Foxa1 contributes to the repression of Nanog expression by recruiting Grg3 during the differentiation of pluripotent P19 embryonal carcinoma cells.

abstract：:The Chinese hamster dihydrofolate reductase (DHFR) origin consists of many inefficient initiation sites scattered throughout a 55-kb intergenic spacer, with the ori-beta, ori-beta', and ori-gamma subregions being preferred. To test for the presence of genetic replicators within these subregions, fragments containing o...

journal_title：Experimental cell research

authors： Lin HB,Dijkwel PA,Hamlin JL

更新日期：2005-08-01 00:00:00

abstract：:By a sequential mutation and selection utilizing N-methyl-N'-nitro-N-nitrosoguanidine as a mutagen, we succeeded in separating a poly(ADP ribose) polymerase-defective mutant clone (Cl-3527) from a mouse L1210 cell clone (Cl-3). The enzyme activity per cell in Cl-3527 cells was only 8% of that in wild type L1210 (CCL 2...

journal_title：Experimental cell research

authors： Yoshihara K,Itaya A,Hironaka T,Sakuramoto S,Tanaka Y,Tsuyuki M,Inada Y,Kamiya T,Ohnishi K,Honma M

更新日期：1992-05-01 00:00:00

abstract：:The differential activation of cyclin and cyclin-dependent kinase genes by the adenovirus E1A gene product (E1A) or serum factors was studied with a rat 3Y1 derivative cell line, g12-21, in which the E1A12S cDNA can be expressed in response to dexamethasone (dex). The induction of DNA synthesis in quiescent g12-21 cel...

journal_title：Experimental cell research

authors： Ishii T,Shimizu M,Kanayama Y,Nakada S,Nojima H,Oda K

更新日期：1993-10-01 00:00:00

abstract：:SV40-transformation as well as treatment with tumor promoters produce alterations in morphology, differentiation and keratinization of human keratinocytes. Two cell lines of SV40-transformed keratinocytes and primary cultures of keratinocytes treated with the tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA) ...

journal_title：Experimental cell research

authors： Darmon M,Delescluse C,Semat A,Bernard B,Bailly J,Prunieras M

更新日期：1984-09-01 00:00:00

abstract：:LC3, a mammalian homologue of yeast Atg8, is assumed to play an important part in bulk sequestration and degradation of cytoplasm (macroautophagy), and is widely used as an indicator of this process. To critically examine its role, we followed the autophagic flux of LC3 in rat hepatocytes during conditions of maximal ...

journal_title：Experimental cell research

authors： Szalai P,Hagen LK,Sætre F,Luhr M,Sponheim M,Øverbye A,Mills IG,Seglen PO,Engedal N

更新日期：2015-04-10 00:00:00

abstract：:The lack of available, well characterized, established, domestic porcine cell lines hinders the advancement of porcine cellular immunology. A case of multicentric lymphoma was diagnosed in a market weight pig at the time of slaughter. Affected lymph nodes and spleen were collected and used for single cell isolation an...

journal_title：Experimental cell research

authors： Rahe MC,Dvorak CMT,Wiseman B,Martin D,Murtaugh MP

更新日期：2020-05-15 00:00:00

abstract：:Stromal-vascular cells from the epididymal fat pad of 4-week-old rats, when cultured in a medium containing insulin or insulin-like growth factor, IGF-I, triiodothyronine and transferrin, were able to undergo adipose conversion. Over ninety percent of the cells accumulated lipid droplets and this proportion was reduce...

journal_title：Experimental cell research

authors： Deslex S,Negrel R,Ailhaud G

更新日期：1987-01-01 00:00:00

abstract：:Mitotic PtK1 cells were treated at furrow initiation with microtubule poisons to determine the role of microtubules in the regulation of terminal cytokinetic events. The administration of anti-microtubule agents in late anaphase accelerated the rate of cytokinesis by approx. 60% as measured by changes in furrow diamet...

journal_title：Experimental cell research

authors： Hamilton BT,Snyder JA

更新日期：1983-04-01 00:00:00

abstract：:Partial outlet obstruction of the rabbit bladder induces serosal thickening and smooth muscle (SM) hypertrophy. Within thickened serosa, submesothelial (mesenchymal) cells differentiate into SM cells after 30 days of obstruction[S. Buoro et al. Lab. Invest. 69, 589-602, 1993]. Here, we show that submesothelial cells t...

journal_title：Experimental cell research

authors： Pampinella F,Roelofs M,Castellucci E,Chiavegato A,Guidolin D,Passerini-Glazel G,Pagano F,Sartore S

更新日期：1996-03-15 00:00:00

abstract：:The abundant heterogeneous nuclear ribonucleoprotein M (hnRNP M) is able to associate with early spliceosomes and to influence splicing patterns of specific pre-mRNAs. Here, by a combination of immunoprecipitation and pull-down assays, we have identified PSF (polypyrimidine tract-binding protein-associated splicing fa...

