当前位置： SCI文献检索 > BIOCHIMIE期刊下所有文献 > Primary structures and partial toxicological characterization of two phospholipases A2 from Micrurus mipartitus and Micrurus dumerilii coral snake venoms.

Primary structures and partial toxicological characterization of two phospholipases A2 from Micrurus mipartitus and Micrurus dumerilii coral snake venoms.

Abstract：

:Snake venom phospholipases A2 (PLA2) share high sequence identities and a conserved structural scaffold, but show important functional differences. Only a few PLA2s have been purified and characterized from coral snake (Micrurus spp.) venoms, and their role in envenomation remains largely unknown. In this report, we describe the isolation, sequencing and partial functional characterization of two Micrurus PLA2s: MmipPLA2 from Micrurus mipartitus and MdumPLA2 from Micrurus dumerilii, two species of clinical importance in Colombia. MmipPLA2 consisted of 119 amino acid residues with a predicted pI of 8.4, whereas MdumPLA2 consisted of 117 residues with a pI of 5.6. Both PLA2s showed the conserved 'group I' cysteine pattern and were enzymatically active, although MdumPLA2 had higher activity. The two enzymes differed notably in their toxicity, with MmipPLA2 being highly lethal to mice and mildly myotoxic, whereas MdumPLA2 was not lethal (up to 3 μg/g body weight) but strongly myotoxic. MdumPLA2 displayed higher anticoagulant activity than MmipPLA2in vitro and caused more sustained edema in the mouse footpad assay. Neither of these enzymes was cytolytic to cultured skeletal muscle C2C12 myotubes. Based on their structural differences, the two enzymes were placed in separate lineages in a partial phylogeny of Micrurus venom PLA2s and this classification agreed with their divergent biological activities. Overall, these findings highlight the structural and functional diversity of Micrurus venom PLA2s.

journal_name

Biochimie

journal_title

Biochimie

authors

Rey-Suárez P,Núñez V,Saldarriaga-Córdoba M,Lomonte B

doi

10.1016/j.biochi.2017.03.008

subject

Has Abstract

pub_date

2017-06-01 00:00:00

pages

88-98

eissn

0300-9084

issn

1638-6183

pii

S0300-9084(17)30061-5

journal_volume

137

pub_type

杂志文章

相关文献

BIOCHIMIE文献大全
  • Inhibitors of specific ceramide synthases.

    abstract：:Ceramide synthases (CerSs) are key enzymes in the biosynthesis of ceramides and display a group of at least six different isoenzymes (CerS1-6). Ceramides itself are bioactive molecules. Ceramides with different N-acyl side chains (C(14:0)-Cer - C(26:0)-Cer) possess distinct roles in cell signaling. Therefore, the sele...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2011.09.007

    authors： Schiffmann S,Hartmann D,Fuchs S,Birod K,Ferreiròs N,Schreiber Y,Zivkovic A,Geisslinger G,Grösch S,Stark H

    更新日期：2012-02-01 00:00:00

  • Fibrillation properties of human α₁-acid glycoprotein.

    abstract：:Human α(1)-acid glycoprotein (AGP) is a positive acute phase plasma protein containing two disulfide bridges. Structural studies have shown that under specific conditions AGP undergoes aggregation. In this study, we analysed the nature of AGP's aggregates formed under reducing and non-reducing conditions at pH 5.5 and...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2012.09.005

    authors： Scirè A,Baldassarre M,Galeazzi R,Tanfani F

    更新日期：2013-02-01 00:00:00

  • The biosynthesis of highly branched N-glycans: studies on the sequential pathway and functional role of N-acetylglucosaminyltransferases I, II, III, IV, V and VI.

    abstract：:At least 6 N-acetylglucosaminyltransferases (GlcNAc-T I, II, III, IV, V and VI) are involved in initiating the synthesis of the various branches found in complex asparagine-linked oligosaccharides (N-glycans), as indicated below: GlcNAc beta 1-6 GlcNAc-T V GlcNAc beta 1-4 GlcNAc-T VI GlcNAc beta 1-2Man alpha 1-6 GlcNA...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/0300-9084(88)90289-1

    authors： Brockhausen I,Narasimhan S,Schachter H

    更新日期：1988-11-01 00:00:00

  • Non-canonical substrates of aminoacyl-tRNA synthetases: the tRNA-like structure of brome mosaic virus genomic RNA.

