Abstract:
:Disintegrins, the snake venom-derived arginine-glycine-aspartic acid-containing peptides, have been demonstrated to inhibit angiogenesis through induction of endothelial cell apoptosis. However, it is not clear how a disintegrin causes endothelial apoptosis. In this study, we elucidated the action mechanism of disintegrin in causing endothelial apoptosis by using rhodostomin as a tool. We showed that cell detachment was observed at the early stage of rhodostomin treatment. It was initiated through the blockade by integrin alphanubeta3 and was accelerated by a mechanical stretch from neighboring cells. Both rhodostomin and poly(HEME) induced a higher percentage of cells at G2-M phase, the cleavage of beta-catenin and poly(ADP-ribose) polymerase during apoptosis, indicating that cell detachment is a prerequisite for rhodostomin-induced apoptosis. Moreover, pp125(FAK) phosphorylation and actin cytoskeleton were affected upon rhodostomin treatment. The activation of caspase-3 but not that of caspase-9 was detected after rhodostomin treatment. In addition, general caspase inhibitors inhibited the cleavage of beta-catenin and poly(ADP-ribose) polymerase, and DNA fragmentation, whereas they did not prevent cell shape change or detachment. According to these results, we concluded that disintegrin-induced endothelial apoptosis is a complex process, not merely caused by a blockade of endothelial integrin alphanubeta3 but also by an accompanied shape change and mechanical stretches among cells.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Wu WB,Peng HC,Huang TFdoi
10.1016/s0014-4827(03)00105-8keywords:
subject
Has Abstractpub_date
2003-05-15 00:00:00pages
115-27issue
1eissn
0014-4827issn
1090-2422pii
S0014482703001058journal_volume
286pub_type
杂志文章abstract::Rabbit smooth muscle cells (SMC) in primary culture attached to and started proliferating on native and heat-denatured type I collagens, although the amount of cell attachment to denatured collagen was significantly lower. The cells adhered poorly and were unable to grow on commercial gelatin. In contrast, synthetic S...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1995.1225
更新日期:1995-07-01 00:00:00
abstract::We established a differentiation method for homogeneous α7 integrin-positive human skeletal muscle stem cell (α7(+)hSMSC)-derived osteoblast-like cells with bone morphogenetic protein (BMP)-2. To explore the early signaling cascade for osteoblastic differentiation, we examined the upregulation of autophagy-related gen...
journal_title:Experimental cell research
pub_type: 杂志文章,收录出版
doi:10.1016/j.yexcr.2016.07.002
更新日期:2016-09-10 00:00:00
abstract::The biosynthesis of type XI and type II collagens was examined using a stable rat chondrocyte cell line established by W. E. Horton et al. (1988, Exp. Cell Res. 178, 457-468.). These cells (IRC; immortalized rat chondrocytes) were created by transformation with a murine retrovirus carrying the v-myc and v-raf oncogene...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1994.1169
更新日期:1994-07-01 00:00:00
abstract::Previous hybrid studies involving fusion of normal with immortal human cells indicated that the phenotype of cellular senescence is dominant and that immortality results from recessive changes in normal growth regulatory genes. We have further assigned 28 different immortal human cell lines to at least four complement...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(91)90179-x
更新日期:1991-01-01 00:00:00
abstract::The objective of this study was to explore the potential use of self-assembled monolayers (SAMs) of alkylamine and arylalkyamine as well-defined, homogeneous, tailored in vitro model surfaces for exploring the effect of hydrodynamic flow on morphology and strength of adhesion of human umbilical vein endothelial cells....
