Inflammation-driven colon neoplasmatogenesis in uPA-deficient mice is associated with an increased expression of Runx transcriptional regulators.

Abstract:

:Deregulation of the bone morphogenetic protein (BMP) pathway has been documented in colorectal cancer (CRC). Previously, we investigated possible associations between urokinase-type plasminogen activator (uPA) deficiency and expression of extracellular constituents of BMP signaling in a newly developed mouse model of inflammation-driven intestinal neoplasmatogenesis, in which chronic colitis and CRC are induced using dextran sodium sulfate (DSS). In this report, we explored the contribution of intracellular components of Smad-mediated BMP signal transduction using the same model. Interestingly, upon DSS treatment, we noticed an overexpression of Runx1/2/3 transcription factors in both wild-type and uPA-deficient mice. Moreover, Runx1 and Runx2 expression levels exhibited an even higher increase in DSS-treated/uPA-deficient mice as compared to DSS-treated/wild-type animals. In all experimental conditions, in situ investigation of Runx-expressing cell types, revealed detection of all three Runx in the immune cells, yet in the DSS-treated/uPA-deficient mice Runx1 and Runx2 were also identified in the preneoplastic epithelium of advanced high-grade dysplasia and carcinoma in-situ colonic lesions. Finally, the uPA-deficient pro-tumorigenic colitic microenvironment exhibited increased levels of the Runx-induced target genes Snai2, Bim and Claudin1, known to have a role in tumor development and progression. These findings suggest that the absence of uPA correlates with increased levels of Runx transcriptional regulators in a way that promotes inflammation-associated carcinogenesis.

journal_name

Exp Cell Res

authors

Afaloniati H,Karagiannis GS,Hardas A,Poutahidis T,Angelopoulou K

doi

10.1016/j.yexcr.2017.10.025

subject

Has Abstract

pub_date

2017-12-15 00:00:00

pages

257-264

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(17)30575-X

journal_volume

361

pub_type

杂志文章
  • PLCgamma participates in insulin stimulation of glucose uptake through activation of PKCzeta in brown adipocytes.

    abstract::Based on recent studies showing that PLCgamma associates to insulin receptor, we investigated its role in insulin stimulation of glucose transport in brown adipocytes. Insulin stimulation induced rapid PLCgamma association to phosphorylated insulin receptor, and activation of PLCgamma, as assessed by the mobilization ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.2002.5570

    authors: Lorenzo M,Teruel T,Hernandez R,Kayali AG,Webster NJ

    更新日期:2002-08-15 00:00:00

  • The β-catenin signaling pathway induces aggressive potential in breast cancer by up-regulating the chemokine CCL5.

    abstract::β-Catenin signaling plays a pivotal role in the genesis of a variety of malignant tumors, but its role in breast cancer has not been fully elucidated. Here, we examined whether deregulation of β-catenin signaling is related to the aggressive characteristics of certain types of breast cancers. Analysis of cytokine leve...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2015.09.003

    authors: Yasuhara R,Irié T,Suzuki K,Sawada T,Miwa N,Sasaki A,Tsunoda Y,Nakamura S,Mishima K

    更新日期:2015-10-15 00:00:00

  • Growth stimulation by retinol apparently independent of EGF receptor.

    abstract::A respiratory epithelial cell line M3E3/C3, derived from fetal Syrian hamster lung, grew very slowly in RPMI 1640 medium with serum concentrations as low as 1.5%. Under these conditions the simultaneous action of epidermal growth factor (EGF) and vitamin A (retinol) was apparently a combination of growth enhancement a...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(86)90578-1

    authors: Böning W,Kakunaga T,Emura M,Mohr U

    更新日期:1986-03-01 00:00:00

  • Neurite extension by neuroblastoma cells on substratum-bound fibronectin's cell-binding fragment but not on the heparan sulfate-binding protein, platelet factor-4.

