Mutations in ribosomal proteins L7/L12 perturb EF-G and EF-Tu functions.

Abstract:

:In vitro cycling rates of E. coli ribosomes and of elongation factors EF-Tu and EF-G have been obtained and these are compatible with translation rates in vivo. We show that the rate of translocation is faster than 50 s-1 and therefore that the EF-G function is not a rate limiting step in protein synthesis. The in vivo phenotype of some L7/L12 mutants could be accounted for by perturbed EF-Tu as well as EF-G functions. The S12 mutants that we studied were, in contrast, only perturbed in their EF-Tu function, while their EF-G interaction was not impaired in relation to wild type ribosomes.

journal_name

Biochimie

journal_title

Biochimie

authors

Bilgin N,Kirsebom LA,Ehrenberg M,Kurland CG

doi

10.1016/0300-9084(88)90244-1

subject

Has Abstract

pub_date

1988-05-01 00:00:00

pages

611-8

issue

5

eissn

0300-9084

issn

1638-6183

pii

0300-9084(88)90244-1

journal_volume

70

pub_type

杂志文章
  • The pericyte secretome: Potential impact on regeneration.

    abstract::Personalized and regenerative medicine is an emerging therapeutic strategy that is based on cell biology and biomedical engineering used to develop biological substitutes to maintain normal function or restore damaged tissues and organs. The secretory capacities of different cell types are now explored as such possibl...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/j.biochi.2018.04.015

    authors: Gaceb A,Barbariga M,Özen I,Paul G

    更新日期:2018-12-01 00:00:00

  • Effect of the thermal variation on the molecular weight estimation of the acid phosphatase from Physarum polycephalum.

    abstract::A Binding Protein (BP) of nearly 95,000 daltons interacts with the acid phosphatase at the permissive temperature, elevating its molecular weight from 48,000 +/- 2,000 to 136,000 +/- 7,000 in CL and CHR strains of Physarum polycephalum plasmodia. The same molecule has not been observed in the extracts of M3C VIII and ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(83)80160-6

    authors: Demirtas H

    更新日期:1983-06-01 00:00:00

  • L-lactate oxidase from Aerococcus viridans crystallized as an octamer. Preliminary X-ray studies.

    abstract::Crystals of flavoenzyme L-lactate oxidase from Aerococcus viridans (LOX) have been obtained that diffract to 3.0 A resolution (P2(1)2(1)2(1), a = 118.4 A, b = 138.4 A, c = 194.6 A). Crystallographic studies suggest that the enzyme may exist as an octameric form with non-crystallographic two- and four-fold axes in the ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(98)80072-2

    authors: Morimoto Y,Yorita K,Aki K,Misaki H,Massey V

    更新日期:1998-04-01 00:00:00

  • [Typical tripeptide sequences of collagen: quantitative conformation study].

    abstract::Theoretical and experimental investigations on the conformations of synthetic polytripeptides, with Gly-R2-R3 general repetitive sequence, are of great interest for the comprehension of the factors which contribute to the triple helix stabilization. Using appropriate molecules as models, we have investigated by the PC...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:

    authors: Cabrol D,Broch H,Vasilescu D

    更新日期:1981-11-01 00:00:00

  • Reciprocal effects of 2-fluoro-2-deoxy-D-glucose and glucose on their metabolism in Saccharomyces cerevisiae studied by multi-nuclear NMR spectroscopy.

    abstract::The effects of various concentrations of 2-fluoro-2-deoxy-D-glucose (FDG) on the aerobic metabolism of glucose and the reciprocal effect of glucose on the metabolism of FDG in glucose-grown repressed Saccharomyces cerevisiae cells were studied at 30 degrees C in a standard pyrophosphate medium containing 5 x 10(7) cel...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(96)88129-6

    authors: Tran-Dinh S,Courtois A,Wietzerbin J,Bouet F,Herve M

    更新日期:1995-01-01 00:00:00

  • Characterization of ribonuclease H activities present in two cell-free protein synthesizing systems, the wheat germ extract and the rabbit reticulocyte lysate.

    abstract::Experimental evidence accumulated to date by several research groups indicates that antisense oligodeoxynucleotides targeted against messenger RNA (mRNA) sequences located downstream of the initiation codon fail to inhibit the translation of this mRNA unless the hybrid is cleaved by RNase H. It has previously been sho...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(93)90032-n

    authors: Cazenave C,Frank P,Büsen W

    更新日期:1993-01-01 00:00:00

  • An oviduct-specific and enhancer-like element resides at about -3000 in the chicken ovalbumin gene.

