Variation and frequency of cytokeratin polypeptide patterns in human squamous non-keratinizing epithelium.

abstract：:We have isolated a clone of human lymphoblastoid cells that is capable of undergoing the phenomenon of contact-mediated cell spreading in vitro. We have detected this behavior when using both transmission electron microscopy (TEM), and differential interference contrast microscopy. Upon cell-cell contact, cells become...

journal_title：Experimental cell research

authors： Sorrentino JA,Millis AJ,Whipple AP,Colquhoun WR

更新日期：1983-05-01 00:00:00

abstract：:USF is a small family of basic helix-loop-helix leucine zipper (bHLH-zip) transcription factors with DNA binding specificities similar to that of the c-Myc oncoprotein. Evidence for a role of USF in growth control includes inhibition of c-Myc-dependent cellular transformation in vitro and loss of USF transcriptional a...

journal_title：Experimental cell research

authors： Choe C,Chen N,Sawadogo M

更新日期：2005-01-01 00:00:00

abstract：:Viral vectors hold promise and challenges in gene therapy. Specifically, we have previously shown that baculoviral (BV) vectors have a high efficiency of gene delivery in human embryonic stem (ES) cells. Here we report the development of a complementary system to further our evaluation by utilizing the laboratory fish...

journal_title：Experimental cell research

authors： Yan Y,Du J,Chen T,Yi M,Li M,Wang S,Li CM,Hong Y

更新日期：2009-08-01 00:00:00

abstract：:The correct functioning of epidermal growth factor receptor (EGFR), a receptor tyrosine kinase, is required for normal skin development and homeostasis. Cellular hyperproliferation induced by dysregulation of EGFR is tightly associated with structural and functional defects of hair follicles, skin lesions, and tumorig...

journal_title：Experimental cell research

authors： Chen L,Wang T,Ji X,Ding C,Liang T,Liu X,Lu J,Guo X,Kang Q,Ji Z

更新日期：2019-11-15 00:00:00

abstract：:There are a number of low-abundance small nuclear RNAs (snRNAs) in eukaryotic cells. Many of them have been assigned functions in the biogenesis of cellular RNAs, such as splicing and 3' end processing. Here, we present the sequence of Xenopus U12 snRNA and compare the secondary structures of the low-abundance U11 and...

journal_title：Experimental cell research

authors： Yu YT,Tarn WY,Yario TA,Steitz JA

更新日期：1996-12-15 00:00:00

abstract：:In human and non-human primates, migratory trophoblasts penetrate the uterine epithelium, invade the endometrium, enter the uterine vasculature, and migrate within the arteries. The mechanisms that regulate this directional migration are unknown. We have used early gestation macaque trophoblasts to test the hypothesis...

journal_title：Experimental cell research

authors： Thirkill TL,Lowe K,Vedagiri H,Blankenship TN,Barakat AI,Douglas GC

更新日期：2005-05-01 00:00:00

abstract：:Myristoyl-CoA:protein N-myristoyltransferase (NMT) catalyzes the attachment of myristate onto the amino terminal glycine residue of select polypeptides. Cardiac tissue expresses high levels of cAMP-dependent protein kinase whose catalytic subunit is myristoylated; however, cardiac muscle extracts were found to contain...

journal_title：Experimental cell research

authors： Raju RV,Kakkar R,Datla RS,Radhi J,Sharma RK

更新日期：1998-05-25 00:00:00

abstract：:We found that a transient rise in cGMP levels, which was closely associated with the Ca2+ influx, occurred concomitant with the onset of myoblast fusion. The Ca2+ channel blocker D600 decreased both the cell fusion and the normal rise in cGMP levels. In contrast, the Ca2+ ionophore A23187 transiently increased cGMP le...

journal_title：Experimental cell research

authors： Choi SW,Baek MY,Kang MS

更新日期：1992-03-01 00:00:00

abstract：:In the present study, we have tested the influence of inhibition of endocytosis by hypertonic medium on the regulation of cell surface insulin receptors. We show that active internalization of 125I-insulin is markedly inhibited by hypertonic media and that, in parallel, cell surface invaginations are significantly dim...

journal_title：Experimental cell research

authors： Geiger D,Carpentier JL,Gorden P,Orci L

更新日期：1989-11-01 00:00:00

abstract：:High glucose concentrations associated with diabetes have been shown to cause the nonenzymatic modification of proteins. Reducing sugars covalently bind to free amine groups, undergo Amadori rearrangements, and crosslink with other glucose-modified proteins. Crosslinking of type I collagen by incubation with different...