    abstract：:A 3-D model of the tyrosylable tRNA-like domain of the genomic brome mosaic virus RNAs was built by computer modelling based on solution probing of the molecule with different chemical and enzymatic reagents. This model encompasses four major structural domains, including two peculiar substructures oriented perpendicu...

    journal_title：Biochimie

    pub_type： 杂志文章,评审

    doi：10.1016/0300-9084(93)90014-j

    authors： Felden B,Florentz C,Westhof E,Giegé R

    更新日期：1993-01-01 00:00:00

  • Effect of C-terminal truncation on the molecular chaperone function and dimerization of Escherichia coli trigger factor.

    abstract：:To examine the role of the C-terminal domain in the chaperone function of trigger factor (TF), a number of truncation mutants were constructed, namely: TF419, TF389, TF380, TF360, TF344, and TF251, in which the C-terminal 13, 43, 52, 72, 88 residues or the entire C-domain were deleted, respectively. Co-expression of m...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2005.11.006

    authors： Zeng LL,Yu L,Li ZY,Perrett S,Zhou JM

    更新日期：2006-06-01 00:00:00

  • Light induced H2 evolution in a hydrogenase-TiO2 particle system by direct electron transfer or via rhodium complexes.

    abstract：:Three different hydrogenases (isolated from Clostridium pasteurianum, Desulfovibrio desulfuricans strain Norway 4 and D. baculatus 9974) added to a suspension of TiO2 (anatase) powder are able to catalyze H2 evolution under band gap illumination of the semiconducting particles, and in the presence of EDTA or methanol ...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/s0300-9084(86)81086-0

    authors： Cuendet P,Rao KK,Grätzel M,Hall DO

    更新日期：1986-01-01 00:00:00

  • Antihyperglycemic effect of fraxetin on hepatic key enzymes of carbohydrate metabolism in streptozotocin-induced diabetic rats.

    abstract：:Epidemiological studies have demonstrated that the diabetes mellitus is a serious health burden for both governments and healthcare providers. The present study was hypothesized to evaluate the antihyperglycemic potential of fraxetin by determining the activities of key enzymes of carbohydrate metabolism in streptozot...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2013.06.013

    authors： Murali R,Srinivasan S,Ashokkumar N

    更新日期：2013-10-01 00:00:00

  • Involvement of plasma membrane proteins in plant defense responses. Analysis of the cryptogein signal transduction in tobacco.

    abstract：:Cryptogein, a 98 amino acid protein secreted by the fungus Phytophthora cryptogea, induces a hypersensitive response and systemic acquired resistance in tobacco plants (Nicotiana tabacum var Xanthi). The mode of action of cryptogein has been studied using tobacco cell suspensions. The recognition of this elicitor by a...

    journal_title：Biochimie

    pub_type： 杂志文章,评审

    doi：10.1016/s0300-9084(99)80123-0

    authors： Lebrun-Garcia A,Bourque S,Binet MN,Ouaked F,Wendehenne D,Chiltz A,Schäffner A,Pugin A

    更新日期：1999-06-01 00:00:00

  • Mutations in ribosomal proteins L7/L12 perturb EF-G and EF-Tu functions.

    abstract：:In vitro cycling rates of E. coli ribosomes and of elongation factors EF-Tu and EF-G have been obtained and these are compatible with translation rates in vivo. We show that the rate of translocation is faster than 50 s-1 and therefore that the EF-G function is not a rate limiting step in protein synthesis. The in viv...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/0300-9084(88)90244-1

    authors： Bilgin N,Kirsebom LA,Ehrenberg M,Kurland CG

    更新日期：1988-05-01 00:00:00

  • S100 and S100 fused-type protein families in epidermal maturation with special focus on S100A3 in mammalian hair cuticles.

