Abstract：
:Flavodoxins are one domain alpha/beta electron transfer proteins that participate in photosynthetic reactions. All flavodoxins carry a molecule of flavin mononucleotide (FMN), non-covalently bound, that confers redox properties to the protein. There are two structurally distinct flavodoxins, short ones and long flavodoxins; the latter contain an extra loop with unknown function. We have undertaken the study of the stability and folding of the apoflavodoxin from Anabaena (a long flavodoxin) and the analysis of the interaction between the apoflavodoxin and FMN. Our studies indicate that apoflavodoxin folds in a few seconds to a form that is competent in FMN binding. The stability of this apoflavodoxin is low and its urea denaturation can be described by a two-state mechanism. The role of the different parts of the apoflavodoxin in the stability and structure of the whole protein is being investigated using mutagenesis and specific cleavage to generate apoflavodoxin fragments. The X-ray structure of apoflavodoxin is very similar to that of its complex with FMN, the main difference being the conformation of the two aromatic residues that sandwich FMN in the complex. In apoflavodoxin these groups interact with each other so closing the FMN binding site. Despite this fact, apoflavodoxin binds FMN tightly and rapidly, and the resulting holoflavodoxin displays a high conformational stability. We have found that one role of the aromatic residues that interact with FMN is to help to retain bound the reduced form of the cofactor whose complex with apoflavodoxin is otherwise too weak.
journal_name
Biochimiejournal_title
Biochimieauthors
Maldonado S,Lostao A,Irún MP,Férnandez-Recio J,Gustavo Genzor C,Begoña González E,Rubio JA,Luquita A,Daoudi F,Sancho Jdoi
10.1016/s0300-9084(00)88876-8subject
Has Abstractpub_date
1998-10-01 00:00:00pages
813-20issue
10eissn
0300-9084issn
1638-6183pii
S0300908400888768journal_volume
80pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:The effect of adenosine was tested on the energetic metabolism of fed rat liver cells after isolation. The cells were incubated in a buffered saline medium with glucose (5 mM) and adenosine (1 mM) for 30 minutes at 37 degrees C. This increased the concentration of the adenylic nucleotides ATP (+57 per cent, ADP (+39 p...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(80)80286-0
更新日期：1979-01-01 00:00:00
abstract：:The Nicotiana tabacum Nt-4/1 protein is a plant-specific protein of unknown function. Analysis of bacterially expressed Nt-4/1 protein in vitro revealed that the protein secondary structure is mostly alpha-helical and suggested that it could consist of three structural domains. Earlier studies of At-4/1, the Arabidops...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.02.015
更新日期：2013-07-01 00:00:00
abstract：:There are five subtypes of muscarinic acetylcholine receptors (M(1) to M(5)) which control a large number of physiological processes, such as the function of heart and smooth muscles, glandular secretion, release of neurotransmitters, gene expression and cognitive functions as learning and memory. A selective ligand i...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(00)01176-7
更新日期：2000-09-01 00:00:00
abstract：:Fluorometry using a substrate DNA labeled with a single fluorophore (6-carboxyfluorescein) at the 3'-end of the processed strand was shown to be a useful tool for monitoring DNA-binding and 3'-processing activities of HIV-1 and PFV integrases (INs). The DNA binding to either of the INs resulted in a fluorescence signa...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2012.06.009
更新日期：2012-11-01 00:00:00
abstract：:The ability to measure the thermodynamic stability of proteins with precision is important for both academic and applied research. Such measurements rely on mathematical models of the protein denaturation profile, i.e. the relation between a global protein signal, corresponding to the folding states in equilibrium, an...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2015.11.013
更新日期：2016-02-01 00:00:00
abstract：:Phospholipid analysis of the membranes associated with fast sedimenting folded chromosomes prepared by lysis of E. coli CR 34 shows that both inner and outer membranes are parts of the complex, in proportions not very different from that found in the whole bacteria. During the preparation of the folded chromosomes, th...
