Abstract:
:Crystals of flavoenzyme L-lactate oxidase from Aerococcus viridans (LOX) have been obtained that diffract to 3.0 A resolution (P2(1)2(1)2(1), a = 118.4 A, b = 138.4 A, c = 194.6 A). Crystallographic studies suggest that the enzyme may exist as an octameric form with non-crystallographic two- and four-fold axes in the center of the octamer. The four-fold axis makes the tetramer tight, and the tetramers lie upon one another by the two-fold axis.
journal_name
Biochimiejournal_title
Biochimieauthors
Morimoto Y,Yorita K,Aki K,Misaki H,Massey Vdoi
10.1016/s0300-9084(98)80072-2subject
Has Abstractpub_date
1998-04-01 00:00:00pages
309-12issue
4eissn
0300-9084issn
1638-6183pii
S0300-9084(98)80072-2journal_volume
80pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract::Lipidomics is a subspecialty of metabolomics that focuses on water insoluble metabolites that form membrane barriers. Most lipidomic databases catalog lipids from common model organisms, like humans or Escherichia coli. However, model organisms' lipid profiles show surprisingly little overlap with those of specialized...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2012.08.012
更新日期:2013-01-01 00:00:00
abstract::Antimicrobials targeting the fatty acid synthesis (FASII) pathway are being developed as alternative treatments for bacterial infections. Emergence of resistance to FASII inhibitors was mainly considered as a consequence of mutations in the FASII target genes. However, an alternative and efficient anti-FASII resistanc...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2017.07.004
更新日期:2017-10-01 00:00:00
abstract::Three trends are seen in the organization of ribosomal DNA genes during evolution: 1) gradual separation and separability of the regulation of transcription of 5S and larger subunit rRNAs; 2) retention of a transcription unit containing both large and small rRNAs; and 3) clustering of genes for both 5S and 18S-28S rDN...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(91)90042-y
更新日期:1991-06-01 00:00:00
abstract::Experimental evidence accumulated to date by several research groups indicates that antisense oligodeoxynucleotides targeted against messenger RNA (mRNA) sequences located downstream of the initiation codon fail to inhibit the translation of this mRNA unless the hybrid is cleaved by RNase H. It has previously been sho...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(93)90032-n
更新日期:1993-01-01 00:00:00
abstract::Betaine aldehyde dehydrogenase from the human opportunistic pathogen Pseudomonas aeruginosa (PaBADH) catalyzes the irreversible, NAD(P)(+)-dependent oxidation of betaine aldehyde, producing glycine betaine, an osmoprotectant. PaBADH participates in the catabolism of choline and likely in the defense against the osmoti...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.09.022
更新日期:2011-02-01 00:00:00
abstract::Classic studies suggested that the common mammalian sialic acid N-glycolylneuraminic acid (Neu5Gc) is an oncofetal antigen in humans, being immunogenic in adult humans and yet apparently expressed in human fetuses and tumors. We and others have recently found that the human deficiency of Neu5Gc can be explained by an ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(01)01309-8
更新日期:2001-07-01 00:00:00
abstract::The periplasmic histidine permease of Salmonella typhimurium is composed of a soluble histidine-binding protein and three membrane-bound components. These latter are produced in very small amounts and only two, the Q and the P protein, have been previously identified. This paper describes the construction of a plasmid...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(85)80242-x
更新日期:1985-01-01 00:00:00
abstract::Surfactants carrying either a hydrocarbon or a fluorocarbon alkyl chain have been synthesized. The polar head was either tris(hydroxymethyl)acrylamidomethane (THAM), telomerized THAM, or a glycosylated THAM moiety. The aqueous solubility of some of these molecules was increased by oxidizing to a sulfoxide the thioethe...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(00)80017-6
更新日期:1998-05-01 00:00:00
abstract::Snake venom from Agkistrodon acutus consists of a number of compounds which may potentially be used as drugs. However, it is hard to obtain enough pure protein for drug development. Recently, we reported expression and purification of a novel recombinant fibrinogenase which was named rFII. Here we reported for the fir...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2008.10.007
