Amphibian metamorphosis as a model for studying the developmental actions of thyroid hormone.

abstract：:Nephropathy is one of the most frequent complications of chronic diabetes. The main reason for nephropathy despite being hyperglycemia, but it progresses even after good glycemic control has been achieved in diabetic patients. The effects of prior exposure to high blood glucose conditions depend upon the severity and ...

journal_title：Biochimie

authors： Kushwaha K,Sharma S,Gupta J

更新日期：2020-03-01 00:00:00

abstract：:The combination of cDNA and RNA sequencing techniques has enabled determination of the complete sequence of one of the mRNAs coding for the precursor of the small subunit of ribulose bisphosphate carboxylase of Nicotiana sylvestris. In this 898-nucleotide-long mRNA, 540 nucleotides code for the entire 180-amino-acid-l...

journal_title：Biochimie

authors： Pinck M,Guilley E,Durr A,Hoff M,Pinck L,Fleck J

更新日期：1984-07-01 00:00:00

abstract：:The intervention of pyruvate in glucose metabolism was investigated during hypoxic stress in tumour cell cultures having respiratory capacities under normoxic conditions. Results obtained with nuclear magnetic resonance (NMR) spectroscopy showed that, under normoxic conditions, rat glioma C6 and human hepatoma Hep G2 ...

journal_title：Biochimie

authors： Perrin A,Roudier E,Duborjal H,Bachelet C,Riva-Lavieille C,Leverve X,Massarelli R

更新日期：2002-10-01 00:00:00

abstract：:The antiviral activity of L-nucleoside analogs depends in part on the enantioselectivity of nucleoside kinases which catalyse their monophosphorylation. The substrate properties of human recombinant deoxycytidine kinase (dCK) and human recombinant deoxyguanosine kinase (dGK) with respect to L-adenosine and L-guanosine...

journal_title：Biochimie

authors： Gaubert G,Gosselin G,Boudou V,Imbach JL,Eriksson S,Maury G

更新日期：1999-11-01 00:00:00

abstract：:Natural bovine seminal RNase possesses a potent antitumor action. We have mutagenized monomeric bovine pancreatic RNase A, devoid of any cytotoxic action, to insert residues present at corresponding positions in the subunit of dimeric, antitumor, seminal RNase. Like naturally dimeric seminal RNase, the mutant dimeric ...

journal_title：Biochimie

authors： Cafaro V,Bracale A,Formiggini F,Notomista E,D'Alessio G,Di Donato A

更新日期：1998-11-01 00:00:00

abstract：:A thrombin-like enzyme, named BjussuSP-I, isolated from Bothrops jararacussu snake venom, is an acidic single-chain glycoprotein with M(r)=61,000, pI approximately 3.8 and 6% sugar. BjussuSP-I shows high proteolytic activity upon synthetic substrates, such as S-2238 and S-2288. It also shows procoagulant and kallikrei...

journal_title：Biochimie

authors： Sant'Ana CD,Bernardes CP,Izidoro LF,Mazzi MV,Soares SG,Fuly AL,Zingali RB,Magro AJ,Braz AS,Fontes MR,Stábeli RG,Sampaio SV,Soares AM

更新日期：2008-03-01 00:00:00

abstract：:In recent years the chaperone HSC-70 has become a target for drug design with a strong focus in anticancer therapies. In our study of possible inhibitors of HSC-70 enzymatic activity we screened compounds by NMR as well as X-ray crystallography. As part of our screening efforts we crystallized the human HSC-70 ATP bin...

journal_title：Biochimie

authors： Zhang Z,Cellitti J,Teriete P,Pellecchia M,Stec B

更新日期：2015-01-01 00:00:00

abstract：:Snake venom from Agkistrodon acutus consists of a number of compounds which may potentially be used as drugs. However, it is hard to obtain enough pure protein for drug development. Recently, we reported expression and purification of a novel recombinant fibrinogenase which was named rFII. Here we reported for the fir...

journal_title：Biochimie

authors： Jiang W,Ma T,Su X,Qiu P,Yan G

更新日期：2009-02-01 00:00:00

abstract：:Isopentenyl adenosine derivatives can be found next to the anticodon (position 37) in tRNA from both the Bacteria and Eucarya domains. These modified nucleosides improve the efficiency of tRNA in translation, can increase and decrease translational fidelity, and make the tRNA less codon context sensitive. In bacteria ...

journal_title：Biochimie

authors： Persson BC,Esberg B,Olafsson O,Björk GR

更新日期：1994-01-01 00:00:00

abstract：:We have proposed an allosteric ATP inhibition mechanism of Pfk-2 determining the structure of different forms of the enzyme together with a kinetic enzyme analysis. Here we complement the mechanism by using hybrid oligomers of the homodimeric enzyme to get insights about the allosteric communication pathways between t...

