当前位置： SCI文献检索 > BIOCHIMIE期刊下所有文献 > A multi-functional enzyme with RNA polymerase and RNase activities: molecular anatomy of influenza virus RNA polymerase.

A multi-functional enzyme with RNA polymerase and RNase activities: molecular anatomy of influenza virus RNA polymerase.

Abstract：

:Influenza virus-associated RNA polymerase is composed of one molecule each of three viral P proteins and carries the complete activity of capped RNA-primed vRNA-directed transcription. The RNA polymerase holoenzyme also carries capped RNA endonuclease to generate capped oligonucleotide primers for transcription and 3'-to-5' exonuclease to remove erroneously polymerized nucleotides at nascent RNA 3' termini prior to the addition of correct substrates. PB1 is the core subunit for not only RNA synthesis but also the assembly of PB2 and PA into the holoenzyme complex, while PB2 plays a key role in capped RNA cleavage. The transcriptase is converted into the RNA replicase with the full activity of replication, ie vRNA-directed cRNA synthesis and cRNA-directed vRNA synthesis, after interaction with an as yet unidentified host factor(s).

journal_name

Biochimie

journal_title

Biochimie

authors

Ishihama A

doi

10.1016/s0300-9084(97)86735-1

subject

Has Abstract

pub_date

1996-01-01 00:00:00

pages

1097-102

issue

11-12

eissn

0300-9084

issn

1638-6183

pii

S0300-9084(97)86735-1

journal_volume

78

pub_type

杂志文章,评审

相关文献

BIOCHIMIE文献大全
  • Sensitive to apoptosis gene protein regulates ionizing radiation-induced apoptosis.

    abstract：:Organisms exposed to ionizing radiation (IR) undergo increases in the production of reactive oxygen species (ROS), which are determinant components in the induction of apoptosis. Sensitive to apoptosis gene (SAG) encodes a redox-inducible and apoptosis-protective antioxidant protein. This report demonstrates that the ...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2010.09.020

    authors： Kim SY,Yang ES,Lee YS,Lee J,Park JW

    更新日期：2011-02-01 00:00:00

  • Identification and characterization of a novel spermidine/spermine acetyltransferase encoded by gene ste26 from Streptomyces sp. 139.

    abstract：:Streptomyces sp. 139 produces a novel exopolysaccharide (EPS) designated Ebosin which can bind IL-1R specifically and exhibits anti-rheumatic arthritis activity in vivo. With the Ebosin biosynthesis gene cluster (ste) consisting of 27 ORFs identified previously the focus of this study was to characterize the protein e...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2011.04.014

    authors： Bai L,Chang M,Shan J,Jiang R,Zhang Y,Zhang R,Li Y

    更新日期：2011-09-01 00:00:00

  • Purification of the catalytic subunit of adenylate cyclase in vertebrates: state of the art in 1987.

    abstract：:After 30 years of effort, the mammalian adenylate cyclase catalytic subunit has now been purified. It is a glycoprotein of 155 kDa, representing less than 0.01% of synaptosomal membrane protein. As measured in the presence of forskolin, its specific activity is 10-20 mumol of cAMP X mg-1 X min-1. The enzyme obtained i...

    journal_title：Biochimie

    pub_type： 杂志文章,评审

    doi：10.1016/0300-9084(87)90016-2

    authors： Monneron A,d'Alayer J,Coussen F

    更新日期：1987-04-01 00:00:00

  • Biochemical characterization, stability studies and N-terminal sequence of a bi-functional inhibitor from Phaseolus aureus Roxb. (Mung bean).

    abstract：:Herein, we report the purification and biochemical characterization of a novel bi-functional protein proteinase/amylase inhibitor from the dietary leguminous pulse Phaseolus aureus Roxb. (Vigna radiata L.) by means of acetic acid precipitation, salt fractionation, ion-exchange chromatography (DEAE-cellulose) and affin...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2005.05.007

    authors： Haq SK,Atif SM,Khan RH

    更新日期：2005-12-01 00:00:00

  • Contact-dependent inhibition of growth of normal diploid human fibroblasts by plasma membrane glycoproteins.

