiating endothelium in murine embryoid bodies and teratomas.

    abstract：:Infection with Kaposi's sarcoma-associated herpesvirus (KSHV) is a prerequisite for Kaposi's sarcoma, a multicentric endothelial tumor of mixed blood vessel-lymphatic phenotype with a marked production of endothelial basement membrane proteins. The viral K1 gene encodes a unique transmembrane protein capable of transf...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2003.09.010

    authors： Mebrahtu S,Eriksson U,Rauch U,Warenholt J,Bendsöe N,Dictor M

    更新日期：2004-01-15 00:00:00

  • Functional and phenotypic characterization of two HL60 clones resistant to dimethylsulfoxide.

    abstract：:Two HL60 clones (C12 and C13) totally insensitive to differentiation induction by dimethylsulfoxide (Me2SO) are described. They have been growing continuously in the presence of the inducer for more than 6 months. The morphological and cytochemical features of the two populations are quite similar to those of the orig...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(83)90275-6

    authors： Ferrero D,Gallo E,Lanfrancone L,Tarella C

    更新日期：1983-08-01 00:00:00

  • Cell condensation in chondrogenic differentiation.

    abstract：:Reduction of intercellular spaces in the areas of prospective cartilage and bone formation (precartilage condensation) precedes chondrogenesis and may represent an important step in the process of cartilage differentiation during limb skeletogenesis. We have attempted to clarify the role of the microenvironment establ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/s0014-4827(05)80067-9

    authors： Tacchetti C,Tavella S,Dozin B,Quarto R,Robino G,Cancedda R

    更新日期：1992-05-01 00:00:00

  • The cytoplasmic domain of the alpha1 integrin subunit influences stress fiber formation via the conserved GFFKR motif.

    abstract：:Integrins are heterodimeric transmembrane proteins that mediate substrate adhesion and migration but also the bidirectional transfer of information across the plasma membrane via their cytoplasmic domains. We addressed the question of whether the very short cytoplasmic tail of the alpha1 integrin subunit of alpha1beta...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2000.4831

    authors： Vossmeyer D,Kaufmann C,Löster K,Lucka L,Horstkorte R,Reutter W,Danker K

    更新日期：2000-04-10 00:00:00

  • Knockdown of dual specificity phosphatase 4 enhances the chemosensitivity of MCF-7 and MCF-7/ADR breast cancer cells to doxorubicin.

    abstract：BACKGROUND:Breast cancer is the major cause of cancer-related deaths in females world-wide. Doxorubicin-based therapy has limited efficacy in breast cancer due to drug resistance, which has been shown to be associated with the epithelial-to-mesenchymal transition (EMT). However, the molecular mechanisms linking the EMT...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.08.023

    authors： Liu Y,Du F,Chen W,Yao M,Lv K,Fu P

    更新日期：2013-12-10 00:00:00

  • EAT/mcl-1 expression in the human embryonal carcinoma cells undergoing differentiation or apoptosis.

    abstract：:Differentiation and apoptosis are precisely regulated events in early embryogenesis. Retinoic acid-induced differentiation in the embryonal carcinoma (EC) cell line NCR-G3 triggers concurrent induction of apoptosis. Using this system, which serves as a model of early embryogenesis, the expression of various bcl-2-rela...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2001.5203

    authors： Sano M,Umezawa A,Abe H,Akatsuka A,Nonaka S,Shimizu H,Fukuma M,Hata J

    更新日期：2001-05-15 00:00:00

  • An improved method for the derivation of high quality iPSCs in the absence of c-Myc.

    abstract：:Induced pluripotent stem cells (iPSCs) hold tremendous potential for the development of new regenerative medicine therapies and the study of molecular mechanisms of pluripotency and development. However, reactivation of c-Myc, which results in tumor formation in chimeric mice, is a major roadblock in the translation o...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.09.014

    authors： Habib O,Habib G,Choi HW,Hong KS,Do JT,Moon SH,Chung HM

    更新日期：2013-12-10 00:00:00

  • Possible role of deep tubular invaginations of the plasma membrane in MHC-I trafficking.

    abstract：:Tubules and vesicles are membrane carriers involved in traffic along the endocytic and secretory routes. The small GTPase Arf6 regulates a recycling branch of short dynamic tubular intermediates used by major histocompatibility class I (MHC-I) molecules to traffic through vesicles between endosomes and the plasma memb...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2005.01.021

    authors： Massol RH,Larsen JE,Kirchhausen T

    更新日期：2005-05-15 00:00:00

  • Regulation of MEF2 transcriptional activity by calcineurin/mAKAP complexes.

    abstract：:The calcium/calmodulin-dependent protein phosphatase calcineurin is required for the induction of transcriptional events that initiate and promote myogenic differentiation. An important effector for calcineurin in striated muscle is the transcription factor myocyte enhancer factor 2 (MEF2). The targeting of the enzyme...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2012.12.016

    authors： Li J,Vargas MA,Kapiloff MS,Dodge-Kafka KL

    更新日期：2013-02-15 00:00:00

  • Dynamic reorganization of chemokine receptors, cholesterol, lipid rafts, and adhesion molecules to sites of CD4 engagement.

