Abstract：
:The maximum activities of some key enzymes of metabolism were studied in lungs of fed and 48-h-starved rats. The maximum activity of hexokinase in the lung is similar to that of other tissues of the body, but lower than that of phosphorylase and 6-phosphofructokinase. High activities of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase were found in lung tissue, suggesting the importance of the pentose phosphate pathway in the lung. The activities of hexokinase and 6-phosphofructokinase were decreased whereas that of phosphorylase increased in response to starvation. Of the enzymes of the tricarboxylic acid cycle whose activities were measured, that of oxoglutarate dehydrogenase was the lowest, yet its activity (approximately 4.2 nmol/min per mg protein at 37 degrees C) was considerably greater than the flux through the cycle (0.46 nmol/min per mg protein at 37 degrees C; calculated from oxygen consumption by incubated lung slices). The activities of both oxoglutarate dehydrogenase and citrate synthase were decreased by starvation. The activities of 3-oxoacid CoA-transferase and acetoacetyl-CoA thiolase were low in lung tissue compared to those of other tissues (eg kidney, brain) and that of 3-hydroxybutyrate dehydrogenase was very low. The activity of carnitine palmitoyl transferase is higher in the lung, suggesting that fatty acids (and possibly acetoacetate) could provide acetyl-CoA as substrate for the tricarboxylic acid cycle. Very low rates of utilization of 3-hydroxybutyrate were observed during incubation of lung slices, but that of oleate was 1.2 nmol/h per mg of protein.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochimiejournal_title
Biochimieauthors
Ardawi MSdoi
10.1016/0300-9084(91)90023-tsubject
Has Abstractpub_date
1991-05-01 00:00:00pages
557-62issue
5eissn
0300-9084issn
1638-6183pii
0300-9084(91)90023-Tjournal_volume
73pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:Kinetic parameters characterizing the catalytic activities of enzymes are typically investigated in dilute solutions. However, in reality, these reactions occur in cells that, in addition to water and ions, are full of other macromolecules including proteins, nucleic acids, lipids, and metabolites. Such a crowded envi...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.07.009
更新日期：2020-09-01 00:00:00
abstract：:The use of electrospun scaffolds for neural tissue engineering applications allows a closer mimicry of the native tissue extracellular matrix (ECM), important for the transplantation of cells in vivo. Moreover, the role of the electrospun fiber mat topography on neural stem cell (NSC) differentiation remains to be com...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2021.01.001
更新日期：2021-01-07 00:00:00
abstract：:The effect of cycloheximide on rRNA and poly (A) (+) RNA synthesis is studied in radish seedlings. When used at a concentration of 50 microng/ml cycloheximide selectively blocks rRNA synthesis without altering poly (A) (+) RNA synthesis. Processing of the 2.3 X 10(6) dalton pre-rRNA is severely impaired. This observat...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(77)80085-0
更新日期：1977-01-01 00:00:00
abstract：:The hypothesis is proposed that pre-biotic bacterial cell(s) and the first cells capable of growth/division did not require a cytoplasmic membrane. A gel-like microscopic structure less than a cubic micrometer may have had a dual role as both an ancient pre-cytoplasm and a boundary layer to the higher-entropy external...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.10.002
更新日期：2012-01-01 00:00:00
abstract：:Porcine thyroid cells in primary cultures form either monolayers or, when they are stimulated by thyrotropin (TSH), follicles. This system, monolayer-follicle associated cell culture, determines two morphofunctional states of the thyroid cell in vitro. These cells divide, when grown in monolayer. In this article we de...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(80)80367-1
更新日期：1980-01-01 00:00:00
abstract：:Hemorrhage is the most potent manifestation of envenomation by Vipera ammodytes ammodytes (V. a. ammodytes) venom in man. A detailed description of the venom components contributing to this effect is thus medically very important. We have characterized a novel component, termed here VaH3, as a potently hemorrhagic sna...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.01.003
更新日期：2013-06-01 00:00:00
abstract：:Current research in biology heavily depends on the availability and efficient use of information. In order to build new knowledge, various sources of biological data must often be combined. Semantic Web technologies, which provide a common framework allowing data to be shared and reused between applications, can be ap...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2008.02.007
更新日期：2008-04-01 00:00:00
abstract：:The central glycolytic and oxidative pathways and the ATP-producing mechanisms differ in sane and malignant cells by their regulation and dynamics. Fast-growing, poorly-differentiated cancer cells characteristically show high aerobic glycolysis. In the same way, cholesterol biosynthesis, which occurs by normal pathway...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(92)90016-8
更新日期：1992-11-01 00:00:00
