Abstract:
:Oxidative stress, characterized by the accumulation of reactive oxygen species (ROS), is known to have numerous detrimental effects on the myocardium such as the induction of apoptotic cell death, hypertrophy, fibrosis, dysfunction, and dilatation. Over the past several years, we have shown that mitochondrial NADP(+)-dependent isocitrate dehydrogenase (IDPm) functions as an antioxidant and anti-apoptotic protein by supplying NADPH to antioxidant systems. Here, we showed that transfection of H9c2 clonal myoblastic cells with small interfering RNA (siRNA) specific for IDPm markedly attenuated IDPm expression and substantially induced apoptosis, senescence, and hypertrophy as indicated by increased atrial natriuretic peptide (ANP) gene expression, a marker of cardiomyocyte hypertrophy, and a larger cell size. Knockdown of IDPm expression resulted in the modulation of cellular and mitochondrial redox status, mitochondrial function, and cellular oxidative damage. Taken together, our results suggest that the suppression of IDPm expression by siRNA induces apoptosis and hypertrophy of cultured cardiomyocytes through the disruption of cellular redox balance.
journal_name
Biochimiejournal_title
Biochimieauthors
Lee JH,Park JWdoi
10.1016/j.biochi.2013.11.016subject
Has Abstractpub_date
2014-04-01 00:00:00pages
110-8eissn
0300-9084issn
1638-6183pii
S0300-9084(13)00425-2journal_volume
99pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract::The considerable affinity of tissue transglutaminase for heparin was the basis for use of heparin-based affinity matrices for enzyme purification. Interaction of transglutaminase with heparin might mimic the physiological binding to membrane heparan sulfates, accounting for the limited but significant fraction of enzy...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.01.012
更新日期:2005-06-01 00:00:00
abstract::The mechanism of action on rabbit red cells of Treponema hyodysenteriae hemolysin was studied using volume analysis and release of hemoglobin. While fixation of the hemolysin on the erythrocytes is temperature independent, it appears that hemolysis is temperature dependent. The kinetics of hemolysis proceed according ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(80)80134-9
更新日期:1980-01-01 00:00:00
abstract::(-)-Epigallocatechin-3-gallate (EGCG), a well-known chemopreventive factor, induces cancer cells undergoing apoptosis. Over the last several years, we have shown that the mitochondrial NADP(+)-dependent isocitrate dehydrogenase (IDPm) functions as an antioxidant and anti-apoptotic protein by supplying NADPH to antioxi...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2011.06.025
更新日期:2011-10-01 00:00:00
abstract::All phytopathogenic fungi have two catalase-peroxidase paralogues located either intracellularly (KatG1) or extracellularly (KatG2). Here, for the first time a secreted bifunctional, homodimeric catalase-peroxidase (KatG2 from the rice blast fungus Magnaporthe grisea) has been produced heterologously with almost 100% ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2011.09.020
更新日期:2012-03-01 00:00:00
abstract::The ascomycete Cladosporium herbarum is a prominent fungal inducer of Type I allergy. The only major allergen identified so far is Cla h 8, a NADP-dependent mannitol dehydrogenase (MtDH). MtDH, a cytoplasmic protein of 28.5kDa, belongs to the Short chain Dehydrogenases/Reductases (SDR), acting as a NADP-dependent oxid...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.04.012
更新日期:2010-08-01 00:00:00
abstract::A previous study has shown the effect of individual mineral surfaces on the activity of sweet almond beta-D-glucosidase. We now consider more complex situations likely to occur in soil, such as adsorption onto mixtures of different mineral surfaces, and the effect on enzyme activity of mineral surfaces with organic co...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(87)90197-0
更新日期:1987-06-01 00:00:00
abstract::Complexes of purified 40S ribosomal subunits and initiation factor 3 from rabbit reticulocytes were crosslinked using the reversible protein crosslinking reagent, 2-iminothiolane, under conditions shown previously to lead to the formation of dimers between 40S proteins but not higher multimers. The activity of both th...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(83)80062-5
更新日期:1983-07-01 00:00:00
abstract::The dissociation of bound polyribosomes from ewe lactating mammary gland, by EDTA or Puromycin-KC1 treatment releases Messenger Ribonucleoprotein particles (mRNP'S) which sediment in sucrose gradients as a single component having a sedimentation coefficient of about 22S. RNAs derived from mRNPs sediment between 9S and...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(76)80439-7
