Abstract:
:Esterases and lipases both hydrolyse ester bonds. Whereas the lipases display high activity towards the aggregated state of its substrate, the esterases typically show highest activity towards the soluble state of its substrate. We have compared the amino acid sequence, the 3D-structure as well as the pH-dependent electrostatic signature of selected members of the two families, for which 3D-structural information is publicly available. Lipases display a statistically significant enhanced occurrence of non-polar residues close to the surface, clustering around the active-site. Lid opening appears to strengthen this pattern further. As we have proposed earlier the active site of lipases displays negative potential in the pH-range associated with their maximum activity, typically at pH values above 8. The esterases show a very similar pattern, however, at pH values around 6 correlated with their usually lower pH-activity optimum.
journal_name
Biochimiejournal_title
Biochimieauthors
Fojan P,Jonson PH,Petersen MT,Petersen SBdoi
10.1016/s0300-9084(00)01188-3keywords:
subject
Has Abstractpub_date
2000-11-01 00:00:00pages
1033-41issue
11eissn
0300-9084issn
1638-6183pii
S0300-9084(00)01188-3journal_volume
82pub_type
杂志文章,评审相关文献
BIOCHIMIE文献大全abstract::Unlike most tonoplast ATPases, the vacuolar ATPase of Acer pseudoplatanus cells (Km = 0.4 mM) was strongly inhibited by vanadate (I50 = 10 microM). The inhibition was non-competitive. Chemicals usually added in the reaction mixture either increase (NH+4, K+) or decrease (Na+, EDTA) the ATPase inhibition. However, thes...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(89)90044-8
更新日期:1989-07-01 00:00:00
abstract::This paper deals with the discovery of plasmalogen deficiency in the cerebro-hepato-renal (Zellweger) syndrome and discusses how this has led to the development of postnatal and prenatal diagnostic procedures for this and a number of related peroxisomal disorders in man that show a general impairment in the biosynthes...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(93)90076-5
更新日期:1993-01-01 00:00:00
abstract::Hemorrhage is the most potent manifestation of envenomation by Vipera ammodytes ammodytes (V. a. ammodytes) venom in man. A detailed description of the venom components contributing to this effect is thus medically very important. We have characterized a novel component, termed here VaH3, as a potently hemorrhagic sna...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.01.003
更新日期:2013-06-01 00:00:00
abstract::Oxidative stress is the main mechanism behind efficient disinfectants, causing damage in bacterial macromolecules. Importantly, bacteria activate resistance mechanisms in response to damage generated by oxidative stress. Strategies allowing pathogens to survive oxidative stress are highly conserved among microorganism...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2020.11.009
更新日期:2021-01-01 00:00:00
abstract::The oncogenic potential of the high-risk human papillomavirus (HPV) relies on the expression of genes specifying the E7 and E6 proteins. To investigate further the variation in oligomeric structure that has been reported for different E7 proteins, an HPV-18 E7 cloned from a Hispanic woman with cervical intraepithelial...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.02.028
更新日期:2010-07-01 00:00:00
abstract::Pseudouridine [Ψ] is a frequent base modification in the ribosomal RNA [rRNA] and may be involved in the modulation of the conformational flexibility of rRNA helix-loop structures during protein synthesis. Helix 69 of 23S rRNA contains pseudouridines at the positions 1911, 1915 and 1917 which are formed by the helix 6...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.12.018
更新日期:2011-05-01 00:00:00
abstract::Porphyromonas gingivalis uses a type IX secretion system (T9SS) to deliver more than 30 proteins to the bacterial surface using a conserved C-terminal domain (CTD) as an outer membrane translocation signal. On the surface, the CTD is cleaved and an anionic lipopolysaccharide (A-PLS) is attached by PorU sortase. Among ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2019.06.010
更新日期:2019-11-01 00:00:00
abstract::The thrombin-binding DNA aptamer (TBA) 5'-d(GGTTGGTGTGGTTGG)-3' forms a G-quadruplex that is necessary for binding to the coagulation factor thrombin. The stability of the G-quadruplex of TBA when bound to thrombin and potassium ion (K(+)) were investigated for the wild-type oligonucleotide and for mutants in which th...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2011.03.013
更新日期:2011-08-01 00:00:00
