Abstract：
:Stereospecific hydrogenations according to the general scheme [formula: see text] are of interest from a preparative and mechanistic point of view. Proteus mirabilis is suitable for the hydrogenation of a-keto-acids to R-hydroxy-acids, and a Clostridium strain for the reduction of enoates. Both have been immobilized in formaldehyde crosslinked gelatin and the latter also in polyacrylamide. Immobilized as well as free cells showed usually half lives of 100-200 h. The immobilized cells could be separated from the products and reused. In order to hydrogenate enoates stereospecifically, formate dehydrogenase and enoate reductase have been separately immobilized and coimmobilized on controlled pore glass. The yields for the separately immobilized enzymes were about 30 per cent and 70-80 per cent, respectively. The measured rate of the coupled system with immobilized enzymes was compared with the calculated rate, taking into account effects of pore diffusion for the pyridine nucleotide. Under operational conditions the half-life of the immobilized formate dehydrogenase was 36 h versus 45 h for the free enzyme. The corresponding values for the enoate reductase turned out to be about 17 h versus about 15 h. So far the immobilized as well as the free enzymes seem to be less stable than immobilized or free cells.
journal_name
Biochimiejournal_title
Biochimieauthors
Tischer W,Tiemeyer W,Simon Hdoi
10.1016/s0300-9084(80)80162-3subject
Has Abstractpub_date
1980-01-01 00:00:00pages
331-9issue
5-6eissn
0300-9084issn
1638-6183pii
S0300-9084(80)80162-3journal_volume
62pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:Evidence from clinical, animal and cell culture studies demonstrates that increased autotaxin (ATX) expression is responsible for enhancing tumor progression, cell migration, metastases, angiogenesis and chemo-resistance. These effects depend mainly on the rapid formation of lysophosphatidate (LPA) by ATX. Circulating...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2010.08.002
更新日期：2011-01-01 00:00:00
abstract：:The kinetoplastid protozoan Leishmania encodes major surface glycoprotein GP63, a zinc metallo-peptidase (EC.3.4.24.36) expressed both in promastigote and amastigote life stages. In the present study, we explored for the first time the role of C-terminal domain (CTD) in proteinase activity by serial truncation of Leis...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.07.014
更新日期：2010-12-01 00:00:00
abstract：:We have analysed the complete sequence of the Escherichia coli K12 isolate MG1655 genome for chromatin-associated protein binding sites, and compared the predicted location of predicted sites with experimental expression data from 'DNA chip' experiments. Of the dozen proteins associated with chromatin in E. coli, only...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(00)01225-6
更新日期：2001-02-01 00:00:00
abstract：:GcvB is a non-coding RNA that regulates oppA mRNA in different bacterial species by binding a GcvB GU-rich region named R1 to oppA mRNA. A secondary putative interaction site (PS1) was identified in this study that is able to form a second nearly perfect 10 base-pair duplex between these two RNAs in Escherichia coli. ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.06.020
更新日期：2010-10-01 00:00:00
abstract：:Human Rad51 (HsRad51), a key element of the homologous recombination repair pathway, is related to the resistance of cancer cells to chemo- and radio-therapies. This protein is thus a good target for the development of anti-cancer treatments. We have searched for new inhibitors directed against HsRad51 using the Syste...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.08.006
更新日期：2010-12-01 00:00:00
abstract：:A variety of species of GPDH undergo acylation at two of the four active cystein sites per mole of tetrameric enzyme. This reaction requires tightly bound NAD+, a situation restricted to two of the four NAD sites per tetramer. S leads to N acyl transfer from cysteins to lysine in the diacyl enzyme yields an inactive e...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(81)80177-0
更新日期：1981-02-01 00:00:00
abstract：:This review article outlines some potentials and limits of the recent application of high resolution Nuclear Magnetic Resonance technique--coupled to the Fourier transformation methods--to the study of biological membranes. Molecular arrangement and dynamical structure characters can be assessed at the level of indivi...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(75)80333-6
更新日期：1975-01-01 00:00:00
