Hypoxia increases the rate of renal gluconeogenesis via hypoxia-inducible factor-1-dependent activation of phosphoenolpyruvate carboxykinase expression.

Abstract:

:Although up to 25% of glucose released into circulation in the postabsorptive state comes from renal gluconeogenesis, the regulatory mechanisms of this process are still poorly recognized, comparing to hepatic ones. The aim of the present study was to examine if hypoxia-inducible factor-1 (HIF-1) might be involved in the regulation of glucose de novo synthesis in kidneys. It was found that HK-2 cells (immortalized human kidney proximal tubules, capable of gluconeogenesis/glycogen synthesis) cultured with gluconeogenic substrates either in hypoxia (1% O2) or in the presence of DMOG (an inhibitor of HIF-1α degradation) exhibited increased glycogen content. This phenomenon was not correlated with augmented glucose intake and the effects were reversed by echinomycin (an inhibitor of HIF-1 binding to HRE sequence). As concluded from the measurement of the intracellular content of gluconeogenic intermediates followed by Western blot analysis, under conditions of hypoxia/increased HIF-1 level the activity of phosphoenolpyruvate carboxykinase (PEPCK) was elevated, as a result of increased expression of the cytosolic isoform of PEPCK (PEPCK-C). Chromatin immunoprecipitation (ChIP) analysis proved HIF-1 ability to bind to the promoter region of PEPCK-C gene. The final conclusion that hypoxia/HIF-1 accelerates the rate of renal glucogenesis via the mechanism engaging activation of PEPCK-C expression might be useful in terms of e.g. diabetes treatment, as it is commonly accepted that under diabetic conditions kidneys and liver seem to be equally important sources of glucose synthesized de novo.

journal_name

Biochimie

journal_title

Biochimie

authors

Owczarek A,Gieczewska K,Jarzyna R,Jagielski AK,Kiersztan A,Gruza A,Winiarska K

doi

10.1016/j.biochi.2020.02.002

subject

Has Abstract

pub_date

2020-01-01 00:00:00

pages

31-37

eissn

0300-9084

issn

1638-6183

pii

S0300-9084(20)30020-1

journal_volume

171-172

pub_type

杂志文章
  • Oxidative phosphorylation in intact chl-r mutants of Escherichia coli K 12.

    abstract::The efficiency of oxidative phosphorylation was estimated in intact resting cells of Escherichia coli K 12, strain PA 601 (chl-s) and its chl-r mutants, all of them grown anaerobically in the presence of nitrate. The oxidation of endogenous NADH in intact chl-s cells was accompanied by the formation of ATP whatever th...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(77)80316-7

    authors: Giordano G,Rivière C,Azoulay E

    更新日期:1977-01-01 00:00:00

  • [Typical tripeptide sequences of collagen: quantitative conformation study].

    abstract::Theoretical and experimental investigations on the conformations of synthetic polytripeptides, with Gly-R2-R3 general repetitive sequence, are of great interest for the comprehension of the factors which contribute to the triple helix stabilization. Using appropriate molecules as models, we have investigated by the PC...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:

    authors: Cabrol D,Broch H,Vasilescu D

    更新日期:1981-11-01 00:00:00

  • Hypothesis: membrane domains and hyperstructures control bacterial division.

    abstract::The mechanism responsible for creating the division site in the right place at the right time in bacteria is unknown. It has been attributed to the formation of proteolipid domains in the cytoplasmic membrane surrounding the nucleoids. We interpret the growing evidence for this hypothesis by invoking hyperstructures, ...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/s0300-9084(00)01203-7

    authors: Norris V,Fishov I

    更新日期:2001-01-01 00:00:00

  • Distinct functional properties of secretory l-asparaginase Rv1538c involved in phagosomal survival of Mycobacterium tuberculosis.

