Purification of the catalytic subunit of adenylate cyclase in vertebrates: state of the art in 1987.

abstract：:During the suckling period, the rats are fed continuously with milk, which is a high-fat low-carbohydrate diet (HF). At weaning, milk is progressively replaced by the rat's laboratory chow which is a high-carbohydrate low-fat diet (HCHO), and this is accompanied by large hormonal modifications: an increase in plasma i...

journal_title：Biochimie

authors： Girard J,Perdereau D,Narkewicz M,Coupé C,Ferré P,Decaux JF,Bossard P

更新日期：1991-01-01 00:00:00

abstract：:Previous reports on the carbohydrate specificities of Amaranthus caudatus lectin (ACL) and peanut agglutinin (PNA, Arachis hypogea) indicated that they share the same specificity for the Thomsen-Friedenreich (T(alpha), Galbeta1-3GalNAcalpha1-Ser/Thr) glycotope, but differ in monosaccharide binding--GalNAc>Gal (inactiv...

journal_title：Biochimie

authors： Wu AM,Wu JH,Yang Z,Singh T,Goldstein IJ,Sharon N

更新日期：2008-11-01 00:00:00

abstract：:The thyroid hormones L-thyroxine and triiodo-L-thyronine have profound effects on postembryonic development of most vertebrates. Analysis of their action in mammals is vitiated by the exposure of the developing foetus to a number of maternal factors which do not allow one to specifically define the role of thyroid hor...

journal_title：Biochimie

authors： Tata JR

更新日期：1999-04-01 00:00:00

abstract：:Insulin stimulates protein synthesis in skeletal muscle of young animals and has been reported to exert similar effects on a variety of mammalian cell types in culture. However, with chick embryo fibroblasts we found that the extent of this effect was very sensitive to the culture conditions of the cells prior to the ...

journal_title：Biochimie

authors： Jeffrey IW,Pain VM

更新日期：1993-01-01 00:00:00

abstract：:We have modified elongation factor Tu (EF-Tu) from Escherichia coli via mutagenesis of its encoding tufA gene to study its function-structure relationships. The isolation of the N-terminal half molecule of EF-Tu (G domain) has facilitated the analysis of the basic EF-Tu activities, since the G domain binds the substra...

journal_title：Biochimie

authors： Anborgh PH,Cool RH,Gümüsel F,Harmark K,Jacquet E,Weijland A,Mistou MY,Parmeggiani A

更新日期：1991-07-01 00:00:00

abstract：:The targets of in vivo studies of the ribosomal assembly process are mainly the events of rRNA processing, whereas in vitro studies (total reconstitution) focus on principles of the assembly process such as assembly-initiation proteins, rate-limiting steps and a detailed sequence of assembly reactions (assembly map). ...

journal_title：Biochimie

authors： Nierhaus KH

更新日期：1991-06-01 00:00:00

abstract：:Clustered apurinic/apyrimidinic (AP) sites are more cytotoxic than isolated AP lesions because double strand breaks (DSB) can be formed during repair of closely positioned bistranded AP sites. Formation of DSB due to simultaneous cleavage of bistranded AP sites may be regulated by proteins specifically interacting wit...

journal_title：Biochimie

authors： Ilina ES,Khodyreva SN,Lavrik OI

更新日期：2018-07-01 00:00:00

abstract：:Thyroglobulin is a large-size iodoglycoprotein specific to thyroid tissue and is the substrate for the synthesis of thyroid hormones, thyroxine and 3,5,3'-triiodothyronine. Recent studies, which greatly benefited from recombinant DNA methodologies, improved the knowledge of several structural features of this dimeric ...

journal_title：Biochimie

authors： Malthièry Y,Marriq C,Bergé-Lefranc JL,Franc JL,Henry M,Lejeune PJ,Ruf J,Lissitzky S

更新日期：1989-02-01 00:00:00

abstract：:Tryptocidine C (TpcC, cyclo[D-Phe1-Pro2-Trp3-D-Trp4-Asn5-Gln6-Trp7-Val8-Orn9-Leu10]) is a broad-spectrum antimicrobial peptide in the tyrothricin complex produced by a soil bacterium, Brevibacillus parabrevis. Electrospray mass spectrometric studies reveal the oligomerisation of TpcC into dimers and higher oligomers, ...

journal_title：Biochimie

authors： Rautenbach M,Kumar V,Vosloo JA,Masoudi Y,van Wyk RJ,Stander MA

更新日期：2020-12-18 00:00:00

abstract：:Cell-free extracts of fungal mycelia of two aflatoxin non-producing isolates of Aspergillus parasiticus (SRRC 163 and SRRC 2043) were utilized for the study of enzyme activities involved in the latter stages of aflatoxin biosynthesis. The post-microsomal fractions (105,000 x g supernatant) of both SRRC 163 and SRRC 20...