    abstract：:Homeostasis in vivo is maintained by a highly complex network of positive and negative signals. At the cellular level, this regulatory microenvironment can be divided, in a simplified fashion, into two major compartments: the humoral compartment, including compounds such as hormones, growth factors and nutrients, and ...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/0300-9084(88)90301-x

    authors： Wieser RJ,Janik-Schmitt B,Renauer D,Schäfer A,Heck R,Oesch F

    更新日期：1988-11-01 00:00:00

  • Nucleotide sugar transporters: biological and functional aspects.

    abstract：:The Golgi apparatus serves as the major site of glycosylation reactions. Nucleotide sugars which are substrates of the Golgi localized glycosyltransferases are synthesized in the cytoplasm (cell nucleus in case of CMP-sialic acid) and must be transported into the compartment lumen. This transport function is carried o...

    journal_title：Biochimie

    pub_type： 杂志文章,评审

    doi：10.1016/s0300-9084(01)01322-0

    authors： Gerardy-Schahn R,Oelmann S,Bakker H

    更新日期：2001-08-01 00:00:00

  • Further studies of the glucomannan from Aloe vahombe (liliaceae). II. Partial hydrolyses and NMR 13C studies.

    abstract：:The polysaccharide from Aloe vahombe (lilaceae) was submitted to partial hydrolyses by sulfuric, oxalic and phosphoric acid. Some oligosaccharides were isolated and investigated by chemical and 13C NMR spectroscopic methods. Their structure was determined. The results prove unambiguously that in the analyzed material ...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/0300-9084(84)90151-2

    authors： Radjabi-Nassab F,Ramiliarison C,Monneret C,Vilkas E

    更新日期：1984-07-01 00:00:00

  • Insights on calcium-independent phospholipid membrane damage by Lys49-PLA2 using tryptophan scanning mutagenesis of bothropstoxin-I from Bothrops jararacussu.

    abstract：:Bothropstoxin-I (BthTx-I) is a homodimeric Lys49-PLA2 from the venom of the snake Bothrops jararacussu, which lacks hydrolytic activity against phospholipid substrates, yet permeabilizes membranes by a Ca2+-independent mechanism. The interaction of the BthTx-I with model membranes has been studied by intrinsic tryptop...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2007.10.012

    authors： Ferreira TL,Ruller R,Chioato L,Ward RJ

    更新日期：2008-09-01 00:00:00

  • [Biosynthesis of phorcabiline, blue-green biliary pigment of Actias selene (Lepidoptera, Attacidae)].

    abstract：:The blue-green bile pigments of Actias selene (Attacidae) have been investigated at different stages of its development. Coproporphyrinogen-14-C, protoporphyrin-IX3-H, and pterobilin-14-C, injected to larvae are metabolised into phorcabilin I, the main neopterobilin in this animal. It is concluded that phorcabilin I i...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/s0300-9084(75)80313-0

    authors： Choussy M,Barbier M,Vuillaume M

    更新日期：1975-01-01 00:00:00

  • A role for calcium in Bcl-2 action?

    abstract：:Changes in the cytosolic Ca(2+) concentration ([Ca(2+)](c)) translate a variety of extracellular signals into widely diverse intracellular effects, ranging from secretion to movement, proliferation and also cell death. As regards the last one, it has long been known that large [Ca(2+)](c) increases lead cells to death...

    journal_title：Biochimie

    pub_type： 杂志文章,评审

    doi：10.1016/s0300-9084(02)01373-1

    authors： Pinton P,Ferrari D,Rapizzi E,Di Virgilio F,Pozzan T,Rizzuto R

    更新日期：2002-02-01 00:00:00

  • Eukaryotic extracellular catalase-peroxidase from Magnaporthe grisea - Biophysical/chemical characterization of the first representative from a novel phytopathogenic KatG group.

