Abstract：
:HIV-1 reverse transcriptase uses the host tRNA3(Lys) as a primer for the synthesis of the minus DNA strand. The first event in viral replication is thus the annealing of tRNA to the primer binding site (PBS) in the 5' UTR of the viral RNA. This event requires a major RNA rearrangement which is chaperoned by the viral NC protein. The binding of NC to nucleic acids is essentially non-specific, however, NC is known to bind selectively to hairpins located in the 5' region of the viral RNA. In a previous study, using an NMR approach in which the reaction is slowed down by controlling temperature, we were able to follow details in this RNA unfolding/refolding process and to uncover an intermediate state. We showed that annealing initiates at the junction between the acceptor and the TPsiC stems, and that, at physiological temperature, complete annealing is reached only in the presence of NC, probably when the zinc fingers contact the TPsiC/D loops. In the present work, we have refined our model of the formation of the tRNA3(Lys)/PBS duplex. First, we show that annealing can initiate both from the single-stranded CCA 3'-end bases of the acceptor stem and from the bases in the TPsiC stem. Secondly, by NMR and fluorescence spectroscopy, we have studied the complex between the NC protein and RNA hairpins that mimic the D and T arms of the tRNA3(Lys). Interestingly, the NC protein shows strong and specific binding to the D arm of tRNA3(Lys), which could explain the overall annealing mechanism.
journal_name
Biochimiejournal_title
Biochimieauthors
Barraud P,Gaudin C,Dardel F,Tisné Cdoi
10.1016/j.biochi.2007.01.016subject
Has Abstractpub_date
2007-10-01 00:00:00pages
1204-10issue
10eissn
0300-9084issn
1638-6183pii
S0300-9084(07)00028-4journal_volume
89pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:The incubation of polyphosphates with the ribosomal fraction of Sulfolobus acidocaldarius leads to the covalent attachment of phosphate to threonine residue(s) of a single 40,000 Mr protein. The hydrolysis kinetics of this protein showed that polyphosphate might be the modifying group. The reaction requires 2 mM Mn2+ ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(89)90115-6
更新日期：1989-09-01 00:00:00
abstract：:Kinetic properties of PGM1 and PGM2 phosphoglucomutase "primary" isoenzymes from human erythrocytes were studied. The two enzyme forms share a "ping-pong" kinetic mechanism and show similar Km for substrate (glucose 1-P) and cofactor (glucose 1,6-P2). Micromolar concentrations of fructose 1,6-P2 and glycerate 2,3-P2 i...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(84)90115-9
更新日期：1984-09-01 00:00:00
abstract：:The wheat germ translation initiation factor 2 (WGeIF-2) was isolated in a homogeneous state by an efficient procedure and characterized. Its molecular mass, as determined by a gel-filtration method is approximately 150,000 Da. According to SDS-PAGE WGeIF-2 consists of four subunits with M(r) 37,000 (alpha), 40,000 (b...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(92)90085-s
更新日期：1992-05-01 00:00:00
abstract：:Wheat germ lysate was used as a model system for in vitro translation. We show that an increase of the exchange surface between the reaction mixture and the atmosphere enhanced the amount of incorporated cysteine, indicating that early arrest of protein synthesis previously observed in such a system was due to oxygen ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(96)88123-5
更新日期：1995-01-01 00:00:00
abstract：:Experimental Lyme arthritis provides a mouse model for exploring the development of pathology following infection of C3H mice with Borrelia burgdorferi. Infected mice develop a reliable inflammatory arthritis of the ankle joint with severity that typically peaks around two to three weeks post-infection and then underg...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.06.010
更新日期：2017-10-01 00:00:00
abstract：:Cell-to-cell movement of Poa semilatent virus (genus Hordeivirus) in infected plants is mediated by three viral 'triple gene block' (TGB) proteins. One of those termed TGBp3 is an integral membrane protein essential for intracellular transport of other TGB proteins and viral genomic RNA to plasmodesmata. TGBp3 targeti...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.01.002
更新日期：2011-04-01 00:00:00
abstract：:Porcine pancreatic extracts (PPE), also named pancreatin, are commonly used as a global source of pancreatic enzymes for enzyme replacement therapy in patients with exocrine pancreatic insufficiency. They are considered as a good substitute of human pancreatic enzymes and they have become a material of choice for in v...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2019.07.004
更新日期：2020-02-01 00:00:00