    abstract::The emergence of drug-resistant Mycobacterium tuberculosis (Mtb) stains has escalated the need for developing more efficient drugs and therapeutic strategies against tuberculosis. Here we functionally annotate a secretory mycobacterial asparaginase Rv1538c (MtA) and describe its biochemical properties. MtA primarily e...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2020.12.023

    authors: Kataria A,Patel AK,Kundu B

    更新日期:2021-01-05 00:00:00

  • Effect of beta-agonists on expression of calpain and calpastatin activity in skeletal muscle.

    abstract::Administration of beta-adrenergic agonists to domestic species can lead to skeletal muscle hypertrophy, probably by reducing the rate of myofibrillar protein breakdown. Myofibrillar breakdown is associated with the calcium-dependent proteinase system (calpains I,II and calpastatin) whose activity also changes during b...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(92)90125-x

    authors: Bardsley RG,Allcock SM,Dawson JM,Dumelow NW,Higgins JA,Lasslett YV,Lockley AK,Parr T,Buttery PJ

    更新日期:1992-03-01 00:00:00

  • Ayadualin, a novel RGD peptide with dual antihemostatic activities from the sand fly Lutzomyia ayacuchensis, a vector of Andean-type cutaneous leishmaniasis.

    abstract::Sequence analysis of the Lutzomyia (Lu.) ayacuchensis salivary gland cDNA library identified a short peptide containing an RGD (Arg-Gly-Asp) sequence flanked by two cysteine residues in the C-terminal end as the most abundant transcript. In the present study, a recombinant protein of the RGD-containing peptide, design...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2015.02.011

    authors: Kato H,Gomez EA,Fujita M,Ishimaru Y,Uezato H,Mimori T,Iwata H,Hashiguchi Y

    更新日期:2015-05-01 00:00:00

  • Epigenetic modifications and mRNA levels of the imprinted gene Grb10 in serially passaged fibroblast cells.

    abstract::The imprinted gene Grb10 influences fetal growth, adult behavior and tumor formation. Investigating this gene often involves consecutive passaging of cells in vitro. We analyzed changes in Grb10 DNA methylation and histone modifications in serially passaged adult mouse tail-tip fibroblast cells with bisulfite genomic ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2012.08.009

    authors: Yao J,Huang Y,Huang R,Shi R,Chen P,Zhu B,Li M,Jiang X,Zheng M,Jiang Y,Yang X

    更新日期:2012-12-01 00:00:00

  • Fate of the methyl group during the conversion of sterigmatocystin into O-methylsterigmatocystin and aflatoxin B1 by cell-free preparations of Aspergillus parasiticus.

    abstract::Cell-free extracts of fungal mycelia of two aflatoxin non-producing isolates of Aspergillus parasiticus (SRRC 163 and SRRC 2043) were utilized for the study of enzyme activities involved in the latter stages of aflatoxin biosynthesis. The post-microsomal fractions (105,000 x g supernatant) of both SRRC 163 and SRRC 20...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(88)90103-4

    authors: Bhatnagar D,Cleveland TE

    更新日期:1988-06-01 00:00:00

  • Characterization of papain-like isoenzymes from latex of Asclepias curassavica by molecular biology validated by proteomic approach.

    abstract::Latices from Asclepias spp are used in wound healing and the treatment of some digestive disorders. These pharmacological actions have been attributed to the presence of cysteine proteases in these milky latices. Asclepias curassavica (Asclepiadaceae), "scarlet milkweed" is a perennial subshrub native to South America...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2009.07.017

    authors: Obregón WD,Liggieri CS,Trejo SA,Avilés FX,Vairo-Cavalli SE,Priolo NS

    更新日期:2009-11-01 00:00:00

  • A novel antimicrobial peptide, scolopendin, from Scolopendra subspinipes mutilans and its microbicidal mechanism.

