Abstract:
:Starting from total pancreatic mRNAs, turkey pancreatic lipase (TPL) cDNA was synthesized by RT-PCR and cloned into the PGEM-T vector. Amino acid sequence of the TPL is compared to that of human pancreatic lipase (HPL). A 3-D structure model of TPL was built using the 3-D structure of HPL as template, given the high amino acid sequence homology between the two lipases. Based on this model, the enhanced interaction power of TPL, as compared to that of HPL, into a phosphatidylcholine monolayer film, could be explained. We concluded that an increase in the exposed hydrophobic residues on the surface of TPL would be responsible for an enhanced interaction with a lipidic interface.
journal_name
Biochimiejournal_title
Biochimieauthors
Fendri A,Frikha F,Miled N,Gargouri Ydoi
10.1016/j.biochi.2006.05.022subject
Has Abstractpub_date
2006-10-01 00:00:00pages
1401-7issue
10eissn
0300-9084issn
1638-6183pii
S0300-9084(06)00106-4journal_volume
88pub_type
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