abstract::The effects of various concentrations of 2-fluoro-2-deoxy-D-glucose (FDG) on the aerobic metabolism of glucose and the reciprocal effect of glucose on the metabolism of FDG in glucose-grown repressed Saccharomyces cerevisiae cells were studied at 30 degrees C in a standard pyrophosphate medium containing 5 x 10(7) cel...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(96)88129-6
更新日期:1995-01-01 00:00:00
abstract::The solubilizing powers of taurocholate, taurochenodeoxycholate and tauroursodeoxycholate for monoolein and cholesterol, and the size of the bile salt-monoolein-cholesterol micelles have been determined. For the three bile salt species, the micellar size depends on the saturation with monoolein. As a result, for a giv...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(82)80492-6
更新日期:1982-04-01 00:00:00
abstract::Previous work from this laboratory has shown that T7 RNA polymerase outpaces ribosomes in vivo, generating naked mRNA stretches which may be nuclease-sensitive. In particular, lacZ transcripts synthesised this way are highly unstable and yield little beta-galactosidase. We have argued that most of these transcripts ar...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(96)84747-x
更新日期:1996-01-01 00:00:00
abstract::The polysaccharide from Aloe vahombe (lilaceae) was submitted to partial hydrolyses by sulfuric, oxalic and phosphoric acid. Some oligosaccharides were isolated and investigated by chemical and 13C NMR spectroscopic methods. Their structure was determined. The results prove unambiguously that in the analyzed material ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(84)90151-2
更新日期:1984-07-01 00:00:00
abstract::Bacillus subtilis strain SO113 secretes a pectate lyase which is produced during the exponential death phase of growth, just before sporulation. This extracellular pectate lyase, which produces unsaturated products from polygalacturonate, was purified 35-fold from the culture supernatant of Bacillus subtilis by a CM S...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(90)90053-j
更新日期:1990-09-01 00:00:00
abstract::The proton NMR data of a synthetic model of deoxy-myoglobin and hemoglobin show one that:(i) the ground state of the high spin iron (II) complex must be described by at least two electronic levels separated by ca.200 cm-1 and resulting from the removal of the degeneracy of the 5E level; (ii) this in-plane rhombic dist...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(82)80285-x
更新日期:1981-11-01 00:00:00
abstract::Estradiol-PAB-cellulose, an easily prepared adsorbent, has been proposed to purify the uterine estradiol receptor according to the principle of biospecific affinity chromatography. It apparently removes all hormone binding sites when cytosol preparations are incubated with it. A systematic study of this adsorbent was ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(76)80317-3
更新日期:1976-01-01 00:00:00
abstract::In the present study we evaluated the effect of chronic methionine administration on oxidative stress and biochemical parameters in liver and serum of rats, respectively. We also performed histological analysis in liver. Results showed that hypermethioninemia increased chemiluminescence, carbonyl content and glutathio...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2009.04.018
更新日期:2009-08-01 00:00:00
abstract::The nucleotide sequence of the gene encoding chicken ribosomal protein L7a was determined. The gene contains eight exons and seven introns, which spread over 3613 nucleotides. The transcription initiation sites were located on four consecutive nucleotides GCCC, which are at the 5' terminus of a short polypyrimidine tr...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(93)90128-f
更新日期:1993-01-01 00:00:00
abstract::Neprilysin is also known as skin fibroblast-derived elastase, and its up-regulation during aging is associated with impairments of the elastic fiber network, loss of skin elasticity and wrinkle formation. However, information on its elastase activity is still limited. The aim of this study was to investigate the degra...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2017.11.018
更新日期:2018-03-01 00:00:00
abstract::In the bacterium Escherichia coli, H-NS-(H1, H1a) is a heat-stable protein with a molecular mass of 15.5 kDa involved in nucleoid organisation and gene regulation linked to certain signal transduction pathways. We have shown that, following addition of preparations of everted inner membrane vesicles, heat-stable cleav...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(97)80025-9
更新日期:1997-06-01 00:00:00