    abstract::The chicken ovalbumin (Ov) gene is one of the best models to study tissue-specific gene regulation because it is only expressed in the oviduct. In this paper, a tissue-specific element was characterized by 5'-flanking region deletion in combination with in vivo gene electroporation (EP). Plasmids with varying lengths ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2006.06.015

    authors: Park HM,Sanders MM,Suzuki T,Muramatsu T

    更新日期:2006-12-01 00:00:00

  • A region in the C-terminal domain of ribosomal protein SA required for binding of SA to the human 40S ribosomal subunit.

    abstract::The human ribosomal protein SA, known also as a precursor of the cell-surface laminin receptor, LAMR, is a protein of the 40S ribosomal subunit. It is homologous to eubacterial ribosomal protein S2p, but has a eukaryote-specific C-terminal domain (CTD) that is responsible in LAMR for the binding of laminin as well as ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2010.12.005

    authors: Malygin AA,Babaylova ES,Loktev VB,Karpova GG

    更新日期:2011-03-01 00:00:00

  • Phospholipases A2 in ocular homeostasis and diseases.

    abstract::Phospholipases A(2) (PLA(2)s) and its generation of second messengers play an important role in signal transduction, cell proliferation, cell survival and gene expression. At low concentrations mediators of PLA(2) activity have a variety of physiological effects whereas high levels of PLA(2) and its metabolites are ge...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/j.biochi.2010.04.007

    authors: Wang J,Kolko M

    更新日期:2010-06-01 00:00:00

  • LysK, the enzyme lysing Staphylococcus aureus cells: specific kinetic features and approaches towards stabilization.

    abstract::LysK, the enzyme lysing cells of Staphylococcus aureus, can be considered as perspective antimicrobial agent. Knowledge of LysK properties and behavior would allow optimizing conditions of its storage as well as formulating strategy towards its stabilization. Reaction of LysK with substrate (suspension of autoclaved S...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2010.01.026

    authors: Filatova LY,Becker SC,Donovan DM,Gladilin AK,Klyachko NL

    更新日期:2010-05-01 00:00:00

  • Metabolic functions of peroxisomes in health and disease.

    abstract::Peroxisomes are subcellular organelles which are present in virtually every eukaryotic cell and catalyze a large number of metabolic functions. The importance of peroxisomes for humans is stressed by the existence of a large group of genetic diseases in which either the biogenesis of peroxisomes is impaired or one of ...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/j.biochi.2013.08.022

    authors: Wanders RJ

    更新日期:2014-03-01 00:00:00

  • Conformational features of bradykinin. A circular dichroism study of some peptide fragments and structural analogues of bradykinin.

    abstract::The vasoactive hormone bradykinin, its N-and C-terminal fragments and some structural analogues were studied by Circular Dichroism. Conformational features of the peptide can be detected by comparative analysis of the various CD spectra recorded as a function of aqueous pH, solvent and temperature. It is shown that th...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(79)80316-8

    authors: Lintner K,Fermandjian S,Regoli D

    更新日期:1979-01-01 00:00:00

  • Peptide synthesis by recombinant Fasciola hepatica cathepsin L1.

    abstract::Synthesis of the tripeptide Z-Phe-Arg-SerNH2 has been accomplished by a recombinant cysteine protease, cathepsin L1 from liver fluke (Fasciola hepatica), using Z-Phe-Arg-OMe as acyl acceptor and SerNH2 as nucleophile in 0.1 M ammonium acetate pH 9.0-12.5% v/v acetonitrile at 37 degrees C. LC-MS detection indicated tri...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2005.06.004

    authors: Ruth DM,McMahon G,O'Fágáin C

    更新日期:2006-01-01 00:00:00

  • Denitrification and nitrite reduction: Pseudomonas aeruginosa nitrite-reductase.

    abstract::Present knowledge of the different enzymatic steps of the denitrification chains in various bacteria, particularly Paracoccus denitrificans and Pseudomonas aeruginosa has been briefly reviewed. The question whether nitric oxide (NO), nitrous oxide (N2O) and other nitrogen derivatives are obligatory intermediates has b...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/0300-9084(84)90005-1

    authors: Henry Y,Bessières P

    更新日期:1984-04-01 00:00:00

  • Cysteine proteinase content of rat muscle lysosomes. Evidence for an unusual proteinase activity.