    abstract::A novel antimicrobial peptide (AMP) was identified from the centipede Scolopendra subspinipes mutilans by RNA sequencing, and the amino acid sequences predicted from the sequenced mRNAs were compared with those of known AMPs. We named this peptide scolopendin, according to its origin, and investigated the molecular me...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2015.08.015

    authors: Lee W,Hwang JS,Lee DG

    更新日期:2015-11-01 00:00:00

  • Inhibitors of specific ceramide synthases.

    abstract::Ceramide synthases (CerSs) are key enzymes in the biosynthesis of ceramides and display a group of at least six different isoenzymes (CerS1-6). Ceramides itself are bioactive molecules. Ceramides with different N-acyl side chains (C(14:0)-Cer - C(26:0)-Cer) possess distinct roles in cell signaling. Therefore, the sele...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2011.09.007

    authors: Schiffmann S,Hartmann D,Fuchs S,Birod K,Ferreiròs N,Schreiber Y,Zivkovic A,Geisslinger G,Grösch S,Stark H

    更新日期:2012-02-01 00:00:00

  • The insulin receptor kinase.

    abstract::The insulin receptor appears as a tetrameric glycoprotein consisting of two Mr 130,000 subunits (alpha), and two Mr 95,000 subunits (beta) in a disulfide-linked complex. Insulin bound to its specific cell surface receptors in its target cells leads to a complex array of molecular events resulting in insulin effects. I...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(85)80109-7

    authors: Van Obberghen E,Ballotti R,Gazzano H,Fehlmann M,Rossi B,Gammeltoft S,Debant A,Le Marchand-Brustel Y,Kowalski A

    更新日期:1985-10-01 00:00:00

  • A-to-I editing challenger or ally to the microRNA process.

    abstract::Adenosine to inosine (A-to-I) modification by the ADAR (adenosine deaminase that acts on RNA) enzymes perform the most common type of RNA editing in metazoans. ADARs use double stranded RNA as substrates but allow interruptions of bulges and loops in the structure. It is well known that these enzymes can use messenger...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/j.biochi.2007.06.002

    authors: Ohman M

    更新日期:2007-10-01 00:00:00

  • [Monkey liver microsomal glucose-6-phosphatase].

    abstract::Kinetic studies indicate that glucose-6-phosphatase is a multifunctional enzyme. a) Phosphohydrolase activities. The mannose-6-phosphatase activity is low (Km = 8 mM, VM = 90 nmoles. min-1mg-1). The enzyme shows a strong affinity for glucose-6-phosphate (Km = 2.5 mM, VM = 220 nmoles.min-1mg-1). beta-glycerophosphate (...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:

    authors: Benedetto JP,Martel MB,Got R

    更新日期:1979-01-01 00:00:00

  • In vitro translation of human pheochromocytoma messenger RNAs: characterization of tyrosine-hydroxylase and dopamine-beta-hydroxylase.

    abstract::mRNAs extracted from human pheochromocytoma were translated in vitro in a lysate of a rabbit reticulocytes. Two enzymes of the biosynthetic pathway of the catecholamines, tyrosine-hydroxylase (TH) and dopamine-beta-hydroxylase (DBH), were characterized as translation products after immunoprecipitation by specific anti...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(85)80198-x

    authors: Benlot C,Antreassian J,Henry JP,Legrand JC,Gros F,Thibault J

    更新日期:1985-06-01 00:00:00

  • Differential contributions of recognition factors of two plant lectins -Amaranthus caudatus lectin and Arachis hypogea agglutinin, reacting with Thomsen-Friedenreich disaccharide (Galbeta1-3GalNAcalpha1-Ser/Thr).

    abstract::Previous reports on the carbohydrate specificities of Amaranthus caudatus lectin (ACL) and peanut agglutinin (PNA, Arachis hypogea) indicated that they share the same specificity for the Thomsen-Friedenreich (T(alpha), Galbeta1-3GalNAcalpha1-Ser/Thr) glycotope, but differ in monosaccharide binding--GalNAc>Gal (inactiv...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2008.08.001

    authors: Wu AM,Wu JH,Yang Z,Singh T,Goldstein IJ,Sharon N

    更新日期:2008-11-01 00:00:00

  • Is carotenoid ornamentation linked to the inner mitochondria membrane potential? A hypothesis for the maintenance of signal honesty.

