Is the initiation of macronuclear DNA synthesis in Euplotes dependent on micronuclear functions?

abstract：:The mitochondrion imports and processes the vast majority of the proteins that constitute its structural elements and metabolic pathways. To study mitochondrial precursor processing in the context of the cellular environment, we employed the baculovirus expression system to overexpress the prototypical precursor prote...

journal_title：Experimental cell research

authors： Wright G,Reichenbecher V

更新日期：1999-02-01 00:00:00

abstract：:Porcine brain microvascular endothelial cells (PBMEC) were isolated from fresh brains by several enzymatic digestion steps followed by a gradient centrifugation. Cells of the primary culture were transfected with pRNS-1, encoding for the small and large T-antigens of SV 40, by means of lipofection. After selection wit...

journal_title：Experimental cell research

authors： Teifel M,Friedl P

更新日期：1996-10-10 00:00:00

abstract：:Our previous studies have showed that tumor-derived autophagosomes (termed "DRibbles") induce B cell activation, resulting in antibody production and cytokine secretion. Unexpectedly, we found that unfractionated splenocytes produced a higher level of antibody and cytokine than that of purified B cells. In the current...

journal_title：Experimental cell research

authors： Zhou M,Li W,Wen Z,Sheng Y,Ren H,Dong H,Cao M,Hu HM,Wang LX

更新日期：2015-02-15 00:00:00

abstract：:The involvement of polyamines in cytokinesis has been examined in eggs of the amphibian X. laevis. Microinjection of spermine or spermidine into unfertilized eggs induced a shortening of the first division cycle and early formation of cleavage-like constrictions. Eggs were activated by injection and developed furrows ...

journal_title：Experimental cell research

authors： Grant NJ,Aimar C,Oriol-Audit C

更新日期：1984-02-01 00:00:00

abstract：:In the human lymphoblastic cell line KE 37, Northern blot analysis with cDNA probes for human regulatory subunits RII alpha RII beta of the cAMP-dependent protein kinase (A-kinase) type II and immunoblotting or immunoprecipitation studies with several antibodies directed against RII alpha and RII beta show that these ...

journal_title：Experimental cell research

authors： Keryer G,Rios RM,Landmark BF,Skalhegg B,Lohmann SM,Bornens M

更新日期：1993-02-01 00:00:00

abstract：:Mdg1/ERdj4, a mammalian chaperone that belongs to the HSP40 protein family, has been reported to be located in the endoplasmic reticulum (ER), is induced by ER stress, and protects ER stressed cells from apoptosis. Here we show that under normal physiological conditions, Mdg1/ERdj4 is expressed at various levels in th...

journal_title：Experimental cell research

authors： Berger BJ,Müller TS,Buschmann IR,Peters K,Kirsch M,Christ B,Pröls F

更新日期：2003-10-15 00:00:00

abstract：:We established a differentiation method for homogeneous α7 integrin-positive human skeletal muscle stem cell (α7(+)hSMSC)-derived osteoblast-like cells with bone morphogenetic protein (BMP)-2. To explore the early signaling cascade for osteoblastic differentiation, we examined the upregulation of autophagy-related gen...

journal_title：Experimental cell research

authors： Ozeki N,Mogi M,Hase N,Hiyama T,Yamaguchi H,Kawai R,Matsumoto T,Nakata K

更新日期：2016-09-10 00:00:00

abstract：:The long non-coding RNAs (lncRNAs) have gained much attention due to its essential roles in molecular regulation. As one of the classic lncRNAs, H19 is strongly expressed during embryogenesis and plays a crucial biological function during development. Mesenchymal stem cells (MSCs) are an ideal cell source for tissue e...

journal_title：Experimental cell research

authors： Liu Y,Li G,Zhang JF

更新日期：2017-11-15 00:00:00

abstract：:Recently, we isolated 4 distinct kinds of single chain antibody against human EGF receptor (EGFR) after screening the Keio phage display scFv library by using two methods of target-guided proximity labeling. In the current study, these monovalent scFv antibodies were converted to bivalent IgGs of humanized forms (hIgG...

journal_title：Experimental cell research

authors： Chang C,Takayanagi A,Yoshida T,Shimizu N

更新日期：2013-05-01 00:00:00

abstract：:SIRT6, with both deacetylase and ADP-ribosyltransferase activities, is predominantly expressed in the nucleus. It has been revealed that SIRT6 regulates various biological functions including metabolism, aging and stress resistance. This study aims to investigate the role of SIRT6 in vascular inflammation and it molec...

