Abstract：
:The present study has employed a combination of spectroscopic, calorimetric and computational methods to explore the binding of the three side-chained triazatruxene derivative, termed azatrux, to a human telomeric G-quadruplex sequence, under conditions of molecular crowding. The binding of azatrux to the tetramolecular parallel [d(TGGGGT)](4) quadruplex in the presence and absence of crowding conditions, was also characterized. The data indicate that azatrux binds in an end-stacking mode to the parallel G-quadruplex scaffold and highlights the key structural elements involved in the binding. The selectivity of azatrux for the human telomeric G-quadruplex relative to another biologically relevant G-quadruplex (c-Kit87up) and to duplex DNA was also investigated under molecular crowding conditions, showing that azatrux has good selectivity for the human telomeric G-quadruplex over the other investigated DNA structures.
journal_name
Biochimiejournal_title
Biochimieauthors
Petraccone L,Fotticchia I,Cummaro A,Pagano B,Ginnari-Satriani L,Haider S,Randazzo A,Novellino E,Neidle S,Giancola Cdoi
10.1016/j.biochi.2011.05.017subject
Has Abstractpub_date
2011-08-01 00:00:00pages
1318-27issue
8eissn
0300-9084issn
1638-6183pii
S0300-9084(11)00174-Xjournal_volume
93pub_type
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