journal_title：Experimental cell research

authors： Howard EW,Benton R,Ahern-Moore J,Tomasek JJ

更新日期：1996-10-10 00:00:00

abstract：:Previously we showed that the potocytosis of 5-methyltetrahydrofolate is dependent on the acidification of plasmalemmal vesicles created each time a caveolae, closes off from the cell surface. We now report that bafilomycin A1, which is a specific inhibitor of the V-type proton ATPase, inhibits 5-methyltetrahydrofolat...

journal_title：Experimental cell research

authors： Mineo C,Anderson RG

更新日期：1996-05-01 00:00:00

abstract：:Microfilament cores, obtained by extracting 13762 mammary ascites tumor cell microvilli with Triton X-100, contain a major glycoprotein migrating at an apparent molecular weight of 80 kDa by dodecyl sulfate-polyacrylamide gel electrophoresis. The 80-kDa component is a disulfide-linked multimer, as demonstrated by velo...

journal_title：Experimental cell research

authors： Ye XH,Metcalf TN 3rd,Andrews DM,Carraway CA,Carraway KL

更新日期：1989-05-01 00:00:00

abstract：:Interference of colchicine with meiotic chromosome pairing in the wild garlic, Allium ursinum, was studied using a whole-mount spreading technique for synaptonemal complexes. Colchicine was found to cause (i) pairing suppression (arrest of leptotene) and (ii) deficient pairing initiation at zygotene in connection with...

journal_title：Experimental cell research

authors： Loidl J

更新日期：1988-09-01 00:00:00

abstract：:Using sequential extractions with buffers containing or not containing neutral detergent, two highly insoluble protein components were found in livers of 30-day-old rats. These compounds (molecular weights (MW) 52 900 and 33 200 respectively) were practically absent from livers of young rats (between 5 and 8 postnatal...

journal_title：Experimental cell research

authors： Zanetta JP,Bingen A,Dontenwill M,Vincendon G

更新日期：1984-12-01 00:00:00

abstract：:Laminins are structurally and functionally major components of the extracellular matrix. Four isoforms of laminins (laminin-1, -2, -5 and -10) are expressed in a specific pattern along the crypt-villus axis of the intestine. Previous works indicated that expression of these isoforms is developmentally regulated and th...

journal_title：Experimental cell research

authors： Turck N,Gross I,Gendry P,Stutzmann J,Freund JN,Kedinger M,Simon-Assmann P,Launay JF

更新日期：2005-02-15 00:00:00

abstract：:SIRT5 has a wide range of functions in different cellular processes such as glycolysis, TCA cycle and antioxidant defense, which mediates lysine desuccinylation, deglutarylation and demalonylation. Recent evidences have implicated that SIRT5 is a potential suppressor of gastric cancer (GC). However, the underlying mec...

journal_title：Experimental cell research

authors： Lu X,Yang P,Zhao X,Jiang M,Hu S,Ouyang Y,Zeng L,Wu J

更新日期：2019-09-15 00:00:00

abstract：:The altered patterns of expression of gangliosides during density-dependent growth inhibition, oncogenic transformation, and embryogenesis suggest that gangliosides, sialylated membrane glycolipids, may affect cellular proliferation and differentiation. Gangliosides of the "b" pathway of ganglioside synthesis, includi...

journal_title：Experimental cell research

authors： Paller AS,Arnsmeier SL,Fisher GJ,Yu QC

更新日期：1995-03-01 00:00:00

abstract：:Pevonedistat is a potent, selective, first-in-class NEDD8 activating enzyme inhibitor. It is now under multiple clinical trials that investigate its anticancer effect against solid tumors and leukemia. ATP-binding cassette (ABC) transporters are membrane proteins that are involved in mediating multidrug resistance (MD...

journal_title：Experimental cell research

authors： Wei LY,Wu ZX,Yang Y,Zhao M,Ma XY,Li JS,Yang DH,Chen ZS,Fan YF

更新日期：2020-03-15 00:00:00

abstract：:Phorbol dibutyrate (PDBu) induced the formation of podosome-like structures together with partial disassembly of actin stress fibers in A7r5 smooth muscle cells. These podosomes contained alpha-actinin, F-actin, and vinculin and exhibit a tubular, column-like structure arising perpendicularly from the bottom of PDBu-t...

journal_title：Experimental cell research

authors： Hai CM,Hahne P,Harrington EO,Gimona M

更新日期：2002-10-15 00:00:00

abstract：:Cytotoxic T lymphocyte (CTL)-mediated lysis is accompanied by fragmentation of target cell DNA into an oligonucleosome ladder, a hallmark of apoptosis. Is this a fortuitous coincidence, or could CTL be inducing lysis by activation of the suicide signal? In this report we demonstrate that CTL-mediated target cell death...

