Abstract:
:The product of the sex-determining gene SRY is a member of the HMG box containing protein superfamily. The HMG box is a DNA-binding domain of about 80 amino acids shared by many proteins with diverse functions. It seems that the functions of the full length protein are restricted to the HMG box but their molecular basis remains to be determined. We have summarized here the properties of this binding domain described so far in the literature and, using a synthetic peptide mimicking the DNA binding domain (SRY80), we have confirmed the existence of DNA minor groove contacts with this domain. Using intrinsic fluorescence of the tryptophane, the interaction between SRY80 and the putative target sequence AACAAAT was also quantified. In conclusion, we also consider the possible putative action of SRY to fulfill its role in sex determination.
journal_name
Biochimiejournal_title
Biochimieauthors
Soullier S,Poulat F,Boizet-Bonhoure B,Calas B,Bennes R,Heitz F,Berta Pdoi
10.1016/0300-9084(94)90033-7subject
Has Abstractpub_date
1994-01-01 00:00:00pages
1075-81issue
10-11eissn
0300-9084issn
1638-6183pii
0300-9084(94)90033-7journal_volume
76pub_type
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