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1998.4156
更新日期:1998-10-10 00:00:00
abstract::Human skin fibroblasts deficient in peroxisome biogenesis were transformed by transfecting SV40 ori- DNA with the use of an electroporator, and the biochemical, immunocytochemical, and cytogenetic properties of the transformants were analyzed. Cells (1 x 10(6)) from a patient with Zellweger syndrome and one with neona...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(92)90278-g
更新日期:1992-08-01 00:00:00
abstract::Nucleoporins (Nups) are a large and diverse family of proteins that mediate nucleocytoplasmic transport at interphase of vertebrate cells. Nups also function in mitosis progression. However, whether Nups are involved in oocyte meiosis progression is still rarely known. In this study, we delineated the roles and regula...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2018.09.004
更新日期:2018-10-15 00:00:00
abstract::Tob, a member of the Tob and BTG antiproliferative protein family, plays an important role in many cellular processes including cell proliferation. In this study, we have addressed molecular mechanisms regulating subcellular localization of Tob. Treatment with leptomycin B, an inhibitor of nuclear export signal (NES) ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2003.12.016
更新日期:2004-04-15 00:00:00
abstract::It has been reported that cholesterol-lowing agents can ameliorate severity of myocarditis. However, the beneficial effect of the agents has been claimed to be independent of cholesterol reduction as there is no significant change in the plasma cholesterol level in myocarditis. In the present study, using experimental...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2020.112169
更新日期:2020-10-01 00:00:00
abstract::In contrast to extensive studies on the roles of molecular chaperones, such as heat shock proteins, there are only a few reports about the roles of GRP78/BiP, an endoplasmic reticulum (ER) stress-induced molecular chaperone, in mammalian cell responses to DNA-damaging stresses. To investigate whether GRP78/BiP is invo...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2005.01.002
更新日期:2005-05-01 00:00:00
abstract::This study reports on the specific binding of [3H]heparin to human adrenocortical carcinoma cell line SW-13. Heparin binding to SW-13 cells is specific, saturable, and time- and temperature-dependent with maximum binding occurring between 90 and 120 min at 22 degrees C. Scatchard analysis revealed two classes of bindi...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(90)90099-v
更新日期:1990-04-01 00:00:00
abstract::Prior studies have shown that synthetic peptides representing the domain of thrombin responsible for high-affinity binding to fibroblasts stimulate chemotactic and cell proliferative signals through a nonproteolytic mechanism. One of these peptides, TP508, has recently been shown to be chemotactic for neutrophils, to ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1998.4372
更新日期:1999-03-15 00:00:00
abstract::Exponentially growing Chinese hamster V79-171b lung fibroblasts seeded at high density on plastic (approximately 7 x 10(3) cells/cm2) flatten, elongate, and produce significant amounts of extracellular fibronectin. When lysed in weak alkali/high salt, the rate of DNA denaturation following exposure to ionizing radiati...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(91)90105-4
更新日期:1991-04-01 00:00:00
abstract::JANEX-1/WHI-P131, a selective Janus kinase 3 (JAK3) inhibitor, has been shown to delay the onset of diabetes in the NOD mouse model. However, the molecular mechanism by which JANEX-1 protects pancreatic beta-cells is unknown. In the current study, we investigated the role of JANEX-1 on interleukin (IL)-1beta and inter...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2009.04.021
更新日期:2009-07-15 00:00:00
abstract::It has previously been shown by immunocytochemistry that the inactive X chromosome (Xi) in somatic cells of human and mouse females is marked by underacetylation of histone H4. It has been suggested that this may be important for transcriptional silencing of genes on Xi. We have now investigated X-inactivation in meio...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1996.3394
更新日期:1997-02-01 00:00:00
abstract::The effect of retinoic acid treatment on cell attachment to plastic substrates precoated with fibronectin, gelatin, laminin, and type IV collagen was investigated. Both retinoic acid-treated and control cells attached efficiently to fibronectin or gelatin substrates without any significant difference. In contrast, ret...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(87)90285-0
更新日期:1987-12-01 00:00:00
abstract::Epithelial-mesenchymal transition (EMT) plays an important role in breast cancer cell metastasis. Both (megakaryoblastic leukemia)/myocardin-like 1 (MKL-1) and Signal transducer and activator of transcription 3 (STAT3) have been implicated in the control of cellular metabolism, survival and growth. Our previous study ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2017.05.007
更新日期:2017-08-01 00:00:00
abstract::The process of tumor cell invasion of the basement membrane is proposed to consist of three steps: attachment, local proteolysis and migration. 12-(S)-HETE, a 12-lipoxygenase metabolite of arachidonic acid, upregulates surface expression of integrin cytoadhesins and an autocrine motility factor receptor, suggesting th...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1994.1240
更新日期:1994-09-01 00:00:00
abstract::An earlier report indicated that a 26-amino-acid peptide (SA), comprised of the nuclear localization signal (NLS) of fibroblast growth factor-1 (FGF-1) and a membrane-permeable peptide, was able to stimulate DNA synthesis after it was taken up by NIH3T3 fibroblasts. Here, we report that SA, but not a mutant with the N...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1998.4176
更新日期:1998-09-15 00:00:00
abstract::We have tested whether life-long caloric restriction (CR) slows or delays the age-related loss of cellular replicative potential that occurs during normal aging in ad libitum (AL) fed mice. Both mean and maximum life spans of the restricted animals (60% of AL intake) were significantly extended 30-40% by CR treatment....