更新日期:1976-01-01 00:00:00
abstract::The eukaryotic ribosomal protein S15 is a key component of the decoding site in contrast to its prokaryotic counterpart, S19p, which is located away from the mRNA binding track on the ribosome. Here, we determined the oligopeptide of S15 neighboring the A site mRNA codon on the human 80S ribosome with the use of mRNA ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.02.031
更新日期:2010-07-01 00:00:00
abstract::RNA editing alters genomically encoded cytidines to uridines posttranscriptionally in higher plant mitochondria. Most of these editing events occur in translated regions and consequently alter the amino acid sequence. In Oenothera berteriana more than 500 editing sites have been detected and the total number of editin...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(96)88108-9
更新日期:1995-01-01 00:00:00
abstract::Theoretical and experimental investigations on the conformations of synthetic polytripeptides, with Gly-R2-R3 general repetitive sequence, are of great interest for the comprehension of the factors which contribute to the triple helix stabilization. Using appropriate molecules as models, we have investigated by the PC...
journal_title:Biochimie
pub_type: 杂志文章
doi:
更新日期:1981-11-01 00:00:00
abstract::The iron absorption by duodenal enterocytes is a key step of its homeostasis. But the control of this absorption is complex and cannot be fully explicated with present knowledge. In a global transcriptome approach, we identified 60 genes over-expressed in hemin (iron) overload in Caco-2 cells, an in vitro model of duo...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2008.01.002
更新日期:2008-04-01 00:00:00
abstract::The structure difference between the free operator of the lac system d(GCTCACAAT).d(ATTGTGAGC) and the same operator complexed to the headpiece of the lac repressor has been investigated by 2-D-1H NMR spectroscopy in conjunction with molecular modelling in internal coordinates (JUMNA). The free and complexed operator ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(94)90006-x
更新日期:1994-01-01 00:00:00
abstract::Over-expression of efflux transporter P-glycoprotein (PgP) encoded by ABCB1 gene has been implicated in poor responsive epilepsy. Several genetic variants have been shown to influence the expression levels of P-glycoprotein. The aim of the present study was to investigate the role of ABCB1 polymorphisms: C1236T, G2677...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.04.008
更新日期:2010-09-01 00:00:00
abstract::Calprotectin (CP) is an abundant protein in human neutrophilic granulocytes and macrophages. In humans, serum, urine, and fecal concentrations of neutrophil-derived proteins, such as CP are used as markers of disease activity for conditions associated with increased neutrophil activity, such as inflammatory bowel dise...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2008.03.008
更新日期:2008-09-01 00:00:00
abstract::We have recently reported that α(2)β(1) and α(1)β(1) isozymes of Na(+)/K(+)-ATPase (NKA) are localized in the caveolae whereas only the α(1)β(1) isozyme of NKA is localized in the non-caveolae fraction of pulmonary artery smooth muscle cell membrane. It is well known that different isoforms of NKA are regulated differ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2011.12.020
更新日期:2012-04-01 00:00:00
abstract::A set of sequences can be defined by their common subsequences, and the length of these is a measure of the overall resemblance of the set. Each subsequence corresponds to a succession of symbols embedded in every sequence, following the same order but not necessarily contiguous. Determining the longest common subsequ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(85)80271-6
更新日期:1985-05-01 00:00:00
abstract::Lysosomal cysteine proteinases were fractionated from partially purified rat muscle lysosomes. By gel filtration on Sephadex G75, cathepsin D was separated from two thiol-requiring proteolytic fractions of Mr 25 000 and 55 000, respectively. By chromatofocusing, the first fraction (Mr = 25 000) was resolved into three...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(84)90114-7
更新日期:1984-09-01 00:00:00
abstract::Clustered apurinic/apyrimidinic (AP) sites are more cytotoxic than isolated AP lesions because double strand breaks (DSB) can be formed during repair of closely positioned bistranded AP sites. Formation of DSB due to simultaneous cleavage of bistranded AP sites may be regulated by proteins specifically interacting wit...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2018.04.027