abstract：:Snake venom phospholipases A2 (PLA2) share high sequence identities and a conserved structural scaffold, but show important functional differences. Only a few PLA2s have been purified and characterized from coral snake (Micrurus spp.) venoms, and their role in envenomation remains largely unknown. In this report, we d...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.03.008
更新日期：2017-06-01 00:00:00
abstract：:Human telomeres are intrinsically dynamic structures, with multiple biological processes operating to generate substantial length heterogeneity. Processes that operate specifically at the terminus, that include the end-replication problem coupled with C-strand resection, result in gradual telomere erosion with ongoing...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2007.08.003
更新日期：2008-01-01 00:00:00
abstract：:The published literature contains a number of examples of normally non-cytoplasmic proteins whose transport out of the cytoplasm is not completely abolished by drastic alterations to their routing signals (signal sequences, etc). Furthermore, there are numerous examples of cytoplasmic proteins that can be routed to an...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(90)90133-2
更新日期：1990-02-01 00:00:00
abstract：:The inhibition rates and spectral characteristics of 2 probes specific for the active-site serine residue of proteases were examined for evidence of conformational change of the proteases upon their binding to alpha 2-macroglobulin (alpha 2M). Elastase, chymotrypsin, trypsin, and plasmin were reacted with (7-nitrobenz...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(89)90004-7
更新日期：1989-03-01 00:00:00
abstract：:The hepatitis E virus (HEV) is the most common cause of acute hepatitis worldwide. Although HEV is a small, naked RNA virus, HEV particles become associated with lipids in the blood of infected patients and in the supernatant of culture systems. The egress of these particles from cells implies the exocytosis pathway b...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.05.003
更新日期：2017-10-01 00:00:00
abstract：:Bikunin (Bk) is a Künitz-type serine proteinase inhibitor, which occurs in human plasma, mainly as covalent complexes with one or two of the three peptide heavy chains. The leading member of this glycoprotein family is inter-alpha-inhibitor (I alpha I), which consists of two heavy chains (H1 and H2) linked to Bk. Bk c...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(03)00066-x
更新日期：2003-01-01 00:00:00
abstract：:Split luciferase complementary assay has been used to investigate the effect of WD domain deletion on Apaf-1 oligomerization. Apaf-1 is an adaptor molecule in formation of apoptosome that activates caspase-9, an activation that is a key event in the mitochondrial cell death pathway. Structural studies suggest that nor...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.10.013
更新日期：2021-01-01 00:00:00
abstract：:The combination of cDNA and RNA sequencing techniques has enabled determination of the complete sequence of one of the mRNAs coding for the precursor of the small subunit of ribulose bisphosphate carboxylase of Nicotiana sylvestris. In this 898-nucleotide-long mRNA, 540 nucleotides code for the entire 180-amino-acid-l...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(84)90148-2
更新日期：1984-07-01 00:00:00
abstract：:This review discusses the regulation of a key controlling step in the initiation of protein synthesis, the binding of mRNA to ribosomes. Particular focus is given to the phosphorylation of the cap-binding factor, eIF-4E, and the role of this factor in the regulation of cell growth. ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(94)90185-6
更新日期：1994-01-01 00:00:00
abstract：:Two beta-D-glycosidases have been purified from the digestive juice of Achatina balteata by acetone fractionation, ion exchange chromatography through DEAE-Sephadex A-50, ammonium sulphate fractionation and gel chromatography through Sephadex G-200. The preparations are homogeneous by p/lyacrylamide gel electrophoresi...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(77)80167-3
更新日期：1977-01-01 00:00:00
abstract：:We have previously shown that withdrawal of folic acid led to metabolic reprogramming and a less aggressive phenotype in a mouse cell model of triple-negative breast cancer (TNBC). Herein, we evaluate the effects of folic acid withdrawal on transcriptomic profiles in these cells. Murine cell lines were originally deri...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.04.005
更新日期：2020-06-01 00:00:00
abstract：:Snake venom Kunitz-type serine protease inhibitors (KSPIs) exhibit various biological functions including anticoagulant activity. This study elucidates the occurrence and subunit stoichiometry of a putative complex formed between two KSPIs (Rusvikunin and Rusvikunin-II) purified from the native Rusvikunin complex of P...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2016.08.005
