Abstract:
:Three mutanase (alpha-1,3-glucanase)-producing microorganisms isolated from soil samples were identified as a relatives of Paenibacillus. A mutanase was purified to homogeneity from cultures of each, and the molecular masses of the purified enzymes were approximately 132, 141, and 141kDa, respectively. The corresponding three genes for mutanases were cloned by PCR using primers designed from each N-terminal amino acid sequence. Another mutanase-like gene from one strain was also cloned by PCR using primers designed from conserved amino acid sequences among known mutanases. Consequently, four mutanase-like genes were sequenced. The genes contained long open reading frames of 3411 to 3915bp encoding 1136 to 1304 amino acids. The deduced amino acid sequences of the mutanases showed relatively high similarity to those of a mutanase (E16590) from Bacillus sp. RM1 with 46.9% to 73.2% identity and an alpha-1,3-glucanase (AB248056) from Bacillus circulans KA-304 with 46.7% to 70.4% identity. Phylogenetic analysis based on the amino acid sequences of the enzymes showed bacterial mutanases form a new family between fungal mutanases (GH family 71) and Streptomycetes mycodextranases (GH family 87).
journal_name
Biochimiejournal_title
Biochimieauthors
Hakamada Y,Sumitomo N,Ogawa A,Kawano T,Saeki K,Ozaki K,Ito S,Kobayashi Tdoi
10.1016/j.biochi.2007.09.018subject
Has Abstractpub_date
2008-03-01 00:00:00pages
525-33issue
3eissn
0300-9084issn
1638-6183pii
S0300-9084(07)00261-1journal_volume
90pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract::Zika and Dengue viruses have attracted substantial attention from researchers in light of recent outbreaks of Dengue fever and increases in cases of congenital microcephaly in areas with Zika incidence. This review summarizes the current state of knowledge about Zika and Dengue proteases. These enzymes have several in...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2019.05.004
更新日期:2019-11-01 00:00:00
abstract::After 30 years of effort, the mammalian adenylate cyclase catalytic subunit has now been purified. It is a glycoprotein of 155 kDa, representing less than 0.01% of synaptosomal membrane protein. As measured in the presence of forskolin, its specific activity is 10-20 mumol of cAMP X mg-1 X min-1. The enzyme obtained i...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(87)90016-2
更新日期:1987-04-01 00:00:00
abstract::The aim of the present work was to establish the thermodynamic and functional differences in the protein-protein interactions between the components of the P450-dependent mitochondrial (mit) and microsomal (mic) monooxygenase systems using 12 different isoforms of cytochromes P450 and two redox partners, NADPH-depende...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2019.04.020
更新日期:2019-07-01 00:00:00
abstract::Streptomyces sp. 139 produces a novel exopolysaccharide (EPS) designated Ebosin which can bind IL-1R specifically and exhibits anti-rheumatic arthritis activity in vivo. With the Ebosin biosynthesis gene cluster (ste) consisting of 27 ORFs identified previously the focus of this study was to characterize the protein e...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2011.04.014
更新日期:2011-09-01 00:00:00
abstract::The thyroid hormones L-thyroxine and triiodo-L-thyronine have profound effects on postembryonic development of most vertebrates. Analysis of their action in mammals is vitiated by the exposure of the developing foetus to a number of maternal factors which do not allow one to specifically define the role of thyroid hor...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(99)80082-0
更新日期:1999-04-01 00:00:00
abstract::pH-Dependence of hydrolytic activity of trypsin has been studied in cationic reverse micellar system of cetyltrimethylammonium bromide (CTAB) in (50% v/v) chloroform/isooctane using a positively charged substrate N(alpha)-benzoyl-L-arginine ethyl ester (BAEE). The pH of the medium was varied from 4.0 to 8.5 with addit...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(99)80007-8
更新日期:1998-12-01 00:00:00
abstract::In man the brain represents about 2% of the body weight, but contains 25% of the body's cholesterol. Cholesterol itself does not cross the blood-brain barrier and is synthesised in situ. Excess cholesterol from brain is exported in the form of oxysterols, or metabolised to steroids, which in contrast to cholesterol ca...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2006.10.008
更新日期:2007-02-01 00:00:00
abstract::Theoretical and experimental investigations on the conformations of synthetic polytripeptides, with Gly-R2-R3 general repetitive sequence, are of great interest for the comprehension of the factors which contribute to the triple helix stabilization. Using appropriate molecules as models, we have investigated by the PC...