更新日期:2018-07-01 00:00:00
abstract::Human matrilysin-1 (MMP-7) is one of the most potent elastases besides macrophage elastase in the family of matrix metalloproteinases (MMPs). It has been reported to provide macrophages with the highest elastinolytic capacity and plays key roles in diseases such as emphysema and cancer. Describing the enzymatic turnov...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.09.011
更新日期:2011-02-01 00:00:00
abstract::Specific contacts between DNA phosphate groups and positively charged nucleophilic amino acids from the Escherichia coli Fpg protein play a significant role in DNA-Fpg protein interaction. In order to identify these phosphate groups the chemical crosslinking procedure was carried out. The probing of the Fpg protein ac...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(03)00067-1
更新日期:2003-05-01 00:00:00
abstract::Unspliced human immunodeficiency virus-1 (HIV-1) mRNA is capped and therefore can be translated via conventional scanning mechanism. In addition, its 5' untranslated region (5'UTR) is thought to function as an internal ribosome entry site (IRES) during G2/M-phase of cell cycle or when cap-dependent translation is inhi...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2015.12.004
更新日期:2016-02-01 00:00:00
abstract::A variety of species of GPDH undergo acylation at two of the four active cystein sites per mole of tetrameric enzyme. This reaction requires tightly bound NAD+, a situation restricted to two of the four NAD sites per tetramer. S leads to N acyl transfer from cysteins to lysine in the diacyl enzyme yields an inactive e...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(81)80177-0
更新日期:1981-02-01 00:00:00
abstract::Halophilic proteins have evolved to be soluble, stable and active in high salt concentration. Crystallographic studies have shown that surface enrichment by acidic amino acids is a common structural feature of halophilic proteins. In addition, ion-binding sites have also been observed in most of the cases. The role of...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2007.03.008
更新日期:2007-08-01 00:00:00
abstract::Plasmalogens (Pls) represent a specific subclass of glycerophospholipids characterized by the presence of a vinyl-ether bond at the sn-1 position of glycerol. Pls are quantitatively important in membranes of neuronal tissues, including the brain and the retina, where they can represent until almost two-third of ethano...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2014.07.023
更新日期:2014-12-01 00:00:00
abstract::Fluorometry using a substrate DNA labeled with a single fluorophore (6-carboxyfluorescein) at the 3'-end of the processed strand was shown to be a useful tool for monitoring DNA-binding and 3'-processing activities of HIV-1 and PFV integrases (INs). The DNA binding to either of the INs resulted in a fluorescence signa...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.06.009
更新日期:2012-11-01 00:00:00
abstract::The eosinophil cationic protein (ECP) is a human antimicrobial protein involved in the host immune defense that belongs to the pancreatic RNase A family. ECP displays a wide range of antipathogen activities. The protein is highly cationic and its bactericidal activity is dependant on both cationic and hydrophobic surf...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.10.005
更新日期:2011-02-01 00:00:00
abstract::Gastrokine1 (GKN1), important for maintaining the physiological function of the gastric mucosa, is highly expressed in the stomach of healthy individuals but is down-regulated or absent in gastric tumor tissues and derived cell lines. The mechanisms underlying GKN1 gene inactivation are still unknown. We previously sh...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2019.09.005
更新日期:2019-12-01 00:00:00
abstract::Thyroid peroxidase is a member of a family of mammalian peroxidases that includes myeloperoxidase, lactoperoxidase, eosinophil peroxidase, and salivary peroxidase. Protein sequences showing a high degree of sequence similarity with mammalian peroxidases have recently been observed in several invertebrate species. A mu...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(99)80110-2
更新日期:1999-05-01 00:00:00
abstract::The binding properties of Mg2+, Mn2+ and Co2+ to yeast mitochondrial oligomycin sensitive ATPase complex are studied, as reflected by their catalytic effect (hydrolysis of ATP or pNPP, a pseudo substrate) or by a physical parameter (atomic absorption, electron paramagnetic reasonance of Mn2+, enhanced fluorescence of ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(78)80701-9
更新日期:1977-01-01 00:00:00