journal_title：Biochimie
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
abstract：:Bacterial superantigens (SAg) are the most potent activators of human T lymphocytes and recombinant immunotoxin using bacterial SAg shows promising clinical values. To engineer superantigen for immunotherapy of hepatocellular carcinoma, we genetically fused the superantigen staphylococcus enterotoxin A (SEA(D(227)A)) ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2005.04.005
更新日期：2005-08-01 00:00:00
abstract：:1) Isonitrile derivatives of synthetic polyamides, polyesters and polyalcohols, of polysaccharides such as cellulose, cross-linked dextran and agarose, linear dextran and of cross-linked and linear polyacrylamide were prepared. 2) Proteins and low-molecular-weight ligands were bound covalently to these materials by fo...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(80)80172-6
更新日期：1980-01-01 00:00:00
abstract：:Epigenetic alterations are involved in every step of carcinogenesis. The development of chromatin-modifying agents (CMAs) has provided the ability to fight cancer by reversing these alterations. Currently, four CMAs have been approved for cancer treatment; two DNA demethylating agents and two deacetylase inhibitors. A...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2012.05.012
更新日期：2012-11-01 00:00:00
abstract：:The targets of in vivo studies of the ribosomal assembly process are mainly the events of rRNA processing, whereas in vitro studies (total reconstitution) focus on principles of the assembly process such as assembly-initiation proteins, rate-limiting steps and a detailed sequence of assembly reactions (assembly map). ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(91)90054-5
更新日期：1991-06-01 00:00:00
abstract：:In 1956, when we started our collaboration, both Alick Isaacs and myself had done previous work on interference between inactive and active influenza viruses. We were aware of the state interference research had reached and of the two alternative explanations that had been envisaged. ...
journal_title：Biochimie
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.biochi.2007.04.007
更新日期：2007-06-01 00:00:00
abstract：:Colorectal cancer (CRC) is the third most diagnosed cancer in the western world, affecting 1 out of approximately 22 people in their lifetime. Several epidemiological studies suggest a positive association between high plasma cholesterol levels and colorectal cancer. However, the molecular mechanisms by which choleste...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2018.07.006
更新日期：2018-10-01 00:00:00
abstract：:Vascular endothelial growth factor (VEGF) and αvβ3 integrin are key molecules that actively participate in tumor angiogenesis and metastasis. Some integrin-blocking molecules are currently under clinical trials for cancer and metastasis treatment. However, the mechanism of action of such inhibitors is not completely u...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2012.04.020
更新日期：2012-08-01 00:00:00
abstract：:The rate of hydrolysis of long chain triglycerides by pure bovine pancreatic lipase has been determined in the presence of variable amounts of bile salts and colipase. Cofactor-free lipase is strongly inhibited by sodium taurodesoxycholate and by mixed bovine bile salts at concentrations higher than the critical micel...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(76)80572-x
更新日期：1975-01-01 00:00:00
abstract：:Mammalian aminoacylase-1 (Acy1) participates in the breakdown of N-acetylated amino acids during intracellular protein catabolism. Acy1 is most abundantly expressed in the kidney tubular epithelium. Lately, Acy1 deficiency was identified in children with increased urinary excretion of several N-acetylamino acids. Here...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2007.12.006
更新日期：2008-05-01 00:00:00
abstract：:Isolation and properties of B. subtilis ts 19 mutant, isolated as thermosensitive for sporulation, are described. At the non permissive temperature (42degreesC), the mutant cells are blocked at stage zero of sporulation and do not excrete extracellular enzymes such as serylprotease and esterase. At the permissive temp...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(76)80361-6
更新日期：1976-01-01 00:00:00
abstract：:We report Raman spectra of various cholinesterases: lytic tetrameric forms (G4) obtained by tryptic digestion of asymmetric acetylcholinesterase (AChE) from Torpedo californica and Electrophorus electricus, a PI-PLC-treated dimeric form (G2) of AChE from T marmorata, and the soluble tetrameric form (G4) of butyrylchol...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(91)90167-y
更新日期：1991-11-01 00:00:00
abstract：:A previous study has shown the effect of individual mineral surfaces on the activity of sweet almond beta-D-glucosidase. We now consider more complex situations likely to occur in soil, such as adsorption onto mixtures of different mineral surfaces, and the effect on enzyme activity of mineral surfaces with organic co...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(87)90197-0