abstract：:Oligonucleotides that complement Escherichia coli 16S ribosomal RNA residues 685-696 and 694-705 have been synthesized so as to incorporate antibody-recognizable markers: a 3'-terminal residue of N6-delta 2-isopentenyladenosine, a 5'-dinitrophenyl group, or both. Each oligonucleotide is able to bind RNA within the sma...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(91)90132-k
更新日期：1991-07-01 00:00:00
abstract：:RNA domain swapping typically demonstrates conservation of the native function of the domain in a non-native context. In contrast, we employed RNA engineering to demonstrate deviation of G-quadruplex (GQ) function that is contingent upon its context dependent location, which is opposite to their native functional role...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.03.007
更新日期：2017-06-01 00:00:00
abstract：:The chemical carcinogen N-acetoxy-N-2-acetylaminofluorene (N-AcO-AAF) induces frameshift mutations located within two types of specific sequences (mutation hot spots): i) contiguous guanine sequences and ii) alternating GC sequences. The genetic requirements of these frameshift events were investigated using specific ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(91)90117-j
更新日期：1991-04-01 00:00:00
abstract：:As the medaka is a popular fish model in genetics, developmental biology and toxicology, the development of an efficient transgenic medaka technique is important for a variety of biological experiments. Here we demonstrated that the maize transposon system, Ac/Ds, greatly improved the transgenesis of microinjected DNA...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.07.006
更新日期：2011-10-01 00:00:00
abstract：:The phosphotriesterase PTE, identified in the soil bacterium Pseudomonas diminuta, is thought to have evolved in the last several decades to degrade the pesticide paraoxon with proficiency approaching the limit of substrate diffusion (k(cat)/K(M) of 4 x 10(7)M(-1)s(-1)). It belongs to the amidohydrolase superfamily, b...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2007.01.007
更新日期：2007-05-01 00:00:00
abstract：:A d-carbamoylase from Sinorhizobium morelens S-5 was purified and characterized. The enzyme was purified 189-fold to homogeneity with a yield of 19.1% by aqueous two-phase extraction and two steps of column chromatography. The enzyme is a homotetramer with a native molecular mass of 150 kDa and a subunit relative mole...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2005.05.013
更新日期：2006-03-01 00:00:00
abstract：:Peptidase family S46 consists of two types of dipeptidyl-peptidases (DPPs), DPP7 and DPP11, which liberate dipeptides from the N-termini of polypeptides along with the penultimate hydrophobic and acidic residues, respectively. Their specificities are primarily defined by a single amino acid residue, Gly673 in DPP7 and...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.10.015
更新日期：2018-04-01 00:00:00
abstract：:Stereospecific hydrogenations according to the general scheme [formula: see text] are of interest from a preparative and mechanistic point of view. Proteus mirabilis is suitable for the hydrogenation of a-keto-acids to R-hydroxy-acids, and a Clostridium strain for the reduction of enoates. Both have been immobilized i...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(80)80162-3
更新日期：1980-01-01 00:00:00
abstract：:Since discovery of high-temperature superconductive (HTS) ceramics by Bednorz and Muller in 1986, there has been an accelerated development of cold technologies in industry, including the domain of NMR detection. The purpose of this paper is to fix ideas about the stage that cryogenic radio frequency (RF) probe techni...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2003.09.016
更新日期：2003-09-01 00:00:00
abstract：:Clustered apurinic/apyrimidinic (AP) sites are more cytotoxic than isolated AP lesions because double strand breaks (DSB) can be formed during repair of closely positioned bistranded AP sites. Formation of DSB due to simultaneous cleavage of bistranded AP sites may be regulated by proteins specifically interacting wit...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2018.04.027
更新日期：2018-07-01 00:00:00
abstract：:The kinetic and regulatory properties of purified pigeon heart muscle AMP deaminase were investigated. In the presence of 100 mM potassium chloride, the enzyme exhibited a slightly sigmoidal type of kinetics. Addition of ATP to the incubation medium changed the reaction rate versus substrate concentration plot into a ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(87)90243-4
更新日期：1987-02-01 00:00:00
abstract：:Mouse brain myelin consists of a continuum of particles of different densities, as shown by sucrose density gradient centrifugation. In normal animals most of the material (65 per cent) is concentrated between 0.6 and 0.7 M sucrose (the maximum being found at 0.66 M sucrose, corresponding to 23 per cent). The density ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(77)80213-7
更新日期：1977-01-01 00:00:00
abstract：:Sticholysin II (StII) is a pore-forming toxin of biomedical interest that belongs to the actinoporin protein family. Sticholysins are currently under examination as an active immunomodulating component of a vaccinal platform against tumoral cells and as a key element of a nucleic acids delivery system to cell cytosol....