abstract：:Gallium, a group IIIa metal salt, has been demonstrated to be an effective immunosuppressive agent. Gallium has also been shown to inhibit the production of inflammatory cytokines, such as IL-1beta, produced by macrophage-like cells in vitro. To further characterize the effects of gallium on the inflammatory process, ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(00)00384-9
更新日期：2000-02-01 00:00:00
abstract：:In all living cells 3' ends of RNA are posttranscriptionally elongated or shortened by nucleotidyl transferases and ribonucleases. The detailed analysis of the rpsO mRNA of Escherichia coli presented here demonstrates that transcription terminates in vivo at two sites located seven and eight nucleotides downstream fro...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2005.02.012
更新日期：2005-09-01 00:00:00
abstract：:Several inositol isomers and in particular myo-inositol (MI) and D-chiro-inositol (DCI), were shown to possess insulin-mimetic properties and to be efficient in lowering post-prandial blood glucose. In addition, abnormalities in inositol metabolism are associated with insulin resistance and with long term microvascula...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2013.05.011
更新日期：2013-10-01 00:00:00
abstract：:The family of interferon regulatory transcription factors (IRF) participates in the virus-induced and dsRNA-stimulated transcriptional regulation of either type I IFN genes or a definite set of genes which can also be activated by IFN. In this review, we place emphasis on the role of IRF-3 that associates with the coa...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(99)80021-2
更新日期：1998-08-01 00:00:00
abstract：:Human prophospholipase A2 was purified from pancreatic juice. The protein has a molecular weight of 14500 and a free N-terminal residue identified as aspartic acid (or asparagine). The amino acid composition was determined. Partial immunological identity has been obtained between human and porcine prophospholipase A2....
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(81)80216-7
更新日期：1981-08-01 00:00:00
abstract：:Innate immune system is the first line of host defense against invading microorganisms. It relies on a limited number of germline-encoded pattern recognition receptors that recognize conserved molecular structures of microbes, referred to as pathogen-/microbe-associated molecular patterns (PAMPs/MAMPs). Bacterial cell...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2012.06.007
更新日期：2013-01-01 00:00:00
abstract：:After 30 years of effort, the mammalian adenylate cyclase catalytic subunit has now been purified. It is a glycoprotein of 155 kDa, representing less than 0.01% of synaptosomal membrane protein. As measured in the presence of forskolin, its specific activity is 10-20 mumol of cAMP X mg-1 X min-1. The enzyme obtained i...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(87)90016-2
更新日期：1987-04-01 00:00:00
abstract：:Canine S100A12 (cS100A12) is a calcium-binding protein of the S100 superfamily of EF-hand proteins, and its expression is restricted to neutrophils and monocytes. Interaction of S100A12 with the receptor for advanced glycation end products (RAGE) has been suggested to play a central role in inflammation. Moreover, S10...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.08.007
更新日期：2010-12-01 00:00:00
abstract：:Wheat germ lysate was used as a model system for in vitro translation. We show that an increase of the exchange surface between the reaction mixture and the atmosphere enhanced the amount of incorporated cysteine, indicating that early arrest of protein synthesis previously observed in such a system was due to oxygen ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(96)88123-5
更新日期：1995-01-01 00:00:00
abstract：:A series of halomethylated derivatives of dihydrocoumarins has been found to inhibit irreversibly proteases and esterases. alpha-Chymotrypsin, subtilish, elastase are rapidly inactivated in the presence of these compounds, while trypsin, kallicrein, papain are inhibited more slowly. Esterases like acetylcholinesterase...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(77)80140-5
更新日期：1977-01-01 00:00:00
abstract：:Homeostasis in vivo is maintained by a highly complex network of positive and negative signals. At the cellular level, this regulatory microenvironment can be divided, in a simplified fashion, into two major compartments: the humoral compartment, including compounds such as hormones, growth factors and nutrients, and ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(88)90301-x
更新日期：1988-11-01 00:00:00
abstract：:The effects of DNA oligonucleotides complementary to potato spindle tuber viroid (PSTVd) on viroid infection were investigated. The oligonucleotides were used to form hybrids with PSTVd in the infection mixture. A 75% reduction of viroid infection was found when an oligonucleotide complementary to nucleotides 79-110 o...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(93)90026-o