更新日期:2009-02-01 00:00:00
abstract::Haloalkane dehalogenases are known as bacterial enzymes cleaving a carbon-halogen bond in halogenated compounds. Here we report the first biochemically characterized non-microbial haloalkane dehalogenase DspA from Strongylocentrotus purpuratus. The enzyme shows a preference for terminally brominated hydrocarbons and e...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.07.025
更新日期:2013-11-01 00:00:00
abstract::Natural bovine seminal RNase possesses a potent antitumor action. We have mutagenized monomeric bovine pancreatic RNase A, devoid of any cytotoxic action, to insert residues present at corresponding positions in the subunit of dimeric, antitumor, seminal RNase. Like naturally dimeric seminal RNase, the mutant dimeric ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(00)88887-2
更新日期:1998-11-01 00:00:00
abstract::Interleukin-17 (IL-17) is produced exclusively by activated T cells and neutrophils, and stimulates osteoclastic bone resorption via osteoblasts by inducing the expression of "receptor activator of NF-kappaB (RANK) ligand" (RANKL). However, the direct effects of IL-17 on the differentiation of osteoclast precursors in...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2009.12.011
更新日期:2010-04-01 00:00:00
abstract::L-aromatic aminoacid decarboxylase has been purified more than thousand times from homogenates of rat brain, in several steps : centrifugation, DEAE-cellulose, CM cellulose, hydroxylapatite, DEAE sephadex. Its properties have been studied, most of them on an intermediate fraction of the purification, because of the in...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(75)80356-7
更新日期:1975-01-01 00:00:00
abstract::The gene encoding the enzyme tyrosyl tRNA synthetase from Bacillus stearothermophilus has been systematically altered using synthetic oligonucleotides as mutagens. The construction of mutations has been facilitated by using strains of bacteria defective in mismatch repair and also by utilising a genetic marker in the ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(85)80161-9
更新日期:1985-07-01 00:00:00
abstract::The chromatin of shrimp hepatopancreas has been extracted from isolated nuclei and characterized. Nuclei were prepared in the presence of Cu++ and phenyl methyl sulfonyl fluoride in order to inhibit the nuclease and protease activities throughout the different purification steps. The purified nuclei are heterogenous i...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(79)80133-9
更新日期:1979-01-01 00:00:00
abstract::After the assessment of the antitumor activity of the anthracycline pigments, the S peucetius group of metabolites was discovered and eventually doxorubicin, a major anticancer agent of established clinical usefulness was developed in the early seventies. A second generation of compounds followed, represented mainly b...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(98)80003-5
更新日期:1998-03-01 00:00:00
abstract::In this study, peptidomics and genomics analyses were used to study antimicrobial peptides from the skin of Hylarana spinulosa. Twenty-nine different antimicrobial peptide precursors were characterized from the skin of H. spinulosa, which produce 23 mature antimicrobial peptides belonging to 12 different families. To ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.09.002
更新日期:2013-12-01 00:00:00
abstract::Our previous researches showed that nitronyl nitroxyl derivatives, NNP and NNVP were good anti-oxidants and provided radioprotective effects in C6 cells. The objective of the present study is to investigate the possible antiviral effects and underlying pharmacological of the two nitronyl nitroxide radicals against CVB...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.05.015
更新日期:2012-09-01 00:00:00
abstract::The complete amino acid sequences of 3 proteins from the 50S subunit of Bacillus stearothermophilus ribosomes were determined by N-terminal sequence analysis and by sequencing of overlapping fragments obtained from enzymatic digestions and chemical cleavages. The proteins BstL28, BstL33 and BstL34, named according to ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(91)90126-l
更新日期:1991-07-01 00:00:00
