Conformational dynamics and enzyme activity.

abstract：:In this work, were studied the conditions of erucic acid (cis-docosenoic, n-9) shortening by using Rat liver mitochondrial preparations which were incubated in vitro with [14-14C] erucic acid (22:1), with inhibitors of the respiratory chain (rotenone, cyanide) or not, with activators of either the shortening reaction ...

journal_title：Biochimie

authors： Clouet P,Henninger C,Bezard J

更新日期：1982-10-01 00:00:00

abstract：:Conjugated linoleic acids (CLA) are positional and geometric dienoic isomers of linoleic acid. Dietary CLA supplementation leads to a drop in fat mass in various species, including in humans. The t10,c12-CLA isomer is responsible for this anti-obesity effect. The reduction of fat mass is especially dramatic in the mou...

journal_title：Biochimie

authors： Poirier H,Niot I,Clément L,Guerre-Millo M,Besnard P

更新日期：2005-01-01 00:00:00

abstract：:Explants from 7, 8, 9, 11, 13-day chick embryonic skin incorporating (3H) Uridine for different periods 1 hr, 3 or 4 hr and a chase with actinomycin) are studied with respect to free (F) or membrane bound (B) cytoplasmic polysomes and to RNA extracted from them. Polysome specific activity decreases at older stages but...

journal_title：Biochimie

authors： Desveaux-Chabrol J

更新日期：1976-01-01 00:00:00

abstract：:Tryptocidine C (TpcC, cyclo[D-Phe1-Pro2-Trp3-D-Trp4-Asn5-Gln6-Trp7-Val8-Orn9-Leu10]) is a broad-spectrum antimicrobial peptide in the tyrothricin complex produced by a soil bacterium, Brevibacillus parabrevis. Electrospray mass spectrometric studies reveal the oligomerisation of TpcC into dimers and higher oligomers, ...

journal_title：Biochimie

authors： Rautenbach M,Kumar V,Vosloo JA,Masoudi Y,van Wyk RJ,Stander MA

更新日期：2020-12-18 00:00:00

abstract：:Theoretic and experimental arguments are surveyed which justify the setting up, within the family of pyridoxal-P-dependent lyases, of a special subgroup that comprizes several enzymes catalyzing exclusively beta-replacement reactions of alpha-aminoacids with electronegative substituents in the beta-position. The autho...

journal_title：Biochimie

authors： Braunstein AE,Goryachenkova EV

更新日期：1976-01-01 00:00:00

abstract：:All phytopathogenic fungi have two catalase-peroxidase paralogues located either intracellularly (KatG1) or extracellularly (KatG2). Here, for the first time a secreted bifunctional, homodimeric catalase-peroxidase (KatG2 from the rice blast fungus Magnaporthe grisea) has been produced heterologously with almost 100% ...

journal_title：Biochimie

authors： Zámocký M,Droghetti E,Bellei M,Gasselhuber B,Pabst M,Furtmüller PG,Battistuzzi G,Smulevich G,Obinger C

更新日期：2012-03-01 00:00:00

abstract：:Evidence shows that oral glycerol supplementation during the early stages of rat liver cancer reduces the growth of preneoplastic lesions. Besides, human hepatocellular carcinoma (HCC) cells display decreased expression of glycerol channel aquaporin 9 (AQP9) and also diminished glycerol-3-phosphate (G3P) content. Acco...

journal_title：Biochimie

authors： Lorenzetti F,Capiglioni AM,Marinelli RA,Carrillo MC,Alvarez ML

更新日期：2020-03-01 00:00:00

abstract：:Pseudomonas cepacia 122 can grow on several lignin model compounds including the arylglycerol-beta-aryl ethers guaiacylglycerol-beta-coniferyl ether and guaiacylglycerol-beta-guaiacyl ether. Non-phenolic lignin model compounds are not degraded by this bacterium. The enzyme system catalyzing guaiacylglycerol-beta-guaia...

journal_title：Biochimie

authors： Odier E,Rolando C

更新日期：1985-02-01 00:00:00

abstract：:Nitroxyl (HNO) is a molecule of significant interest due to its unique pharmacological properties, particularly within the cardiovascular system. A large portion of HNO biological effects can be attributed to its reactivity with protein thiols, where it can generate disulfide bonds. Evidence from studies in erythrocyt...

journal_title：Biochimie

authors： Salie MJ,Oram DS,Kuipers DP,Scripture JP,Chenge J,MacDonald GJ,Louters LL

更新日期：2012-03-01 00:00:00

abstract：:A variety of transcription attenuation mechanisms are used by bacteria to regulate gene and operon expression. This review summarizes previous and current studies designed to elucidate the features of the specific attenuation mechanisms that regulate expression of the tryptophanase (tna) operon of Escherichia coli and...