    abstract::Human and rat neuroblastoma cells extend neurites over plasma fibronectin (pFN)-coated substrata. For resolution of which fibronectin binding activities (the cell-binding domain (CBD), the heparan sulfate-binding domains, or a combination of the two) are responsible for neurite outgrowth, CBD was prepared free of hepa...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(85)90464-1

    authors: Tobey SL,McClelland KJ,Culp LA

    更新日期:1985-06-01 00:00:00

  • Myristoyl-coA:protein N-myristoyltransferase from bovine cardiac muscle: molecular cloning, kinetic analysis, and in vitro proteolytic cleavage by m-calpain.

    abstract::Myristoyl-CoA:protein N-myristoyltransferase (NMT) catalyzes the attachment of myristate onto the amino terminal glycine residue of select polypeptides. Cardiac tissue expresses high levels of cAMP-dependent protein kinase whose catalytic subunit is myristoylated; however, cardiac muscle extracts were found to contain...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1998.4021

    authors: Raju RV,Kakkar R,Datla RS,Radhi J,Sharma RK

    更新日期:1998-05-25 00:00:00

  • Multiple roles of alpha-smooth muscle actin in mechanotransduction.

    abstract::Alpha-smooth muscle actin (SMA), an actin isoform that contributes to cell-generated mechanical tension, is normally restricted to cells of vascular smooth muscle, but SMA can also be expressed in certain non-muscle cells, most notably myofibroblasts. These cells are present in healing wounds, scars, and fibrocontract...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审

    doi:10.1016/j.yexcr.2005.11.004

    authors: Wang J,Zohar R,McCulloch CA

    更新日期:2006-02-01 00:00:00

  • Mononuclear cell-mediated modulation of synovial cell metabolism. I. Collagen synthesis.

    abstract::Human PHA-stimulated mononuclear cells produce a factor which inhibits synovial cell collagen and non-collagen protein synthesis, whereas it enhances hyaluronic acid (HA) production. Indomethacin (10(-4)-10(-6) M), a cyclo-oxygenase inhibitor, suppresses this effect, suggesting that the mechanism is prostaglandin-medi...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(85)90431-8

    authors: Pujol JP,Penfornis H,Arenzana-Seisdedos F,Bocquet J,Farjanel J,Rattner A,Brisset M,Virelizier JL,Beliard R,Loyau G

    更新日期:1985-05-01 00:00:00

  • Critical roles of Src family tyrosine kinases in excitatory neuronal differentiation of cultured embryonic stem cells.

    abstract::Embryonic stem (ES) cells have been tested for potential cell transplantation therapy for CNS disorders. Understanding their differentiation mechanism and identifying factors involved in driving excitatory and inhibitory neuron lineages should enhance the efficacy and efficiency of the cell transplantation therapy. We...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2006.06.022

    authors: Theus MH,Wei L,Francis K,Yu SP

    更新日期:2006-10-01 00:00:00

  • Stability and nuclear distribution of mammalian replication protein A heterotrimeric complex.

    abstract::Replication protein A (RPA), a stable complex of three polypeptides, is the single-stranded DNA-binding protein essential for DNA replication in eukaryotic cells. Previous studies of the subcellular distribution and stability of the RPA heterotrimer during the mammalian cell cycle have produced conflicting results. He...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1999.4770

    authors: Dimitrova DS,Gilbert DM

    更新日期:2000-02-01 00:00:00

  • Subcellular distribution of distinct nucleolin subfractions recognized by two monoclonal antibodies.

    abstract::Monoclonal antibodies binding to different domains of nucleolin have been used to localize nucleolin in tissue culture cells of Xenopus laevis. The monoclonal antibody b6-6E7 binds to an epitope in the N-terminal domain, which contains arrays of phosphorylation consensus sites. This monoclonal antibody binds to nucleo...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1997.3878

    authors: Schwab MS,Gossweiler U,Dreyer C

    更新日期:1998-03-15 00:00:00

  • Interleukin 10 enhanced CD8+ T cell activity and reduced CD8+ T cell apoptosis in patients with diffuse large B cell lymphoma.

