Abstract：
:Human α(1)-acid glycoprotein (AGP) is a positive acute phase plasma protein containing two disulfide bridges. Structural studies have shown that under specific conditions AGP undergoes aggregation. In this study, we analysed the nature of AGP's aggregates formed under reducing and non-reducing conditions at pH 5.5 and at relatively low temperatures. Thioflavin T and Congo red spectroscopic analyses indicated the presence of cross-β structures in both unreduced and reduced AGP aggregates. In these samples amyloid-like fibrils were detected by transmission electron microscopy. The fibrils are branched and bent and present in very large amount in reduced AGP. Kinetics of AGP fibrillation proceeds without a lag phase and the rate constants of cross-β formation are linearly dependent on AGP concentration and result higher under reducing conditions. The data suggest a possible downhill mechanism of polymerization with a first-order monomer concentration dependence. Bioinformatics tools highlighted an extended region that sheathes one side of the molecule containing aggregation-prone regions. Reducing conditions make the extended region less constricted, allowing greater exposure of aggregation-prone regions, thus explaining the higher propensity of AGP to aggregate and fibrillate.
journal_name
Biochimiejournal_title
Biochimieauthors
Scirè A,Baldassarre M,Galeazzi R,Tanfani Fdoi
10.1016/j.biochi.2012.09.005subject
Has Abstractpub_date
2013-02-01 00:00:00pages
158-66issue
2eissn
0300-9084issn
1638-6183pii
S0300-9084(12)00363-Xjournal_volume
95pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:The Ty1 retrotransposon of the yeast Saccharomyces cerevisiae is a long terminal repeat mobile genetic element that transposes through an RNA intermediate. Initiation of minus-strand and plus-strand DNA synthesis are two critical steps during reverse transcription of the retrotransposon genome. Initiation of minus-str...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(96)80013-7
更新日期：1996-01-01 00:00:00
abstract：:The oncogenic potential of the high-risk human papillomavirus (HPV) relies on the expression of genes specifying the E7 and E6 proteins. To investigate further the variation in oligomeric structure that has been reported for different E7 proteins, an HPV-18 E7 cloned from a Hispanic woman with cervical intraepithelial...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.02.028
更新日期：2010-07-01 00:00:00
abstract：:OURCIN is a tool to build expert systems, which was developed by a joint team from INRIA and SEMA. We emphasized developing on this system the ergonomic features which make Expert Systems a comfortable and interesting approach for knowledge engineering. ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(85)80267-4
更新日期：1985-05-01 00:00:00
abstract：:Glyoxalate is an effector of oxidative phosphorylation in isolated mitochondria : it slows down State 3 but does not affect State 4 respiration. This report presents the findings of our study on the mechanism of action of glyoxalate ; these findings are listed below. The inhibition of Stage 3 respiration by glyoxalate...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(75)80145-3
更新日期：1975-01-01 00:00:00
abstract：:The proton NMR data of a synthetic model of deoxy-myoglobin and hemoglobin show one that:(i) the ground state of the high spin iron (II) complex must be described by at least two electronic levels separated by ca.200 cm-1 and resulting from the removal of the degeneracy of the 5E level; (ii) this in-plane rhombic dist...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(82)80285-x
更新日期：1981-11-01 00:00:00
abstract：:The iron absorption by duodenal enterocytes is a key step of its homeostasis. But the control of this absorption is complex and cannot be fully explicated with present knowledge. In a global transcriptome approach, we identified 60 genes over-expressed in hemin (iron) overload in Caco-2 cells, an in vitro model of duo...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2008.01.002
更新日期：2008-04-01 00:00:00
abstract：:Over the years, imino proton exchange measurements have provided a description of base-pair opening and of properties of internal motions of nucleic acids such as the individual opening of base pairs and the insensitivity of base-pair lifetimes to the nature and stability of neighboring pairs. A recent determination o...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(97)86936-2
更新日期：1997-12-01 00:00:00
abstract：:The activation of the antitumor protein antibiotic neocarzinostatin (NCS) by the carboxyl radical CO-2, a one-electron donor obtained selectively from gamma-ray irradiation of nitrous oxide-saturated formate buffer, has been investigated in the presence and in the absence of DNA at pH 4.7 and pH 7.0. The reaction of N...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(84)80023-1
