Abstract:
:LIM domain proteins are important regulators of the growth, determination, and differentiation of cells. In this report, FHL3 (human four-and-a-half LIM-only protein 3) is shown to interact with human phosphatase CDC25B, a cell cycle regulator involved in the control of G2/M. We found that this interaction was specific to the CDC25B2 isoform. Deletion and point mutation studies indicated that the second LIM domain of FHL3 was essential for this interaction. FRET experiments in C2C12 cells showed that, although both proteins were colocated in the cytoplasm and the nucleus, they interacted only in the nucleus. Finally, we showed that FHL3 binding impaired neither CDC25B2 phosphatase activity nor its localization. Further work is now needed to elucidate the consequences of this interaction on myoblast fate decision and cycle control.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Mils V,Lee SM,Joly W,Hang EW,Baldin V,Waye MM,Ducommun B,Tsui SKdoi
10.1016/s0014-4827(03)00018-1keywords:
subject
Has Abstractpub_date
2003-04-15 00:00:00pages
99-106issue
1eissn
0014-4827issn
1090-2422pii
S0014482703000181journal_volume
285pub_type
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