Abstract：
:Total poly (A+) RNA has been extracted from rat pheochromocytoma and translated in vitro by means of a reticulocyte lysate system. We show that two antisera, prepared against pig kidney DOPA decarboxylase (DDC) or rat pheochromocytoma DDC, immunoprecipitate an in vitro synthetized 50 kDa polypeptide identified as DDC by competition experiments with pure DDC. The proportion of specific mRNA has been calculated and represents 0.05% of total poly A+ mRNA. Its size has been established by electrophoresis in methylmercuric hydroxide containing agarose gel, corresponding to a 2.2 kb length mRNA.
journal_name
Biochimiejournal_title
Biochimieauthors
Bruneau G,Krieger-Poullet M,Coge F,Borri-Voltattorni C,Gros F,Thibault Jdoi
10.1016/0300-9084(90)90175-gsubject
Has Abstractpub_date
1990-01-01 00:00:00pages
73-6issue
1eissn
0300-9084issn
1638-6183pii
0300-9084(90)90175-Gjournal_volume
72pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:Over-expression of efflux transporter P-glycoprotein (PgP) encoded by ABCB1 gene has been implicated in poor responsive epilepsy. Several genetic variants have been shown to influence the expression levels of P-glycoprotein. The aim of the present study was to investigate the role of ABCB1 polymorphisms: C1236T, G2677...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.04.008
更新日期：2010-09-01 00:00:00
abstract：:When ribosomes encounter mRNAs lacking stop codons, two quality-control machineries, NSD for nonstop mRNA decay and ribosome quality control (RQC) for co-translational degradation of the nonstop protein by the proteasome, are triggered to eliminate aberrant molecules. In yeast, it is known that Dom34 (a homolog of eRF...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2014.08.001
更新日期：2014-11-01 00:00:00
abstract：:Insulin stimulates protein synthesis in skeletal muscle of young animals and has been reported to exert similar effects on a variety of mammalian cell types in culture. However, with chick embryo fibroblasts we found that the extent of this effect was very sensitive to the culture conditions of the cells prior to the ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(93)90129-g
更新日期：1993-01-01 00:00:00
abstract：:Most evolutionists agree to consider that our present RNA/DNA/protein world has originated from a simpler world in which RNA played both the role of catalyst and genetic material. Recent findings from structural studies and comparative genomics now allow to get a clearer picture of this transition. These data suggest ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2005.03.015
更新日期：2005-09-01 00:00:00
abstract：:In order to study the relationship between bile cholesterol and free cholesterol carried by high and low density lipoproteins (HDL and LDL), 10 male Wistar rats, 11 weeks old and fed with a standard diet were divided into 3 groups which received an intravenous infusion (jugular vein) of either LDL, HDL or liposomes. L...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(87)90270-7
更新日期：1987-01-01 00:00:00
abstract：:Personalized and regenerative medicine is an emerging therapeutic strategy that is based on cell biology and biomedical engineering used to develop biological substitutes to maintain normal function or restore damaged tissues and organs. The secretory capacities of different cell types are now explored as such possibl...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2018.04.015
更新日期：2018-12-01 00:00:00
abstract：:L-aromatic aminoacid decarboxylase has been purified more than thousand times from homogenates of rat brain, in several steps : centrifugation, DEAE-cellulose, CM cellulose, hydroxylapatite, DEAE sephadex. Its properties have been studied, most of them on an intermediate fraction of the purification, because of the in...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(75)80356-7
更新日期：1975-01-01 00:00:00
abstract：:Knowing the structure of a protein is essential to characterize its function and mechanism at the molecular level. Despite major advances in solving structures experimentally, most membrane protein native conformations remain unknown. This lack of available structures, along with the physical constraints imposed by th...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2015.05.018
更新日期：2015-08-01 00:00:00
abstract：:A higher level of N-acetylglucosamine incorporation by proteinic and polyprenic endogenous acceptors is observed after infection by Myxovirus. This phenomenon occurs in whole mitochondria as in outer mitochondrial membranes, where it is particularly obvious with proteinic endogenous acceptors. Under viral infection, n...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(80)80373-7
更新日期：1980-01-01 00:00:00
abstract：:NDPK-A, NDPK-B and NDPK-D are three enzymes which belong to the NDPK group I isoforms and are not only involved in metabolism process but also in transcriptional regulation, DNA cleavage, histidine protein kinase activity and metastasis development. Those enzymes were reported to bind to membranes either in mitochondr...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2014.06.025