journal_title：Biochimie

authors： Villalobos P,Soto F,Baez M,Babul J

更新日期：2016-09-01 00:00:00

abstract：:Hyper- (450 mOsm/l) and hypoosmotic exposure (150 mOsm/l) of Caco-2 cells, a human intestinal epithelial cell line, induced a twofold- and a fivefold increase in the production of IL-8, a constitutively expressed cytokine, respectively. This was observed both in the presence or in the absence of added proinflammatory ...

journal_title：Biochimie

authors： Hubert A,Cauliez B,Chedeville A,Husson A,Lavoinne A

更新日期：2004-08-01 00:00:00

abstract：:The lignan 8,8'-bis-(methylenedioxy)cinnamic acid (BMDCA) is a powerful competitive inhibitor (K1 = 2.0 microM) of the lignin peroxidase (LiP) from Phanerochaete chrysosporium and of the extracellular peroxidase of Phlebia radiata (I0.5 = 10 microM). BMDCA derivatives with the same double bond system also inhibited th...

journal_title：Biochimie

authors： Frías I,Trujillo JM,Romero J,Hernandez J,Pérez JA

更新日期：1995-01-01 00:00:00

abstract：:Dimethyl selenide (DMSe) and dimethyl diselenide (DMDSe) emissions by soil samples spiked with selenite or (methyl)selenocysteine, with or without a supplement of nutrient broth and glucose were measured. DMSe was the main form of volatile Se produced, and was observed for both Se-substrates. DMDSe was only emitted fr...

journal_title：Biochimie

authors： Favre-Bonté S,Ranjard L,Champier L,Cournoyer B,Nazaret S

更新日期：2006-11-01 00:00:00

abstract：:Ton-dependent colicins and microcins are actively taken up into sensitive cells at the expense of energy which is provided by the proton motive force of the cytoplasmic membrane. The Ton system consisting of the proteins TonB, ExbB and ExbD is required for colicin and microcin import. Colicins as well as the outer mem...

journal_title：Biochimie

authors： Braun V,Patzer SI,Hantke K

更新日期：2002-05-01 00:00:00

abstract：:The preadipocyte cell lines 3T3-L1 and 3T3-F442A are widely used to study the cellular mechanisms of preadipocyte differentiation and mature adipocyte functions. However, transfection with naked DNA is inefficient in these cell lines. Adenoviral gene transfer is a powerful technique to induce high levels of transgene ...

journal_title：Biochimie

authors： Béréziat V,Moritz S,Klonjkowski B,Decaudain A,Auclair M,Eloit M,Capeau J,Vigouroux C

更新日期：2005-11-01 00:00:00

abstract：:Oxidatively modified proteins are continuously produced in cells by reactive oxygen and nitrogen species generated as a consequence of aerobic metabolism. During periods of oxidative stress, protein oxidation is significantly increased and may become a threat to cell survival. In eucaryotic cells the proteasome has be...

journal_title：Biochimie

authors： Davies KJ

更新日期：2001-03-01 00:00:00

abstract：:Recombinant plasmids containing head-to-tail copies of different coat-protein replacement genomes of wheat dwarf virus (WDV) were used to study the mechanism leading to the release of replicating unit-length molecules in suspension culture cells of Triticum monococcum. For plasmids bearing two complete genomes, the vi...

journal_title：Biochimie

authors： Heyraud F,Matzeit V,Schaefer S,Schell J,Gronenborn B

更新日期：1993-01-01 00:00:00

abstract：:Epidemiological studies have demonstrated that the diabetes mellitus is a serious health burden for both governments and healthcare providers. The present study was hypothesized to evaluate the antihyperglycemic potential of fraxetin by determining the activities of key enzymes of carbohydrate metabolism in streptozot...

journal_title：Biochimie

authors： Murali R,Srinivasan S,Ashokkumar N

更新日期：2013-10-01 00:00:00

abstract：:Mouse brain myelin consists of a continuum of particles of different densities, as shown by sucrose density gradient centrifugation. In normal animals most of the material (65 per cent) is concentrated between 0.6 and 0.7 M sucrose (the maximum being found at 0.66 M sucrose, corresponding to 23 per cent). The density ...

journal_title：Biochimie

authors： Bourre JM,Pollet S,Daudu O,Le Saux F,Baumann N

更新日期：1977-01-01 00:00:00

abstract：:PA1b (Pea Albumin 1b) is a peptide toxin lethal for certain insects. This paper shows that the cultured insect cells Sf9 are sensitive to the toxin and display a high-affinity binding site for PA1b. Mammalian cells are not sensitive and no binding activity was detected. Signs of apoptosis of the Sf9 cells were observe...