journal_title：Experimental cell research

authors： He Y,Xiao Y,Yang X,Li Y,Wang B,Yao F,Shang C,Jin Z,Wang W,Lin R

更新日期：2017-08-01 00:00:00

abstract：:Microsomal triglyceride transfer protein (MTP) is a heterodimeric complex consisting of a unique large 97-kDa protein and the multifunctional 58-kDa protein disulfide isomerase (PDI). It plays an essential role in the assembly of lipoproteins by shuttling lipids between phospholipid membranes. Based on cell fractionat...

journal_title：Experimental cell research

authors： Slight I,Bendayan M,Malo C,Delvin E,Lambert M,Levy E

更新日期：2004-10-15 00:00:00

abstract：:Lithium (Li+) has been used in the treatment of manic-depressive disorders for several decades. More recently, Li+ has been shown to affect the signaling pathway of various neurotransmitters and growth/neurotrophic factors. We examined the effect of Li+ on the survival of cerebellar granule neurons in culture. Treatme...

journal_title：Experimental cell research

authors： D'Mello SR,Anelli R,Calissano P

更新日期：1994-04-01 00:00:00

abstract：:The epidermal growth factor (EGF) receptor is well known as a mediator of mitogenic signaling and its tyrosine kinase activity has been suggested as a viable target in cancer chemotherapy. To explore the consequences of abolishing the kinase activity of this receptor, we have utilized a potent and specific inhibitor o...

journal_title：Experimental cell research

authors： Nelson JM,Fry DW

更新日期：1997-06-15 00:00:00

abstract：:In dog thyrocytes in primary culture, thyrotropin (TSH), acting through cyclic AMP, induces proliferation and differentiation expression, while tetradecanoylphorbol acetate (TPA) or epidermal growth factor (EGF) induces proliferation and dedifferentiation. In this work, we have investigated the regulation of mRNA expr...

journal_title：Experimental cell research

authors： Pirson I,Dumont JE

更新日期：1994-10-01 00:00:00

abstract：:Autophagy is a cellular pathway involved in protein and organelle degradation. It is relevant to many types of cellular homeostasis and human diseases. High level of glucose is known to inflict podocyte injury, but little is reported about the relationship between high concentrations of glucose and autophagy in these ...

journal_title：Experimental cell research

authors： Ma T,Zhu J,Chen X,Zha D,Singhal PC,Ding G

更新日期：2013-04-01 00:00:00

abstract：:The adaptor protein Shb has previously been shown to regulate apoptosis in response to cytokines and inhibitors of angiogenesis although the mechanisms governing these effects have remained obscure. We currently demonstrate interactions between Shb and c-Abl and that Shb regulates c-Abl kinase activity. The data sugge...

journal_title：Experimental cell research

authors： Hägerkvist R,Mokhtari D,Lindholm C,Farnebo F,Mostoslavsky G,Mulligan RC,Welsh N,Welsh M

更新日期：2007-01-15 00:00:00

abstract：:Nuclear pore complexes (NPCs) provide the only sites for macromolecular transport between nucleus and cytoplasm. The nucleoporin p62, a component of higher eukaryotic NPCs, is located at the central gated channel and involved in nuclear trafficking of various cargos. p62 is organized into an N-terminal segment that co...

journal_title：Experimental cell research

authors： Stochaj U,Bański P,Kodiha M,Matusiewicz N

更新日期：2006-08-01 00:00:00

abstract：:Filamin is a well-characterized actin-associated protein first isolated from chicken smooth muscle. Subsequently, this polypeptide and its nonmuscle homolog actin-binding protein have been shown to be expressed in avian muscle tissue, mammalian smooth muscle, mammalian macrophages and other blood cell types, as well a...

journal_title：Experimental cell research

authors： Brown KD,Binder LI

更新日期：1993-12-01 00:00:00

abstract：:We have previously demonstrated that transient blocking of DNA synthesis with drugs such as 1-beta-D-arabinofuranosylcytosine results in some segments of the chromosomal DNA being replicated more than once in a single cell cycle. One explanation for this phenomenon might be that it is due to a perturbation of the cell...

journal_title：Experimental cell research

authors： Woodcock DM,Crowther PJ,Allan G,Cooper IA

更新日期：1983-08-01 00:00:00

abstract：:The expressions of the protooncogenes c-jun and jun D have been investigated in dog thyrocytes in a primary culture whose proliferation is stimulated by three distinct intracellular signaling pathways (1) the thyrotropin (TSH) or forskolin-cyclic-AMP-mediated cascade; (2) the protein kinase C pathway activated by diac...