    abstract::The pleiotropic cytokine interleukin (IL)-10 is best characterized by its ability to downregulate inflammation and promote peripheral tolerance. On the other hand, IL-10 was also found to maintain the effector response of CD8+ T cells and promote the expansion of tumor-resident CD8+ T cells. In diffuse large B cell ly...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2017.08.036

    authors: Qiu H,Hu X,Gao L,Chen L,Chen J,Yuan J,Huang C,Xu X,Yang J

    更新日期:2017-11-15 00:00:00

  • An investigation of adhesion and detachment in slime mould amoebae using columns of hydrophobic beads.

    abstract::Vegetative amoebae of Dictyostelium discoideum (strain AX2) adhere to columns of hydrophobic beads (Bio-Beads SM-2) which have been coated with axenic growth medium. This binding is blocked by sodium azide and other inhibitors of aerobic respiration. Bound cells are not detached by lowering the temperature but they ca...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(84)90235-0

    authors: Glynn PJ,Clarke KR

    更新日期:1984-05-01 00:00:00

  • Actin organization during the cell cycle in meristematic plant cells. Actin is present in the cytokinetic phragmoplast.

    abstract::The distribution and organisation of F-actin during the cell cycle of meristematic root-tip cells of Allium was investigated using a rhodamine-labelled phalloidin to stain F-actin in isolated cell preparations. Such preparations could, in addition, be stained for tubulin by immunofluorescence, enabling a comparison be...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(85)90277-0

    authors: Clayton L,Lloyd CW

    更新日期:1985-01-01 00:00:00

  • Screening of genes involved in cell migration in Dictyostelium.

    abstract::A single cell of wild-type Dictyostelium discoideum forms a visible colony on a plastic dish in several days, but due to enhanced cell migration, amiB-null mutant cells scatter over a large area and do not form noticeable colonies. Here, with an aim to identify genes involved in cell migration, we isolated suppresser ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2007.12.002

    authors: Nagasaki A,Uyeda TQ

    更新日期:2008-03-10 00:00:00

  • SIRT6 inhibits TNF-α-induced inflammation of vascular adventitial fibroblasts through ROS and Akt signaling pathway.

    abstract::SIRT6, with both deacetylase and ADP-ribosyltransferase activities, is predominantly expressed in the nucleus. It has been revealed that SIRT6 regulates various biological functions including metabolism, aging and stress resistance. This study aims to investigate the role of SIRT6 in vascular inflammation and it molec...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2017.05.001

    authors: He Y,Xiao Y,Yang X,Li Y,Wang B,Yao F,Shang C,Jin Z,Wang W,Lin R

    更新日期:2017-08-01 00:00:00

  • Acute myocardial infarction leads to upregulation of the IGF-1 autocrine system, DNA replication, and nuclear mitotic division in the remaining viable cardiac myocytes.

    abstract::Insulin-like growth factor-1 (IGF-1) and its receptor (IGF-1R) are required for cell proliferation in vitro, raising the possibility that an autocrine IGF-1-IGF-1R system may be present in vivo and become activated in the viable ventricular myocytes shortly after infarction. Therefore, following the in vivo documentat...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1994.1224

    authors: Reiss K,Kajstura J,Zhang X,Li P,Szoke E,Olivetti G,Anversa P

    更新日期:1994-08-01 00:00:00

  • Stable intermediates and holdpoints in the rapid differentiation of Naegleria.

    abstract::The rapidity of the optional 90-min differentiation of Naegleria gruberi from amoebae to flagellates suggests the possibility of a free-running cascade of events from initiating stimulus through gene expression to organelle assembly and cell morphogenesis. Instead our experiments reveal two points early in the differe...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1998.4121

    authors: Fulton C,Lai EY

    更新日期:1998-08-01 00:00:00

  • Permeable FGF-1 nuclear localization signal peptide stimulates DNA synthesis in various cell types but is cell-density sensitive and unable to support cell proliferation.