    abstract：:All phytopathogenic fungi have two catalase-peroxidase paralogues located either intracellularly (KatG1) or extracellularly (KatG2). Here, for the first time a secreted bifunctional, homodimeric catalase-peroxidase (KatG2 from the rice blast fungus Magnaporthe grisea) has been produced heterologously with almost 100% ...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2011.09.020

    authors： Zámocký M,Droghetti E,Bellei M,Gasselhuber B,Pabst M,Furtmüller PG,Battistuzzi G,Smulevich G,Obinger C

    更新日期：2012-03-01 00:00:00

  • The recA-recBCD dependent recombination pathways of Serratia marcescens and Proteus mirabilis in Escherichia coli: functions of hybrid enzymes and hybrid pathways.

    abstract：:The physical maps of cloned recBCD gene regions of Serratia marcescens and Proteus mirabilis were correlated to genes located in this region. The genes thyA, recC, recB, recD and argA were organized as in Escherichia coli. The 3 rec genes code for the 3 different subunits of the RecBCD enzyme and produced enzymes prom...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/0300-9084(91)90104-9

    authors： Rinken R,de Vries J,Weichenhan D,Wackernagel W

    更新日期：1991-04-01 00:00:00

  • Primer on genes encoding enzymes in sialic acid metabolism in mammals.

    abstract：:Sialic acid, a nine-carbon sugar acid usually is present in the non-reducing terminal position of free oligosaccharides and glycoconjugates. Sialylated conjugates in mammals perform important roles in cellular recognition, signaling, host-pathogen interaction and neuronal development. Metabolism of sialylated conjugat...

    journal_title：Biochimie

    pub_type： 杂志文章,评审

    doi：10.1016/j.biochi.2011.06.002

    authors： Wickramasinghe S,Medrano JF

    更新日期：2011-10-01 00:00:00

  • Recognition of cell surface acceptors by two human alpha-2,6-sialyltransferases produced in CHO cells.

    abstract：:The action of sialyltransferases (STs) on cell surface glycoconjugates is a key process in shaping cell phenotype in a variety of cells mostly involved in migratory and adhesive pathways. The factors determining cell-specific pattern of glycosylation are so far poorly understood. Most STs are resident proteins of the ...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/s0300-9084(03)00080-4

    authors： Donadio S,Dubois C,Fichant G,Roybon L,Guillemot JC,Breton C,Ronin C

    更新日期：2003-03-01 00:00:00

  • Conformational change at the active center of serine-proteases upon their binding to alpha 2-macroglobulin.

    abstract：:The inhibition rates and spectral characteristics of 2 probes specific for the active-site serine residue of proteases were examined for evidence of conformational change of the proteases upon their binding to alpha 2-macroglobulin (alpha 2M). Elastase, chymotrypsin, trypsin, and plasmin were reacted with (7-nitrobenz...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/0300-9084(89)90004-7

    authors： Martini JL,Pochon F

    更新日期：1989-03-01 00:00:00

  • Inactivation of proteases and esterases by halomethylated derivatives of dihydrocoumarins.

    abstract：:A series of halomethylated derivatives of dihydrocoumarins has been found to inhibit irreversibly proteases and esterases. alpha-Chymotrypsin, subtilish, elastase are rapidly inactivated in the presence of these compounds, while trypsin, kallicrein, papain are inhibited more slowly. Esterases like acetylcholinesterase...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/s0300-9084(77)80140-5

    authors： Béchet JJ,Dupaix A,Roucous C,Bonamy AM

    更新日期：1977-01-01 00:00:00

  • Metabolic impacts of altering meal frequency and timing - Does when we eat matter?

    abstract：:Obesity prevalence continues to rise throughout the developed world, as a result of positive energy balance and reduced physical activity. At present, there is still a perception within the general community, and amongst some nutritionists, that eating multiple small meals spaced throughout the day is beneficial for w...