    abstract：:Epithelial Ca(2+)-regulation, which governs cornified envelope formation in the skin epidermis and hair follicles, closely coincides with the expression of S100A3, filaggrin and trichohyalin, and the post-translational modification of these proteins by Ca(2+)-dependent peptidylarginine deiminases. This review summariz...

    journal_title：Biochimie

    pub_type： 杂志文章,评审

    doi：10.1016/j.biochi.2011.05.028

    authors： Kizawa K,Takahara H,Unno M,Heizmann CW

    更新日期：2011-12-01 00:00:00

  • Characterization of conserved microRNAs from five different cucurbit species using computational and experimental analysis.

    abstract：:MicroRNAs (miRNAs) are ∼21 nt non-coding small RNAs which regulate gene expression at the post-transcriptional level in plants and animals. Until recently, only limited numbers of miRNAs were identified in Cucurbitaceae, a large flowering plant family. In this study, 220 potential miRNA candidates were identified from...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2014.03.002

    authors： Hu J,Sun L,Zhu Z,Zheng Y,Xiong W,Ding Y

    更新日期：2014-07-01 00:00:00

  • [Modes of action of a shortening system for erucic acid at the mitochondrial level of rat liver].

    abstract：:In this work, were studied the conditions of erucic acid (cis-docosenoic, n-9) shortening by using Rat liver mitochondrial preparations which were incubated in vitro with [14-14C] erucic acid (22:1), with inhibitors of the respiratory chain (rotenone, cyanide) or not, with activators of either the shortening reaction ...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/s0300-9084(82)80352-0

    authors： Clouet P,Henninger C,Bezard J

    更新日期：1982-10-01 00:00:00

  • Calcium, magnesium and the conformation of parvalbumin during muscular activity.

    abstract：:The conformation of perch parvalbumin in the Ca-, Mg- and metal-free state was studied by intrinsic fluorescence, trypsin susceptibility, thiol titration and circular dichroism. The data reveal that Ca-parvalbumin has a more compact structure than the metal-free protein, with a high alpha-helical content and a buried ...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/s0300-9084(79)80157-1

    authors： Cox JA,Winge DR,Stein EA

    更新日期：1979-01-01 00:00:00

  • Heteroarene-fused anthraquinone derivatives as potential modulators for human aurora kinase B.

    abstract：:The quest for effective anticancer therapeutics continues to be extensively pursued. Over the past century, several drugs have been developed, however, a majority of these drugs have a poor therapeutic index and increased toxicity profile. Hence, there still exists ample opportunity to discover safe and effective anti...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2020.12.024

    authors： Singh M,Malhotra L,Haque MA,Kumar M,Tikhomirov A,Litvinova V,Korolev AM,Ethayathulla AS,Das U,Shchekotikhin AE,Kaur P

    更新日期：2021-01-06 00:00:00

  • Comparison of solution structure of free and complexed lac operator by molecular modelling with NMR constraints.

    abstract：:The structure difference between the free operator of the lac system d(GCTCACAAT).d(ATTGTGAGC) and the same operator complexed to the headpiece of the lac repressor has been investigated by 2-D-1H NMR spectroscopy in conjunction with molecular modelling in internal coordinates (JUMNA). The free and complexed operator ...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/0300-9084(94)90006-x

    authors： Gincel E,Lancelot G,Maurizot JC,Thuong NT,Vovelle F

    更新日期：1994-01-01 00:00:00

  • Deimination of glycogen phosphorylase b by peptidylarginine deiminase. Influence on the kinetical characteristics and dimer-tetramer transition.

    abstract：:The kinetics of the native glycogen phosphorylase b from rabbit skeletal muscle and of the enzyme specifically deiminated by peptidylarginine deiminase have been studied. One arginine residue per phosphorylase b monomer is transformed into citrulline after 3 h of incubation with peptidylarginine deiminase. The maximal...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/0300-9084(96)82188-2

    authors： Eronina TB,Livanova NB,Chebotareva NA,Kurganov BI,Luo S,Graves DJ

    更新日期：1996-01-01 00:00:00

  • Crystal structure of the NADP-dependent mannitol dehydrogenase from Cladosporium herbarum: Implications for oligomerisation and catalysis.

