Several classes of binding sites for metals and nucleotides on yeast mitochondrial oligomycin sensitive ATPase.

abstract：:The adaptive response appears in E. coli after exposure to low levels of alkylating agents. This system is under the positive control of the ada gene. At least two enzymes are induced during the response: 3-methyladenine DNA glycosylase II and O6-methylguanine DNA methyltransferase. The latter is also the product of t...

journal_title：Biochimie

authors： Defais M

更新日期：1985-03-01 00:00:00

abstract：:Colorectal cancer (CRC) is the third most diagnosed cancer in the western world, affecting 1 out of approximately 22 people in their lifetime. Several epidemiological studies suggest a positive association between high plasma cholesterol levels and colorectal cancer. However, the molecular mechanisms by which choleste...

journal_title：Biochimie

authors： Warns J,Marwarha G,Freking N,Ghribi O

更新日期：2018-10-01 00:00:00

abstract：:A series of halomethylated derivatives of dihydrocoumarins has been found to inhibit irreversibly proteases and esterases. alpha-Chymotrypsin, subtilish, elastase are rapidly inactivated in the presence of these compounds, while trypsin, kallicrein, papain are inhibited more slowly. Esterases like acetylcholinesterase...

journal_title：Biochimie

authors： Béchet JJ,Dupaix A,Roucous C,Bonamy AM

更新日期：1977-01-01 00:00:00

abstract：:The purpose of the present study was to investigate the effect of a concentrated preparation (EPA 30) containing eicosapentaenoic acid (EPA, 20:5 n-3) and docosahexaenoic acid (DHA, 22:6 n-3) on the limiting desaturation steps of the polyunsaturated fatty acid biosynthesis in spontaneously hypertensive rats (SHR). Adu...

journal_title：Biochimie

authors： Narce M,Frenoux JM,Dardel V,Foucher C,Germain S,Delachambre MC,Poisson JP

更新日期：1997-02-01 00:00:00

abstract：:Glutaredoxins (Grxs), redox-active proteins with a typical -CPYC motif at their active sites, are involved in redox-regulatory processes and antioxidant defenses. The human malarial parasite Plasmodium falciparum possess a classical glutaredoxin (PfGrx) as well as a number of Grx-like proteins. In the present study, w...

journal_title：Biochimie

authors： Tripathi T,Röseler A,Rahlfs S,Becker K,Bhakuni V

更新日期：2010-03-01 00:00:00

abstract：:The synthesis of S100 protein increases toward the end of the exponential phase of growth of clonal rat glial cells C6 in monolayer culture. Moreover the synthesis of this protein can be increased by treatment of C6 cells with the lectin succinylated concanavalin A (succinyl ConA). In order to study the relationship b...

journal_title：Biochimie

authors： Marks A,Law J,Mahony J

更新日期：1983-11-01 00:00:00

abstract：:Epidemiological studies have demonstrated that the diabetes mellitus is a serious health burden for both governments and healthcare providers. The present study was hypothesized to evaluate the antihyperglycemic potential of fraxetin by determining the activities of key enzymes of carbohydrate metabolism in streptozot...

journal_title：Biochimie

authors： Murali R,Srinivasan S,Ashokkumar N

更新日期：2013-10-01 00:00:00

abstract：:Carbonic anhydrases (CAs, EC 4.2.1.1) are metalloenzymes found throughout the phylogenetic tree. The β-class carbonic anhydrases (β-CAs) are the predominating class of CAs in plants. Growing evidence underscores the importance of β-CAs in plant immunity and environmental adaptation in addition to their roles in photos...

journal_title：Biochimie

authors： Wang M,Zhang Q,Liu FC,Xie WF,Wang GD,Wang J,Gao QH,Duan K

更新日期：2014-02-01 00:00:00

abstract：:Oxidative stress, characterized by the accumulation of reactive oxygen species (ROS), is known to have numerous detrimental effects on the myocardium such as the induction of apoptotic cell death, hypertrophy, fibrosis, dysfunction, and dilatation. Over the past several years, we have shown that mitochondrial NADP(+)-...

journal_title：Biochimie

authors： Lee JH,Park JW

更新日期：2014-04-01 00:00:00

abstract：BACKGROUND:The modest efficacy of available therapies for Hepatocellular carcinoma (HCC) indicates the need to develop novel therapeutic approaches. For the proteasome inhibitor Bortezomib (BZB), potentially attractive for HCC treatment, the mechanism of action is largely unknown. The BZB effect on E2Fs and the E2Fs co...

journal_title：Biochimie

authors： Farra R,Dapas B,Baiz D,Tonon F,Chiaretti S,Del Sal G,Rustighi A,Elvassore N,Pozzato G,Grassi M,Grassi G

