Abstract：
:The x-ray structure of the monotopic membrane protein (S)-mandelate dehydrogenase (MDH) from Pseudomonas putida reveals an inherent flexibility of its membrane binding segment that might be important for its biological activity. The surface of MDH exhibits a concentration of the positive charges on one side and the negative charges on the other side. The putative membrane binding surface of MDH has a concentric circular ridge, formed by positively charged residues, which projects away from the protein surface by ∼4 Å; this is an unique structural feature and not observed in other monotopic membrane proteins to our knowledge. There are three α-helixes in the membrane binding region. Based on the structure of MDH, it is possible to propose that the interaction of MDH with the membrane is stabilized by coplanar electrostatic interactions, between the positively charged concentric circular ridge and the negatively charged head-groups of the phospholipid bilayer, along with three α-helixes that provide additional stability by inserting into the membrane. The structure reveals the possible orientation of these helixes along with possible roles for the individual residues which form those helixes. These α-helixes may play a role in the enzyme's mobility. A detergent molecule, N-Dodecyl-β-maltoside, is inserted between the membrane binding region and rest of the molecule and may provide structural stability to intra-protein regions by forming hydrogen bonds and close contacts. From the average B-factor of the MDH structure, it is likely that MDH is highly mobile, which might be essential for its interaction in membrane and non-membrane environments, as its substrate (S)-mandelate, is from the cytoplasm, while its electron acceptor is a component of the membrane electron transport chain.
journal_name
Biochimiejournal_title
Biochimieauthors
Sukumar N,Liu S,Li W,Mathews FS,Mitra B,Kandavelu Pdoi
10.1016/j.biochi.2018.07.017subject
Has Abstractpub_date
2018-11-01 00:00:00pages
45-54eissn
0300-9084issn
1638-6183pii
S0300-9084(18)30212-8journal_volume
154pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:Contradictory results for concentrations of vitamin B12, holotranscobalamin (holoTC), and methylmalonic acid (MMA) have been reported. We tested the hypothesis that the extracellular vitamin B12 markers are not reflecting the intracellular vitamin B12-dependent biochemical reactions in individuals with type 2 diabetes...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2012.10.028
更新日期：2013-05-01 00:00:00
abstract：:In recent years the chaperone HSC-70 has become a target for drug design with a strong focus in anticancer therapies. In our study of possible inhibitors of HSC-70 enzymatic activity we screened compounds by NMR as well as X-ray crystallography. As part of our screening efforts we crystallized the human HSC-70 ATP bin...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2014.11.012
更新日期：2015-01-01 00:00:00
abstract：:Although up to 25% of glucose released into circulation in the postabsorptive state comes from renal gluconeogenesis, the regulatory mechanisms of this process are still poorly recognized, comparing to hepatic ones. The aim of the present study was to examine if hypoxia-inducible factor-1 (HIF-1) might be involved in ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.02.002
更新日期：2020-01-01 00:00:00
abstract：:Haloalkane dehalogenases are known as bacterial enzymes cleaving a carbon-halogen bond in halogenated compounds. Here we report the first biochemically characterized non-microbial haloalkane dehalogenase DspA from Strongylocentrotus purpuratus. The enzyme shows a preference for terminally brominated hydrocarbons and e...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.07.025
更新日期：2013-11-01 00:00:00
abstract：:In order to study the relationship between bile cholesterol and free cholesterol carried by high and low density lipoproteins (HDL and LDL), 10 male Wistar rats, 11 weeks old and fed with a standard diet were divided into 3 groups which received an intravenous infusion (jugular vein) of either LDL, HDL or liposomes. L...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(87)90270-7
更新日期：1987-01-01 00:00:00
abstract：:We identified a T-to-C mutation 2 nucleotides (nt) upstream from the AG in a GT-AG intron between exons 2 and 3 in the human T-cell leukemia virus type I (HTLV-I) tax mRNA. This mutation resulted in the preferential usage of an alternative splice site, causing a 75-nt elongation of tax mRNA and reduced production of v...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2005.07.013
更新日期：2006-02-01 00:00:00
abstract：:New strains of basidiomycetes producing extracellular laccases (Trametes ochracea 92-78, and Trametes hirsuta 56) have been found by screening of isolates of Trametes fungi. The laccases from T. hirsuta 56 and T. ochracea 92-78 as well as two laccases from previously found and described strains of basidiomycetes, name...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2004.08.005