journal_title：Experimental cell research

authors： Marko M,Leichter M,Patrinou-Georgoula M,Guialis A

更新日期：2010-02-01 00:00:00

abstract：:Poly (ADP-ribose) polymerase-1 (PARP1) is a DNA damage sensor that gets activated in proportion to the damage, helping cells to determine whether to repair the damage or initiate cell death processes. We have previously shown PARP1's significance in the developmental processes of Dictyostelium discoideum in addition t...

journal_title：Experimental cell research

authors： Mir H,Rajawat J,Vohra I,Vaishnav J,Kadam A,Begum R

更新日期：2020-12-15 00:00:00

abstract：:Src-family kinases, cytoplasmic enzymes that participate in various signaling events, are found at not only the plasma membrane but also subcellular compartments, such as the nucleus, the Golgi apparatus and late endosomes/lysosomes. Lyn, a member of the Src-family kinases, is known to play a role in DNA damage respon...

journal_title：Experimental cell research

authors： Ikeda K,Nakayama Y,Togashi Y,Obata Y,Kuga T,Kasahara K,Fukumoto Y,Yamaguchi N

更新日期：2008-11-01 00:00:00

abstract：:Numerous factors that promote movement of macromolecules in and out of the nucleus have now been identified. These include both soluble cytoplasmic and nucleoplasmic proteins and proteins of the nuclear pore complex (NPC). Genetic analyses of the nuclear transport process in the model organism, the budding yeast Sacch...

journal_title：Experimental cell research

authors： Corbett A,Ferrigno P,Henry M,Kahana J,Koepp D,Lee M,Nguyen L,Schlenstedt G,Seedorf M,Shen E,Taura T,Wong D,Silver P

更新日期：1996-12-15 00:00:00

abstract：:Despite over 30 years of work, the fundamental structure of eukaryotic chromatin remains controversial. Here, we review the roots of this controversy in disparities between results derived from studies of chromatin in nuclei, chromatin isolated from nuclei, and chromatin reconstituted from defined components. Thanks t...

journal_title：Experimental cell research

authors： Grigoryev SA,Woodcock CL

更新日期：2012-07-15 00:00:00

abstract：:Tunneling nanotubes (TnTs) are long, non-adherent, actin-based cellular extensions that act as conduits for transport of cellular cargo between connected cells. The mechanisms of nanotube formation and the effects of the tumor microenvironment and cellular signals on TnT formation are unknown. In the present study, we...

journal_title：Experimental cell research

authors： Thayanithy V,Babatunde V,Dickson EL,Wong P,Oh S,Ke X,Barlas A,Fujisawa S,Romin Y,Moreira AL,Downey RJ,Steer CJ,Subramanian S,Manova-Todorova K,Moore MAS,Lou E

更新日期：2014-04-15 00:00:00

abstract：:Identification of new drugs and predicting drug response are major challenges in oncology, especially for brain tumors, because total surgical resection is difficult and radiation therapy or chemotherapy is often ineffective. With the aim of developing a culture system close to in vivo conditions for testing new drugs...

journal_title：Experimental cell research

authors： Jiguet Jiglaire C,Baeza-Kallee N,Denicolaï E,Barets D,Metellus P,Padovani L,Chinot O,Figarella-Branger D,Fernandez C

更新日期：2014-02-15 00:00:00

abstract：:Small vertebrate organisms have emerged as key players in the post-genomic era for the functional characterization of novel genes on a high-throughput scale. In this context, the zebrafish embryos and Xenopus tadpoles represent attractive and valuable models to rapidly identify and characterize novel genes involved in...

journal_title：Experimental cell research

authors： Ny A,Autiero M,Carmeliet P

更新日期：2006-03-10 00:00:00

abstract：:The von Hippel-Lindau (VHL) protein protects microtubules (MTs) from destabilization by nocodazole treatment. Based on this fixed-cell assay with static end points, VHL has been reported to directly stabilize the MT cytoskeleton. To investigate the dynamic changes in MTs induced by VHL in living cells, we measured the...

journal_title：Experimental cell research

authors： Lolkema MP,Mehra N,Jorna AS,van Beest M,Giles RH,Voest EE

更新日期：2004-12-10 00:00:00

abstract：:The Golgi apparatus undergoes extensive fragmentation during mitosis in animal cells. Protein kinases play a critical role during fragmentation of the Golgi apparatus. We reported here that Polo-like kinase 3 (Plk3) may be an important mediator during Golgi breakdown. Specifically, Plk3 was concentrated at the Golgi a...

journal_title：Experimental cell research

authors： Ruan Q,Wang Q,Xie S,Fang Y,Darzynkiewicz Z,Guan K,Jhanwar-Uniyal M,Dai W

