Abstract：
:GcvB is a non-coding RNA that regulates oppA mRNA in different bacterial species by binding a GcvB GU-rich region named R1 to oppA mRNA. A secondary putative interaction site (PS1) was identified in this study that is able to form a second nearly perfect 10 base-pair duplex between these two RNAs in Escherichia coli. In this work, we have studied whether the formation of a second interaction site could help stabilize the previously reported GcvB/oppA complex. Several mutations and the full deletion of PS1 were engineered. None of these modifications affected the ability of GcvB to control OppA expression. Therefore the second, putative, interaction site appears to be unnecessary for the regulatory function of GcvB with regard to its oppA target mRNA.
journal_name
Biochimiejournal_title
Biochimieauthors
Busi F,Le Derout J,Cerciat M,Régnier P,Hajnsdorf Edoi
10.1016/j.biochi.2010.06.020subject
Has Abstractpub_date
2010-10-01 00:00:00pages
1458-61issue
10eissn
0300-9084issn
1638-6183pii
S0300-9084(10)00251-8journal_volume
92pub_type
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