Cloning and molecular modelling of turkey pancreatic lipase: structural explanation of the increased interaction power with lipidic interface.

Abstract:

:Starting from total pancreatic mRNAs, turkey pancreatic lipase (TPL) cDNA was synthesized by RT-PCR and cloned into the PGEM-T vector. Amino acid sequence of the TPL is compared to that of human pancreatic lipase (HPL). A 3-D structure model of TPL was built using the 3-D structure of HPL as template, given the high amino acid sequence homology between the two lipases. Based on this model, the enhanced interaction power of TPL, as compared to that of HPL, into a phosphatidylcholine monolayer film, could be explained. We concluded that an increase in the exposed hydrophobic residues on the surface of TPL would be responsible for an enhanced interaction with a lipidic interface.

journal_name

Biochimie

journal_title

Biochimie

authors

Fendri A,Frikha F,Miled N,Gargouri Y

doi

10.1016/j.biochi.2006.05.022

subject

Has Abstract

pub_date

2006-10-01 00:00:00

pages

1401-7

issue

10

eissn

0300-9084

issn

1638-6183

pii

S0300-9084(06)00106-4

journal_volume

88

pub_type

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