    abstract：:The ascomycete Cladosporium herbarum is a prominent fungal inducer of Type I allergy. The only major allergen identified so far is Cla h 8, a NADP-dependent mannitol dehydrogenase (MtDH). MtDH, a cytoplasmic protein of 28.5kDa, belongs to the Short chain Dehydrogenases/Reductases (SDR), acting as a NADP-dependent oxid...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2010.04.012

    authors： Nüss D,Goettig P,Magler I,Denk U,Breitenbach M,Schneider PB,Brandstetter H,Simon-Nobbe B

    更新日期：2010-08-01 00:00:00

  • MicroRNA-590 attenuates lipid accumulation and pro-inflammatory cytokine secretion by targeting lipoprotein lipase gene in human THP-1 macrophages.

    abstract：BACKGROUND:Accumulating evidence suggests that microRNA-590 (miR-590) has protective effects on cardiovascular diseases, but the mechanism is unknown. Interestingly, previous studies from our laboratory and others have shown that macrophage-derived lipoprotein lipase (LPL) might accelerate atherosclerosis by promoting ...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2014.08.003

    authors： He PP,Ouyang XP,Tang YY,Liao L,Wang ZB,Lv YC,Tian GP,Zhao GJ,Huang L,Yao F,Xie W,Tang YL,Chen WJ,Zhang M,Li Y,Wu JF,Peng J,Liu XY,Zheng XL,Yin WD,Tang CK

    更新日期：2014-11-01 00:00:00

  • Glutathione modulation during sensitization as well as challenge phase regulates airway reactivity and inflammation in mouse model of allergic asthma.

    abstract：:Glutathione, being a major intracellular redox regulator has been shown to be implicated in regulation of airway reactivity and inflammation. However, no study so far has investigated the effect of glutathione depletion/repletion during sensitization and challenge phases separately, which could provide an important in...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2014.04.001

    authors： Nadeem A,Siddiqui N,Alharbi NO,Alharbi MM,Imam F,Sayed-Ahmed MM

    更新日期：2014-08-01 00:00:00

  • The human testis determining factor SRY: a new member of the HMG box protein family.

    abstract：:The product of the sex-determining gene SRY is a member of the HMG box containing protein superfamily. The HMG box is a DNA-binding domain of about 80 amino acids shared by many proteins with diverse functions. It seems that the functions of the full length protein are restricted to the HMG box but their molecular bas...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/0300-9084(94)90033-7

    authors： Soullier S,Poulat F,Boizet-Bonhoure B,Calas B,Bennes R,Heitz F,Berta P

    更新日期：1994-01-01 00:00:00

  • The heart-protective mechanism of nitronyl nitroxide radicals on murine viral myocarditis induced by CVB3.

    abstract：:Our previous researches showed that nitronyl nitroxyl derivatives, NNP and NNVP were good anti-oxidants and provided radioprotective effects in C6 cells. The objective of the present study is to investigate the possible antiviral effects and underlying pharmacological of the two nitronyl nitroxide radicals against CVB...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2012.05.015

    authors： Wang H,Gao P,Jing L,Qin X,Sun X

    更新日期：2012-09-01 00:00:00

  • Differential contributions of recognition factors of two plant lectins -Amaranthus caudatus lectin and Arachis hypogea agglutinin, reacting with Thomsen-Friedenreich disaccharide (Galbeta1-3GalNAcalpha1-Ser/Thr).

    abstract：:Previous reports on the carbohydrate specificities of Amaranthus caudatus lectin (ACL) and peanut agglutinin (PNA, Arachis hypogea) indicated that they share the same specificity for the Thomsen-Friedenreich (T(alpha), Galbeta1-3GalNAcalpha1-Ser/Thr) glycotope, but differ in monosaccharide binding--GalNAc>Gal (inactiv...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2008.08.001

    authors： Wu AM,Wu JH,Yang Z,Singh T,Goldstein IJ,Sharon N

    更新日期：2008-11-01 00:00:00

  • Fine tuning gene expression through short DNA-protein binding cycles.