更新日期：2014-10-01 00:00:00
abstract：:Rhodospirillum salexigens is a moderately halophilic purple phototrophic bacterium which grows optimally in 8% NaCl. The amino acid sequences of the two principal soluble cytochromes c have been determined. One of these is a cytochrome c2, similar in size to mitochondrial cytochrome c. While clearly of the same sequen...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(94)90135-x
更新日期：1994-01-01 00:00:00
abstract：:The present study was conducted on CD4(+) T cells, isolated from wild type (WT) and PPARalpha(null) mice, in order to assess the mechanism of action of docosahexaenoic acid (DHA), an n-3 fatty acid, in the modulation of two transcription factors, i.e., T-bet and GATA-3, implicated in T-cell differentiation towards, re...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2009.09.012
更新日期：2009-11-01 00:00:00
abstract：:This paper deals with the discovery of plasmalogen deficiency in the cerebro-hepato-renal (Zellweger) syndrome and discusses how this has led to the development of postnatal and prenatal diagnostic procedures for this and a number of related peroxisomal disorders in man that show a general impairment in the biosynthes...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(93)90076-5
更新日期：1993-01-01 00:00:00
abstract：:We have analyzed the interference of antitumoral drugs acting through the inhibition of DNA topoisomerase II on the human HeLa cell metabolism. Different compounds characterized by a diverse mechanism of action have been used, namely m-amsacrine, an intercalative drug, etoposide, which does not intercalate DNA, and su...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(95)90009-8
更新日期：1995-01-01 00:00:00
abstract：:Analysis of the nucleotide sequence of 1,400 transfer RNAs has revealed the imprint of a prototypic genetic code in position 3-4-5 of the acceptor stem. It appears only in the transfer RNAs for the primordial amino acids ie those found by chemical condensation of a nitrogen-methane-water-ammonia mixture. The model for...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(90)90033-d
更新日期：1990-05-01 00:00:00
abstract：:A human replication initiation protein, Cdt1, is a central player in the cell cycle regulation of DNA replication, and geminin down-regulates Cdt1 function by direct binding. It has been demonstrated that Cdt1 hyperfunction resulting from Cdt1-geminin imbalance, for example, by geminin silencing with small interfering...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2008.02.018
更新日期：2008-06-01 00:00:00
abstract：:The elucidation of the multiple signaling cascades coupled to the TSH receptor has offered new approaches in the understanding of the pathogenesis of Graves' disease. Here we review findings showing that immunoglobulins from Graves' patients are heterogeneous, bind to different epitopes and, similarly to TSH, activate...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(99)80090-x
更新日期：1999-05-01 00:00:00
abstract：:Concern about the malicious applications of botulinum neurotoxin has highlighted the need for a new generation of safe and highly potent antitoxins. In this study, we developed and evaluated the preclinical pharmacology and safety of a new F(ab')₂ antitoxin against botulinum neurotoxin serotype A (BoNT/A). As an alter...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.06.010
更新日期：2010-10-01 00:00:00
abstract：:Genome-editing technologies, in particular, CRISPR systems, are widely used for targeted regulation of gene expression and obtaining modified human and animal cell lines, plants, fungi, and animals with preassigned features. Despite being well described and easy to perform, the most common methods for construction and...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2019.09.003
更新日期：2019-12-01 00:00:00
abstract：:MicroRNAs (miRNAs) play profound roles in plant growth and development by regulating gene expression. Tetraploid plants often have better physical characteristics and stress tolerance than their diploid progenitors, but the role of miRNAs in this superiority is unclear. Paulownia tomentosa, (Paulowniaceae) is attracti...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2014.02.008
更新日期：2014-07-01 00:00:00
abstract：:Dimethyl selenide (DMSe) and dimethyl diselenide (DMDSe) emissions by soil samples spiked with selenite or (methyl)selenocysteine, with or without a supplement of nutrient broth and glucose were measured. DMSe was the main form of volatile Se produced, and was observed for both Se-substrates. DMDSe was only emitted fr...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2006.09.005
更新日期：2006-11-01 00:00:00
abstract：:This paper reviews recent data on the adenylate cyclase system of the yeast Saccharomyces cerevisiae. Since the discovery of yeast adenylate cyclase mutants and the possibility of molecular biological analysis, adenylate cyclase and the subsequent steps in the cAMP cascade have become subject of intense investigation....