    abstract::An earlier report indicated that a 26-amino-acid peptide (SA), comprised of the nuclear localization signal (NLS) of fibroblast growth factor-1 (FGF-1) and a membrane-permeable peptide, was able to stimulate DNA synthesis after it was taken up by NIH3T3 fibroblasts. Here, we report that SA, but not a mutant with the N...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1998.4176

    authors: Komi A,Suzuki M,Imamura T

    更新日期:1998-09-15 00:00:00

  • Transforming NRK cells with avian sarcoma virus reduces the extracellular Ca2+ requirement without affecting the calcicalmodulin requirement for the G1/S transition.

    abstract::NRK rat cells infected with a transformation-defective, temperature-sensitive (ts) mutant of the avian sarcoma virus could not proliferate in Ca2+-deficient medium at a nonpermissive temperature (40 degrees C) that inactivated the viral pp60v-src-transforming product and rendered the cells phenotypically untransformed...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(85)90140-5

    authors: Durkin JP,Whitfield JF

    更新日期:1985-04-01 00:00:00

  • Senescence stimulates U937-endothelial cell interactions.

    abstract::Progressive pathophysiologic modifications of endothelial cells are associated with aging. In vitro, endothelial cell senescence is accompanied by the failure to proliferate as well as by perturbations in gene expression. Here we show that (i) senescence enhances monoblastoid U937 cell adhesion to the endothelial mono...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1993.1246

    authors: Maier JA,Statuto M,Ragnotti G

    更新日期:1993-09-01 00:00:00

  • Cyclic AMP in ovarian cancer cells both inhibits proliferation and increases c-KIT expression.

    abstract::C-KIT encodes a tyrosine kinase receptor (KIT) that, when activated by its ligand (KL), stimulates proliferation, differentiation, migration, and survival. Greater than 70% of epithelial ovarian cancers coexpress c-KIT and KL. C-KIT and KL expression levels have been shown to be up-regulated by cAMP in some cell types...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.2001.5426

    authors: Shaw TJ,Keszthelyi EJ,Tonary AM,Cada M,Vanderhyden BC

    更新日期:2002-02-01 00:00:00

  • Retinal differentiation of human bone marrow-derived stem cells by co-culture with retinal pigment epithelium in vitro.

    abstract::The goal of this study was to assess the in vitro differentiation capacity of human bone marrow-derived stem cells (hBMSCs) along retinal lineages. Mononuclear cells (MNC) were isolated from bone marrow (BM) and mobilized peripheral blood (mPB) using Ficoll-Paque density gradient centrifugation, and were sorted by mag...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2015.02.001

    authors: Mathivanan I,Trepp C,Brunold C,Baerlocher G,Enzmann V

    更新日期:2015-04-10 00:00:00

  • Secreted collagen induced by ascorbic acid in L5 cloned muscle cultures does not affect acetylcholine receptor expression.

    abstract::Previous studies have shown that ascorbic acid increases both the total surface acetylcholine receptor (AChR) expression and the mRNA for the alpha-subunit of this receptor in myotubes of cloned L5 muscle cultures. Since ascorbic acid increases collagen synthesis in fibroblasts, we studied the effect of ascorbic acid ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1993.1287

    authors: Liu E,Minor RR,Horovitz O,Wootton JA,Podleski TR,Salpeter MM

    更新日期:1993-11-01 00:00:00

  • The long non-coding RNA ILF3-AS1 increases the proliferation and invasion of retinoblastoma through the miR-132-3p/SMAD2 axis.

    abstract::A great deal of evidence suggests that long non-coding RNAs (lncRNAs) function in the tumorigenesis of retinoblastoma (RB). However, the roles of lncRNA ILF3-AS1 in RB are still unclear. In the present study, our work revealed that the lncRNA ILF3-AS1 was increased in both RB tissues and cell lines. Repression of ILF3...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2020.112087

    authors: Han S,Song L,Chen Y,Hou M,Wei X,Fan D

    更新日期:2020-08-15 00:00:00

  • Coagulation factor Xa drives tumor cells into apoptosis through BH3-only protein Bim up-regulation.