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2018.02.006
更新日期：2018-05-01 00:00:00
abstract：:Poly(ADP-ribosyl)ation is a posttranslational protein modification significant for genomic stability and cell survival in response to DNA damage. Poly(ADP-ribosyl)ation is catalyzed by poly(ADP-ribose)polymerases (PARPs). Among the 17 members of the PARP family, PARP-1 and PARP-2 are described as enzymes whose catalyt...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.01.007
更新日期：2013-06-01 00:00:00
abstract：:The preadipocyte cell lines 3T3-L1 and 3T3-F442A are widely used to study the cellular mechanisms of preadipocyte differentiation and mature adipocyte functions. However, transfection with naked DNA is inefficient in these cell lines. Adenoviral gene transfer is a powerful technique to induce high levels of transgene ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2005.05.010
更新日期：2005-11-01 00:00:00
abstract：:Bacillus subtilis strain SO113 secretes a pectate lyase which is produced during the exponential death phase of growth, just before sporulation. This extracellular pectate lyase, which produces unsaturated products from polygalacturonate, was purified 35-fold from the culture supernatant of Bacillus subtilis by a CM S...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(90)90053-j
更新日期：1990-09-01 00:00:00
abstract：:The secretome produced by cells within the bone marrow is significant to homeostasis. The bone marrow, a well-studied organ, has multiple niches with distinct roles for supporting stem cell functions. Thus, an understanding of mediators involved in the regulation of stem cells could serve as a model for clinical probl...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2018.05.018
更新日期：2018-12-01 00:00:00
abstract：:Interferon-gamma is a key cytokine in tumor immunosurveillance. The recently described interferon-producing killer dendritic cell (IKDC), can be distinguished from other innate effectors by its ability to kill a large variety of tumor cells and to produce high amounts of interferon-gamma after encountering tumors in t...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2007.04.014
更新日期：2007-06-01 00:00:00
abstract：:The proton NMR data of a synthetic model of deoxy-myoglobin and hemoglobin show one that:(i) the ground state of the high spin iron (II) complex must be described by at least two electronic levels separated by ca.200 cm-1 and resulting from the removal of the degeneracy of the 5E level; (ii) this in-plane rhombic dist...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(82)80285-x
更新日期：1981-11-01 00:00:00
abstract：:The dissociation of bound polyribosomes from ewe lactating mammary gland, by EDTA or Puromycin-KC1 treatment releases Messenger Ribonucleoprotein particles (mRNP'S) which sediment in sucrose gradients as a single component having a sedimentation coefficient of about 22S. RNAs derived from mRNPs sediment between 9S and...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(76)80439-7
更新日期：1976-01-01 00:00:00
abstract：:Fluorometry using a substrate DNA labeled with a single fluorophore (6-carboxyfluorescein) at the 3'-end of the processed strand was shown to be a useful tool for monitoring DNA-binding and 3'-processing activities of HIV-1 and PFV integrases (INs). The DNA binding to either of the INs resulted in a fluorescence signa...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2012.06.009
更新日期：2012-11-01 00:00:00
abstract：:Although halogenated hydrocarbons are noted for low chemical reactivity, small amounts are toxic to humans. Cytochromes P450 have been implicated in transforming these compounds to more reactive species, under anaerobic conditions, through reduction at the heme. A significant amount of effort has been directed toward ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(97)82529-1
更新日期：1996-01-01 00:00:00
