Abstract:
:Kinetic studies indicate that glucose-6-phosphatase is a multifunctional enzyme. a) Phosphohydrolase activities. The mannose-6-phosphatase activity is low (Km = 8 mM, VM = 90 nmoles. min-1mg-1). The enzyme shows a strong affinity for glucose-6-phosphate (Km = 2.5 mM, VM = 220 nmoles.min-1mg-1). beta-glycerophosphate (K1 = 30 mM), D-glucose (Ki = 120 mM) are mixed type inhibitors; pyrophosphate (Ki = 2 mM) is a non competitive one. b) Phosphotransferase activities. Di and triphosphate adenylic nucleosides or phosphoenol pyruvate are not substrates. Carbamylphosphate serves as a phosphoryl donor with D-glucose as acceptor. The phosphate transfer is consisstent with a random mechanism in which the binding of one substrate increases the enzymes affinity for the second substrate. Apparent Km values for carbamyl-phosphate range from 5.2 mM (D-glucose concentration leads to infinity) to 8 mM (D-glucose concentration leads to 0). The corresponding apparent Km values for D-glucose are 59 mM (carbamyl-phosphate concentration leads to infinity) to 119 mM (carbamyl-phosphate concentration leads to 0). Maximal reaction velocity with infinite levels of both substrates is 270 nmoles.min-1.mg-1. Pyrophosphate is a poor phosphoryl donnor (Km = 55 mM with D-glucose concentration 250 mM). In addition we do not find any latency; detergents, namely sodium deoxycholate, Triton X 100 do not affect or inhibit glucose-6-phosphatase activity.
journal_name
Biochimiejournal_title
Biochimieauthors
Benedetto JP,Martel MB,Got Rsubject
Has Abstractpub_date
1979-01-01 00:00:00pages
1125-32issue
10eissn
0300-9084issn
1638-6183journal_volume
61pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract::Four full-length cDNAs encoding the precursors of four K(+)-toxin-like peptides (named BmKK(1), BmKK(2), BmKK(3) and BmmKK(4), respectively) were first isolated from a venom gland cDNA library of the Chinese scorpion Buthus martensii Karsch. The deduced precursors of BmKK(1), BmKK(2) and BmKK(3) are all made of 54 ami...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(01)01326-8
更新日期:2001-09-01 00:00:00
abstract::Lysosomal cysteine proteinases were fractionated from partially purified rat muscle lysosomes. By gel filtration on Sephadex G75, cathepsin D was separated from two thiol-requiring proteolytic fractions of Mr 25 000 and 55 000, respectively. By chromatofocusing, the first fraction (Mr = 25 000) was resolved into three...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(84)90114-7
更新日期:1984-09-01 00:00:00
abstract::The suhB gene of Escherichia coli has been defined by its mutant allele that suppresses other mutants in secY, rpoH, dnaB, and era. The suhB mutant by itself is cold sensitive, and is shown to have defects in protein synthesis. Starting with the suhB cold-sensitive mutant, cold-resistant suppressors were isolated. The...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(96)88139-9
更新日期:1995-01-01 00:00:00
abstract::Trypsin and pronase treatment of purified human neutral bronchial mucins released small fragments from the C-terminal end of these molecules and resulted in slight increases in their sedimentation coefficient presumably reflecting conformational changes. The antigenic determinant of neutral bronchial mucins which appe...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(76)80444-0
更新日期:1976-01-01 00:00:00
abstract::Sialate 9(4)-O-acetylesterases (EC 3.1.1.53) have been isolated from equine liver, bovine brain and influenza C virus. In this latter case, the esterase represents the receptor-destroying enzyme of the virus. The kinetic properties of these enzymes were determined with Neu5,9Ac2 and in part with 4-methylumbelliferyl a...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90288-x
更新日期:1988-11-01 00:00:00
abstract::The presence of marker genes encoding antibiotic or herbicide resistances in genetically modified plants poses a number of problems. Various techniques are under development for the removal of unwanted marker genes, while leaving required transgenes in place. The aim of this brief review is to describe the principal m...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(02)00021-4
更新日期:2002-11-01 00:00:00
abstract::Phospholipid analysis of the membranes associated with fast sedimenting folded chromosomes prepared by lysis of E. coli CR 34 shows that both inner and outer membranes are parts of the complex, in proportions not very different from that found in the whole bacteria. During the preparation of the folded chromosomes, th...
