Possible involvement of the interaction of the alpha 5 subunit of alpha 5 beta 1 integrin with the synergistic region of the central cell-binding domain of fibronectin in cells to fibronectin binding.


:The interaction between the central cell-binding domain (CBD) of fibronectin (FN) and its receptor integrin alpha 5 beta 1 was analyzed by both ligand-binding and cell adhesion assays. The ligands used were a CBD fragment (pCBD) of human plasma fibronectin and its recombinant versions including wild type CBD (wtCBD) and its two mutants (CBD-I, lacking the integrin recognition sequence Arg-Gly-Asp from wtCBD and CBD-II, missing the synergistic regions). The ligand-binding assay showed that CBD-I and CBD-II bind to the receptor, although the binding ability of the former was weaker than that of the latter. The affinity of pCBD to the receptor was much higher than the two mutants. The cell adhesion assay also revealed that cells were able to attach and spread on CBD-I to the same extent as on CBD-II, although the extent of spreading on the two mutant polypeptides was less than 4.1% of pCBD or wtCBD. On the other hand, beta 1-dependent cell spreading on CBD-II was not inhibited by the monoclonal antibody specific for the alpha 5 subunit, while that on CBD-I, wtCBD, or pCBD was inhibited by the same antibody. The present study suggests that the alpha 5 subunit does not participate in direct binding to the Arg-Gly-Asp site in CBD when cells adhere to FN through the integrin alpha 5 beta 1, but that it is involved in the interaction with the synergistic regions of CBD, which then enhances the binding of the beta 1 subunit to the Arg-Gly-Asp sequence containing CBD.


Exp Cell Res


Obara M,Yoshizato K




Has Abstract


1995-01-01 00:00:00














  • Observations on the vimentin-10-NM filaments during mitosis in BHK21 cells.

    abstract::Mitotic BHK21 cells were examined by immunofluorescence optical sectioning after staining with anti-vimentin. Throughout all stages of mitosis, intact 10-nm filaments were observed to form a cage around the mitotic spindle. These observations were confirmed by serial section electron microscopy which demonstrated asse...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Blose SH,Bushnell A

    更新日期:1982-11-01 00:00:00

  • Activation of ROCK by RhoA is regulated by cell adhesion, shape, and cytoskeletal tension.

    abstract::Adhesion to the extracellular matrix regulates numerous changes in the actin cytoskeleton by regulating the activity of the Rho family of small GTPases. Here, we report that adhesion and the associated changes in cell shape and cytoskeletal tension are all required for GTP-bound RhoA to activate its downstream effecto...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Bhadriraju K,Yang M,Alom Ruiz S,Pirone D,Tan J,Chen CS

    更新日期:2007-10-01 00:00:00

  • The synthetic tubulysin derivative, tubugi-1, improves the innate immune response by macrophage polarization in addition to its direct cytotoxic effects in a murine melanoma model.

    abstract::Synthetic tubugis are equally potent but more stable than their natural forms. Their anticancer potential was estimated on a solid melanoma in vitro and in vivo. Tubugi-1 induced the apoptosis in B16 cells accompanied with strong intracellular production of reactive species, subsequently imposing glutathione and thiol...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Drača D,Mijatović S,Krajnović T,Pristov JB,Đukić T,Kaluđerović GN,Wessjohann LA,Maksimović-Ivanić D

    更新日期:2019-07-15 00:00:00

  • Fibroblast growth factors 1 and 2 inhibit adipogenesis of human bone marrow stromal cells in 3D collagen gels.

    abstract::Multipotent human bone marrow stromal cells (hBMSCs) are the common progenitors of osteoblasts and adipocytes. A shift in hBMSC differentiation in favor of adipogenesis may contribute to the bone loss and marrow fat accumulation observed in aging and osteoporosis. Hence, the identification of factors modulating marrow...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Le Blanc S,Simann M,Jakob F,Schütze N,Schilling T

    更新日期:2015-11-01 00:00:00

  • Localization of nucleoside triphosphatase activity to the inner nuclear envelope and associated heterochromatin.

    abstract::Nuclear-envelope nucleoside triphosphatase activity (NTPase), an enzymatic activity thought to participate in RNA transport, was localized in rat liver in situ after brief perfusion with 3% paraformaldehyde. Reaction product was distributed along the nucleoplasmic side of the nuclear envelope (NE) in heterochromatin, ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Clawson GA,Friend DS,Smuckler EA