    journal_title：Biochimie

    pub_type： 杂志文章,评审

    doi：10.1016/j.biochi.2015.07.025

    authors： Hutchison AT,Heilbronn LK

    更新日期：2016-05-01 00:00:00

  • Conformational stability and energetics of Plasmodium falciparum glutaredoxin.

    abstract：:Glutaredoxins (Grxs), redox-active proteins with a typical -CPYC motif at their active sites, are involved in redox-regulatory processes and antioxidant defenses. The human malarial parasite Plasmodium falciparum possess a classical glutaredoxin (PfGrx) as well as a number of Grx-like proteins. In the present study, w...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2009.12.003

    authors： Tripathi T,Röseler A,Rahlfs S,Becker K,Bhakuni V

    更新日期：2010-03-01 00:00:00

  • An empirical energy function for structural assessment of protein transmembrane domains.

    abstract：:Knowing the structure of a protein is essential to characterize its function and mechanism at the molecular level. Despite major advances in solving structures experimentally, most membrane protein native conformations remain unknown. This lack of available structures, along with the physical constraints imposed by th...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2015.05.018

    authors： Postic G,Ghouzam Y,Gelly JC

    更新日期：2015-08-01 00:00:00

  • Compression and genetic sequence analysis.

    abstract：:A novel approach to genetic sequence analysis is presented. This approach, based on compression of algorithms, has been launched simultaneously by Grumbach and Tahi, Milosavljevic and Rivals. To reduce the description of an object, a compression algorithm replaces some regularities in the description by special codes....

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/0300-9084(96)84763-8

    authors： Rivals E,Dauchet M,Delahaye JP,Delgrange O

    更新日期：1996-01-01 00:00:00

  • N-terminal transmembrane domain of lipase LipA from Pseudomonas protegens Pf-5: A must for its efficient folding into an active conformation.

    abstract：:LipA from Pseudomonas protegens Pf-5 has been proven not to be secreted into the extracytoplasmic space, proposing that it is a membrane protein in virtue of its N-terminal transmembrane domain predicted by the TMHMM 2.0. However, LipA was confirmed to be an intracellular protein through determining the effects of lip...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2014.07.007

    authors： Zha D,Zhang H,Zhang H,Xu L,Yan Y

    更新日期：2014-10-01 00:00:00

  • A review of nutritional effects on fat composition of animal products with special emphasis on n-3 polyunsaturated fatty acids.

    abstract：:The fatty acid composition of animal products (eggs, milk and meat) is the reflect of both the tissue fatty acid biosynthesis and the fatty acid composition of ingested lipids. This relationship is stronger in monogastrics (pigs, poultry and rabbits) than in ruminants, where dietary fatty acids are hydrogenated in the...

    journal_title：Biochimie

    pub_type： 杂志文章,评审

    doi：10.1016/j.biochi.2010.02.027

    authors： Kouba M,Mourot J

    更新日期：2011-01-01 00:00:00

  • Characterization of HU-like protein from Bifidobacterium longum.

    abstract：:A HU-like protein (HBl) of Bifidobacterium longum was purified and characterized. HBl is heat-stable and acid-resistant, and has a molecular weight of about 9.1 kDa as estimated by its mobility on electrophoresis. HBl is intermediate in basicity (pI 9.8) between the HU-1 and HU-2 proteins of Escherichia coli, and is d...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/0300-9084(90)90074-q

    authors： Goshima N,Kano Y,Imamoto F

    更新日期：1990-04-01 00:00:00

  • Plasmodium falciparum SERA protein peptide analogues having short helical regions induce protection against malaria.

