Abstract：
:Although up to 25% of glucose released into circulation in the postabsorptive state comes from renal gluconeogenesis, the regulatory mechanisms of this process are still poorly recognized, comparing to hepatic ones. The aim of the present study was to examine if hypoxia-inducible factor-1 (HIF-1) might be involved in the regulation of glucose de novo synthesis in kidneys. It was found that HK-2 cells (immortalized human kidney proximal tubules, capable of gluconeogenesis/glycogen synthesis) cultured with gluconeogenic substrates either in hypoxia (1% O2) or in the presence of DMOG (an inhibitor of HIF-1α degradation) exhibited increased glycogen content. This phenomenon was not correlated with augmented glucose intake and the effects were reversed by echinomycin (an inhibitor of HIF-1 binding to HRE sequence). As concluded from the measurement of the intracellular content of gluconeogenic intermediates followed by Western blot analysis, under conditions of hypoxia/increased HIF-1 level the activity of phosphoenolpyruvate carboxykinase (PEPCK) was elevated, as a result of increased expression of the cytosolic isoform of PEPCK (PEPCK-C). Chromatin immunoprecipitation (ChIP) analysis proved HIF-1 ability to bind to the promoter region of PEPCK-C gene. The final conclusion that hypoxia/HIF-1 accelerates the rate of renal glucogenesis via the mechanism engaging activation of PEPCK-C expression might be useful in terms of e.g. diabetes treatment, as it is commonly accepted that under diabetic conditions kidneys and liver seem to be equally important sources of glucose synthesized de novo.
journal_name
Biochimiejournal_title
Biochimieauthors
Owczarek A,Gieczewska K,Jarzyna R,Jagielski AK,Kiersztan A,Gruza A,Winiarska Kdoi
10.1016/j.biochi.2020.02.002subject
Has Abstractpub_date
2020-01-01 00:00:00pages
31-37eissn
0300-9084issn
1638-6183pii
S0300-9084(20)30020-1journal_volume
171-172pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:Epidemiological studies have demonstrated that the diabetes mellitus is a serious health burden for both governments and healthcare providers. The present study was hypothesized to evaluate the antihyperglycemic potential of fraxetin by determining the activities of key enzymes of carbohydrate metabolism in streptozot...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.06.013
更新日期：2013-10-01 00:00:00
abstract：:Four full-length cDNAs encoding the precursors of four K(+)-toxin-like peptides (named BmKK(1), BmKK(2), BmKK(3) and BmmKK(4), respectively) were first isolated from a venom gland cDNA library of the Chinese scorpion Buthus martensii Karsch. The deduced precursors of BmKK(1), BmKK(2) and BmKK(3) are all made of 54 ami...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(01)01326-8
更新日期：2001-09-01 00:00:00
abstract：:Sticholysin II (StII) is a pore-forming toxin of biomedical interest that belongs to the actinoporin protein family. Sticholysins are currently under examination as an active immunomodulating component of a vaccinal platform against tumoral cells and as a key element of a nucleic acids delivery system to cell cytosol....