    更新日期:1984-11-01 00:00:00

  • Xenopus single-minded (xSim) is a nuclear factor allowing nuclear translocation of its cytoplasmic partner xArnt.

    abstract::Transcription factors belonging to the basic helix-loop-helix Per-Arnt-Sim (bHLH/PAS) family control a wide variety of biological processes in mammalian and/or Drosophila. We have previously isolated bHLH/PAS Xenopus amphibian homologs of Single-minded (xSim) and aryl receptor nuclear translocator (xArnt) and characte...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Coumailleau P,Bollérot K,Lecomte C,Angelier N

    更新日期:2003-07-15 00:00:00

  • Vascular endothelial growth factor promotes cardiac stem cell migration via the PI3K/Akt pathway.

    abstract::VEGF is a major inducer of angiogenesis. However, the homing role of VEGF for cardiac stem cells (CSCs) is unclear. In in vitro experiments, CSCs were isolated from the rat hearts, and a cellular migration assay was performed using a 24-well transwell system. VEGF induced CSC migration in a concentration-dependent man...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Tang J,Wang J,Kong X,Yang J,Guo L,Zheng F,Zhang L,Huang Y,Wan Y

    更新日期:2009-12-10 00:00:00

  • A RanBP1 mutation which does not visibly affect nuclear import may reveal additional functions of the ran GTPase system.

    abstract::Ran, a nuclear GTPase, and a number of interacting proteins, including regulators RanGEF1 and RanGAP1, are involved in nucleocytoplasmic transport. We have identified a new temperature-sensitive mutation in budding yeast YRB1 gene, which encodes Ran-binding protein-1 (RanBP1). In contrast to other yrb1 alleles, the ne...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ouspenski II

    更新日期:1998-10-10 00:00:00

  • Polo-like kinase 3 is Golgi localized and involved in regulating Golgi fragmentation during the cell cycle.

    abstract::The Golgi apparatus undergoes extensive fragmentation during mitosis in animal cells. Protein kinases play a critical role during fragmentation of the Golgi apparatus. We reported here that Polo-like kinase 3 (Plk3) may be an important mediator during Golgi breakdown. Specifically, Plk3 was concentrated at the Golgi a...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ruan Q,Wang Q,Xie S,Fang Y,Darzynkiewicz Z,Guan K,Jhanwar-Uniyal M,Dai W

    更新日期:2004-03-10 00:00:00

  • Effect of shear stress on cytosolic Ca2+ of calf pulmonary artery endothelial cells.

    abstract::The purpose of the present study was to determine if hemodynamic shear stress increases free cytosolic Ca2+ concentration ([Ca2+]i) of cultured pulmonary artery endothelial cells exposed to steady laminar fluid flow in a parallel plate chamber. Average [Ca2+]i was estimated by measuring cell-associated fura-2 fluoresc...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Schilling WP,Mo M,Eskin SG

    更新日期:1992-01-01 00:00:00

  • p120 serine and threonine phosphorylation is controlled by multiple ligand-receptor pathways but not cadherin ligation.

    abstract::p120-catenin (p120) regulates cadherin turnover and is required for cadherin stability. Extensive and dynamic phosphorylation on tyrosine, serine and threonine residues in the N-terminal regulatory domain has been postulated to regulate p120 function, possibly through modulation of the efficiency of p120/cadherin inte...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Xia X,Carnahan RH,Vaughan MH,Wildenberg GA,Reynolds AB

    更新日期:2006-10-15 00:00:00

  • The cytoplasmic pH, ATP content and total protein synthesis rate during heat-shock protein inducing treatments in yeast.

    abstract::In S. cerevisiae the induction of heat-shock protein (HSP) synthesis is accompanied by a decrease in the cytoplasmic and vacuolar pH as determined by means of [31P]NMR spectroscopy. The relationship of HSP synthesis and acidification of the cytoplasmic pH is dose-dependent under a variety of treatments (temperature in...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Weitzel G,Pilatus U,Rensing L

    更新日期:1987-05-01 00:00:00

  • Multiple proteases are involved in thymocyte apoptosis.

    abstract::To investigate the involvement of proteases in apoptosis, rat thymocytes were treated with the glucocorticoid hormone methylprednisolone or the topoisomerase II inhibitor etoposide in the presence of selective substrate inhibitors of either interleukin-1 beta-converting enzyme (ICE), (Z-Val-Ala-Asp-chloromethylketone,...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zhivotovsky B,Gahm A,Ankarcrona M,Nicotera P,Orrenius S