更新日期：2004-03-10 00:00:00

abstract：:The p70 ribosomal S6 kinase (S6K1) is rapidly activated following growth factor stimulation of quiescent fibroblasts and inhibition of this enzyme results in a G(1) arrest. Phosphorylation of the ribosomal S6 protein by S6K1 regulates the translation of both ribosomal proteins and initiation factors, leading to an inc...

journal_title：Experimental cell research

authors： Zhang H,Hoff H,Marinucci T,Cristofalo VJ,Sell C

更新日期：2000-08-25 00:00:00

abstract：:Our previous studies have showed that tumor-derived autophagosomes (termed "DRibbles") induce B cell activation, resulting in antibody production and cytokine secretion. Unexpectedly, we found that unfractionated splenocytes produced a higher level of antibody and cytokine than that of purified B cells. In the current...

journal_title：Experimental cell research

authors： Zhou M,Li W,Wen Z,Sheng Y,Ren H,Dong H,Cao M,Hu HM,Wang LX

更新日期：2015-02-15 00:00:00

abstract：:Inhibition of cAMP-dependent protein kinase activity by microinjection of a specific physiologic protein inhibitor into sea urchin eggs inhibits the first cleavage after fertilization. Inhibition apparently occurs at some time prior to or during formation of the mitotic spindle. Measurement of the total protein kinase...

journal_title：Experimental cell research

authors： Browne CL,Bower WA,Palazzo RE,Rebhun LI

更新日期：1990-05-01 00:00:00

abstract：:Using hybridization in situ with a ribo-probe recognizing transcripts of the chicken alpha A globin gene, we show here that in proliferating AEV-transformed erythroblasts this gene is strongly transcribed, but the corresponding transcripts are retained in the nuclei. Most surprisingly, this globin RNA accumulates in t...

journal_title：Experimental cell research

authors： Iarovaia O,Razin SV,Linares-Cruz G,Sjakste N,Scherrer K

更新日期：2001-11-01 00:00:00

abstract：:Under standard culture conditions, normal human mammary epithelial cells (HMECs) divide a limited number of times before proliferation ceases in a growth-arrested state referred to as selection. Cells that have undergone spontaneous loss of p16(INK4a) expression due to hypermethylation of the p16(INK4a) CpG island eme...

journal_title：Experimental cell research

authors： Huschtscha LI,Neumann AA,Noble JR,Reddel RR

更新日期：2001-04-15 00:00:00

abstract：:Time-lapse microcinematography was used to trace the migration and subsequent fate of daughter pairs from single myogenic stem cells. The generation times of siblings which divide are closely correlated (r, 0.73) compared with randomly selected cells (r, 0.059), suggesting the more or less equal partition of stem cell...

journal_title：Experimental cell research

authors： Konigsberg IR,Pfister KK

更新日期：1986-11-01 00:00:00

abstract：:Claudins belong to a large family of transmembrane proteins that localize at tight junctions (TJs) where they play a central role in regulating paracellular transport of solutes and nutrients across epithelial monolayers. Their ability to regulate the paracellular pathway is highly influenced by changes in extracellul...

journal_title：Experimental cell research

authors： Lim TS,Vedula SR,Hui S,Kausalya PJ,Hunziker W,Lim CT

更新日期：2008-08-15 00:00:00

abstract：:Polycystins 1 and 2, which are mutated in Autosomal Polycystic Kidney Disease, are involved in mechanotransduction through various mechanisms. In renal cells, polycystins not only have an important mechanotransductive role in primary cilia but are also present in intercellular contacts but their role there remains unc...

journal_title：Experimental cell research

authors： Bhoonderowa L,Hameurlaine F,Arbabian A,Faqir F,Amblard F,Coscoy S

更新日期：2016-10-15 00:00:00

abstract：:The phenotypic transition of smooth muscle cells (SMC) from a contractile to a synthetic state appears to be an early event in the pathogenesis of atherosclerosis. We examined the effects of extracellular matrix components on the phenotypic modulation of rabbit arterial SMC in primary culture by flow cytometry. The re...

journal_title：Experimental cell research

authors： Yamamoto M,Yamamoto K,Noumura T

更新日期：1993-01-01 00:00:00

abstract：:Experiments are described which show that the torsional oscillations arising in a strand of the slime mould, Physarum polycephalum, under certain conditions remain closely correlated to oscillations in cytoplasmic streaming. When this is the case, the direction of rotation is related to the direction of streaming by a...

journal_title：Experimental cell research

authors： Obendorf P,O'Sullivan RA

更新日期：1983-11-01 00:00:00

abstract：:Dictyostelium discoideum cells synthesize and secrete the chemoattractant cAMP within minutes after chemotactic stimulation. During development, this signal-relay process is instrumental in cell aggregation, pattern formation, and differentiation. Cyclic AMP is known to accumulate inside the cell before secretion. In ...

journal_title：Experimental cell research

authors： Schoen CD,Arents JC,Bruin T,Van Driel R

更新日期：1989-03-01 00:00:00