Abstract：
:Using natural inducers of beta-glucuronidase, methyl-glucuronide and fructuronate, under gratuitous conditions (without metabolic conversion of these two compounds), we corroborate the fact that both molecules are required simultaneously in order to derepress the enzyme synthesis to a maximum level. Structurally related analogs of the natural inducers, thiophenyl-glucuronide and mannonic amide respectively, were assayed in the wild type and suitable mutant strains of E. coli. The results are in agreement with the model where the dual negative regulation of the enzyme synthesis is exerted by two regulatory genes uidR and uxuR. The concerted action of mannonic amide and thiophenyl-glucuronide, which alone fail to induce significantly beta-glucuronidase synthesis, reveals that a cooperative effect of the two repressor molecules responsible for the complete blocking of the enzyme synthesis is occuring.
journal_name
Biochimiejournal_title
Biochimieauthors
Mandrand-Berthelot MA,Novel G,Novel Mdoi
10.1016/s0300-9084(77)80287-3subject
Has Abstractpub_date
1977-01-01 00:00:00pages
163-70issue
2eissn
0300-9084issn
1638-6183pii
S0300-9084(77)80287-3journal_volume
59pub_type
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