    abstract::Coagulation Factor (F)Xa is a serine protease that plays a crucial role during blood coagulation by converting prothrombin into active thrombin. Recently, however, it emerged that besides this role in coagulation, FXa induces intracellular signaling leading to different cellular effects. Here, we show that coagulation...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2007.04.014

    authors: Borensztajn KS,Bijlsma MF,Groot AP,Brüggemann LW,Versteeg HH,Reitsma PH,Peppelenbosch MP,Spek CA

    更新日期:2007-07-15 00:00:00

  • Resveratrol decreases TNFα-induced ICAM-1 expression and release by Sirt-1-independent mechanism in intestinal myofibroblasts.

    abstract::Up-regulation of intercellular adhesion molecule-1 (ICAM-1) and its soluble form are involved in the chronic inflammation. For the first time, we demonstrated that resveratrol (RE), a natural polyphenol with antioxidant and anti-inflammatory properties, reduces the increase of expression and release of ICAM-1, due to ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2019.06.024

    authors: Domazetovic V,Bonanomi AG,Stio M,Vincenzini MT,Iantomasi T

    更新日期:2019-09-15 00:00:00

  • Caloric restriction ameliorates cardiomyopathy in animal model of diabetes.

    abstract:BACKGROUND:The db/db mouse is an animal model of diabetes in which leptin receptor activity is deficient resulting accelerated cardiomyopathy when exposed to angiotensin (AT). Toll-like receptors 4 and 2 (TLR4, TLR2) are pattern recognition receptors, that recognize pathogen-associated molecular patterns and exacerbate...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2016.11.016

    authors: Cohen K,Waldman M,Abraham NG,Laniado-Schwartzman M,Gurfield D,Aravot D,Arad M,Hochhauser E

    更新日期:2017-01-01 00:00:00

  • Transmyocardial drilling revascularization combined with heparinized bFGF-incorporating stent activates resident cardiac stem cells via SDF-1/CXCR4 axis.

    abstract:OBJECTIVE:To investigate whether transmyocardial drilling revascularization combined with heparinized basic fibroblast growth factor (bFGF)-incorporating degradable stent implantation (TMDRSI) can promote myocardial regeneration after acute myocardial infarction (AMI). METHODS:A model of AMI was generated by ligating ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2011.11.009

    authors: Zhang GW,Wen T,Gu TX,Li-Ling J,Wang C,Zhao Y,Liu J,Wang Y,Liu TJ,Lü F

    更新日期:2012-02-15 00:00:00

  • Dysregulation of Ack1 inhibits down-regulation of the EGF receptor.

    abstract::The protein tyrosine kinase Ack1 has been linked to cancer when over-expressed. Ack1 has also been suggested to function in clathrin-mediated endocytosis and in down-regulation of the epidermal growth factor (EGF) receptor (EGFR). We have studied the intracellular localization of over-expressed Ack1 and found that Ack...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2007.12.017

    authors: Grøvdal LM,Johannessen LE,Rødland MS,Madshus IH,Stang E

    更新日期:2008-04-01 00:00:00

  • LincRNA-p21 knockdown enhances radiosensitivity of hypoxic tumor cells by reducing autophagy through HIF-1/Akt/mTOR/P70S6K pathway.

    abstract::Hypoxic conditions are common in solid tumors and have a significant effect on tumor progression, therapeutic and prognosis. Long noncoding RNAs (lncRNAs) are longer than 200 nucleotides and cannot be translated into proteins, which play important roles in some diseases including cancer. Although previous analysis hav...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2017.06.016

    authors: Shen Y,Liu Y,Sun T,Yang W

    更新日期:2017-09-15 00:00:00