更新日期：2015-05-01 00:00:00

abstract：:We have produced and purified an active site mutant of the Escherichia coli cyclopropane fatty acid synthase (CFAS) by replacing the strictly conserved G236 within cyclopropane synthases, by a glutamate residue, which corresponds to E146 of the homologous mycolic acid methyltransferase, Hma, producing hydroxymethyl my...

journal_title：Biochimie

authors： E G,Drujon T,Correia I,Ploux O,Guianvarc'h D

更新日期：2013-12-01 00:00:00

abstract：:Surfactants carrying either a hydrocarbon or a fluorocarbon alkyl chain have been synthesized. The polar head was either tris(hydroxymethyl)acrylamidomethane (THAM), telomerized THAM, or a glycosylated THAM moiety. The aqueous solubility of some of these molecules was increased by oxidizing to a sulfoxide the thioethe...

journal_title：Biochimie

authors： Chabaud E,Barthélémy P,Mora N,Popot JL,Pucci B

更新日期：1998-05-01 00:00:00

abstract：:The use of electrospun scaffolds for neural tissue engineering applications allows a closer mimicry of the native tissue extracellular matrix (ECM), important for the transplantation of cells in vivo. Moreover, the role of the electrospun fiber mat topography on neural stem cell (NSC) differentiation remains to be com...

journal_title：Biochimie

authors： Garrudo FFF,Mikael PE,Xia K,Silva JC,Ouyang Y,Chapman CA,Hoffman PR,Yu Y,Han X,Rodrigues CAV,Cabral JMS,Morgado J,Ferreira FC,Linhardt RJ

更新日期：2021-01-07 00:00:00

abstract：:We screened a cDNA library of the primitive eukaryote Dictyostelium discoideum constructed in the expression vector lambda gt11 with a specific antiserum directed against S-adenosyl-L-homocysteine hydrolase (AdoHcy hydrolase) and isolated cDNA clones coding for fusion proteins with beta-galactosidase. The identity of ...

journal_title：Biochimie

authors： Guitton MC,Part D,Veron M

更新日期：1988-06-01 00:00:00

abstract：:Leishmania is a unicellular protozoan parasite which causes leishmaniasis, a neglected tropical disease. It possess a unique thiol metabolism comprising of several proteins among which, tryparedoxin (cTXN) and tryparedoxin peroxidase (cTXNPx), function in concert as oxidoreductases, utilizing trypanothione as a source...

journal_title：Biochimie

authors： Suman SS,Equbal A,Zaidi A,Ansari MY,Singh KP,Singh K,Purkait B,Sahoo GC,Bimal S,Das P,Ali V

更新日期：2016-02-01 00:00:00

abstract：:Total poly (A+) RNA has been extracted from rat pheochromocytoma and translated in vitro by means of a reticulocyte lysate system. We show that two antisera, prepared against pig kidney DOPA decarboxylase (DDC) or rat pheochromocytoma DDC, immunoprecipitate an in vitro synthetized 50 kDa polypeptide identified as DDC ...

journal_title：Biochimie

authors： Bruneau G,Krieger-Poullet M,Coge F,Borri-Voltattorni C,Gros F,Thibault J

更新日期：1990-01-01 00:00:00

abstract：:A new apolipoprotein has been identified in VHDL1 and in HDL. This protein is immunologically distinct from already isolated apoproteins. It was isolated by column chromatography on hydroxylapatite. In polyacrylamide gel electrophoresis, its mobility is very close to that of apo D. The amino acid composition differs f...

journal_title：Biochimie

authors： Ayrault-Jarrier M,Alix JF,Polonovski J

更新日期：1978-01-01 00:00:00

abstract：:The cholesterol lowering drug lovastatin, a competitive inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, blocks DNA synthesis and proliferation of thyrotropin (TSH) primed FRTL-5 rat thyroid cells. The blockade can be completely prevented and/or reversed by mevalonate and largely prevented and/o...

journal_title：Biochimie

authors： Bifulco M,Laezza C,Aloj SM

更新日期：1999-04-01 00:00:00

abstract：:Long-term exposure to high doses of caffeine affects several aspects of nitrogen metabolism, such as purine, pyrimidine and urea synthesis. However, little is known about the mechanisms of these changes and if they occur at shorter term. We have studied in isolated hepatocytes: 1) the in vitro effect of high doses of ...

journal_title：Biochimie

authors： Jordá A,Saéz GT,Portolés M,Pallardó FV,Jimenez-Nacher I,Gascoó E

更新日期：1988-10-01 00:00:00

abstract：:The preadipocyte cell lines 3T3-L1 and 3T3-F442A are widely used to study the cellular mechanisms of preadipocyte differentiation and mature adipocyte functions. However, transfection with naked DNA is inefficient in these cell lines. Adenoviral gene transfer is a powerful technique to induce high levels of transgene ...