    abstract::Several mechanistic hypotheses have been proposed for how carotenoid pigmentation of integumentary structures can serve as an honest signal of individual quality. These hypotheses are founded on proposed links between carotenoids, immuno responsiveness, and oxidative stress, but an absence of biochemical information o...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2012.10.021

    authors: Johnson JD,Hill GE

    更新日期:2013-02-01 00:00:00

  • VaH3, one of the principal hemorrhagins in Vipera ammodytes ammodytes venom, is a homodimeric P-IIIc metalloproteinase.

    abstract::Hemorrhage is the most potent manifestation of envenomation by Vipera ammodytes ammodytes (V. a. ammodytes) venom in man. A detailed description of the venom components contributing to this effect is thus medically very important. We have characterized a novel component, termed here VaH3, as a potently hemorrhagic sna...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2013.01.003

    authors: Sajevic T,Leonardi A,Kovačič L,Lang-Balija M,Kurtović T,Pungerčar J,Halassy B,Trampuš-Bakija A,Križaj I

    更新日期:2013-06-01 00:00:00

  • Site-selected introduction of modified purine and pyrimidine ribonucleosides into RNA by automated phosphoramidite chemistry.

    abstract::The study of modified nucleoside contributions to RNA chemistry, structure and function has been thwarted by the lack of a site-selected method of incorporation which is both versatile and adaptable to present synthetic technologies. A reproducible and versatile site-selected incorporation of nine differently modified...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(96)88115-6

    authors: Agris PF,Malkiewicz A,Kraszewski A,Everett K,Nawrot B,Sochacka E,Jankowska J,Guenther R

    更新日期:1995-01-01 00:00:00

  • From green chemistry to nature: The versatile role of low transition temperature mixtures.

    abstract::In 1998, the concept of "green chemistry" was established through twelve principles with the aim of improving the eco-efficiency of chemical processes and to judge, whether or not, a chemical process is sustainable. Currently, numerous processes do not obey to most of these principles (large energy usage, formation of...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/j.biochi.2015.09.019

    authors: Durand E,Lecomte J,Villeneuve P

    更新日期:2016-01-01 00:00:00

  • Techniques for the removal of marker genes from transgenic plants.

    abstract::The presence of marker genes encoding antibiotic or herbicide resistances in genetically modified plants poses a number of problems. Various techniques are under development for the removal of unwanted marker genes, while leaving required transgenes in place. The aim of this brief review is to describe the principal m...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/s0300-9084(02)00021-4

    authors: Scutt CP,Zubko E,Meyer P

    更新日期:2002-11-01 00:00:00

  • [Energy-dependent translocation of amino-phospholipids in the erythrocyte membrane].

    abstract::In this review, we show how the stability of the asymmetric transverse distribution of phospholipids and the physiological role of the asymmetric distribution can be explained. Experiments with paramagnetic or fluorescent lipids enabled us to show that in fresh red blood cells, i.e. containing ATP, and in resealed gho...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/s0300-9084(86)80005-0

    authors: Devaux PF,Zachowski A,Favre E,Fellmann P,Cribier S,Geldwerth D,Hervé P,Seigneuret M

    更新日期:1986-03-01 00:00:00

  • Efficient adenoviral transduction of 3T3-F442A preadipocytes without affecting adipocyte differentiation.

    abstract::The preadipocyte cell lines 3T3-L1 and 3T3-F442A are widely used to study the cellular mechanisms of preadipocyte differentiation and mature adipocyte functions. However, transfection with naked DNA is inefficient in these cell lines. Adenoviral gene transfer is a powerful technique to induce high levels of transgene ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2005.05.010

    authors: Béréziat V,Moritz S,Klonjkowski B,Decaudain A,Auclair M,Eloit M,Capeau J,Vigouroux C