journal_title：Biochimie

authors： Yanofsky C,Konan KV,Sarsero JP

更新日期：1996-01-01 00:00:00

abstract：:Three trends are seen in the organization of ribosomal DNA genes during evolution: 1) gradual separation and separability of the regulation of transcription of 5S and larger subunit rRNAs; 2) retention of a transcription unit containing both large and small rRNAs; and 3) clustering of genes for both 5S and 18S-28S rDN...

journal_title：Biochimie

authors： Srivastava AK,Schlessinger D

更新日期：1991-06-01 00:00:00

abstract：:Microalgae are single-cell, photosynthetic organisms whose biodiversity places them at the forefront of biological producers of high-value molecules including lipids and pigments. Some of these organisms particular are capable of synthesizing n-3 very long chain polyunsaturated fatty acids (VLC-PUFAs), known to have b...

journal_title：Biochimie

authors： Lupette J,Benning C

更新日期：2020-11-01 00:00:00

abstract：:The genus Populus is an effective model in tree genetics. This genus includes dioecious species and, recently, whole genome resequencing of P. trichocarpa and P. balsamifera enabled the identification of sex-linked regions and sex-associated single nucleotide polymorphisms (SNPs). These results created new opportuniti...

journal_title：Biochimie

authors： Melnikova NV,Kudryavtseva AV,Borkhert EV,Pushkova EN,Fedorova MS,Snezhkina AV,Krasnov GS,Dmitriev AA

更新日期：2019-07-01 00:00:00

abstract：:Structural and motional features in the denatured state of a protein dictate the early folding events starting from that state and these features vary depending upon the nature of the denaturant used. Here, we have attempted to decipher the early events in the folding of Dynein Light Chain protein (DLC8), starting fro...

journal_title：Biochimie

authors： Chakraborty S,Mohan PM,Hosur RV

更新日期：2012-01-01 00:00:00

abstract：:Three different hydrogenases (isolated from Clostridium pasteurianum, Desulfovibrio desulfuricans strain Norway 4 and D. baculatus 9974) added to a suspension of TiO2 (anatase) powder are able to catalyze H2 evolution under band gap illumination of the semiconducting particles, and in the presence of EDTA or methanol ...

journal_title：Biochimie

authors： Cuendet P,Rao KK,Grätzel M,Hall DO

更新日期：1986-01-01 00:00:00

abstract：:The nucleotide sequence of the gene encoding chicken ribosomal protein L7a was determined. The gene contains eight exons and seven introns, which spread over 3613 nucleotides. The transcription initiation sites were located on four consecutive nucleotides GCCC, which are at the 5' terminus of a short polypyrimidine tr...

journal_title：Biochimie

authors： Maeda N,Kenmochi N,Tanaka T

更新日期：1993-01-01 00:00:00

abstract：:Kinetic properties of PGM1 and PGM2 phosphoglucomutase "primary" isoenzymes from human erythrocytes were studied. The two enzyme forms share a "ping-pong" kinetic mechanism and show similar Km for substrate (glucose 1-P) and cofactor (glucose 1,6-P2). Micromolar concentrations of fructose 1,6-P2 and glycerate 2,3-P2 i...

journal_title：Biochimie

authors： Ninfali P,Accorsi A,Palma F,Fazi A,Piatti E,Chiarantini L,Fornaini G

更新日期：1984-09-01 00:00:00

abstract：:Reuber H 35 hepatoma cells were synchronized by transfer in a serum free medium. Growth was re-initiated by addition of serum. Under these conditions DNA synthesis exhibited a maximum after 24 hours. Chromatin non-histone proteins prepared from cells at various phases of the cell cycle were incubated with [gamma-32P] ...

journal_title：Biochimie

authors： Defer N,Tichonicky L,Kruh J,Zoutewelle G,Van Wijk R

更新日期：1979-01-01 00:00:00

abstract：:In order to identify conserved potential secondary structures within translational start sites, mRNA sequences derived from different species were studied with programs able to depict such features. The potential secondary structure of 71 bases around the initiator AUG or AUGs in the coding sequences of 290 eukaryotic...

journal_title：Biochimie

authors： Ganoza MC,Louis BG

更新日期：1994-01-01 00:00:00

abstract：:Labeling of 21-kDa material was observed when bovine brain soluble fraction was incubated with [adenylate-32P]NAD+ in the presence of GTP. The 21-kDa substrate, slightly smaller than C3 substrate in size, was labeled even without C3 exoenzyme. GTP could be replaced by nucleoside triphosphates other than ATP while ATP ...