abstract::Thyroid peroxidase (TPO) is the key enzyme involved in thyroid hormone synthesis. Autoantibodies to TPO (TPOAbs) are a hallmark of autoimmune thyroid disease (AITD). Here, we highlight recent progress over several years in understanding TPO biochemistry and function in various pathologies. TPO undergoes complex post-t...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2019.02.003
更新日期:2019-05-01 00:00:00
abstract::Complexes of purified 40S ribosomal subunits and initiation factor 3 from rabbit reticulocytes were crosslinked using the reversible protein crosslinking reagent, 2-iminothiolane, under conditions shown previously to lead to the formation of dimers between 40S proteins but not higher multimers. The activity of both th...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(83)80062-5
更新日期:1983-07-01 00:00:00
abstract::Canine S100A12 (cS100A12) is a calcium-binding protein of the S100 superfamily of EF-hand proteins, and its expression is restricted to neutrophils and monocytes. Interaction of S100A12 with the receptor for advanced glycation end products (RAGE) has been suggested to play a central role in inflammation. Moreover, S10...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.08.007
更新日期:2010-12-01 00:00:00
abstract::The effect of 7-chloro-4-nitrobenzofurazan on yeast 3-phosphoglycerate kinase causes a modification of one tyrosyl residue concomitantly with a total loss of activity of the enzyme. The modification is not accompanied by any significant conformational change. A total protection against inactivation is observed with th...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(79)80164-9
更新日期:1979-01-01 00:00:00
abstract::Previous reports on the carbohydrate specificities of Amaranthus caudatus lectin (ACL) and peanut agglutinin (PNA, Arachis hypogea) indicated that they share the same specificity for the Thomsen-Friedenreich (T(alpha), Galbeta1-3GalNAcalpha1-Ser/Thr) glycotope, but differ in monosaccharide binding--GalNAc>Gal (inactiv...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2008.08.001
更新日期:2008-11-01 00:00:00
abstract::Mitochondrial function requires genes encoded in both mitochondrial and nuclear genomes. Tfam, the activator of mammalian mitochondrial transcription, is encoded in the nucleus and its expression has been shown in in vitro studies to be controlled by nuclear respiratory factors NRF-1 and NRF-2. In order to understand ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(99)00223-0
更新日期:1999-10-01 00:00:00
abstract::Starting from total pancreatic mRNAs, turkey pancreatic lipase (TPL) cDNA was synthesized by RT-PCR and cloned into the PGEM-T vector. Amino acid sequence of the TPL is compared to that of human pancreatic lipase (HPL). A 3-D structure model of TPL was built using the 3-D structure of HPL as template, given the high a...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2006.05.022
更新日期:2006-10-01 00:00:00
abstract::Bacillus subtilis strain SO113 secretes a pectate lyase which is produced during the exponential death phase of growth, just before sporulation. This extracellular pectate lyase, which produces unsaturated products from polygalacturonate, was purified 35-fold from the culture supernatant of Bacillus subtilis by a CM S...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(90)90053-j
更新日期:1990-09-01 00:00:00
abstract::Interferons (IFNs) were considered for the treatment of patients with multiple sclerosis (MS) after the demonstration, based on small studies, of the efficacy of type IFN beta in decreasing the frequency of exacerbations in relapsing-remitting multiple sclerosis when administered intrathecally, subcutaneously, or intr...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2007.03.016
更新日期:2007-06-01 00:00:00
abstract::We have recently reported that α(2)β(1) and α(1)β(1) isozymes of Na(+)/K(+)-ATPase (NKA) are localized in the caveolae whereas only the α(1)β(1) isozyme of NKA is localized in the non-caveolae fraction of pulmonary artery smooth muscle cell membrane. It is well known that different isoforms of NKA are regulated differ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2011.12.020
更新日期:2012-04-01 00:00:00
abstract::Diacylglycerol kinase (DGK) is a lipid-metabolizing enzyme that phosphorylates diacylglycerol (DG) to produce phosphatidic acid (PA). DGKδ is highly expressed in the skeletal muscle, and a decrease in DGKδ expression increases the severity of type 2 diabetes. However, the role of DGKδ in myogenic differentiation is st...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2018.05.017
更新日期:2018-08-01 00:00:00