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Characterization of Caedibacter endonucleobionts from the macronucleus of Paramecium caudatum and the identification of a mutant with blocked R-body synthesis.

Abstract：

:Cytology, DNA and host-symbiont relationships of x-like endosymbionts from Paramecium caudatum are described. The symbionts (Caedibacter caryophila, sp. nov.) live in the macronuclei of their hosts. They confer the killer trait upon their hosts and appear well adapted to their endonucleobiotic way of life. R bodies (proteinaceous ribbons associated with killing) are produced, but differ significantly from any of the four R-body classes previously described. C. caryophila and their R bodies were isolated. DNA was extracted from purified symbionts and used to demonstrate that one P. caudatum line harbors a natural mutant which is deficient in R-body production. Melting studies indicate a GC content of 34.6%. No sequence homology between the C. caryophila DNA and the coding sequence for type 51 R-body production was observed. C. caryophila is parasitic, causing the death of its hosts in starving cultures.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Schmidt HJ,Görtz HD,Pond FR,Quackenbush RL

doi

10.1016/0014-4827(88)90141-3

subject

Has Abstract

pub_date

1988-01-01 00:00:00

pages

49-57

issue

1

eissn

0014-4827

issn

1090-2422

pii

0014-4827(88)90141-3

journal_volume

174

pub_type

杂志文章

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