journal_title：Biochimie

authors： Badruzzaman M,Miyaoka T,Terashima M,Yamada K,Ohno T,Yamamori C,Tsuchiya M,Shimoyama M

更新日期：1997-07-01 00:00:00

abstract：:The effects of thermal injury (72 h post-injury), 72 h-partial (20% less food) or full starvation on the regulation of phosphofructokinase in the mucosa of rat small intestine were studied. Thermal injury and 72 h-partial or full starvation decreased the activity ratio v0.5/V, but the ratios obtained for thermally inj...

journal_title：Biochimie

authors： Khoja SM,Ardawi SM

更新日期：1987-01-01 00:00:00

abstract：:EPR spectroscopy in conjunction with oxidation-reduction potentiometry has been used to determine the half-reduction potentials of the four hemes of cytochrome c3. As predicted, the four hemes of cytochrome c3 have different mid-point potentials. The Em values are: Heme I,--284 mV; Heme II,--310 mV; Heme III,--324 mV ...

journal_title：Biochimie

authors： Dervartanian DV,Xavier AV,Gall JL

更新日期：1978-01-01 00:00:00

abstract：:gamma-Aminobutyraldehyde dehydrogenase from Escherichia coli K-12 has been purified and characterized from cell mutants able to grow in putrescine as the sole carbon and nitrogen source. The enzyme has an Mr of 195,000 +/- 10,000 in its dimeric form with an Mr of 95,000 +/- 1,000 for each subunit, a pH optimum at 5.4 ...

journal_title：Biochimie

authors： Prieto MI,Martin J,Balaña-Fouce R,Garrido-Pertierra A

更新日期：1987-11-01 00:00:00

abstract：:The human liver fatty acid binding protein (hFABP1) participates in cellular long-chain fatty acid trafficking and regulation of lipid metabolism and changes in hFABP1 are associated with an increased risk for type 2 diabetes, cardiovascular disease (CVD), and metabolic syndromes. Gene regulation of hFABP1 is not full...

journal_title：Biochimie

authors： Wu YL,Peng XE,Wang D,Chen WN,Lin X

更新日期：2012-02-01 00:00:00

abstract：:Bacterial superantigens (SAg) are the most potent activators of human T lymphocytes and recombinant immunotoxin using bacterial SAg shows promising clinical values. To engineer superantigen for immunotherapy of hepatocellular carcinoma, we genetically fused the superantigen staphylococcus enterotoxin A (SEA(D(227)A)) ...

journal_title：Biochimie

authors： Hao HJ,Jiang YQ,Zheng YL,Ma R,Yu DW

更新日期：2005-08-01 00:00:00

abstract：:Microsomal membranes from potato tubers were extracted by acetone solutions of increasing concentrations (5, 10, 15, 20, 30, 40, 50, 70 and 90 p. cent). Microsomal lipids were progressively extracted: acetone concentrations exceeding 30 p. cent extracted large amounts of membraneous phospholipids (figure). Lipid extra...

journal_title：Biochimie

authors： Jolliot A,Demandre C,Mazliak P

更新日期：1978-01-01 00:00:00

abstract：:In vitro cycling rates of E. coli ribosomes and of elongation factors EF-Tu and EF-G have been obtained and these are compatible with translation rates in vivo. We show that the rate of translocation is faster than 50 s-1 and therefore that the EF-G function is not a rate limiting step in protein synthesis. The in viv...

journal_title：Biochimie

authors： Bilgin N,Kirsebom LA,Ehrenberg M,Kurland CG

更新日期：1988-05-01 00:00:00

abstract：:We isolated and purified the main lipids of the rat sciatic nerve. After methanolysis, fatty acids were isolated and purified by thin layer chromatography, and studied by gas chromatography. C 16 and C 18 fatty acids are the most abundant. Among the long-chain fatty acids, arachidonic acid (20:4) is present in some li...

journal_title：Biochimie

authors： Klein F,Mandel P

更新日期：1978-01-01 00:00:00

abstract：:The hbs operon of Bacillus subtilis comprises a single gene which is localized between the spoIVA and mtrA open reading frames, and is situated at 204 degrees of the standard map of B subtilis. Expression of hbs is initiated from two distinct promoters called P1 and P2. The transcription initiation sites have been map...

journal_title：Biochimie

authors： Micka B,Marahiel MA

更新日期：1992-07-01 00:00:00

abstract：:Mesenchymal stem cells (MSC) show great promise in a wide array of therapeutic applications due mainly to their capacity to suppress immune and inflammatory reactions and instigate normal tissue repair processes. The secretion of bioactive factors is thought to play a predominant role in the mechanisms of action for t...

journal_title：Biochimie

authors： Lavoie JR,Rosu-Myles M

更新日期：2013-12-01 00:00:00