journal_title：Biochimie

authors： Rahioui I,Laugier C,Balmand S,Da Silva P,Rahbe Y,Gressent F

更新日期：2007-12-01 00:00:00

abstract：:Using natural inducers of beta-glucuronidase, methyl-glucuronide and fructuronate, under gratuitous conditions (without metabolic conversion of these two compounds), we corroborate the fact that both molecules are required simultaneously in order to derepress the enzyme synthesis to a maximum level. Structurally relat...

journal_title：Biochimie

authors： Mandrand-Berthelot MA,Novel G,Novel M

更新日期：1977-01-01 00:00:00

abstract：:After 30 years of effort, the mammalian adenylate cyclase catalytic subunit has now been purified. It is a glycoprotein of 155 kDa, representing less than 0.01% of synaptosomal membrane protein. As measured in the presence of forskolin, its specific activity is 10-20 mumol of cAMP X mg-1 X min-1. The enzyme obtained i...

journal_title：Biochimie

authors： Monneron A,d'Alayer J,Coussen F

更新日期：1987-04-01 00:00:00

abstract：:High plasma choline has been associated with the metabolic syndrome and risk of chronic diseases, including cardiovascular disease. However, dietary choline is not correlated with choline plasma concentrations, and there are few studies and contradictory evidence regarding dietary choline and cardiovascular events. In...

journal_title：Biochimie

authors： Van Parys A,Lysne V,Svingen GFT,Ueland PM,Dhar I,Øyen J,Dierkes J,Nygård OK

更新日期：2020-06-01 00:00:00

abstract：:A human replication initiation protein, Cdt1, is a central player in the cell cycle regulation of DNA replication, and geminin down-regulates Cdt1 function by direct binding. It has been demonstrated that Cdt1 hyperfunction resulting from Cdt1-geminin imbalance, for example, by geminin silencing with small interfering...

journal_title：Biochimie

authors： Mizushina Y,Takeuchi T,Hada T,Maeda N,Sugawara F,Yoshida H,Fujita M

更新日期：2008-06-01 00:00:00

abstract：:The oxidation of beta-D-glucose with glucose oxidase generally requires oxygen, which, under normal conditions is present at low concentrations in the reaction medium. Experiments show that glucose oxidase is no longer saturated by oxygen at enzyme concentrations greater than 0.4 mg.ml1. This is due to the decrease in...

journal_title：Biochimie

authors： Boudrant J,Cheftel C

更新日期：1975-01-01 00:00:00

abstract：:Kinetic parameters characterizing the catalytic activities of enzymes are typically investigated in dilute solutions. However, in reality, these reactions occur in cells that, in addition to water and ions, are full of other macromolecules including proteins, nucleic acids, lipids, and metabolites. Such a crowded envi...

journal_title：Biochimie

authors： Popielec A,Ostrowska N,Wojciechowska M,Feig M,Trylska J

更新日期：2020-09-01 00:00:00

abstract：:The study of modified nucleoside contributions to RNA chemistry, structure and function has been thwarted by the lack of a site-selected method of incorporation which is both versatile and adaptable to present synthetic technologies. A reproducible and versatile site-selected incorporation of nine differently modified...

journal_title：Biochimie

authors： Agris PF,Malkiewicz A,Kraszewski A,Everett K,Nawrot B,Sochacka E,Jankowska J,Guenther R

更新日期：1995-01-01 00:00:00

abstract：:In the present study we evaluated the effect of chronic methionine administration on oxidative stress and biochemical parameters in liver and serum of rats, respectively. We also performed histological analysis in liver. Results showed that hypermethioninemia increased chemiluminescence, carbonyl content and glutathio...

journal_title：Biochimie

authors： Stefanello FM,Matté C,Pederzolli CD,Kolling J,Mescka CP,Lamers ML,de Assis AM,Perry ML,dos Santos MF,Dutra-Filho CS,Wyse AT

更新日期：2009-08-01 00:00:00

abstract：:The lipoprotein complex from human gallbladder bile was challenged with anti-IgA and anti-IgG antisera in order to determine whether the apolipoprotein complex isolated from the detergent-free form of bile lipoprotein contains IgA and IgG. The apolipoprotein complex indeed crossreacts with anti-IgA and anti-IgG. In ad...

journal_title：Biochimie

authors： Vigne JL,Nalbone G,Lafont H,Lairon D,Chabert C,Domingo N,Hauton JC

更新日期：1981-10-01 00:00:00

abstract：:Aptamers are short single-stranded nucleic acid molecules that are selected in vitro from a large random sequence library based on their high and specific affinity to a target molecule by a process known as SELEX. Cell-SELEX that employs whole living cells overexpressing the defined cell surface proteins (for selectio...

journal_title：Biochimie

authors： Takahashi M,Sakota E,Nakamura Y

更新日期：2016-12-01 00:00:00