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TIEG1-null tenocytes display age-dependent differences in their gene expression, adhesion, spreading and proliferation properties.

Abstract：

:The remodeling of extracellular matrix is a crucial mechanism in tendon development and the proliferation of fibroblasts is a key factor in this process. The purpose of this study was to further elucidate the role of TIEG1 in mediating important tenocyte properties throughout the aging process. Wildtype and TIEG1 knockout tenocytes adhesion, spreading and proliferation were characterized on different substrates (fibronectin, collagen type I, gelatin and laminin) and the expression levels of various genes known to be involved with tendon development were analyzed by RT-PCR. The experiments revealed age-dependent and substrate-dependent properties for both wildtype and TIEG1 knockout tenocytes. Taken together, our results indicate an important role for TIEG1 in regulating tenocytes adhesion, spreading, and proliferation throughout the aging process. Understanding the basic mechanisms of TIEG1 in tenocytes may provide valuable information for treating multiple tendon disorders.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Haddad O,Gumez L,Hawse JR,Subramaniam M,Spelsberg TC,Bensamoun SF

doi

10.1016/j.yexcr.2011.05.007

subject

Has Abstract

pub_date

2011-07-15 00:00:00

pages

1726-35

issue

12

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(11)00172-8

journal_volume

317

pub_type

杂志文章

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