更新日期：2016-09-01 00:00:00
abstract：:Alpha 1, 2-fucosyltransferase (FUT 1/2) is a rate-limiting enzyme that catalyzes the synthesis of Lewis y, a cell membrane-associated carbohydrate antigen. In human ovarian cancer, the upregulated expression of FUT1 and Lewis y is associated with advanced pathological stages and involved in cell proliferation, migrati...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2014.09.015
更新日期：2014-12-01 00:00:00
abstract：:Two forms of baker's yease valyl-tRNA synthetase have been purified to apparent homogeneity by classical methods. It was demonstrated that one of the two forms of the enzyme originates from the other by proteolysis, the respective amounts of each form depending on the physiological state of the yeast. The species main...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(76)80579-2
更新日期：1975-01-01 00:00:00
abstract：:Well-defined quantities of *OH, O2*-,HO2* or RO2*)radicals (reactive oxygen species) can be specifically produced by radiolysis of water or ethanol. Such radical species can initiate one-electron oxidation or one-electron reduction reactions on numerous biological systems. The oxidative hypothesis of atherosclerosis c...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2004.10.007
更新日期：2004-12-01 00:00:00
abstract：:Directed mutagenesis has been used to study the nicotinamide subsite of the glycolytic NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Residue Asn313 is involved together with the carboxyamide moiety of the nicotinamide ring in a complex network of hydrogen bonding interactions which fix the position o...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(90)90119-2
更新日期：1990-08-01 00:00:00
abstract：:Poly(ADP-ribosyl)ation is a posttranslational protein modification significant for genomic stability and cell survival in response to DNA damage. Poly(ADP-ribosyl)ation is catalyzed by poly(ADP-ribose)polymerases (PARPs). Among the 17 members of the PARP family, PARP-1 and PARP-2 are described as enzymes whose catalyt...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.01.007
更新日期：2013-06-01 00:00:00
abstract：:Various phosphonic and sulfonic glutamate analogues as well as phosphonopeptides related to glutathione were studied for their interaction with rat kidney gamma-glutamylcysteine synthetase activity. We found, in all cases, that the presence of a phosphonic group increases the affinity for the enzyme. Among the tripept...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(87)90083-6
更新日期：1987-05-01 00:00:00
abstract：BACKGROUND:The modest efficacy of available therapies for Hepatocellular carcinoma (HCC) indicates the need to develop novel therapeutic approaches. For the proteasome inhibitor Bortezomib (BZB), potentially attractive for HCC treatment, the mechanism of action is largely unknown. The BZB effect on E2Fs and the E2Fs co...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2015.02.015
更新日期：2015-05-01 00:00:00
abstract：:The product of the spoIIM gene of Bacillus subtilis is required for complete septum migration and forespore engulfment during sporulation. To investigate whether expression of spoIIM is required in the forespore compartment of the sporangium, we have constructed a new integrational vector, pKSV7, which contains temper...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(92)90143-3
更新日期：1992-07-01 00:00:00
abstract：:The major pterin in Tetrahymena pyriformis, strain W, earlier suggested to be L-threo-biopterin and named ciliapterin [1] is now identified as D-threo-neopterin (D-monapterin). This is the first example of a natural D-monapterin. This compound was characterized by its chromatographic behavior, its fluorescence propert...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(91)90089-j
更新日期：1991-10-01 00:00:00
abstract：:The yeast Saccharomyces cerevisiae strain LB332 bearing a mutation in the ERG20 gene encoding farnesyl diphosphate synthase (FPPS) synthesizes significantly longer dolichols than the wild type strain FL100 (14-31 and 14-19 isoprene units, respectively). The measurement of the short chain prenyl alcohols excreted into ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(00)01155-x
更新日期：2000-08-01 00:00:00
abstract：:The G protein coupled receptors (GPCR) are an important class of proteins that act as signal transducers through the cytoplasmic membrane. Understanding the structure and activation mechanism of these proteins is crucial for understanding many different aspects of cellular signalling. The olfactory receptors correspon...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(98)80019-9
更新日期：1998-02-01 00:00:00