    abstract：:T cell polarization and redistribution of cellular components are critical to processes such as activation, migration, and potentially HIV infection. Here, we investigate the effects of CD4 engagement on the redistribution and localization of chemokine receptors, CXCR4 and CCR5, adhesion molecules, and lipid raft comp...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.11.022

    authors： Nguyen DH,Giri B,Collins G,Taub DD

    更新日期：2005-04-01 00:00:00

  • Scavenger receptors mediate adhesion of activated B lymphocytes.

    abstract：:The scavenger receptor (SR-A) is considered to play a role in host defense by scavenging endotoxins, oxidized proteins, and denatured or otherwise modified self components, which are routed toward degradation in macrophages. Recent data suggest that SR-A also functions as an adhesion molecule. Our previous finding of ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3876

    authors： Yokota T,Ehlin-Henriksson B,Hansson GK

    更新日期：1998-02-25 00:00:00

  • Identification and expression analysis of the zebrafish orthologues of the mammalian MAP1LC3 gene family.

    abstract：:Autophagy is the principle pathway within cells involved in clearing damaged proteins and organelles. Therefore autophagy is necessary to maintain the turnover balance of peptides and homoeostasis. Autophagy occurs at basal levels under normal conditions but can be upregulated by chemical inducers or stress conditions...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.07.014

    authors： Ganesan S,Moussavi Nik SH,Newman M,Lardelli M

    更新日期：2014-10-15 00:00:00

  • The Drosophila orthologue of xPlkk1 is not essential for Polo activation and is necessary for proper contractile ring formation.

    abstract：:Polo-like kinases (Plks) are essential for progression through mitosis. The activity of these kinases peak during M phase and this activation has been attributed to phosphorylation. Kinases capable of activating Plks in vitro have been previously identified both in mammalian cells and in Xenopus laevis oocytes (SLK an...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2005.10.033

    authors： Alves PS,Godinho SA,Tavares AA

    更新日期：2006-02-01 00:00:00

  • Studies on mammalian chromosome replication. III. Organization and replication of diplochromosomes.

    abstract：:The process of DNA synthesis in normal and endoreduplicating mammalian cells are very similar. Both types of chromosomes are replicated in defined units termed chromosomal replicons, and in the same sequences along their lengths. The sister chromosomes of the diplochromosomes are replicated synchronously in identical ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(83)90148-9

    authors： Lau YF

    更新日期：1983-07-01 00:00:00

  • Integrin alpha6 cleavage: a novel modification to modulate cell migration.

    abstract：:Integrins play a major role in cell adhesion and migration. Previous work reported that a cleaved form of integrin alpha6 (alpha6p) was detected in invasive human prostate cancer tissue, absent in normal prostate tissue and was produced by urokinase-type Plasminogen Activator (uPA) in a plasmin-independent manner. Usi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2007.01.006

    authors： Pawar SC,Demetriou MC,Nagle RB,Bowden GT,Cress AE

    更新日期：2007-04-01 00:00:00

  • Mevalonate is essential for growth activation of human fibroblasts: evidence for a critical role of protein glycosylation in the prereplicative period.

    abstract：:Human diploid fibroblasts, arrested following serum or mevalonate depletion, were restimulated to a maximal rate of DNA synthesis within 24 h after the addition of serum or mevalonate, respectively. In both cases the initiation of DNA synthesis was preceded by a 12-h prereplicative phase. Upon the stimulation with ser...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1994.1155

    authors： Carlberg M,Hjertman M,Wejde J,Larsson O

    更新日期：1994-06-01 00:00:00

  • Towards a further understanding of the growth-inhibiting action of oxygen deficiency. Evaluation of the effect of antimycin on proliferating Ehrlich ascites tumour cells.

    abstract：:The effect of 1 microM antimycin on the proliferative properties, metabolism and basic cell composition of Ehrlich ascites tumour cells cultured in the second in vitro passage was studied. Continuous drug exposure of asynchronous cells caused rapid cessation of cell growth, characterized by the cell number and DNA, RN...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90162-4

    authors： Löffler M

    更新日期：1985-03-01 00:00:00

  • A tubulin identified by a monoclonal antibody is not present in mitotic spindles.

    abstract：:A monoclonal antibody, G8, which recognizes a form of tubulin (G8-tubulin) with a novel distribution in Rat-1 cells and Potorous tridactylis kidney (Ptk-2) cells was isolated. G8 labeled the interphase cytoskeleton of Rat-1 fibroblasts but not mitotic spindles or midbodies. G8 also stained a fiber network in some but ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(89)90345-5

    authors： Gossels JM,Ingram VM

    更新日期：1989-10-01 00:00:00

  • TNF-alpha and 9-cis-retinoic acid synergistically induce ICAM-1 expression: evidence for interaction of retinoid receptors with NF-kappa B.