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2018.02.006
更新日期：2018-05-01 00:00:00
abstract：:Several inositol isomers and in particular myo-inositol (MI) and D-chiro-inositol (DCI), were shown to possess insulin-mimetic properties and to be efficient in lowering post-prandial blood glucose. In addition, abnormalities in inositol metabolism are associated with insulin resistance and with long term microvascula...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2013.05.011
更新日期：2013-10-01 00:00:00
abstract：:The present study was conducted on CD4(+) T cells, isolated from wild type (WT) and PPARalpha(null) mice, in order to assess the mechanism of action of docosahexaenoic acid (DHA), an n-3 fatty acid, in the modulation of two transcription factors, i.e., T-bet and GATA-3, implicated in T-cell differentiation towards, re...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2009.09.012
更新日期：2009-11-01 00:00:00
abstract：:From investigation of 2000 soil isolates, we identified two serine-type amidohydrolases that can hydrolyze d-aminoacyl derivatives from the culture supernatant of Streptomyces species 82F2 and 83D12. The enzymes, redesignated as 82F2-DAP and 83D12-DAP, were purified for homogeneity and characterized. Each enzyme had m...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.04.020
更新日期：2011-09-01 00:00:00
abstract：:Investigations have been carried out on the influence of the phospholipid composition and the physicochemical properties of rat liver plasma membranes on the endogenous activity of membrane-bound phospholipase A2. The membrane phospholipid composition was modified by the incorporation of different phospholipids in the...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(87)90153-2
更新日期：1987-11-01 00:00:00
abstract：:Human mitochondrial aminoacyl-tRNA synthetases (mt-aaRSs), the enzymes which esterify tRNAs with the cognate specific amino acid, form mainly a different set of proteins than those involved in the cytosolic translation machinery. Many of the mt-aaRSs are of bacterial-type in regard of sequence and modular structural o...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2009.02.005
更新日期：2009-05-01 00:00:00
abstract：:The interactions of DNA with histone H4 and with its fragments N-H4 (1-84) and C-H4 (85-102) have been studied by using electrooptical techniques, viscosity and electron microscopy. Electron microscopy reveals that histone H4 induces a large folding of DNA molecules : this is in agreement with electrooptical measureme...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(80)80398-1
更新日期：1980-01-01 00:00:00
abstract：:In this study, peptidomics and genomics analyses were used to study antimicrobial peptides from the skin of Hylarana spinulosa. Twenty-nine different antimicrobial peptide precursors were characterized from the skin of H. spinulosa, which produce 23 mature antimicrobial peptides belonging to 12 different families. To ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.09.002
更新日期：2013-12-01 00:00:00
abstract：:Long-term exposure to high doses of caffeine affects several aspects of nitrogen metabolism, such as purine, pyrimidine and urea synthesis. However, little is known about the mechanisms of these changes and if they occur at shorter term. We have studied in isolated hepatocytes: 1) the in vitro effect of high doses of ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(88)90014-4
更新日期：1988-10-01 00:00:00
abstract：:Nitrogen fixation in A. vinelandii and R. leguminosarum bacteroides shows identical characteristics with respect to the dependence on membrane energization, the sensitivity to uncouplers, the ATP/ADP-ratio, and the dependences on flavodoxinhydroquinone as electrondonor. Although we have been successful in preparing in...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(78)80820-7
更新日期：1978-01-01 00:00:00
abstract：:Immobilization of enzymes by crystallization and subsequent cross-linking provide structures characterized by a regular three-dimensional molecular arrangement and a high packing density. Compared to randomly immobilized enzymes, such structures permit more detailed analysis of the variations in kinetic properties ari...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(80)80101-5
更新日期：1980-01-01 00:00:00
abstract：:Total poly (A+) RNA has been extracted from rat pheochromocytoma and translated in vitro by means of a reticulocyte lysate system. We show that two antisera, prepared against pig kidney DOPA decarboxylase (DDC) or rat pheochromocytoma DDC, immunoprecipitate an in vitro synthetized 50 kDa polypeptide identified as DDC ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(90)90175-g
更新日期：1990-01-01 00:00:00
abstract：:In most cases, anti-protein aptamers are selected by systematic evolution of ligands by exponential enrichment (SELEX) using purified recombinant protein targets. Cell surface proteins, however, are not easy targets for SELEX due to the difficulties associated with their purification. Here, we developed a novel SELEX ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2006.02.004
更新日期：2006-07-01 00:00:00
abstract：:Dimethyl selenide (DMSe) and dimethyl diselenide (DMDSe) emissions by soil samples spiked with selenite or (methyl)selenocysteine, with or without a supplement of nutrient broth and glucose were measured. DMSe was the main form of volatile Se produced, and was observed for both Se-substrates. DMDSe was only emitted fr...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2006.09.005
更新日期：2006-11-01 00:00:00