journal_title：Experimental cell research

authors： Reuse S,Pirson I,Dumont JE

更新日期：1991-10-01 00:00:00

abstract：:Replication protein A (RPA), a stable complex of three polypeptides, is the single-stranded DNA-binding protein essential for DNA replication in eukaryotic cells. Previous studies of the subcellular distribution and stability of the RPA heterotrimer during the mammalian cell cycle have produced conflicting results. He...

journal_title：Experimental cell research

authors： Dimitrova DS,Gilbert DM

更新日期：2000-02-01 00:00:00

abstract：:A cell culture model stimulating the genetic deficiency of glucocerebrosidase has been developed, utilizing macrophages and conduritol B epoxide (CBE), the specific irreversible inhibitor of the enzyme. Rat peritoneal macrophage glucocerebrosidase was completely inhibited when cells were treated with 10 microM CBE for...

journal_title：Experimental cell research

authors： Das PK,Murray GJ,Gal AE,Barranger JA

更新日期：1987-02-01 00:00:00

abstract：:We have studied the use of electrofusion to obtain hybridomas producing antigen-specific antibodies after immunization of murine lymphocytes in vitro. Under optimal conditions fusion frequencies of the order of magnitude of 10(-3) were obtained, which is approximately 80-fold higher than the mean value obtained with f...

journal_title：Experimental cell research

authors： van Duijn G,Langedijk JP,de Boer M,Tager JM

更新日期：1989-08-01 00:00:00

abstract：:Centrosomes, the main microtubule-organizing centers (MTOCs) in most animal cells, are important for many cellular activities such as assembly of the mitotic spindle, establishment of cell polarity, and cell movement. In nuclear transfer (NT), MTOCs that are located at the poles of the meiotic spindle are removed from...

journal_title：Experimental cell research

authors： Zhong ZS,Zhang G,Meng XQ,Zhang YL,Chen DY,Schatten H,Sun QY

更新日期：2005-05-15 00:00:00

abstract：:Seven continuous cell lines have been established in vitro from lethal embryos produced by the female sterile mutant mh 1182 of Drosophila melanogaster. Six lines show haploid metaphases. Karyotype analysis revealed a high level of aneuploid cells with frequent chromosome fragments. In three lines, haploid cells were ...

journal_title：Experimental cell research

authors： Debec A

更新日期：1984-03-01 00:00:00

abstract：:Colony stimulating factor-1 (CSF-1) and the CSF-1 receptor (the c-fms proto-oncogene product) are expressed during the proliferation of the L6 alpha 1 rat myogenic cell line and both are down-regulated during the differentiation to myotubes. Biologically active CSF-1 was shown to be secreted into the culture medium by...

journal_title：Experimental cell research

authors： Borycki AG,Smadja F,Stanley R,Leibovitch SA

更新日期：1995-05-01 00:00:00

abstract：:Polo-like kinases (Plks) are essential for progression through mitosis. The activity of these kinases peak during M phase and this activation has been attributed to phosphorylation. Kinases capable of activating Plks in vitro have been previously identified both in mammalian cells and in Xenopus laevis oocytes (SLK an...

journal_title：Experimental cell research

authors： Alves PS,Godinho SA,Tavares AA

更新日期：2006-02-01 00:00:00

abstract：:Insulin-like growth factor-1 (IGF-1) and its receptor (IGF-1R) are required for cell proliferation in vitro, raising the possibility that an autocrine IGF-1-IGF-1R system may be present in vivo and become activated in the viable ventricular myocytes shortly after infarction. Therefore, following the in vivo documentat...

journal_title：Experimental cell research

authors： Reiss K,Kajstura J,Zhang X,Li P,Szoke E,Olivetti G,Anversa P

更新日期：1994-08-01 00:00:00

abstract：:Homologous desensitization of responses to bombesin in small cell lung cancer cells can be mimicked by protein kinase C-activating phorbol esters. We show that phorbol ester-induced desensitization can be blocked by prior treatment with the protein kinase C inhibitor staurosporine or prolonged pretreatment with phorbo...

journal_title：Experimental cell research

authors： Frankel A,Viallet J

更新日期：1993-12-01 00:00:00

abstract：:A heterogeneous RNP structure has been isolated from rat liver nuclei by a method previously used for the isolation of 30S RNP complexes carrying heterogeneous RNA (hnRNA) [1]. The RNP sediments in sucrose gradients with s-values of 70-110S. Formaldehyde-fixed preparations band at Q = 1.40 in isopycnic CsCl gradients....

journal_title：Experimental cell research

authors： Hatzoglou M,Adamtziki E,Margaritis L,Sekeris CE

更新日期：1985-03-01 00:00:00