    abstract：:TNF-alpha and 9-cis-retinoic acid (9-cis-R) synergistically enhance ICAM-1 protein expression in immortalized human aortic endothelial cells (HAECTs). At a TNF-alpha concentration of 0.1 ng/ml, 1 microM 9-cis-R enhanced ICAM-1 protein expression 4-fold. Treatment with 1 microM 9-cis-R alone caused no induction of ICAM...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3913

    authors： Chadwick CC,Shaw LJ,Winneker RC

    更新日期：1998-03-15 00:00:00

  • Retinal differentiation of human bone marrow-derived stem cells by co-culture with retinal pigment epithelium in vitro.

    abstract：:The goal of this study was to assess the in vitro differentiation capacity of human bone marrow-derived stem cells (hBMSCs) along retinal lineages. Mononuclear cells (MNC) were isolated from bone marrow (BM) and mobilized peripheral blood (mPB) using Ficoll-Paque density gradient centrifugation, and were sorted by mag...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2015.02.001

    authors： Mathivanan I,Trepp C,Brunold C,Baerlocher G,Enzmann V

    更新日期：2015-04-10 00:00:00

  • The PCNA-associated factor KIAA0101/p15(PAF) binds the potential tumor suppressor product p33ING1b.

    abstract：:The KIAA0101/p15(PAF)/OEATC-1 protein was initially isolated in a yeast two-hybrid screen for proliferating cell nuclear antigen (PCNA) binding partners, and was shown to bind PCNA competitively with the cell cycle regulator p21(WAF). PCNA is involved in DNA replication and damage repair. Using polyclonal antisera rai...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2005.09.020

    authors： Simpson F,Lammerts van Bueren K,Butterfield N,Bennetts JS,Bowles J,Adolphe C,Simms LA,Young J,Walsh MD,Leggett B,Fowles LF,Wicking C

    更新日期：2006-01-01 00:00:00

  • Tfe3 expression is closely associated to macrophage terminal differentiation of human hematopoietic myeloid precursors.

    abstract：:The MItf-Tfe family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors encodes four family members: MItf, Tfe3, TfeB and TfeC. In vitro, each protein of the family binds DNA in a homo- or heterodimeric form with other family members. Tfe3 is involved in chromosomal translocations recurrent in di...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.09.015

    authors： Zanocco-Marani T,Vignudelli T,Gemelli C,Pirondi S,Testa A,Montanari M,Parenti S,Tenedini E,Grande A,Ferrari S

    更新日期：2006-12-10 00:00:00

  • A collagen-binding protein involved in the differentiation of myoblasts recognizes the Arg-Gly-Asp sequence.

    abstract：:We had earlier demonstrated that a 46-kDa glycoprotein is involved in the differentiation of rat skeletal myoblasts. We now show that the binding of this glycoprotein to collagen and gelatin is disrupted by Arg-Gly-Asp (RGD) containing peptide but not by Arg-Gly-Glu (RGE). The former peptide also selectively elutes th...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(88)90368-0

    authors： Nandan D,Cates GA,Ball EH,Sanwal BD

    更新日期：1988-11-01 00:00:00

  • Isoforms of p38MAPK gamma and delta contribute to differentiation of human AML cells induced by 1,25-dihydroxyvitamin D₃.

    abstract：:Inhibition of p38MAPK alpha/beta is known to enhance 1,25-dihydroxyvitamin (1,25D)-induced monocytic differentiation, but the detailed mechanism of this effect was not clear. We now show that the enhancement of differentiation becomes apparent with slow kinetics (12-24 h). Interestingly, the inhibition of p38MAPK alph...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2010.08.010

    authors： Zhang J,Harrison JS,Studzinski GP

    更新日期：2011-01-01 00:00:00

  • Mcl-1 antagonism is a potential therapeutic strategy in a subset of solid cancers.

    abstract：:Cancer cell survival is frequently dependent on the elevated levels of members of the Bcl-2 family of prosurvival proteins that bind to and inactivate BH3-domain pro-apoptotic cellular proteins. Small molecules that inhibit the protein-protein interactions between prosurvival and proapoptotic Bcl-2 family members (so-...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.11.022

    authors： Modugno M,Banfi P,Gasparri F,Borzilleri R,Carter P,Cornelius L,Gottardis M,Lee V,Mapelli C,Naglich JG,Tebben A,Vite G,Pastori W,Albanese C,Corti E,Ballinari D,Galvani A

    更新日期：2015-03-15 00:00:00

  • Migration-stimulating factor (MSF) is over-expressed in non-small cell lung cancer and promotes cell migration and invasion in A549 cells over-expressing MSF.

    abstract：:Migration-stimulating factor (MSF), an oncofetal truncated isoform of fibronectin, is a potent stimulator of cell invasion. However, its distribution and motogenic role in non-small cell lung cancer (NSCLC) have never been identified. In this study, real-time PCR and immunohistochemical staining (IHC) were performed t...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.05.016

    authors： Deng X,Ma Q,Zhang B,Jiang H,Zhang Z,Wang Y

    更新日期：2013-10-15 00:00:00