journal_title:Biochimie
pub_type: 杂志文章
doi:
更新日期:1981-11-01 00:00:00
abstract::Honeybee-plant relationships are based on a conditioning process in which olfactory (plant aroma) and gustatory cues (mainly nectars) are closely linked, leading to a selective foraging behaviour. Among crops dependent upon entomophilous cross-pollination, the sunflower has recently undergone extensive expansion due t...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(87)90186-6
更新日期:1987-06-01 00:00:00
abstract::The considerable affinity of tissue transglutaminase for heparin was the basis for use of heparin-based affinity matrices for enzyme purification. Interaction of transglutaminase with heparin might mimic the physiological binding to membrane heparan sulfates, accounting for the limited but significant fraction of enzy...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.01.012
更新日期:2005-06-01 00:00:00
abstract::Genome-editing technologies, in particular, CRISPR systems, are widely used for targeted regulation of gene expression and obtaining modified human and animal cell lines, plants, fungi, and animals with preassigned features. Despite being well described and easy to perform, the most common methods for construction and...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2019.09.003
更新日期:2019-12-01 00:00:00
abstract::Sialic acid, a nine-carbon sugar acid usually is present in the non-reducing terminal position of free oligosaccharides and glycoconjugates. Sialylated conjugates in mammals perform important roles in cellular recognition, signaling, host-pathogen interaction and neuronal development. Metabolism of sialylated conjugat...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2011.06.002
更新日期:2011-10-01 00:00:00
abstract::Sphingolipids are degraded by sphingomyelinase and ceramidase in the gut to ceramide and sphingosine, which may inhibit cell proliferation and induce apoptosis, and thus have anti-tumour effects in the gut. Although previous rodent studies including experiments on knockout mice indicate a role of neutral ceramidase in...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2007.03.009
更新日期:2007-08-01 00:00:00
abstract::Mammalian aminoacylase-1 (Acy1) participates in the breakdown of N-acetylated amino acids during intracellular protein catabolism. Acy1 is most abundantly expressed in the kidney tubular epithelium. Lately, Acy1 deficiency was identified in children with increased urinary excretion of several N-acetylamino acids. Here...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2007.12.006
更新日期:2008-05-01 00:00:00
abstract::Peptidase family S46 consists of two types of dipeptidyl-peptidases (DPPs), DPP7 and DPP11, which liberate dipeptides from the N-termini of polypeptides along with the penultimate hydrophobic and acidic residues, respectively. Their specificities are primarily defined by a single amino acid residue, Gly673 in DPP7 and...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2017.10.015
更新日期:2018-04-01 00:00:00
abstract::With the relation between chemical structure and pharmacological activity as a guide, we have been for some time synthetizing a wide range of beta-amino-ketone derivatives. One of them, 2-(4-methyl-1-piperazinylmethyl) acrylophenone, MPMAP, possesses antimicrotubular activities. This product inhibits 50% of the microt...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(84)90083-x
更新日期:1984-06-01 00:00:00
abstract::The number of available genomic sequences is growing very fast, due to the development of massive sequencing techniques. Sequence identification is needed and contributes to the assessment of gene and species evolutionary relationships. Automated bioinformatics tools are thus necessary to carry out these identificatio...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2007.08.006
更新日期:2008-04-01 00:00:00
abstract::Since 2012, the interest for TMEM165 increased due to its implication in a rare genetic human disease named TMEM165-CDG (Congenital Disorder(s) of Glycosylation). TMEM165 is a Golgi localized protein, highly conserved through evolution and belonging to the uncharacterized protein family 0016 (UPF0016). Although the pr...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2019.07.016
更新日期:2019-10-01 00:00:00
abstract::The product of the sex-determining gene SRY is a member of the HMG box containing protein superfamily. The HMG box is a DNA-binding domain of about 80 amino acids shared by many proteins with diverse functions. It seems that the functions of the full length protein are restricted to the HMG box but their molecular bas...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(94)90033-7