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(85)80228-5
更新日期：1985-01-01 00:00:00
abstract：:The major pterin in Tetrahymena pyriformis, strain W, earlier suggested to be L-threo-biopterin and named ciliapterin [1] is now identified as D-threo-neopterin (D-monapterin). This is the first example of a natural D-monapterin. This compound was characterized by its chromatographic behavior, its fluorescence propert...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(91)90089-j
更新日期：1991-10-01 00:00:00
abstract：:Flavodoxins are one domain alpha/beta electron transfer proteins that participate in photosynthetic reactions. All flavodoxins carry a molecule of flavin mononucleotide (FMN), non-covalently bound, that confers redox properties to the protein. There are two structurally distinct flavodoxins, short ones and long flavod...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(00)88876-8
更新日期：1998-10-01 00:00:00
abstract：:Extracellular Hsp70 (eHsp70) can activate immune cells via Toll-like receptors (TLR) 2 and 4, and induce cytokine synthesis. The aim of this study was to explore inflammation-associated effects of eHsp70 alone and in combination with cigarette smoke extract (CSE) in primary bronchial epithelial cells. We assessed IL-6...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2018.09.010
更新日期：2019-01-01 00:00:00
abstract：:During the suckling period, the rats are fed continuously with milk, which is a high-fat low-carbohydrate diet (HF). At weaning, milk is progressively replaced by the rat's laboratory chow which is a high-carbohydrate low-fat diet (HCHO), and this is accompanied by large hormonal modifications: an increase in plasma i...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(91)90077-e
更新日期：1991-01-01 00:00:00
abstract：:In most cancer cells, the ATP necessary for survival and proliferation is derived from glycolysis rather than from oxidative phosphorylations (OXPHOS) even when oxygen supply would be adequate to sustain them. This phenomenon, named "aerobic glycolysis" by Warburg many years ago, can now be explained by a mechanism up...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2007.03.010
更新日期：2007-09-01 00:00:00
abstract：:NikR proteins are bacterial metallo-regulatory transcription factors that control the expression of the nickel uptake system and/or nickel containing enzymes such as urease, and are involved in the acid stress response. Here, a comparative study is reported on NikR from Helicobacter pylori (HpNikR) and Escherichia col...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2006.07.016
更新日期：2006-11-01 00:00:00
abstract：:The molecular basis for the essential role of cholesterol in mammalian (and other cholesterol-requiring) cells has long been the object of intense interest. Cholesterol has been found to modulate the function of membrane proteins critical to cellular function. Current literature supports two mechanisms for this modula...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(91)90093-g
更新日期：1991-10-01 00:00:00
abstract：:The product of the sex-determining gene SRY is a member of the HMG box containing protein superfamily. The HMG box is a DNA-binding domain of about 80 amino acids shared by many proteins with diverse functions. It seems that the functions of the full length protein are restricted to the HMG box but their molecular bas...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(94)90033-7
更新日期：1994-01-01 00:00:00