    更新日期:2005-11-01 00:00:00

  • Implication of apoE isoforms in cholesterol metabolism by primary rat hippocampal neurons and astrocytes.

    abstract::Apolipoprotein E (apoE) has been genetically linked to late-onset Alzheimer's disease. From the three common alleles (epsilon2, epsilon3 and epsilon4), epsilon4 has been suggested to promote amyloid beta (Ass) plaque fibrillation, one hallmark of Alzheimer's disease. It has been demonstrated that altered lipid content...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2005.10.007

    authors: Rapp A,Gmeiner B,Hüttinger M

    更新日期:2006-05-01 00:00:00

  • 27-hydroxycholesterol decreases cell proliferation in colon cancer cell lines.

    abstract::Colorectal cancer (CRC) is the third most diagnosed cancer in the western world, affecting 1 out of approximately 22 people in their lifetime. Several epidemiological studies suggest a positive association between high plasma cholesterol levels and colorectal cancer. However, the molecular mechanisms by which choleste...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2018.07.006

    authors: Warns J,Marwarha G,Freking N,Ghribi O

    更新日期:2018-10-01 00:00:00

  • Potential role and therapeutic interests of myo-inositol in metabolic diseases.

    abstract::Several inositol isomers and in particular myo-inositol (MI) and D-chiro-inositol (DCI), were shown to possess insulin-mimetic properties and to be efficient in lowering post-prandial blood glucose. In addition, abnormalities in inositol metabolism are associated with insulin resistance and with long term microvascula...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/j.biochi.2013.05.011

    authors: Croze ML,Soulage CO

    更新日期:2013-10-01 00:00:00

  • Regulation of translation and cell growth by eIF-4E.

    abstract::This review discusses the regulation of a key controlling step in the initiation of protein synthesis, the binding of mRNA to ribosomes. Particular focus is given to the phosphorylation of the cap-binding factor, eIF-4E, and the role of this factor in the regulation of cell growth. ...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/0300-9084(94)90185-6

    authors: Sonenberg N

    更新日期:1994-01-01 00:00:00

  • Distribution and genetic diversity of bacterial thiopurine methyltransferases in soils emitting dimethyl selenide.

    abstract::Dimethyl selenide (DMSe) and dimethyl diselenide (DMDSe) emissions by soil samples spiked with selenite or (methyl)selenocysteine, with or without a supplement of nutrient broth and glucose were measured. DMSe was the main form of volatile Se produced, and was observed for both Se-substrates. DMDSe was only emitted fr...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2006.09.005

    authors: Favre-Bonté S,Ranjard L,Champier L,Cournoyer B,Nazaret S

    更新日期:2006-11-01 00:00:00

  • Biochemical changes induced by strontium ranelate in differentiating adipocytes.

    abstract::Low bone formation in osteoporosis is associated with a shift from osteoblastic to adipogenic differentiation of mesenchymal stem cells (MSC) inducing a concomitant lipotoxic milieu within the bone marrow. Strontium ranelate (SrRN), a treatment for osteoporosis, has both anti-resorptive and anabolic effects on bone. T...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2012.11.008

    authors: Vidal C,Gunaratnam K,Tong J,Duque G

    更新日期:2013-04-01 00:00:00

  • Membrane viscosity is a major modulating factor of the enterocin CRL35 activity.

    abstract::Enterocin CRL35 activity is deeply influenced by the membrane viscosity as could be demonstrated performing determinations of the minimal inhibitory concentrations (MIC) at different temperatures and analyzing the membrane viscosity in these cells as well as in resistant bacteria. In all the cases, bacteriocin activit...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2004.10.013

    authors: Minahk CJ,Saavedra L,Sesma F,Morero R

    更新日期:2005-02-01 00:00:00