更新日期：2004-09-01 00:00:00
abstract：:The interactions of 6-8 ring hairpin polyamides (PAs) with the minor groove of DNA have been investigated extensively. More recent studies of large antiviral PAs (14-20 rings) active against small DNA tumor viruses lead to questions regarding the extent to which the DNA binding behaviors of the well studied, smaller P...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2019.07.021
更新日期：2019-10-01 00:00:00
abstract：:Over the last decades, mesenchymal stem cells (MSCs) have been extensively studied with regard to their potential applications in regenerative medicine. In rheumatic diseases, MSC-based therapy is the subject of great expectations for patients who are refractory to proposed treatments such as rheumatoid arthritis (RA)...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2013.04.017
更新日期：2013-12-01 00:00:00
abstract：:Recently we have found that cytokine IL-2 and innate immunity protein Tag7 activate cytotoxic lymphocytes that kill HLA-negative tumor cells, inducing both apoptosis and necroptosis. Here we decrypt the processes, taking part in necroptosis execution after FasL-Fas interaction. Necroptosis begins with RIPK1 activation...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2018.07.003
更新日期：2018-09-01 00:00:00
abstract：:After the assessment of the antitumor activity of the anthracycline pigments, the S peucetius group of metabolites was discovered and eventually doxorubicin, a major anticancer agent of established clinical usefulness was developed in the early seventies. A second generation of compounds followed, represented mainly b...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(98)80003-5
更新日期：1998-03-01 00:00:00
abstract：:A variety of transcription attenuation mechanisms are used by bacteria to regulate gene and operon expression. This review summarizes previous and current studies designed to elucidate the features of the specific attenuation mechanisms that regulate expression of the tryptophanase (tna) operon of Escherichia coli and...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(97)86725-9
更新日期：1996-01-01 00:00:00
abstract：:Leptin is a cytokine secreted by the adipose tissue that is involved in the control of body weight. We previously showed that a point mutation (R105W) in leptin results in leptin deficiency, marked obesity and hypogonadism in humans adults. Expression in COS1 cells showed impaired secretion and intracellular accumulat...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2004.06.005
更新日期：2004-06-01 00:00:00
abstract：:Three mutanase (alpha-1,3-glucanase)-producing microorganisms isolated from soil samples were identified as a relatives of Paenibacillus. A mutanase was purified to homogeneity from cultures of each, and the molecular masses of the purified enzymes were approximately 132, 141, and 141kDa, respectively. The correspondi...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2007.09.018
更新日期：2008-03-01 00:00:00
abstract：:The hydrolysis of trioctanoin and of monoolein by rat intestinal homogenates, catalyzed by the previously described "trioctanoin hydrolase" and "monoglyceride lipase" [Ref. 6 and 11], was shown to be entirely due to the action of the intestinal glycerol-ester hydrolase [Ref. 1 and 2] and to contaminating pancreatic li...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(77)80320-9
更新日期：1977-01-01 00:00:00
abstract：:Most evolutionists agree to consider that our present RNA/DNA/protein world has originated from a simpler world in which RNA played both the role of catalyst and genetic material. Recent findings from structural studies and comparative genomics now allow to get a clearer picture of this transition. These data suggest ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2005.03.015
更新日期：2005-09-01 00:00:00
abstract：:Some oxysterols resulting either from enzymatic oxidation or autoxidation of cholesterol are associated with age-related diseases including neurodegenerative diseases. Among these oxysterols, 7β-hydroxycholesterol (7β-OHC) is often found at increased levels in patients. It is therefore important to identify molecules ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2018.06.027
更新日期：2018-10-01 00:00:00
abstract：:The biosynthesis of the beta-lactam antibiotic penicillin is an excellent model for the study of secondary metabolites produced by filamentous fungi due to the good background knowledge on the biochemistry and molecular genetics of the beta-lactam producing microorganisms. The three genes (pcbAB, pcbC, penDE) encoding...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2008.09.004
更新日期：2009-02-01 00:00:00