journal_title：Experimental cell research

authors： Helgason CD,Shi L,Greenberg AH,Shi Y,Bromley P,Cotter TG,Green DR,Bleackley RC

更新日期：1993-06-01 00:00:00

abstract：:The presence of about 1.2 M glycerol during transfection with DNA/transferrin-polylysine and DNA/polylysine complexes dramatically increases transgene expression in a variety of cell types, provided that the complexes have an excess of polylysine. We have characterized this phenomenon using a human melanoma cell line ...

journal_title：Experimental cell research

authors： Zauner W,Kichler A,Schmidt W,Mechtler K,Wagner E

更新日期：1997-04-10 00:00:00

abstract：:The progression of cancer cells to highly malignant phenotypes that ultimately invade and metastasize to near and distant sites requires that metastatic cells respond to mitogenic signals in new microenvironments. The successful growth of metastases is due to their ability to respond to local paracrine growth factors ...

journal_title：Experimental cell research

authors： Nicolson GL

更新日期：1993-02-01 00:00:00

abstract：:Two SV40-transformed human cell lines, GM637, derived from a normal human subject, and GM5849, derived from a patient with ataxia-telangiectasia (A-T), were grown in increasing concentrations of the cytotoxic agent methotrexate (MTX). The GM637 line was naturally more resistant to methotrexate than was GM5849 and, ove...

journal_title：Experimental cell research

authors： Hahn P,Kapp LN,Painter RB

更新日期：1987-01-01 00:00:00

abstract：:K252a, a protein kinase inhibitor, acts as a neurotrophic factor in several neuronal cells. In this study we show that K252a enhanced the differentiation of C2C12 myoblasts as well as tyrosine phosphorylation of several focal adhesion-associated proteins including p130(Cas), focal adhesion kinase, and paxillin. The ty...

journal_title：Experimental cell research

authors： Lee KH,Lee SH,Kim D,Rhee S,Kim C,Chung CH,Kwon H,Kang MS

更新日期：1999-11-01 00:00:00

abstract：:In most existing transgenic mouse models developed for the study of specific genes in the skin, the goal has been to target transgene expression to defined populations of cells in the cutaneous epithelium. Keratin promoters have been especially useful for this purpose. In some instances, however, it may be desirable t...

journal_title：Experimental cell research

authors： Sawicki JA,Morris RJ,Monks B,Sakai K,Miyazaki J

更新日期：1998-10-10 00:00:00

abstract：:Cell surface beta-1,4-galactosyltransferase (GalTase) partially mediates a variety of cell interactions with laminin-containing matrices, including mesenchymal cell spreading and migration and neurite initiation, by binding to N-linked oligosaccharides within the E8 domain of laminin. Previous studies using indirect i...

journal_title：Experimental cell research

authors： Eckstein DJ,Shur BD

更新日期：1992-07-01 00:00:00

abstract：:Germ cells were isolated from mouse fetal gonads 11 1/2-16 1/2 days post coitum (dpc), and exposed to various methods of in vitro culture. From 13 1/2 dpc onwards, both male and female germ cells survived well at 37 degrees C for several days. During the culture period the proportion of female germ cells in meiosis in...

journal_title：Experimental cell research

authors： De Felici M,McLaren A

更新日期：1983-04-01 00:00:00

abstract：:Epidermal growth factor (EGF) has been shown to be a potent mitogen for epidermal cells both in vitro and in vivo, thus contributing to the development of an organism. It has recently become clear that peroxisome proliferator-activated receptor-beta/delta (PPARbeta/delta) expression and activation is involved in the c...

journal_title：Experimental cell research

authors： Liang P,Jiang B,Yang X,Xiao X,Huang X,Long J,Zhang P,Zhang M,Xiao M,Xie T,Huang X

更新日期：2008-10-15 00:00:00

abstract：:The ability of primary cultures of rat hepatocytes to remove cyclobutane pyrimidine dimers (CPDs) from DNA fragments containing the transcriptionally active albumin gene and the transcriptionally inactive embryonic myosin heavy chain (MHCemb) and H-ras fragments as well as the genome overall was measured. At all UV do...

journal_title：Experimental cell research

authors： Guo Z,Heydari A,Richardson A

更新日期：1998-11-25 00:00:00

abstract：:A novel protein that associates with interphase nucleus and mitotic apparatus (INMAP) was identified by screening HeLa cDNA expression library with an autoimmune serum followed by tandem mass spectrometry. Its complete cDNA sequence of 1.818 kb encodes 343 amino acids with predicted molecular mass of 38.2 kDa and nume...

journal_title：Experimental cell research

authors： Shen E,Lei Y,Liu Q,Zheng Y,Song C,Marc J,Wang Y,Sun L,Liang Q

更新日期：2009-04-15 00:00:00