Abstract：
:RNA domain swapping typically demonstrates conservation of the native function of the domain in a non-native context. In contrast, we employed RNA engineering to demonstrate deviation of G-quadruplex (GQ) function that is contingent upon its context dependent location, which is opposite to their native functional role. Known translation repressing RNA GQs were engineered into human VEGF IRES A replacing the endogenous GQ domain essential for translation. Alternatively, the translation inhibitory GQ motif within the 5'-UTR of MT3-MMP mRNA was replaced with two known GQ motifs that are essential for translation. The results indicate that the engineered GQ domains can adopt GQ structures in a foreign environment with a functional role reversal to accommodate the need of the endogenous swapped motifs. The observations establish the functionality and context dependent modularity of RNA GQ structures.
journal_name
Biochimiejournal_title
Biochimieauthors
Bhattacharyya D,Morris MJ,Kharel P,Mirihana Arachchilage G,Fedeli KM,Basu Sdoi
10.1016/j.biochi.2017.03.007subject
Has Abstractpub_date
2017-06-01 00:00:00pages
147-150eissn
0300-9084issn
1638-6183pii
S0300-9084(17)30060-3journal_volume
137pub_type
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