    abstract：:Certain transcription factors have recently been shown to interact with DNA in living cells, through very short binding cycles, contrasting with the data previously obtained in vitro, and with the view of a stepwise building of transcription initiation complexes. These short cycles are triggered by active dissociation...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2009.03.022

    authors： Michel D

    更新日期：2009-07-01 00:00:00

  • Toxicity, binding and internalization of the pea-A1b entomotoxin in Sf9 cells.

    abstract：:PA1b (Pea Albumin 1b) is a peptide toxin lethal for certain insects. This paper shows that the cultured insect cells Sf9 are sensitive to the toxin and display a high-affinity binding site for PA1b. Mammalian cells are not sensitive and no binding activity was detected. Signs of apoptosis of the Sf9 cells were observe...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2007.07.021

    authors： Rahioui I,Laugier C,Balmand S,Da Silva P,Rahbe Y,Gressent F

    更新日期：2007-12-01 00:00:00

  • Overproduction of the membrane-bound components of the histidine permease from Salmonella typhimurium: identification of the M protein.

    abstract：:The periplasmic histidine permease of Salmonella typhimurium is composed of a soluble histidine-binding protein and three membrane-bound components. These latter are produced in very small amounts and only two, the Q and the P protein, have been previously identified. This paper describes the construction of a plasmid...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/s0300-9084(85)80242-x

    authors： Ames GF,Nikaido K,Hobson A,Malcolm B

    更新日期：1985-01-01 00:00:00

  • Inactivation of proteases and esterases by halomethylated derivatives of dihydrocoumarins.

    abstract：:A series of halomethylated derivatives of dihydrocoumarins has been found to inhibit irreversibly proteases and esterases. alpha-Chymotrypsin, subtilish, elastase are rapidly inactivated in the presence of these compounds, while trypsin, kallicrein, papain are inhibited more slowly. Esterases like acetylcholinesterase...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/s0300-9084(77)80140-5

    authors： Béchet JJ,Dupaix A,Roucous C,Bonamy AM

    更新日期：1977-01-01 00:00:00

  • Phage resistance in lactic acid bacteria.

    abstract：:The interactions between lactic acid bacteria and their phages are commercially significant. Current research has focused on the elucidation of the mechanisms and genetics of phage resistance. Phage resistance genes have been linked to plasmid DNA for Streptococcus lactis and Streptococcus cremoris, and preliminary st...

    journal_title：Biochimie

    pub_type： 杂志文章,评审

    doi：10.1016/0300-9084(88)90215-5

    authors： Sanders ME

    更新日期：1988-03-01 00:00:00

  • Use of a new integrational vector to investigate compartment-specific expression of the Bacillus subtilis spoIIM gene.

    abstract：:The product of the spoIIM gene of Bacillus subtilis is required for complete septum migration and forespore engulfment during sporulation. To investigate whether expression of spoIIM is required in the forespore compartment of the sporangium, we have constructed a new integrational vector, pKSV7, which contains temper...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/0300-9084(92)90143-3

    authors： Smith K,Youngman P

    更新日期：1992-07-01 00:00:00

  • Sensitive to apoptosis gene protein regulates ionizing radiation-induced apoptosis.

    abstract：:Organisms exposed to ionizing radiation (IR) undergo increases in the production of reactive oxygen species (ROS), which are determinant components in the induction of apoptosis. Sensitive to apoptosis gene (SAG) encodes a redox-inducible and apoptosis-protective antioxidant protein. This report demonstrates that the ...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2010.09.020

    authors： Kim SY,Yang ES,Lee YS,Lee J,Park JW

    更新日期：2011-02-01 00:00:00

  • Efficient adenoviral transduction of 3T3-F442A preadipocytes without affecting adipocyte differentiation.

    abstract：:The preadipocyte cell lines 3T3-L1 and 3T3-F442A are widely used to study the cellular mechanisms of preadipocyte differentiation and mature adipocyte functions. However, transfection with naked DNA is inefficient in these cell lines. Adenoviral gene transfer is a powerful technique to induce high levels of transgene ...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2005.05.010

    authors： Béréziat V,Moritz S,Klonjkowski B,Decaudain A,Auclair M,Eloit M,Capeau J,Vigouroux C

    更新日期：2005-11-01 00:00:00