更新日期：1983-11-01 00:00:00
abstract：:Over the last couple of decades, the PPAR family of transcription factors has received much attention from the pharmaceutical industry due to their profound ability to improve glucose and lipid metabolism upon agonist activation. However, more recently the interest in these nuclear receptors has faded because several ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2016.10.017
更新日期：2017-05-01 00:00:00
abstract：:Stroke is a worldwide major cause of mortality and morbidity. Preclinical studies have identified over 1000 molecules with brain-protective properties. More than 200 clinical trials have evaluated neuroprotective candidates for ischemic stroke yet, to date almost all failed, leading to a re-analysis of treatment strat...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2015.06.005
更新日期：2016-01-01 00:00:00
abstract：:Purified chromatin isolated from lymphocytic cells derived from patients with acute leukemia, or other lymphoproliferative disorders has been compared with chromatin isolated from normal human lymphocytic cells by gel electrophoresis and differential gradient ultracentrifugation. Thermal denaturation studies showed hi...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(75)80307-5
更新日期：1975-01-01 00:00:00
abstract：:Starting from total pancreatic mRNAs, turkey pancreatic lipase (TPL) cDNA was synthesized by RT-PCR and cloned into the PGEM-T vector. Amino acid sequence of the TPL is compared to that of human pancreatic lipase (HPL). A 3-D structure model of TPL was built using the 3-D structure of HPL as template, given the high a...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2006.05.022
更新日期：2006-10-01 00:00:00
abstract：:We isolated and purified the main lipids of the rat sciatic nerve. After methanolysis, fatty acids were isolated and purified by thin layer chromatography, and studied by gas chromatography. C 16 and C 18 fatty acids are the most abundant. Among the long-chain fatty acids, arachidonic acid (20:4) is present in some li...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(78)80202-8
更新日期：1978-01-01 00:00:00
abstract：:Rhizopus oryzae lipase (ROL) was found to be a true lipase. This enzyme presents the interfacial activation phenomenon. The N-terminal amino acid sequence of ROL was compared to those of rhizopus lipases. Purified ROL possesses the same N-terminal sequence as the mature Rhizopus niveus lipase (RNL). This sequence was ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(01)01283-4
更新日期：2001-06-01 00:00:00
abstract：:A variety of species of GPDH undergo acylation at two of the four active cystein sites per mole of tetrameric enzyme. This reaction requires tightly bound NAD+, a situation restricted to two of the four NAD sites per tetramer. S leads to N acyl transfer from cysteins to lysine in the diacyl enzyme yields an inactive e...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(81)80177-0
更新日期：1981-02-01 00:00:00
abstract：:The interactions of 6-8 ring hairpin polyamides (PAs) with the minor groove of DNA have been investigated extensively. More recent studies of large antiviral PAs (14-20 rings) active against small DNA tumor viruses lead to questions regarding the extent to which the DNA binding behaviors of the well studied, smaller P...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2019.07.021
更新日期：2019-10-01 00:00:00
abstract：:A large part of higher eukaryotic genomes is transcribed into RNAs lacking any significant open reading frame. This "non-coding part" has been shown to actively contribute to regulating gene expression, but the mechanisms are largely unknown. Particularly instructive examples are provided by the dosage compensation sy...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2011.12.026
更新日期：2012-07-01 00:00:00
abstract：:Obesity prevalence continues to rise throughout the developed world, as a result of positive energy balance and reduced physical activity. At present, there is still a perception within the general community, and amongst some nutritionists, that eating multiple small meals spaced throughout the day is beneficial for w...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2015.07.025
更新日期：2016-05-01 00:00:00
abstract：BACKGROUND:The modest efficacy of available therapies for Hepatocellular carcinoma (HCC) indicates the need to develop novel therapeutic approaches. For the proteasome inhibitor Bortezomib (BZB), potentially attractive for HCC treatment, the mechanism of action is largely unknown. The BZB effect on E2Fs and the E2Fs co...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2015.02.015