journal_title:Biochimie
pub_type: 杂志文章
doi:
更新日期:1977-01-01 00:00:00
abstract::Matrix metalloproteinase 9 (MMP-9) and interleukin-8 (IL-8) play major roles in tumor progression and invasion of breast cancer cells. The present study was undertaken to investigate the inhibitory mechanism of cell invasion by luteolin 8-C-β-fucopyranoside (named as LU8C-FP), a C-glycosylflavone, in human breast canc...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.07.021
更新日期:2013-11-01 00:00:00
abstract::The action of sialyltransferases (STs) on cell surface glycoconjugates is a key process in shaping cell phenotype in a variety of cells mostly involved in migratory and adhesive pathways. The factors determining cell-specific pattern of glycosylation are so far poorly understood. Most STs are resident proteins of the ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(03)00080-4
更新日期:2003-03-01 00:00:00
abstract::Replication protein A (RPA) is a heterotrimeric single-stranded DNA binding protein whose role in DNA replication, recombination and repair has been mainly elucidated through in vitro biochemical studies utilizing the mammalian complex. However, the identification of homologs of all three subunits in Saccharomyces cer...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(00)00183-8
更新日期:2000-01-01 00:00:00
abstract::A key target in the treatment of HIV-1/AIDS has been the viral protease. Here we first studied in silico the evolution of protease resistance. Primary active site resistance mutations were found to weaken interactions between protease and both inhibitor and substrate P4-P4' residues. We next studied the effects of sec...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2015.08.009
更新日期:2015-11-01 00:00:00
abstract::MicroRNAs (miRNAs) play profound roles in plant growth and development by regulating gene expression. Tetraploid plants often have better physical characteristics and stress tolerance than their diploid progenitors, but the role of miRNAs in this superiority is unclear. Paulownia tomentosa, (Paulowniaceae) is attracti...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.02.008
更新日期:2014-07-01 00:00:00
abstract::When liver cells from either normal or hereditarily diabetic rats are exposed to (13)C-enriched D-fructose (10 mM) and unlabelled D-glucose (also 10 mM) in the presence of D(2)O, the output of (13)C-enriched D-glucose generated from D-[1-(13)C]fructose is significantly lower than that from D-[2-(13)C]fructose. This co...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2003.12.005
更新日期:2004-02-01 00:00:00
abstract::Two soluble forms of brain glutamate dehydrogenase isoproteins were inactivated by pyridoxal 5'-phosphate. Restoration of catalytic activity can be accomplished by dialysis and addition of an excess of cysteine or lysine. Spectral evidence is presented to indicate that the inactivation proceeds through Schiff base for...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(97)84333-7
更新日期:1996-01-01 00:00:00
abstract::The lysosomal aspartic protease cathepsin D (cath-D) is overexpressed and hyper-secreted by epithelial breast cancer cells. This protease is an independent marker of poor prognosis in breast cancer as it is correlated with the incidence of clinical metastasis. In normal cells, cath-D is localized in intracellular vesi...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2010.05.009
更新日期:2010-11-01 00:00:00
abstract::In the present study we evaluated the effect of chronic methionine administration on oxidative stress and biochemical parameters in liver and serum of rats, respectively. We also performed histological analysis in liver. Results showed that hypermethioninemia increased chemiluminescence, carbonyl content and glutathio...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2009.04.018
更新日期:2009-08-01 00:00:00
abstract::The possibility that brown adipose tissue thermogenesis can be recruited in order to combat the development of obesity has led to a high interest in the identification of "browning agents", i.e. agents that increase the amount and activity of UCP1 in brown and brite/beige adipose tissues. However, functional analysis ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2017.01.007
更新日期:2017-03-01 00:00:00
abstract::Oxazole-containing macrocycles, which include the natural product telomestatin, represent a promising class of anticancer agents that target G-quadruplex DNA. Two synthetic hexaoxazole-containing macrocyclic compounds (HXDV and HXLV-AC) have been characterized with regard to their cytotoxic activities versus human can...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2008.03.011
更新日期:2008-08-01 00:00:00