    abstract：:Three Plasmodium falciparum serine repeat antigen (SERA) protein peptides were studied by NMR and structure calculations being done in 70:30 water:trifluoroethanol solution. Peptide 22834 was shown to be immunogenic and protective against malaria in Aotus monkeys, whilst native peptide 6737 and its analogue 14096 did ...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/s0300-9084(03)00136-6

    authors： Cubillos M,Alba MP,Bermúdez A,Trujillo M,Patarroyo ME

    更新日期：2003-07-01 00:00:00

  • The application of nuclear magnetic resonance spectroscopy to the study of natural and model membranes.

    abstract：:This review article outlines some potentials and limits of the recent application of high resolution Nuclear Magnetic Resonance technique--coupled to the Fourier transformation methods--to the study of biological membranes. Molecular arrangement and dynamical structure characters can be assessed at the level of indivi...

    journal_title：Biochimie

    pub_type： 杂志文章,评审

    doi：10.1016/s0300-9084(75)80333-6

    authors： Podo F

    更新日期：1975-01-01 00:00:00

  • Luteolin 8-C-β-fucopyranoside inhibits invasion and suppresses TPA-induced MMP-9 and IL-8 via ERK/AP-1 and ERK/NF-κB signaling in MCF-7 breast cancer cells.

    abstract：:Matrix metalloproteinase 9 (MMP-9) and interleukin-8 (IL-8) play major roles in tumor progression and invasion of breast cancer cells. The present study was undertaken to investigate the inhibitory mechanism of cell invasion by luteolin 8-C-β-fucopyranoside (named as LU8C-FP), a C-glycosylflavone, in human breast canc...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2013.07.021

    authors： Park SH,Kim JH,Lee DH,Kang JW,Song HH,Oh SR,Yoon DY

    更新日期：2013-11-01 00:00:00

  • Type I interferon gene expression: differential expression of IFN-A genes induced by viruses and double-stranded RNA.

    abstract：:The family of interferon regulatory transcription factors (IRF) participates in the virus-induced and dsRNA-stimulated transcriptional regulation of either type I IFN genes or a definite set of genes which can also be activated by IFN. In this review, we place emphasis on the role of IRF-3 that associates with the coa...

    journal_title：Biochimie

    pub_type： 杂志文章,评审

    doi：10.1016/s0300-9084(99)80021-2

    authors： Bragança J,Civas A

    更新日期：1998-08-01 00:00:00

  • The triazatruxene derivative azatrux binds to the parallel form of the human telomeric G-quadruplex under molecular crowding conditions: biophysical and molecular modeling studies.

    abstract：:The present study has employed a combination of spectroscopic, calorimetric and computational methods to explore the binding of the three side-chained triazatruxene derivative, termed azatrux, to a human telomeric G-quadruplex sequence, under conditions of molecular crowding. The binding of azatrux to the tetramolecul...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2011.05.017

    authors： Petraccone L,Fotticchia I,Cummaro A,Pagano B,Ginnari-Satriani L,Haider S,Randazzo A,Novellino E,Neidle S,Giancola C

    更新日期：2011-08-01 00:00:00

  • PPAR agonists, - Could tissue targeting pave the way?

    abstract：:Over the last couple of decades, the PPAR family of transcription factors has received much attention from the pharmaceutical industry due to their profound ability to improve glucose and lipid metabolism upon agonist activation. However, more recently the interest in these nuclear receptors has faded because several ...

    journal_title：Biochimie

    pub_type： 杂志文章,评审

    doi：10.1016/j.biochi.2016.10.017

    authors： Bugge A,Holst D

    更新日期：2017-05-01 00:00:00

  • Cytoplasmic peptidoglycan intermediate levels in Staphylococcus aureus.

    abstract：:Intracellular cytoplasmic peptidoglycan (PG) intermediate levels were determined in Staphylococcus aureus during log-phase growth in enriched media. Levels of UDP-linked intermediates were quantitatively determined using ion pairing LC-MS/MS in negative mode, and amine intermediates were quantitatively determined ster...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2015.11.017

    authors： Vemula H,Ayon NJ,Gutheil WG

    更新日期：2016-02-01 00:00:00