abstract：:Rhizopus oryzae lipase (ROL) was found to be a true lipase. This enzyme presents the interfacial activation phenomenon. The N-terminal amino acid sequence of ROL was compared to those of rhizopus lipases. Purified ROL possesses the same N-terminal sequence as the mature Rhizopus niveus lipase (RNL). This sequence was ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(01)01283-4
更新日期：2001-06-01 00:00:00
abstract：:Ten new defensins have been isolated from seeds of Triticum kiharae and related species of the Triticum and Aegilops genera by a combination of chromatographic procedures including affinity-, size-exclusion, and reversed-phase high-performance liquid chromatography. Nine were completely sequenced and shown to represen...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2006.09.009
更新日期：2007-05-01 00:00:00
abstract：:The analysis of RNA from L5178 Y cells, by electrophoreis in 1.7% polyacrylamide gel, after short time labelling with [methyl-3H] methionine, shows that the three molecular species 47S, 46S and 45S RNA from the ribosomal RNA "45S fraction" are methylated. Comparative studies of the kinetics obtained after labelling fo...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(76)80544-5
更新日期：1975-11-01 00:00:00
abstract：:Bacterial superantigens (SAg) are the most potent activators of human T lymphocytes and recombinant immunotoxin using bacterial SAg shows promising clinical values. To engineer superantigen for immunotherapy of hepatocellular carcinoma, we genetically fused the superantigen staphylococcus enterotoxin A (SEA(D(227)A)) ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2005.04.005
更新日期：2005-08-01 00:00:00
abstract：:The translation factor EF-P is required for efficient prokaryotic peptide bond synthesis on 70S ribosomes from fMet-tRNAfMet. This protein has been purified from Escherichia coli cells and the gene, efp, encoding it has been cloned and sequenced. We have isolated recombinant clones which overexpress a protein that co-...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(97)87619-5
更新日期：1997-01-01 00:00:00
abstract：:Knowing the structure of a protein is essential to characterize its function and mechanism at the molecular level. Despite major advances in solving structures experimentally, most membrane protein native conformations remain unknown. This lack of available structures, along with the physical constraints imposed by th...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2015.05.018
更新日期：2015-08-01 00:00:00
abstract：:Apoptosis is the physiological process by which unwanted cells in an organism are killed. Bcl-2, a membrane-bound cytoplasmic protein, and its close relative Bcl-xL, are both effective inhibitors of apoptosis induced by a wide variety of stimuli in many different cell types. In a previous study, we reported that suppr...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(97)82011-1
更新日期：1997-10-01 00:00:00
abstract：:The mitochondria have arisen as a consequence of endosymbiosis of an ancestral α-proteobacterium with a methane-producing archae. The main function of the canonical aerobic mitochondria include ATP generation via oxidative phosphorylation, heme and phospholipid synthesis, calcium homeostasis, programmed cell death, an...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2013.11.018
更新日期：2014-05-01 00:00:00
abstract：:The mechanism responsible for creating the division site in the right place at the right time in bacteria is unknown. It has been attributed to the formation of proteolipid domains in the cytoplasmic membrane surrounding the nucleoids. We interpret the growing evidence for this hypothesis by invoking hyperstructures, ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(00)01203-7
更新日期：2001-01-01 00:00:00
abstract：:Sialate 9(4)-O-acetylesterases (EC 3.1.1.53) have been isolated from equine liver, bovine brain and influenza C virus. In this latter case, the esterase represents the receptor-destroying enzyme of the virus. The kinetic properties of these enzymes were determined with Neu5,9Ac2 and in part with 4-methylumbelliferyl a...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(88)90288-x
更新日期：1988-11-01 00:00:00
abstract：:Sm and Sm-like (Lsm) proteins are considered as an evolutionary conserved family involved in RNA metabolism in organisms from bacteria and archaea to human. Currently, the function of Sm-like archaeal proteins (SmAP) is not well understood. Here, we report the crystal structures of SmAP proteins from Sulfolobus acidoc...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.05.001
更新日期：2020-08-01 00:00:00
abstract：:A previous study has shown the effect of individual mineral surfaces on the activity of sweet almond beta-D-glucosidase. We now consider more complex situations likely to occur in soil, such as adsorption onto mixtures of different mineral surfaces, and the effect on enzyme activity of mineral surfaces with organic co...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(87)90197-0
更新日期：1987-06-01 00:00:00
abstract：:The molecular basis for the essential role of cholesterol in mammalian (and other cholesterol-requiring) cells has long been the object of intense interest. Cholesterol has been found to modulate the function of membrane proteins critical to cellular function. Current literature supports two mechanisms for this modula...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(91)90093-g
更新日期：1991-10-01 00:00:00
abstract：:The poisonous bite by Hippasa partita, a funnel web spider from the Indian subcontinent has been demonstrated to give rise to severe dermo- and myonecrosis. In this work a hemorrhagic metalloprotease, Partitagin was purified from H. partita venom by successive chromatography on Sephadex G-100, DEAE Sephadex A-50 and B...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2007.04.005
更新日期：2007-11-01 00:00:00