journal_title：Biochimie

authors： Bhatnagar D,Cleveland TE

更新日期：1988-06-01 00:00:00

abstract：:Some physico-chemical properties of R140G and K141Q mutants of human small heat shock protein HspB1 associated with hereditary peripheral neuropathy were analyzed. Mutation K141Q did not affect intrinsic Trp fluorescence and interaction with hydrophobic probe bis-ANS, whereas mutation R140G decreased both intrinsic fl...

journal_title：Biochimie

authors： Nefedova VV,Datskevich PN,Sudnitsyna MV,Strelkov SV,Gusev NB

更新日期：2013-08-01 00:00:00

abstract：:The recF gene of E coli lies within a cluster of genes which play essential roles in DNA replication; the gene order is dnaA dnaN recF gyrB. Each of these genes has its own promoters which, with the exception of dnaA promoters, reside entirely within the translated region of the respective preceding gene. In this repo...

journal_title：Biochimie

authors： Pérez-Roger I,García-Sogo M,Navarro-Aviñó JP,López-Acedo C,Macián F,Armengod ME

更新日期：1991-02-01 00:00:00

abstract：:The product of the sex-determining gene SRY is a member of the HMG box containing protein superfamily. The HMG box is a DNA-binding domain of about 80 amino acids shared by many proteins with diverse functions. It seems that the functions of the full length protein are restricted to the HMG box but their molecular bas...

journal_title：Biochimie

authors： Soullier S,Poulat F,Boizet-Bonhoure B,Calas B,Bennes R,Heitz F,Berta P

更新日期：1994-01-01 00:00:00

abstract：:The Escherichia coli low molecular mass penicillin-binding proteins (PBP4, PBP5 and PBP6) are a group of penicillin-sensitive enzymes involved in the final stages of cell wall assembly. It has been suggested that these proteins may interact with the periplasmic face of the inner membrane via C-terminal amphiphilic alp...

journal_title：Biochimie

authors： Harris F,Phoenix DA

更新日期：1997-04-01 00:00:00

abstract：:Using a new extended set of 13 amber suppressors in E coli, systematic amino-acid replacements were performed at positions 104(E) and 238(G) of TEM-1 beta-lactamase from PUC19. The enzyme is tolerant to most substitutions tested at position 104. Missense revertants E104K, E104S or E104Y exhibited only minor changes in...

journal_title：Biochimie

authors： Lenfant F,Labia R,Masson JM

更新日期：1990-06-01 00:00:00

abstract：:A number of specific Fab and Fv fragments and their complexes with antigens (avian lysozymes), haptens, and anti-idiotopic Fabs have been studied by immunochemical and crystallographic techniques. Antigen and antibody interact through closely complementary contacting surfaces, without major conformational changes. An ...

journal_title：Biochimie

authors： Tello D,Spinelli S,Souchon H,Saul FA,Riottot MM,Mariuzza RA,Lascombe MB,Houdusse A,Eiselé JL,Fischmann T

更新日期：1990-08-01 00:00:00

abstract：:Epithelial Ca(2+)-regulation, which governs cornified envelope formation in the skin epidermis and hair follicles, closely coincides with the expression of S100A3, filaggrin and trichohyalin, and the post-translational modification of these proteins by Ca(2+)-dependent peptidylarginine deiminases. This review summariz...

journal_title：Biochimie

authors： Kizawa K,Takahara H,Unno M,Heizmann CW

更新日期：2011-12-01 00:00:00

abstract：:Pulmonary fibrosis (PF) is a progressive lethal disorder. In this study, the effect of daidzein, a soyisoflavone against Bleomycin (BLM) induced PF in rats was elucidated. A single intratracheal instillation of BLM (3 U/kg.bw) was administered in rats to induce PF. Daidzein (0.2 mg/kg) was administered subcutaneously,...

journal_title：Biochimie

authors： Soumyakrishnan S,Divya T,Kalayarasan S,Sriram N,Sudhandiran G

更新日期：2014-08-01 00:00:00

abstract：:An immunological demonstration of alpha-fetoprotein (AFP) in uterine cytosol from immature rats is reported. The identity between serum AFP and the estrogen binding 4.5 S macromolecular complex of uterine cytosol was demonstrated by the use of a specific immunoadsorbent to AFP. Analysis of the sedimentation profile ...

journal_title：Biochimie

authors： Aussel C,Uriel J,Michel G,Baulieu EE

更新日期：1974-01-01 00:00:00

abstract：:The four known ID proteins (ID1-4, Inhibitor of Differentiation) share a homologous helix loop helix (HLH) domain and act as dominant negative regulators of basic-HLH transcription factors. ID proteins also interact with many non-bHLH proteins in complex networks. The expression of ID proteins is increasingly observed...