更新日期：1993-01-01 00:00:00
abstract：PURPOSE:We evaluated the clinical relevance of microRNA-149 (miR-149) in neuroblastoma (NB) and its functional roles in regulating NB proliferation in vitro. METHODS:QRT-PCR was used to evaluate miR-149 expression in NB cell lines and primary NB tumors. Association between endogenous miR-149 expression in primary NB t...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.04.011
更新日期：2017-08-01 00:00:00
abstract：:Synthesis and secretion of polysaccharides by Gram-negative bacteria are a result of a concerted action of enzymatic and channel-forming proteins localized in different compartments of the cell. The presented work comprises functional characterization of PssO protein encoded within the previously identified, chromosom...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2008.08.004
更新日期：2008-11-01 00:00:00
abstract：:For efficient DNA hydrolysis, Type III restriction endonuclease EcoP15I interacts with two inversely oriented recognition sites in an ATP-dependent process. EcoP15I consists of two methylation (Mod) subunits and a single restriction (Res) subunit yielding a multifunctional enzyme complex able to methylate or to hydrol...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2012.11.014
更新日期：2013-04-01 00:00:00
abstract：:A set of sequences can be defined by their common subsequences, and the length of these is a measure of the overall resemblance of the set. Each subsequence corresponds to a succession of symbols embedded in every sequence, following the same order but not necessarily contiguous. Determining the longest common subsequ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(85)80271-6
更新日期：1985-05-01 00:00:00
abstract：:A novel bgl1 gene, encoding GH3 family β-glucosidase from Penicillium verruculosum (PvBGL), was cloned and heterologously expressed in P. canescens RN3-11-7 (niaD-) strain under the control of the strong xylA gene promoter. The recombinant rPvBGL was purified and their properties were studied in comparison with those ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2019.11.009
更新日期：2020-01-01 00:00:00
abstract：:Ferritins are ubiquitous iron-storage proteins found in all kingdoms of life. They share a common architecture made of 24 subunits of five α-helices. The recombinant Chlorobium tepidum ferritin (rCtFtn) is a structurally interesting protein since sequence alignments with other ferritins show that this protein has a si...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2014.07.019
更新日期：2014-11-01 00:00:00
abstract：:Nucleolin (NCL) is one of the most abundant non ribosomal protein of the nucleolus where it plays a central role in polymerase I transcription. NCL is also found outside of the nucleolus, in the nucleoplasm, cytoplasm as well as on the cell membrane. It acts in all cell compartments to control cellular homeostasis and...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2015.03.023
更新日期：2015-06-01 00:00:00
abstract：:Embryonic stem cells (ESCs) are capable of unlimited self-renewal and differentiation into multiple cell types. Recent large-scale analyses have identified various cell surface molecules on ESCs. Some of them are considered to be beneficial markers for characterization of cellular phenotypes and/or play an essential r...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.10.017
更新日期：2012-01-01 00:00:00
abstract：:The methylotrophic yeast Pichia pastoris is often used as an organism for the heterologous expression of proteins and has been used already for production of a number of glycosyltransferases involved in the biosynthesis of N- and O-linked oligosaccharides. In our recent studies, we have examined the expression in P. p...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(03)00072-5
更新日期：2003-03-01 00:00:00
abstract：:The eukaryotic ribosomal protein S15 is a key component of the decoding site in contrast to its prokaryotic counterpart, S19p, which is located away from the mRNA binding track on the ribosome. Here, we determined the oligopeptide of S15 neighboring the A site mRNA codon on the human 80S ribosome with the use of mRNA ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.02.031
更新日期：2010-07-01 00:00:00
abstract：:NADH cytochrome b5 oxidoreductase (Ncb5or) protects β-cells against oxidative stress and lipotoxicity. The predominant phenotype of lean Ncb5or-null mouse is insulin-dependent diabetes due to β-cell death. This suggests the putative role of NCB5OR polymorphism in human diabetes. Therefore, we aimed to investigate the ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.03.004
更新日期：2013-07-01 00:00:00