    更新日期:1995-12-01 00:00:00

  • Investigation of the role of G1/S cell cycle mediators in cellular senescence.

    abstract::Cellular senescence is a state of irreversible cell cycle arrest in which normal cells at the end of their lifespan fail to enter into DNA synthesis upon serum or growth factor stimulation. We examined whether proteins required for G1/S cell cycle progression were irreversibly down-regulated in senescent human fibrobl...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Afshari CA,Vojta PJ,Annab LA,Futreal PA,Willard TB,Barrett JC

    更新日期:1993-12-01 00:00:00

  • Plakophilin3 loss leads to an increase in lipocalin2 expression, which is required for tumour formation.

    abstract::An increase in tumour formation and metastasis are observed upon plakophilin3 (PKP3) loss. To identify pathways downstream of PKP3 loss that are required for increased tumour formation, a gene expression analysis was performed, which demonstrated that the expression of lipocalin2 (LCN2) was elevated upon PKP3 loss and...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Basu S,Chaudhary N,Shah S,Braggs C,Sawant A,Vaz S,Thorat R,Gupta S,Dalal SN

    更新日期:2018-08-15 00:00:00

  • Alpha-factor inhibition of the rate of cell passage through the "start" step of cell division in Saccharomyces cerevisiae yeast: estimation of the division delay per alpha-factor.receptor complex.

    abstract::A highly sensitive, kinetically unambiguous assay for alpha-factor-induced delay of cell passage through the "start" step of cell division in yeast is presented. The assay employs perfusion with periodic microscopy to monitor the bud emergence kinetics on the 20% of cells within an exponentially growing population whi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Moore SA

    更新日期:1987-08-01 00:00:00

  • Alfalfa Mob1-like proteins are involved in cell proliferation and are localized in the cell division plane during cytokinesis.

    abstract::Mps-one-binder (Mob) proteins play a crucial role in yeast cytokinesis. After cloning two Mob1-like genes, MsMob1-A and MsMob1-B from alfalfa (Medicago sativa L.) we show that, although they are constitutively expressed in roots, stems, leaves, flowers and pods, their transcripts and proteins are mostly produced in ac...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Citterio S,Piatti S,Albertini E,Aina R,Varotto S,Barcaccia G

    更新日期:2006-04-15 00:00:00

  • Screening of genes involved in cell migration in Dictyostelium.

    abstract::A single cell of wild-type Dictyostelium discoideum forms a visible colony on a plastic dish in several days, but due to enhanced cell migration, amiB-null mutant cells scatter over a large area and do not form noticeable colonies. Here, with an aim to identify genes involved in cell migration, we isolated suppresser ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Nagasaki A,Uyeda TQ

    更新日期:2008-03-10 00:00:00

  • Lymphoblastoid cell variant in contact-mediated cell spreading.

    abstract::We have isolated a clone of human lymphoblastoid cells that is capable of undergoing the phenomenon of contact-mediated cell spreading in vitro. We have detected this behavior when using both transmission electron microscopy (TEM), and differential interference contrast microscopy. Upon cell-cell contact, cells become...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Sorrentino JA,Millis AJ,Whipple AP,Colquhoun WR

    更新日期:1983-05-01 00:00:00

  • Increased activity of chondroitin sulfate-synthesizing enzymes during proliferation of arterial smooth muscle cells.

    abstract::Cultured arterial smooth muscle cells incorporate [35S]sulfate into the extracellular chondroitin sulfate/dermatan sulfate containing proteoglycans at a higher rate in the phase of logarithmic growth than do non-dividing cells. The cell growth-dependent decrease in 35S incorporation with increasing cell density is acc...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hollmann J,Thiel J,Schmidt A,Buddecke E

    更新日期:1986-12-01 00:00:00

  • Role of the F-actin cytoskeleton in the RVD and RVI processes in Ehrlich ascites tumor cells.

    abstract::The role of the F-actin cytoskeleton in cell volume regulation was studied in Ehrlich ascites tumor cells, using a quantitative rhodamine-phalloidin assay, confocal laser scanning microscopy, and electronic cell sizing. A hypotonic challenge (160 mOsm) was associated with a decrease in cellular F-actin content at 1 an...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Pedersen SF,Mills JW,Hoffmann EK

    更新日期:1999-10-10 00:00:00

  • p75NTR enhances PC12 cell tumor growth by a non-receptor mechanism involving downregulation of cyclin D2.