    abstract::Lysosomal cysteine proteinases were fractionated from partially purified rat muscle lysosomes. By gel filtration on Sephadex G75, cathepsin D was separated from two thiol-requiring proteolytic fractions of Mr 25 000 and 55 000, respectively. By chromatofocusing, the first fraction (Mr = 25 000) was resolved into three...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(84)90114-7

    authors: Obled A,Ouali A,Valin C

    更新日期:1984-09-01 00:00:00

  • Involvement of autotaxin/lysophosphatidic acid signaling in obesity and impaired glucose homeostasis.

    abstract::Autotaxin (ATX) is a secreted lysophospholipase D involved in synthesis of lysophosphatidic acid (LPA), a phospholipid growth factor acting via specific receptors (LPA1R to LPA6R) and involved in several pathologies including obesity. ATX is secreted by adipocytes and contributes to circulating LPA. ATX expression is ...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/j.biochi.2013.04.010

    authors: Rancoule C,Dusaulcy R,Tréguer K,Grès S,Attané C,Saulnier-Blache JS

    更新日期:2014-01-01 00:00:00

  • Metabolism of glucose, glutamine, long-chain fatty acids and ketone bodies by lungs of the rat.

    abstract::The maximum activities of some key enzymes of metabolism were studied in lungs of fed and 48-h-starved rats. The maximum activity of hexokinase in the lung is similar to that of other tissues of the body, but lower than that of phosphorylase and 6-phosphofructokinase. High activities of glucose-6-phosphate dehydrogena...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(91)90023-t

    authors: Ardawi MS

    更新日期:1991-05-01 00:00:00

  • Identification of multiple antimicrobial peptides from the skin of fine-spined frog, Hylarana spinulosa (Ranidae).

    abstract::In this study, peptidomics and genomics analyses were used to study antimicrobial peptides from the skin of Hylarana spinulosa. Twenty-nine different antimicrobial peptide precursors were characterized from the skin of H. spinulosa, which produce 23 mature antimicrobial peptides belonging to 12 different families. To ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2013.09.002

    authors: Yang X,Hu Y,Xu S,Hu Y,Meng H,Guo C,Liu Y,Liu J,Yu Z,Wang H

    更新日期:2013-12-01 00:00:00

  • Intracellular phospholipase activity in rat heart: comparison between endogenous and exogenous substrates.

    abstract::Two methods were used to estimate the intracellular phospholipase activity in rat heart: one using exogenous radioactive substrate dispersed as unilamellar vesicles; the other using endogenous membrane hydrolysis and subsequent phospholipid and lysophospholipid separation by high-performance liquid chromatography and ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(87)90049-6

    authors: Termine E,Léonardi J,Lafont H,Nalbone G

    更新日期:1987-03-01 00:00:00

  • Purification and characterization of extracellular pectate lyase from Bacillus subtilis.

    abstract::Bacillus subtilis strain SO113 secretes a pectate lyase which is produced during the exponential death phase of growth, just before sporulation. This extracellular pectate lyase, which produces unsaturated products from polygalacturonate, was purified 35-fold from the culture supernatant of Bacillus subtilis by a CM S...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(90)90053-j

    authors: Nasser W,Chalet F,Robert-Baudouy J

    更新日期:1990-09-01 00:00:00

  • Identification and biochemical characterization of Rap2C, a new member of the Rap family of small GTP-binding proteins.

    abstract::The Rap family of small GTP-binding proteins is composed by four different members: Rap1A, Rap1B, Rap2A and Rap2B. In this work we report the identification and characterization of a fifth member of this family of small GTPases. This new protein is highly homologous to Rap2A and Rap2B, binds labeled GTP on nitrocellul...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2005.08.007

    authors: Paganini S,Guidetti GF,Catricalà S,Trionfini P,Panelli S,Balduini C,Torti M

    更新日期:2006-03-01 00:00:00

  • Expression of eukaryotic glycosyltransferases in the yeast Pichia pastoris.

    abstract::The methylotrophic yeast Pichia pastoris is often used as an organism for the heterologous expression of proteins and has been used already for production of a number of glycosyltransferases involved in the biosynthesis of N- and O-linked oligosaccharides. In our recent studies, we have examined the expression in P. p...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(03)00072-5

    authors: Bencúrová M,Rendić D,Fabini G,Kopecky EM,Altmann F,Wilson IB

    更新日期:2003-03-01 00:00:00

  • An interfacial and comparative in vitro study of gastrointestinal lipases and Yarrowia lipolytica LIP2 lipase, a candidate for enzyme replacement therapy.