更新日期：1987-06-01 00:00:00
abstract：:Gallium, a group IIIa metal salt, has been demonstrated to be an effective immunosuppressive agent. Gallium has also been shown to inhibit the production of inflammatory cytokines, such as IL-1beta, produced by macrophage-like cells in vitro. To further characterize the effects of gallium on the inflammatory process, ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(00)00384-9
更新日期：2000-02-01 00:00:00
abstract：:Disappearance of 7-ethylguanine and O6-ethylguanine lesions from nuclear DNA is observed on incubation of isolated rat liver nuclei previously treated with ethylnitrosourea. Free 7-ethylguanine but no free O6-ethylguanine is released in the medium. Incubation of methylated or ethylated DNA with chromatin proteins lead...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(82)80132-6
更新日期：1982-08-01 00:00:00
abstract：:Contradictory results for concentrations of vitamin B12, holotranscobalamin (holoTC), and methylmalonic acid (MMA) have been reported. We tested the hypothesis that the extracellular vitamin B12 markers are not reflecting the intracellular vitamin B12-dependent biochemical reactions in individuals with type 2 diabetes...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2012.10.028
更新日期：2013-05-01 00:00:00
abstract：:NADH cytochrome b5 oxidoreductase (Ncb5or) protects β-cells against oxidative stress and lipotoxicity. The predominant phenotype of lean Ncb5or-null mouse is insulin-dependent diabetes due to β-cell death. This suggests the putative role of NCB5OR polymorphism in human diabetes. Therefore, we aimed to investigate the ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.03.004
更新日期：2013-07-01 00:00:00
abstract：:Analysis of the nucleotide sequence of 1,400 transfer RNAs has revealed the imprint of a prototypic genetic code in position 3-4-5 of the acceptor stem. It appears only in the transfer RNAs for the primordial amino acids ie those found by chemical condensation of a nitrogen-methane-water-ammonia mixture. The model for...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(90)90033-d
更新日期：1990-05-01 00:00:00
abstract：:Adenosine to inosine (A-to-I) modification by the ADAR (adenosine deaminase that acts on RNA) enzymes perform the most common type of RNA editing in metazoans. ADARs use double stranded RNA as substrates but allow interruptions of bulges and loops in the structure. It is well known that these enzymes can use messenger...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2007.06.002
更新日期：2007-10-01 00:00:00
abstract：:A novel approach to genetic sequence analysis is presented. This approach, based on compression of algorithms, has been launched simultaneously by Grumbach and Tahi, Milosavljevic and Rivals. To reduce the description of an object, a compression algorithm replaces some regularities in the description by special codes....
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(96)84763-8
更新日期：1996-01-01 00:00:00
abstract：:Endothelial nitric oxide synthase (eNOS) plays a crucial role in the regulation of a variety of cardiovascular functions. Many studies have shown that dietary n-3 polyunsaturated fatty acids (PUFAs) have beneficial effects on coronary atherosclerosis. However, the mechanisms of n-3 PUFAs regulation in eNOS activation ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2006.10.009
更新日期：2007-01-01 00:00:00
abstract：:As the medaka is a popular fish model in genetics, developmental biology and toxicology, the development of an efficient transgenic medaka technique is important for a variety of biological experiments. Here we demonstrated that the maize transposon system, Ac/Ds, greatly improved the transgenesis of microinjected DNA...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.07.006
更新日期：2011-10-01 00:00:00
abstract：:Treatment of wild type Escherichia coli with cis -Pt(NH3)2Cl2 increased the survival and frequency of clear plaques formation of lambda phage damaged by UV radiation. The reactivation process was present in an uvrA mutant and abolished in a lexA host. Trans-Pt(NH3)2Cl2 and [Pt(dien) Cl]Cl (dien = 2HN-CH2-CH2NH-CH2-CH2...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(82)80099-0
更新日期：1982-08-01 00:00:00
abstract：:The issue of whether histone H1 possesses specificity of binding to certain nucleotide sequences in DNA is of fundamental importance to the suggested role of the linker histone in the regulation of gene transcription. The purpose of the present study was to reinvestigate the specificity of binding of histone H1 to the...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(93)90116-a
更新日期：1993-01-01 00:00:00
abstract：:The present study has employed a combination of spectroscopic, calorimetric and computational methods to explore the binding of the three side-chained triazatruxene derivative, termed azatrux, to a human telomeric G-quadruplex sequence, under conditions of molecular crowding. The binding of azatrux to the tetramolecul...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.05.017
更新日期：2011-08-01 00:00:00
