Regulation of CIRL-1 proteolysis and trafficking.

abstract：:Epithelial Ca(2+)-regulation, which governs cornified envelope formation in the skin epidermis and hair follicles, closely coincides with the expression of S100A3, filaggrin and trichohyalin, and the post-translational modification of these proteins by Ca(2+)-dependent peptidylarginine deiminases. This review summariz...

journal_title：Biochimie

authors： Kizawa K,Takahara H,Unno M,Heizmann CW

更新日期：2011-12-01 00:00:00

abstract：:In order to demonstrate the in vivo antioxidant properties of metallothioneins (MTs), the bacteria Escherichia coli was used as a cell reactor in which we compared the metal binding and antioxidative functions of MTs from different species, with different structures and polypeptide lengths. No protective effects of cy...

journal_title：Biochimie

authors： Achard-Joris M,Moreau JL,Lucas M,Baudrimont M,Mesmer-Dudons N,Gonzalez P,Boudou A,Bourdineaud JP

更新日期：2007-12-01 00:00:00

abstract：:An apparatus for chemi- and bioluminescence measurements is described. The main features are in high sensitivity: for example, 5 10(-12) M adenosine triphosphate, 5 10(-7) M glucose, 2 10(-9) M perhydrol and 4 10(-13) M peroxidase are easily detected; its fast mixing time and the possibility of injecting a reactant, a...

journal_title：Biochimie

authors： Arrio B,Lecuyer B,Dupaix A,Volfin P,Jousset M,Carrette A

更新日期：1980-01-01 00:00:00

abstract：:In the present study we have investigated the thermal stability of the globular transport protein human serum albumin (HSA), in the presence of two small chain polyethylene glycols (namely PEG 200 and PEG 400). Both near- and far-UV circular dichroism (CD) study reveal that addition of PEG moderately increases the α-h...

journal_title：Biochimie

authors： Samanta N,Mahanta DD,Hazra S,Kumar GS,Mitra RK

更新日期：2014-09-01 00:00:00

abstract：:Tri(n-butyl)phosphate (TNBP) and sodium cholate (SC) mixtures have been used to inactivate lipid-enveloped viruses like HIV and hepatitis B. We exploited the use of this combination to purify fibroblast growth factor-2 (FGF-2) from human placenta. Human placentas were extracted in the presence of 0.3% TNBP/0.2% SC and...

journal_title：Biochimie

authors： Wolff P,Tanaka AM,Chenker E,Cabrera-Crespo J,Raw I,Ho PL

更新日期：1996-01-01 00:00:00

abstract：:The nuclear metabolism of poly(ADP-ribose) is mainly regulated by poly(ADP-ribose) polymerase-1 (PARP-1) and by poly(ADP-ribose) glycohydrolase (PARG). A PARP-like enzyme, V-PARP, and a PARG isoform are present in the extra-nuclear compartment of mammalian cells, even if poly(ADP-ribose) has never been detected therei...

journal_title：Biochimie

authors： Rossi L,Denegri M,Torti M,Poirier GG,Ivana Scovassi A

更新日期：2002-12-01 00:00:00

abstract：:GABARAPL1 belongs to the small family of GABARAP proteins (including GABARAP, GABARAPL1 and GABARAPL2/GATE-16), one of the two subfamilies of the yeast Atg8 orthologue. GABARAPL1 is involved in the intracellular transport of receptors, via an interaction with tubulin and GABA(A) or kappa opioid receptors, and also par...

journal_title：Biochimie

authors： Seguin-Py S,Lucchi G,Croizier S,Chakrama FZ,Despouy G,Le Grand JN,Ducoroy P,Boireau W,Boyer-Guittaut M,Jouvenot M,Fraichard A,Delage-Mourroux R

更新日期：2012-03-01 00:00:00

abstract：:The efficiency of oxidative phosphorylation was estimated in intact resting cells of Escherichia coli K 12, strain PA 601 (chl-s) and its chl-r mutants, all of them grown anaerobically in the presence of nitrate. The oxidation of endogenous NADH in intact chl-s cells was accompanied by the formation of ATP whatever th...

journal_title：Biochimie

authors： Giordano G,Rivière C,Azoulay E

更新日期：1977-01-01 00:00:00

abstract：:RNA-protein crosslinks were introduced into the 40S ribosomal subunits from Saccharomyces cerevisiae by mild UV treatment. Proteins crosslinked to the 18S rRNA molecule were separated from free proteins by repeated extraction of the treated subunits and centrifugation in glycerol gradients. After digestion with RNase ...

journal_title：Biochimie

authors： Xiang RH,Lee JC

更新日期：1989-11-01 00:00:00

abstract：:The activation of the antitumor protein antibiotic neocarzinostatin (NCS) by the carboxyl radical CO-2, a one-electron donor obtained selectively from gamma-ray irradiation of nitrous oxide-saturated formate buffer, has been investigated in the presence and in the absence of DNA at pH 4.7 and pH 7.0. The reaction of N...