    abstract::Lipolytic activities of Yarrowia lipolytica LIP2 lipase (YLLIP2), human pancreatic (HPL) and dog gastric (DGL) lipases were first compared using lecithin-stabilized triacylglycerol (TAG) emulsions (Intralipid) at various pH and bile salt concentrations. Like DGL, YLLIP2 was able to hydrolyze TAG droplets covered by a ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2014.03.004

    authors: Bénarouche A,Point V,Carrière F,Cavalier JF

    更新日期:2014-07-01 00:00:00

  • [Cyclic nucleotide phosphodiesterase in vertebrates].

    abstract::The cellular concentration of cyclic nucleotides is largely dependent upon the activity of the enzymatic system responsible for their degradation: cyclic nucleotide phosphodiesterase. This enzymatic system thus plays a crucial role in the regulation of the multiple functions which are modulated by cyclic nucleotides i...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(84)90203-7

    authors: Nemoz G,Prigent AF

    更新日期:1984-02-01 00:00:00

  • Purification and characterization of a germ cell-specific form of elongation factor 1 alpha (EF-1 alpha) from Xenopus laevis.

    abstract::Elongation factor 1 alpha (EF-1 alpha) was purified to homogeneity from full-grown oocytes of Xenopus laevis. This protein is encoded by a gene previously shown to be expressed in male and female germ cells, and repressed in somatic cells. The purified protein was identified with EF-1 alpha on criteria of molecular ma...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(91)90011-o

    authors: Morales J,Mulner-Lorillon O,Denis H,Bellé R

    更新日期:1991-09-01 00:00:00

  • Recognition of cell surface acceptors by two human alpha-2,6-sialyltransferases produced in CHO cells.

    abstract::The action of sialyltransferases (STs) on cell surface glycoconjugates is a key process in shaping cell phenotype in a variety of cells mostly involved in migratory and adhesive pathways. The factors determining cell-specific pattern of glycosylation are so far poorly understood. Most STs are resident proteins of the ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(03)00080-4

    authors: Donadio S,Dubois C,Fichant G,Roybon L,Guillemot JC,Breton C,Ronin C

    更新日期:2003-03-01 00:00:00

  • Effect of dietary polyunsaturated fatty acids on the expression of peroxisomal ABC transporters.

    abstract::Peroxisomal ABC transporters encoded by the ABCD genes are thought to participate in the import of specific fatty acids in the peroxisomal matrix. ABCD1 deficiency is associated with X-linked adrenoleukodystrophy (X-ALD), the most frequent peroxisomal disorder which is characterized by the accumulation of saturated ve...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2008.05.022

    authors: Leclercq S,Skrzypski J,Courvoisier A,Gondcaille C,Bonnetain F,André A,Chardigny JM,Bellenger S,Bellenger J,Narce M,Savary S

    更新日期:2008-10-01 00:00:00

  • Crystal structure of the NADP-dependent mannitol dehydrogenase from Cladosporium herbarum: Implications for oligomerisation and catalysis.

    abstract::The ascomycete Cladosporium herbarum is a prominent fungal inducer of Type I allergy. The only major allergen identified so far is Cla h 8, a NADP-dependent mannitol dehydrogenase (MtDH). MtDH, a cytoplasmic protein of 28.5kDa, belongs to the Short chain Dehydrogenases/Reductases (SDR), acting as a NADP-dependent oxid...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2010.04.012

    authors: Nüss D,Goettig P,Magler I,Denk U,Breitenbach M,Schneider PB,Brandstetter H,Simon-Nobbe B

    更新日期:2010-08-01 00:00:00

  • The nicotinamide subsite of glyceraldehyde-3-phosphate dehydrogenase studied by site-directed mutagenesis.

    abstract::Directed mutagenesis has been used to study the nicotinamide subsite of the glycolytic NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Residue Asn313 is involved together with the carboxyamide moiety of the nicotinamide ring in a complex network of hydrogen bonding interactions which fix the position o...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(90)90119-2

    authors: Corbier C,Mougin A,Mely Y,Adolph HW,Zeppezauer M,Gerard D,Wonacott A,Branlant G

    更新日期:1990-08-01 00:00:00

  • FhCaBP3: a Fasciola hepatica calcium binding protein with EF-hand and dynein light chain domains.

    abstract::A DNA sequence encoding a protein with predicted EF-hand and dynein light chain binding domains was identified in a Fasciola hepatica EST library. Sequence analysis of the encoded protein revealed that the most similar known protein was the Fasciola gigantica protein FgCaBP3 and so this newly identified protein was na...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2012.10.027

    authors: Banford S,Drysdale O,Hoey EM,Trudgett A,Timson DJ

    更新日期:2013-04-01 00:00:00