更新日期:1994-01-01 00:00:00
abstract::Ceramide synthases (CerSs) are key enzymes in the biosynthesis of ceramides and display a group of at least six different isoenzymes (CerS1-6). Ceramides itself are bioactive molecules. Ceramides with different N-acyl side chains (C(14:0)-Cer - C(26:0)-Cer) possess distinct roles in cell signaling. Therefore, the sele...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2011.09.007
更新日期:2012-02-01 00:00:00
abstract::The redox homeostasis of cytoplasm is maintained by a series of disulfide exchange reactions mediated by proteins belonging to the thioredoxin superfamily. Thioredoxin and thioredoxin reductase, being the major members of the family, play a key role in oxidative stress response of Staphylococcus aureus. In this report...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2019.01.019
更新日期:2019-05-01 00:00:00
abstract::Bikunin (Bk) is a Künitz-type serine proteinase inhibitor, which occurs in human plasma, mainly as covalent complexes with one or two of the three peptide heavy chains. The leading member of this glycoprotein family is inter-alpha-inhibitor (I alpha I), which consists of two heavy chains (H1 and H2) linked to Bk. Bk c...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(03)00066-x
更新日期:2003-01-01 00:00:00
abstract::The complete amino acid sequences of 3 proteins from the 50S subunit of Bacillus stearothermophilus ribosomes were determined by N-terminal sequence analysis and by sequencing of overlapping fragments obtained from enzymatic digestions and chemical cleavages. The proteins BstL28, BstL33 and BstL34, named according to ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(91)90126-l
更新日期:1991-07-01 00:00:00
abstract::Fanconi anemia (FA) is a rare and complex inherited blood disorder of the child. At least 15 genes are associated with the disease. The highest frequency of mutations belongs to groups A, C and G. Genetic instability and cytokine hypersensitivity support the selection of leukemic over non-leukemic stem cells. FA cellu...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.06.006
更新日期:2013-10-01 00:00:00
abstract::In around 10% of SARS-CoV-2 infected patients, coronavirus disease-2019 (Covid-19) symptoms are complicated with a severe lung damage called Acute Respiratory Distress Syndrome (ARDS), which is often lethal. ARDS is mainly associated with an uncontrolled overproduction of immune cells and cytokines, called "cytokine s...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2020.09.003
更新日期:2020-12-01 00:00:00
abstract::Labeling of 21-kDa material was observed when bovine brain soluble fraction was incubated with [adenylate-32P]NAD+ in the presence of GTP. The 21-kDa substrate, slightly smaller than C3 substrate in size, was labeled even without C3 exoenzyme. GTP could be replaced by nucleoside triphosphates other than ATP while ATP ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(97)86150-0
更新日期:1997-07-01 00:00:00
abstract::Influenza virus-associated RNA polymerase is composed of one molecule each of three viral P proteins and carries the complete activity of capped RNA-primed vRNA-directed transcription. The RNA polymerase holoenzyme also carries capped RNA endonuclease to generate capped oligonucleotide primers for transcription and 3'...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(97)86735-1
更新日期:1996-01-01 00:00:00
abstract::HIV-1 reverse transcriptase uses the host tRNA3(Lys) as a primer for the synthesis of the minus DNA strand. The first event in viral replication is thus the annealing of tRNA to the primer binding site (PBS) in the 5' UTR of the viral RNA. This event requires a major RNA rearrangement which is chaperoned by the viral ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2007.01.016
更新日期:2007-10-01 00:00:00
abstract::In this work, were studied the conditions of erucic acid (cis-docosenoic, n-9) shortening by using Rat liver mitochondrial preparations which were incubated in vitro with [14-14C] erucic acid (22:1), with inhibitors of the respiratory chain (rotenone, cyanide) or not, with activators of either the shortening reaction ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(82)80352-0
更新日期:1982-10-01 00:00:00
abstract::It has been shown that two Proteus morganii strains produce an inducible cephalosporinase. No significative difference was shown between them: they present the same isoelectric poit: 8,3 and very similar kinetic constants. The parameter tau, proportional to the half life of antibiotic at low concentration, in presence...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(76)80086-7
更新日期:1976-11-13 00:00:00