更新日期：2015-05-01 00:00:00
abstract：:Split luciferase complementary assay has been used to investigate the effect of WD domain deletion on Apaf-1 oligomerization. Apaf-1 is an adaptor molecule in formation of apoptosome that activates caspase-9, an activation that is a key event in the mitochondrial cell death pathway. Structural studies suggest that nor...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.10.013
更新日期：2021-01-01 00:00:00
abstract：:The major pterin in Tetrahymena pyriformis, strain W, earlier suggested to be L-threo-biopterin and named ciliapterin [1] is now identified as D-threo-neopterin (D-monapterin). This is the first example of a natural D-monapterin. This compound was characterized by its chromatographic behavior, its fluorescence propert...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(91)90089-j
更新日期：1991-10-01 00:00:00
abstract：:Long term cadmium (Cd) exposure in occupational and general environments may give rise to kidney dysfunction. This effect is usually considered to be the critical effect, i. e. the effect that occurs at relatively low level of exposure. The present review focused on studies of the prevalence of cadmium-related kidney ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2009.06.014
更新日期：2009-10-01 00:00:00
abstract：:The considerable affinity of tissue transglutaminase for heparin was the basis for use of heparin-based affinity matrices for enzyme purification. Interaction of transglutaminase with heparin might mimic the physiological binding to membrane heparan sulfates, accounting for the limited but significant fraction of enzy...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2005.01.012
更新日期：2005-06-01 00:00:00
abstract：:The polysaccharide mixture obtained by hot water extraction of Aloe vahombe leaves is composed of at least four different paritally acetylated glucomannans which differ in molecular weight, glucose to mannose ratios and acetyl contents. Furthermore, one fraction contains a small but significant amount of protein which...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(86)80187-0
更新日期：1986-09-01 00:00:00
abstract：:We are reporting here an analysis of the chymotryptic peptides obtained from the tryptic heme-binding fragment of flavocytochrome b2 (cytochrome b2 core). These results completely establish the sequence of the 96 residue-long fragment, for which preliminary evidence has been published before [24]. We also report full ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(76)80437-3
更新日期：1976-01-01 00:00:00
abstract：:The thyroid hormones L-thyroxine and triiodo-L-thyronine have profound effects on postembryonic development of most vertebrates. Analysis of their action in mammals is vitiated by the exposure of the developing foetus to a number of maternal factors which do not allow one to specifically define the role of thyroid hor...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(99)80082-0
更新日期：1999-04-01 00:00:00
abstract：:The comparison of the amino acid sequences of 5 cysteine proteinases: papain, actinidin, rat cathepsins B and H and chicken cathepsin L, demonstrates a striking homology among their sequences. The N-terminal region (residues 1-70 in papain) and C-terminal region (residues 118-212 in papain) display the highest sequenc...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(88)90004-1
更新日期：1988-10-01 00:00:00
abstract：:Apoptosis is the physiological process by which unwanted cells in an organism are killed. Bcl-2, a membrane-bound cytoplasmic protein, and its close relative Bcl-xL, are both effective inhibitors of apoptosis induced by a wide variety of stimuli in many different cell types. In a previous study, we reported that suppr...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(97)82011-1
更新日期：1997-10-01 00:00:00
abstract：:Bikunin (Bk) is a Künitz-type serine proteinase inhibitor, which occurs in human plasma, mainly as covalent complexes with one or two of the three peptide heavy chains. The leading member of this glycoprotein family is inter-alpha-inhibitor (I alpha I), which consists of two heavy chains (H1 and H2) linked to Bk. Bk c...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(03)00066-x
更新日期：2003-01-01 00:00:00
abstract：:Many human conditions, including neurological diseases, atherosclerosis, cancer, diabetic complications and aging, are thought to be associated with oxidative stress (OS). The development of reliable and informative markers for the characterization of OS in humans is thus highly important. Various endogenous markers a...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2012.03.005
更新日期：2013-03-01 00:00:00