journal_title：Biochimie

authors： Favaudon V

更新日期：1983-11-01 00:00:00

abstract：:Phosphoenolpyruvate carboxykinase (PEPCK) is the key enzyme in glyceroneogenesis, an important metabolic pathway that functions to restrain the release of non-esterified fatty acids (NEFAs) from adipocytes. The antidiabetic drugs known as thiazolidinediones (TZDs) are thought to achieve some of their benefits by lower...

journal_title：Biochimie

authors： Glorian M,Duplus E,Beale EG,Scott DK,Granner DK,Forest C

更新日期：2001-10-01 00:00:00

abstract：:A d-carbamoylase from Sinorhizobium morelens S-5 was purified and characterized. The enzyme was purified 189-fold to homogeneity with a yield of 19.1% by aqueous two-phase extraction and two steps of column chromatography. The enzyme is a homotetramer with a native molecular mass of 150 kDa and a subunit relative mole...

journal_title：Biochimie

authors： Wu S,Liu Y,Zhao G,Wang J,Sun W

更新日期：2006-03-01 00:00:00

abstract：:Fluorometry using a substrate DNA labeled with a single fluorophore (6-carboxyfluorescein) at the 3'-end of the processed strand was shown to be a useful tool for monitoring DNA-binding and 3'-processing activities of HIV-1 and PFV integrases (INs). The DNA binding to either of the INs resulted in a fluorescence signa...

journal_title：Biochimie

authors： Anisenko A,Agapkina J,Zatsepin T,Yanvarev D,Gottikh M

更新日期：2012-11-01 00:00:00

abstract：:Purified chromatin isolated from lymphocytic cells derived from patients with acute leukemia, or other lymphoproliferative disorders has been compared with chromatin isolated from normal human lymphocytic cells by gel electrophoresis and differential gradient ultracentrifugation. Thermal denaturation studies showed hi...

journal_title：Biochimie

authors： Desai LS,Wulff UC,Foley GE

更新日期：1975-01-01 00:00:00

abstract：:Oxidative stress, characterized by the accumulation of reactive oxygen species (ROS), is known to have numerous detrimental effects on the myocardium such as the induction of apoptotic cell death, hypertrophy, fibrosis, dysfunction, and dilatation. Over the past several years, we have shown that mitochondrial NADP(+)-...

journal_title：Biochimie

authors： Lee JH,Park JW

更新日期：2014-04-01 00:00:00

abstract：:Three mutanase (alpha-1,3-glucanase)-producing microorganisms isolated from soil samples were identified as a relatives of Paenibacillus. A mutanase was purified to homogeneity from cultures of each, and the molecular masses of the purified enzymes were approximately 132, 141, and 141kDa, respectively. The correspondi...

journal_title：Biochimie

authors： Hakamada Y,Sumitomo N,Ogawa A,Kawano T,Saeki K,Ozaki K,Ito S,Kobayashi T

更新日期：2008-03-01 00:00:00

abstract：:The possibility that brown adipose tissue thermogenesis can be recruited in order to combat the development of obesity has led to a high interest in the identification of "browning agents", i.e. agents that increase the amount and activity of UCP1 in brown and brite/beige adipose tissues. However, functional analysis ...

journal_title：Biochimie

authors： Kalinovich AV,de Jong JM,Cannon B,Nedergaard J

更新日期：2017-03-01 00:00:00

abstract：:The maximum activities of some key enzymes of metabolism were studied in lungs of fed and 48-h-starved rats. The maximum activity of hexokinase in the lung is similar to that of other tissues of the body, but lower than that of phosphorylase and 6-phosphofructokinase. High activities of glucose-6-phosphate dehydrogena...

journal_title：Biochimie

authors： Ardawi MS

更新日期：1991-05-01 00:00:00

abstract：:Bikunin (Bk) is a Künitz-type serine proteinase inhibitor, which occurs in human plasma, mainly as covalent complexes with one or two of the three peptide heavy chains. The leading member of this glycoprotein family is inter-alpha-inhibitor (I alpha I), which consists of two heavy chains (H1 and H2) linked to Bk. Bk c...

journal_title：Biochimie

authors： Capon C,Mizon C,Lemoine J,Rodié-Talbère P,Mizon J

更新日期：2003-01-01 00:00:00

abstract：:Lactic acid bacteria provide a model system for the in vivo study of mechanisms pertaining to the regulation of sugar transport and metabolism by microorganisms. Recent studies with resting and growing cells of the homofermentative Streptococci and Lactobacilli have yielded evidence for hitherto unsuspected regulatory...