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1995.1091
更新日期:1995-04-01 00:00:00
abstract::Individual tumor cells utilize one of two modes of motility to invade the extracellular matrix, mesenchymal or amoeboid. We have determined that the diterpenoid genkwanine M (GENK) enhances the mesenchymal mode of cell motility that is intrinsic to HT-1080 osteosarcoma cells, stimulates a mesenchymal mode of motility ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2013.01.005
更新日期:2013-04-01 00:00:00
abstract::Template-activating factors I (TAF-I) alpha and beta have been identified as chromatin remodeling factors from human HeLa cells. TAF-I beta corresponds to the protein encoded by the set gene, which was found in an acute undifferentiated leukemia as a fusion version with the can gene via chromosomal translocation. To d...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1997.3930
更新日期:1998-05-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) are frequently used as a therapeutic, but reliable imaging technique to longitudinally evaluate the engraftment of transplanted cells is inadequate. For magnetic resonance imaging (MRI), it is essential to understand the technical competence of in vitro stem cells labeling with iron oxide...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2016.12.025
更新日期:2017-02-01 00:00:00
abstract::Ovarian cancer (OC) is the leading cause of death in gynecologic diseases in which there is evidence for a complex chemokine network. Chemokines are a family of proteins that play an important role in tumor progression influencing cell proliferation, angiogenic/angiostatic processes, cell migration and metastasis, and...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2005.04.024
更新日期:2005-08-15 00:00:00
abstract::Type VI collagen is a nonfibrillar collagen present as a network throughout the chick secondary stroma. Immunolocalization of type VI collagen both in the chick corneal stroma and in other systems demonstrates that type VI collagen is present associated with cells and between striated fibrils. We hypothesize that type...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(92)90200-r
更新日期:1992-06-01 00:00:00
abstract::Pancreatic cancer is characterized by aggressive local invasion and early metastasis formation. Active migration of the pancreatic cancer cells is essential for these processes. We have shown previously that the pancreatic cancer cells lines CFPAC1 and IMIM-PC2 show high migratory activity, and we have investigated he...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2014.04.022
更新日期:2014-08-15 00:00:00
abstract::Mammalian enabled (MENA), usually known as a direct regulator of microfilament polymerization and bundling, promotes metastasis in various cancers. Here we focus on the role of MENA in hepatocellular carcinoma (HCC) metastasis and the relevant mechanism from the view of RhoA activity regulation. By HCC tissue microarr...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2014.05.010
更新日期:2014-09-10 00:00:00
abstract::Rat pheochromocytoma cells, PC12 cells, undergo differentiation in response to nerve growth factor (NGF). Although the Ras-MAP kinase signaling pathway has been shown to play a central role in the response to NGF, the precise mechanism which induces differentiation remains unclarified. Recently, several gamma-lactam-r...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1997.3615
更新日期:1997-08-01 00:00:00
abstract::Apoptosis is characterized by typical morphological changes and most frequently fragmentation of DNA into oligonucleosome-size fragments. In order to investigate whether an alteration in the mechanisms involved in the process of apoptosis could contribute to cellular resistance, induction of apoptosis was studied in a...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1995.1148
更新日期:1995-05-01 00:00:00
abstract::Galectin-3, a beta-galactoside-binding protein, has been shown to be involved in multiple biological processes through interaction with its complementary glycoconjugates. Here we provide the first evidence of galectin-3 as a mitogen. Incubation of quiescent cultures of normal human lung fibroblast IMR-90 cells with re...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1998.4253
更新日期:1998-12-15 00:00:00