abstract::Cell sterol supply is subjected to tight negative feedback regulation through the SREBP pathway. Upon cholesterol depletion, SREBP transcription factors become activated by cleavage of a membrane bound precursor form, which stimulates the expression of the genes encoding proteins of the cholesterol synthesis pathway. ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2006.09.015
更新日期:2007-02-01 00:00:00
abstract::Classic studies suggested that the common mammalian sialic acid N-glycolylneuraminic acid (Neu5Gc) is an oncofetal antigen in humans, being immunogenic in adult humans and yet apparently expressed in human fetuses and tumors. We and others have recently found that the human deficiency of Neu5Gc can be explained by an ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(01)01309-8
更新日期:2001-07-01 00:00:00
abstract::P53 is a transcription factor also called the "cellular gatekeeper of genome" because it can induce cell cycle arrest in G1, apoptosis or affect DNA replication in response to DNA damage. Wild type p53 is localised in both the cytoplasm and nucleus of first trimester trophoblastic cells (CTB). Immunoblotting of CTB wi...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2008.02.006
更新日期:2008-06-01 00:00:00
abstract::Seven virulent bacteriophages of Streptococcus thermophilus were characterized at the molecular level and classified into 2 subgroups (A and B) by DNA/DNA hybridization experiments and analysis of their structural proteins. Two representatives of subgroups A and B were compared to 3 representatives of Neve's subgroups...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(90)90002-x
更新日期:1990-12-01 00:00:00
abstract::Translational control is a common mechanism used to regulate gene expression and occur in bacteria to mammals. Typically in translational control, an RNA-binding protein binds to a unique sequence in the mRNA to regulate protein synthesis by the ribosomes. Alternatively, a protein may bind to or modify a translation f...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2015.02.005
更新日期:2015-07-01 00:00:00
abstract::High-mobility group box-1 (HMGB1) is remarkably mobile in living cells, which reflects its ability to interact only transiently with both DNA and protein. This property is likely essential for HMGB1 nuclear activities. Nonetheless the weak interaction of HMGB1 with DNA and/or protein partners has also been a major lim...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.11.001
更新日期:2013-04-01 00:00:00
abstract::Secondary structure-forming DNA motifs have achieved infamy because of their association with a variety of human diseases and cancer. The 3rd FASEB summer conference on dynamic DNA structures focused on the mechanisms responsible for the instabilities inherent to repetitive DNA and presented many exciting and novel as...
journal_title:Biochimie
pub_type:
doi:10.1016/j.biochi.2012.10.005
更新日期:2013-02-01 00:00:00
abstract::Here we report two novel 17-mer amidated linear peptides (TsAP-1 and TsAP-2) whose structures were deduced from cDNAs cloned from a venom-derived cDNA library of the Brazilian yellow scorpion, Tityus serrulatus. Both mature peptides were structurally-characterised following their location in chromatographic fractions ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.06.003
更新日期:2013-09-01 00:00:00
abstract::Gastrokine1 (GKN1), important for maintaining the physiological function of the gastric mucosa, is highly expressed in the stomach of healthy individuals but is down-regulated or absent in gastric tumor tissues and derived cell lines. The mechanisms underlying GKN1 gene inactivation are still unknown. We previously sh...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2019.09.005
更新日期:2019-12-01 00:00:00
abstract::The present review deals with the place of single chain oligonucleotide ligands (aptamers) in affinity chromatography applied to proteins. Aptamers are not the only affinity ligands available but they represent an emerging and highly promising route that advantageously competes with antibodies in immunopurification pr...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2017.10.008
更新日期:2018-02-01 00:00:00
abstract::Phospholipases A2 (PLA2) are a family of enzymes that catalyze the hydrolysis of the sn-2 ester bond of glycerophospholipids liberating lysophospholipids and free fatty acids; important second messengers involved in atherogenesis. Plasma PAF-acetylhydrolase (PAF-AH) or Lp-PLA2 is a Ca(2+)-independent PLA2 which is pro...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2010.02.002
更新日期:2010-06-01 00:00:00
abstract::Various phosphonic and sulfonic glutamate analogues as well as phosphonopeptides related to glutathione were studied for their interaction with rat kidney gamma-glutamylcysteine synthetase activity. We found, in all cases, that the presence of a phosphonic group increases the affinity for the enzyme. Among the tripept...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(87)90083-6
更新日期:1987-05-01 00:00:00