journal_title：Biochimie

authors： Thompson J

更新日期：1988-03-01 00:00:00

abstract：:Apoptosis is the physiological process by which unwanted cells in an organism are killed. Bcl-2, a membrane-bound cytoplasmic protein, and its close relative Bcl-xL, are both effective inhibitors of apoptosis induced by a wide variety of stimuli in many different cell types. In a previous study, we reported that suppr...

journal_title：Biochimie

authors： Cherbonnel-Lasserre C,Dosanjh MK

更新日期：1997-10-01 00:00:00

abstract：:A novel bgl1 gene, encoding GH3 family β-glucosidase from Penicillium verruculosum (PvBGL), was cloned and heterologously expressed in P. canescens RN3-11-7 (niaD-) strain under the control of the strong xylA gene promoter. The recombinant rPvBGL was purified and their properties were studied in comparison with those ...

journal_title：Biochimie

authors： Volkov PV,Rozhkova AM,Zorov IN,Sinitsyn AP

更新日期：2020-01-01 00:00:00

abstract：:DNA replication in Escherichia coli is controlled at the initiation stage, possibly by regulation of the essential activity of DnaA protein. The cellular membrane has long been hypothesized to be involved in chromosomal replication. Accumulating evidence, both in vitro and in vivo, that supports the importance of memb...

journal_title：Biochimie

authors： Crooke E

更新日期：2001-01-01 00:00:00

abstract：:Glycogen was described as a temporal storage molecule in rhodococci, interconnecting lipids and carbon availability. The Rhodococcus jostii ADP-glucose pyrophosphorylase (ADP-GlcPPase) kinetic and regulatory properties support this role. Curiously, the enzyme uses glucosamine-1P as alternative substrate. Herein, we re...

journal_title：Biochimie

authors： Cereijo AE,Alvarez HM,Iglesias AA,Asencion Diez MD

更新日期：2020-09-01 00:00:00

abstract：:Seven virulent bacteriophages of Streptococcus thermophilus were characterized at the molecular level and classified into 2 subgroups (A and B) by DNA/DNA hybridization experiments and analysis of their structural proteins. Two representatives of subgroups A and B were compared to 3 representatives of Neve's subgroups...

journal_title：Biochimie

authors： Benbadis L,Faelen M,Slos P,Fazel A,Mercenier A

更新日期：1990-12-01 00:00:00

abstract：:The complete amino acid sequences of 3 proteins from the 50S subunit of Bacillus stearothermophilus ribosomes were determined by N-terminal sequence analysis and by sequencing of overlapping fragments obtained from enzymatic digestions and chemical cleavages. The proteins BstL28, BstL33 and BstL34, named according to ...

journal_title：Biochimie

authors： Kruft V,Kapp U,Wittmann-Liebold B

更新日期：1991-07-01 00:00:00

abstract：:The misfolding of amyloid proteins is closely correlated with the pathogenesis of protein conformation-related diseases, such as Alzheimer's disease (AD), prion disease, and type 2 diabetes mellitus (T2DM). The deposition of human islet amyloid polypeptide (hIAPP) and amyloid-β (Aβ) protein is entangled in AD and diab...

journal_title：Biochimie

authors： Xu J,Zhao C,Huang X,Du W

更新日期：2020-07-01 00:00:00

abstract：:A novel approach to genetic sequence analysis is presented. This approach, based on compression of algorithms, has been launched simultaneously by Grumbach and Tahi, Milosavljevic and Rivals. To reduce the description of an object, a compression algorithm replaces some regularities in the description by special codes....

journal_title：Biochimie

authors： Rivals E,Dauchet M,Delahaye JP,Delgrange O

更新日期：1996-01-01 00:00:00

abstract：:Interferon-gamma is a key cytokine in tumor immunosurveillance. The recently described interferon-producing killer dendritic cell (IKDC), can be distinguished from other innate effectors by its ability to kill a large variety of tumor cells and to produce high amounts of interferon-gamma after encountering tumors in t...

journal_title：Biochimie

authors： Bonmort M,Ullrich E,Mignot G,Jacobs B,Chaput N,Zitvogel L

更新日期：2007-06-01 00:00:00

abstract：:The complete nucleotide sequence of a nerve growth factor precursor from Bothrops jararacussu snake (Bj-NGF) was determined by DNA sequencing of a clone from cDNA library prepared from the poly(A) + RNA of the venom gland of B. jararacussu. cDNA encoding Bj-NGF precursor contained 723 bp in length, which encoded a pre...

journal_title：Biochimie

authors： Kashima S,Soares AM,Roberto PG,Pereira JO,Astolfi-Filho S,Cintra AO,Fontes MR,Giglio JR,de Castro França S

更新日期：2002-07-01 00:00:00