    abstract::p75NTR is a member of the tumor necrosis superfamily of proteins which is variably associated with induction of apoptosis and proliferation. Cyclin D2 is one of the mediators of cellular progression through G1 phase of the cell cycle. The present study demonstrates the inverse relationship between expression of cyclin...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Fritz MD,Mirnics ZK,Nylander KD,Schor NF

    更新日期:2006-10-15 00:00:00

  • 6-Dimethylaminopurine blocks starfish oocyte maturation by inhibiting a relevant protein kinase activity.

    abstract::The puromycin analog N6,N6-dimethyladenine (6-dimethylaminopurine or 6-DMAP) was found to inhibit meiosis reinitiation in starfish oocytes stimulated by the natural hormone 1-methyladenine. Increasing concentrations of this agent delayed and eventually blocked germinal vesicle breakdown. They were found to be effectiv...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Neant I,Guerrier P

    更新日期:1988-05-01 00:00:00

  • Functional expression of human DNA topoisomerase I and its subcellular localization in HeLa cells.

    abstract::DNA topoisomerase (topo) I plays an important role in DNA metabolism by relieving the torsional restraints of DNA topology through ATP-independent single-strand DNA breakage. In the present study, we expressed human topo I in HeLa cells by fusing it to enhanced green fluorescent protein (EGFP). The EGFP-topo I fusion ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Mo YY,Wang P,Beck WT

    更新日期:2000-05-01 00:00:00

  • Integrity of actin fibers and microtubules influences metastatic tumor cell adhesion.

    abstract::Tumor cell adhesion within host organ microvasculature, its stabilization and invasion into host organ parenchyma appear to be important steps during formation of distant metastasis. These interactions of circulating tumor cells with the host organs occur in the presence of fluid shear forces and soluble and cellular ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Korb T,Schlüter K,Enns A,Spiegel HU,Senninger N,Nicolson GL,Haier J

    更新日期:2004-09-10 00:00:00

  • Some factors affecting the action of restriction endonucleases on human metaphase chromosomes.

    abstract::We have investigated whether restriction endonucleases produce bands on human chromosomes by extracting DNA, using staining methods which are stoichiometric for DNA. Restriction enzymes that produce C-band patterns appear to remove DNA extensively from chromosome arms. In general, however, those restriction enzymes th...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Mezzanotte R,Ferrucci L,Vanni R,Sumner AT

    更新日期:1985-11-01 00:00:00

  • Steroid hormone-induced changes in secreted proteins in the filamentous fungus Achlya.

    abstract::In the fungus Achlya, sexual development in the male strain E87 is mediated by the steroid hormone antheridiol. Treatment of vegetatively growing cells of E87 with antheridiol resulted in changes in the [35S]methionine labeling of several secreted proteins. The most heavily labeled group of proteins observed in the se...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Brunt SA,Silver JC

    更新日期:1986-03-01 00:00:00

  • Disruption of cytoplasmic microtubules by ultraviolet radiation.

    abstract::Ultraviolet (UV) irradiation of cultured human skin fibroblasts causes the disassembly of their microtubules. Using indirect immunofluorescence microscopy, we have now investigated whether damage to the microtubule precursor pool may contribute to the disruption of microtubules. Exposure to polychromatic UV radiation ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zamansky GB,Perrino BA,Chou IN

    更新日期:1991-07-01 00:00:00

  • Induction of beta 1 integrin synthesis by recombinant platelet-derived growth factor (PDGF-AB) correlates with an enhanced migratory response of human dermal fibroblasts to various extracellular matrix proteins.

    abstract::Cell migration plays a major role during wound healing and is tightly controlled by a variety of growth factors and extracellular matrix proteins. The experiments reported here have been designed to study whether defined beta 1 integrins are involved in the platelet-derived growth-factor-AB (PDGF-AB)-modulated migrato...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Kirchberg K,Lange TS,Klein EC,Jungtäubl H,Heinen G,Meyer-Ingold W,Scharffetter-Kochanek K

    更新日期:1995-09-01 00:00:00

  • Demonstration of a 7-nm RNP fiber as the basic structural element in a premessenger RNP particle.

    abstract::Balbiani ring granules in Chironomus salivary glands represent premessenger ribonucleoprotein (RNP) particles, each containing a 35- to 40-kb message for a secretory polypeptide. Their gross structure can be described as an RNP ribbon bent into a toroid. We now demonstrate that an unfolded, thin RNP fiber is observed ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lönnroth A,Alexciev K,Mehlin H,Wurtz T,Skoglund U,Daneholt B

    更新日期:1992-04-01 00:00:00