journal_title：Biochimie

authors： Sharma P,Chinaranagari S,Chaudhary J

更新日期：2015-05-01 00:00:00

abstract：:rBmTI-A is a recombinant serine protease inhibitor that belongs to the Kunitz-BPTI family and that was cloned from Rhipicephalus microplus tick. rBmTI-A has inhibitory activities on bovine trypsin, human plasma kallikrein, human neutrophil elastase and plasmin with dissociation constants in nM range. It is characteriz...

journal_title：Biochimie

authors： Bomediano Camillo LM,Ferreira GC,Duran AFA,da Silva FRS,Garcia W,Scott AL,Sasaki SD

更新日期：2020-12-24 00:00:00

abstract：:Esterases and lipases both hydrolyse ester bonds. Whereas the lipases display high activity towards the aggregated state of its substrate, the esterases typically show highest activity towards the soluble state of its substrate. We have compared the amino acid sequence, the 3D-structure as well as the pH-dependent ele...

journal_title：Biochimie

authors： Fojan P,Jonson PH,Petersen MT,Petersen SB

更新日期：2000-11-01 00:00:00

abstract：:At least 6 N-acetylglucosaminyltransferases (GlcNAc-T I, II, III, IV, V and VI) are involved in initiating the synthesis of the various branches found in complex asparagine-linked oligosaccharides (N-glycans), as indicated below: GlcNAc beta 1-6 GlcNAc-T V GlcNAc beta 1-4 GlcNAc-T VI GlcNAc beta 1-2Man alpha 1-6 GlcNA...

journal_title：Biochimie

authors： Brockhausen I,Narasimhan S,Schachter H

更新日期：1988-11-01 00:00:00

abstract：:The development of in vitro repair assays with human cell-free extracts led to new insights on the mechanism of excision of DNA damage which consists of incision/excision and repair synthesis/ligation. We have adapted the repair synthesis reaction with cells extracts incubated with damaged plasmid DNA performed in liq...

journal_title：Biochimie

authors： Salles B,Rodrigo G,Li RY,Calsou P

更新日期：1999-01-01 00:00:00

abstract：:Neuropeptides and hormones, in contrast to constitutive secretory proteins, are sorted to and stored in secretory granules and released upon a stimulus. During the last two decades, signals and mechanisms involved in their sorting to the regulated pathway of protein secretion have been addressed in numerous studies. T...

journal_title：Biochimie

authors： Glombik MM,Gerdes HH

更新日期：2000-04-01 00:00:00

abstract：:DNA mismatch repair (MMR) is an important replication error avoidance mechanism that prevents mutation. The association of defective MMR with familial and sporadic gastrointestinal and endometrial cancer has been acknowledged for some years. More recently, it has become apparent that MMR defects are common in acute my...

journal_title：Biochimie

authors： Karran P,Offman J,Bignami M

更新日期：2003-11-01 00:00:00

abstract：:The NADPH-oxidase complex, which plays beneficial or detrimental role in the inflammatory and degenerative diseases, is a membrane multi-subunit complex tightly regulated in order to produce superoxide anions, precursor of oxygen reactive species (ROS), in cells. The flavocytochrome b(558) (Cytb(558)) is the catalytic...

journal_title：Biochimie

authors： Souabni H,Machillot P,Baciou L

更新日期：2014-12-01 00:00:00

abstract：:Halophilic proteins have evolved to be soluble, stable and active in high salt concentration. Crystallographic studies have shown that surface enrichment by acidic amino acids is a common structural feature of halophilic proteins. In addition, ion-binding sites have also been observed in most of the cases. The role of...

journal_title：Biochimie

authors： Madern D,Ebel C

更新日期：2007-08-01 00:00:00

abstract：:The Nicotiana tabacum Nt-4/1 protein is a plant-specific protein of unknown function. Analysis of bacterially expressed Nt-4/1 protein in vitro revealed that the protein secondary structure is mostly alpha-helical and suggested that it could consist of three structural domains. Earlier studies of At-4/1, the Arabidops...

journal_title：Biochimie

authors： Solovyev AG,Minina EA,Makarova SS,Erokhina TN,Makarov VV,Kaplan IB,Kopertekh L,Schiemann J,Richert-Pöggeler KR,Morozov SY

更新日期：2013-07-01 00:00:00

abstract：:The binding of Ca2+ to membrane models composed of diplamitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylethanolamine (DPPE) 15N-labeled in the polar head group was investigated at pH 8.5 and pH 9.4 by 15N-NMR spectroscopy. Both phospholipids exhibit a decrease in the chemical shift anisotropy, indicating c...

journal_title：Biochimie

authors： Akoka S,Tellier C

更新日期：1989-01-01 00:00:00