abstract：:At the first symptoms of organisation of the silkgland in the embryo, mitoses stop and nuclei start to grow. Through autoradiographic studies, performed after sequenced labeling with [3H] and [14C] thymidine, the durations of the different phases of DNA synthesis cycles (T = 42 to 48 h, S = 22 to 25 h, G = 20 to 23 h)...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(79)80066-8
更新日期：1979-01-01 00:00:00
abstract：:Viral expression was analyzed in ten cell clones of a Friend erythroleukemia cell line (HFL/b cell line [3]), which had lost its capacity to produce infectious particles. All the ten subclones were non producers but expressed spleen focus forming virus (SFFV) polypeptides in the form of p48-p50gag and gp50-gp52env. On...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(83)80277-6
更新日期：1983-04-01 00:00:00
abstract：:The distribution of fatty acids among cellular glycerophospholipids is finely regulated by the CoA-dependent acylation of lysophospholipids followed by transacylation reactions. Arachidonic acid is the fatty acid precursor of a wide family of bioactive compounds called the eicosanoids, with key roles in innate immunit...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2009.11.010
更新日期：2010-06-01 00:00:00
abstract：:To examine the role of the C-terminal domain in the chaperone function of trigger factor (TF), a number of truncation mutants were constructed, namely: TF419, TF389, TF380, TF360, TF344, and TF251, in which the C-terminal 13, 43, 52, 72, 88 residues or the entire C-domain were deleted, respectively. Co-expression of m...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2005.11.006
更新日期：2006-06-01 00:00:00
abstract：:Unspliced human immunodeficiency virus-1 (HIV-1) mRNA is capped and therefore can be translated via conventional scanning mechanism. In addition, its 5' untranslated region (5'UTR) is thought to function as an internal ribosome entry site (IRES) during G2/M-phase of cell cycle or when cap-dependent translation is inhi...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2015.12.004
更新日期：2016-02-01 00:00:00
abstract：:In order to identify conserved potential secondary structures within translational start sites, mRNA sequences derived from different species were studied with programs able to depict such features. The potential secondary structure of 71 bases around the initiator AUG or AUGs in the coding sequences of 290 eukaryotic...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(94)90120-1
更新日期：1994-01-01 00:00:00
abstract：:The effect of cycloheximide on rRNA and poly (A) (+) RNA synthesis is studied in radish seedlings. When used at a concentration of 50 microng/ml cycloheximide selectively blocks rRNA synthesis without altering poly (A) (+) RNA synthesis. Processing of the 2.3 X 10(6) dalton pre-rRNA is severely impaired. This observat...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(77)80085-0
更新日期：1977-01-01 00:00:00
abstract：:Stroke is a worldwide major cause of mortality and morbidity. Preclinical studies have identified over 1000 molecules with brain-protective properties. More than 200 clinical trials have evaluated neuroprotective candidates for ischemic stroke yet, to date almost all failed, leading to a re-analysis of treatment strat...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2015.06.005
更新日期：2016-01-01 00:00:00
abstract：:Numerous studies have reported the beneficial effects of antioxidants in human diseases. Among their biological effects, a majority of antioxidants scavenge reactive radicals in the body, thereby reducing oxidative stress that is associated with the pathogenesis of many diseases. Antioxidant dendrimers are a new class...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2015.02.001
更新日期：2015-04-01 00:00:00
abstract：:We present an approach for designing a knowledge-based system, called Sequence Acquisition In Context (SAIC), that will be able to cooperate with a biologist in the analysis of DNA sequences. The main task of the system is the acquisition of the expert knowledge that the biologist uses for solving ambiguities from gel...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(93)90167-q
更新日期：1993-01-01 00:00:00
abstract：:Poly(ADP-ribosyl)ation is a posttranslational protein modification significant for genomic stability and cell survival in response to DNA damage. Poly(ADP-ribosyl)ation is catalyzed by poly(ADP-ribose)polymerases (PARPs). Among the 17 members of the PARP family, PARP-1 and PARP-2 are described as enzymes whose catalyt...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.01.007
更新日期：2013-06-01 00:00:00
abstract：:Excitation-contraction coupling describes the series of events that beginning with propagated action potential on the muscle fiber surface membrane, leads to the twitch contraction of the fiber. Myotonic disorders are inherited diseases whose clinical manifestations include electrophysiological signs such as increased...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(87)90032-0
更新日期：1987-04-01 00:00:00