journal_title：Biochimie

authors： Béréziat V,Moritz S,Klonjkowski B,Decaudain A,Auclair M,Eloit M,Capeau J,Vigouroux C

更新日期：2005-11-01 00:00:00

abstract：:GcvB is a non-coding RNA that regulates oppA mRNA in different bacterial species by binding a GcvB GU-rich region named R1 to oppA mRNA. A secondary putative interaction site (PS1) was identified in this study that is able to form a second nearly perfect 10 base-pair duplex between these two RNAs in Escherichia coli. ...

journal_title：Biochimie

authors： Busi F,Le Derout J,Cerciat M,Régnier P,Hajnsdorf E

更新日期：2010-10-01 00:00:00

abstract：:The very end of the chromosome is called the telomere and is composed of DNA repeat sequences and associated proteins. Genetic and biochemical analyses of this complex, the telosome, lead to the hypothesis that transcription and DNA replication are submitted to position effects mediated by the telomere proximity. Telo...

journal_title：Biochimie

authors： Pommier JP,Lebeau J,Ducray C,Sabatier L

更新日期：1995-01-01 00:00:00

abstract：:The conformation of perch parvalbumin in the Ca-, Mg- and metal-free state was studied by intrinsic fluorescence, trypsin susceptibility, thiol titration and circular dichroism. The data reveal that Ca-parvalbumin has a more compact structure than the metal-free protein, with a high alpha-helical content and a buried ...

journal_title：Biochimie

authors： Cox JA,Winge DR,Stein EA

更新日期：1979-01-01 00:00:00

abstract：:Mitochondrial function requires genes encoded in both mitochondrial and nuclear genomes. Tfam, the activator of mammalian mitochondrial transcription, is encoded in the nucleus and its expression has been shown in in vitro studies to be controlled by nuclear respiratory factors NRF-1 and NRF-2. In order to understand ...

journal_title：Biochimie

authors： Escrivá H,Rodríguez-Peña A,Vallejo CG

更新日期：1999-10-01 00:00:00

abstract：:Sphingolipids are degraded by sphingomyelinase and ceramidase in the gut to ceramide and sphingosine, which may inhibit cell proliferation and induce apoptosis, and thus have anti-tumour effects in the gut. Although previous rodent studies including experiments on knockout mice indicate a role of neutral ceramidase in...

journal_title：Biochimie

authors： Ohlsson L,Palmberg C,Duan RD,Olsson M,Bergman T,Nilsson A

更新日期：2007-08-01 00:00:00

abstract：:Current research in biology heavily depends on the availability and efficient use of information. In order to build new knowledge, various sources of biological data must often be combined. Semantic Web technologies, which provide a common framework allowing data to be shared and reused between applications, can be ap...

journal_title：Biochimie

authors： Pasquier C

更新日期：2008-04-01 00:00:00

abstract：:The Gag-encoded nucleocapsid protein NCp7 (72 amino acids) from HIV-1, the regulatory protein, Vpr (96 amino acids) and numerous derivatives have been synthesized by solid phase method and their structures determined by 2D NMR. In NCp7, the two highly folded zinc fingers of the Cx2Cx4Hx4C type are in close spacial pro...

journal_title：Biochimie

authors： Roques BP,Morellet N,de Rocquigny H,Déméné H,Schueler W,Jullian N

更新日期：1997-11-01 00:00:00

abstract：:High-mobility group box-1 (HMGB1) is remarkably mobile in living cells, which reflects its ability to interact only transiently with both DNA and protein. This property is likely essential for HMGB1 nuclear activities. Nonetheless the weak interaction of HMGB1 with DNA and/or protein partners has also been a major lim...

journal_title：Biochimie

authors： Messmer M,Klein C,Boniface R,Gnädig NF,Lecerf M,Barnay-Verdier S,Maréchal V

更新日期：2013-04-01 00:00:00

abstract：:Developing a logical and rational methodology for obtaining vaccines, especially against the main parasite causing human malaria (P. falciparum), consists of blocking receptor-ligand interactions. Conserved peptides derived from proteins involved in invasion and having high red blood cell binding ability have thus bee...

journal_title：Biochimie

authors： Patarroyo ME,Salazar LM,Cifuentes G,Lozano JM,Delgado G,Rivera Z,Rosas J,Vargas LE

更新日期：2006-02-01 00:00:00

abstract：:Here we describe a convenient and robust ligase-independent method for construction of combinatorial and random mutant libraries. The homologous genes flanked by plasmid-derived DNA sequences are fragmented, and the random fragments are reassembled in a self-priming polymerase reaction to obtain chimeric genes. The pr...

journal_title：Biochimie

authors： An Y,Wu W,Lv A

更新日期：2010-08-01 00:00:00