abstract::Thyroid peroxidase is a member of a family of mammalian peroxidases that includes myeloperoxidase, lactoperoxidase, eosinophil peroxidase, and salivary peroxidase. Protein sequences showing a high degree of sequence similarity with mammalian peroxidases have recently been observed in several invertebrate species. A mu...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(99)80110-2
更新日期:1999-05-01 00:00:00
abstract::MicroRNAs (miRNAs) play profound roles in plant growth and development by regulating gene expression. Tetraploid plants often have better physical characteristics and stress tolerance than their diploid progenitors, but the role of miRNAs in this superiority is unclear. Paulownia tomentosa, (Paulowniaceae) is attracti...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.02.008
更新日期:2014-07-01 00:00:00
abstract::P53 is a transcription factor also called the "cellular gatekeeper of genome" because it can induce cell cycle arrest in G1, apoptosis or affect DNA replication in response to DNA damage. Wild type p53 is localised in both the cytoplasm and nucleus of first trimester trophoblastic cells (CTB). Immunoblotting of CTB wi...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2008.02.006
更新日期:2008-06-01 00:00:00
abstract::Human α(1)-acid glycoprotein (AGP) is a positive acute phase plasma protein containing two disulfide bridges. Structural studies have shown that under specific conditions AGP undergoes aggregation. In this study, we analysed the nature of AGP's aggregates formed under reducing and non-reducing conditions at pH 5.5 and...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.09.005
更新日期:2013-02-01 00:00:00
abstract::Crystals of flavoenzyme L-lactate oxidase from Aerococcus viridans (LOX) have been obtained that diffract to 3.0 A resolution (P2(1)2(1)2(1), a = 118.4 A, b = 138.4 A, c = 194.6 A). Crystallographic studies suggest that the enzyme may exist as an octameric form with non-crystallographic two- and four-fold axes in the ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(98)80072-2
更新日期:1998-04-01 00:00:00
abstract::The innate immunity factor lactoferrin harbours two antimicrobial moieties, lactoferricin and lactoferrampin, situated in close proximity in the N1 domain of the molecule. Most likely they cooperate in many of the beneficial activities of lactoferrin. To investigate whether chimerization of both peptides forms a funct...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2008.05.019
更新日期:2009-01-01 00:00:00
abstract::The x-ray structure of the monotopic membrane protein (S)-mandelate dehydrogenase (MDH) from Pseudomonas putida reveals an inherent flexibility of its membrane binding segment that might be important for its biological activity. The surface of MDH exhibits a concentration of the positive charges on one side and the ne...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2018.07.017
更新日期:2018-11-01 00:00:00
abstract::Diacylglycerol kinase (DGK) is a lipid-metabolizing enzyme that phosphorylates diacylglycerol (DG) to produce phosphatidic acid (PA). DGKδ is highly expressed in the skeletal muscle, and a decrease in DGKδ expression increases the severity of type 2 diabetes. However, the role of DGKδ in myogenic differentiation is st...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2018.05.017
更新日期:2018-08-01 00:00:00
abstract::The adaptive response appears in E. coli after exposure to low levels of alkylating agents. This system is under the positive control of the ada gene. At least two enzymes are induced during the response: 3-methyladenine DNA glycosylase II and O6-methylguanine DNA methyltransferase. The latter is also the product of t...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(85)80080-8
更新日期:1985-03-01 00:00:00
abstract::Mouse brain myelin consists of a continuum of particles of different densities, as shown by sucrose density gradient centrifugation. In normal animals most of the material (65 per cent) is concentrated between 0.6 and 0.7 M sucrose (the maximum being found at 0.66 M sucrose, corresponding to 23 per cent). The density ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(77)80213-7
更新日期:1977-01-01 00:00:00
abstract::Murine L5178Y cell ribosomes were dissociated into subunits either with potassium chloride in the presence of puromycin or with the chelating agent EDTA. The proteins of ribosomal subunits obtained by these different methods were compared by means of bidimensional polyacrylamide gel electrophoresis. KCl-derived 60S an...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(80)80225-2
更新日期:1979-01-01 00:00:00